Sewage-associated circular DNA virus-1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J166|A0A075J166_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-1 OX=1519385 PE=3 SV=1
MM1 pKa = 7.3PPAWEE6 pKa = 4.56GSSFIITYY14 pKa = 7.81PQSDD18 pKa = 4.0FDD20 pKa = 3.95LQEE23 pKa = 5.24YY24 pKa = 10.05IDD26 pKa = 3.29TWEE29 pKa = 4.04NKK31 pKa = 8.53KK32 pKa = 10.17HH33 pKa = 5.99IKK35 pKa = 9.9YY36 pKa = 10.6LLISTEE42 pKa = 3.93LHH44 pKa = 5.61QDD46 pKa = 3.26EE47 pKa = 4.91SLHH50 pKa = 5.4RR51 pKa = 11.84HH52 pKa = 5.19AVLHH56 pKa = 6.02FSKK59 pKa = 10.33KK60 pKa = 7.89QRR62 pKa = 11.84VGHH65 pKa = 6.56AFFDD69 pKa = 3.57HH70 pKa = 6.73LTRR73 pKa = 11.84HH74 pKa = 6.03PNIKK78 pKa = 10.2CVGKK82 pKa = 10.49RR83 pKa = 11.84RR84 pKa = 11.84SDD86 pKa = 3.24WTNVIAYY93 pKa = 7.95VKK95 pKa = 10.28KK96 pKa = 10.48CGEE99 pKa = 3.95FVEE102 pKa = 4.64WGEE105 pKa = 4.23PRR107 pKa = 11.84HH108 pKa = 5.81SACVWSAIATSSTRR122 pKa = 11.84QEE124 pKa = 4.04AQEE127 pKa = 3.91MLSIEE132 pKa = 4.32RR133 pKa = 11.84PRR135 pKa = 11.84DD136 pKa = 3.2AVLNARR142 pKa = 11.84NFDD145 pKa = 3.38YY146 pKa = 10.71WLDD149 pKa = 3.49KK150 pKa = 10.3VFPMSEE156 pKa = 3.92TASYY160 pKa = 10.29SGRR163 pKa = 11.84SSDD166 pKa = 3.73QFVVPPALQDD176 pKa = 3.34WVLMSWWYY184 pKa = 9.9VLPRR188 pKa = 11.84QEE190 pKa = 4.18RR191 pKa = 11.84PKK193 pKa = 11.15SLVLNGASRR202 pKa = 11.84LGKK205 pKa = 6.84TQWARR210 pKa = 11.84SLGPHH215 pKa = 6.66IYY217 pKa = 9.57IGNMWDD223 pKa = 3.87LGALDD228 pKa = 5.84GIDD231 pKa = 3.68SNFWNYY237 pKa = 10.37GYY239 pKa = 10.34IVFDD243 pKa = 4.93DD244 pKa = 4.53IEE246 pKa = 4.2WASIAGSAKK255 pKa = 10.01SWFGAQRR262 pKa = 11.84DD263 pKa = 3.88FSVSDD268 pKa = 3.18KK269 pKa = 10.74YY270 pKa = 11.01KK271 pKa = 10.71RR272 pKa = 11.84RR273 pKa = 11.84TGWWYY278 pKa = 8.65VTSTNEE284 pKa = 3.66NGDD287 pKa = 3.64WLVVFLAFFLSTLTLIAVIVIVLLTQNGDD316 pKa = 3.32VKK318 pKa = 10.25TLII321 pKa = 4.29

Molecular weight: 37.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J166|A0A075J166_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-1 OX=1519385 PE=3 SV=1
MM1 pKa = 7.46SKK3 pKa = 11.22YY4 pKa = 7.4MTPKK8 pKa = 10.14NVARR12 pKa = 11.84VINFAQQAKK21 pKa = 8.4RR22 pKa = 11.84TWDD25 pKa = 3.53SPGMRR30 pKa = 11.84TVRR33 pKa = 11.84AAGKK37 pKa = 9.58AARR40 pKa = 11.84SRR42 pKa = 11.84IAANRR47 pKa = 11.84LRR49 pKa = 11.84GKK51 pKa = 9.75KK52 pKa = 9.87SKK54 pKa = 10.77GFGGPSSGHH63 pKa = 4.26YY64 pKa = 9.72VGPFKK69 pKa = 10.74RR70 pKa = 11.84PKK72 pKa = 9.21PVKK75 pKa = 9.49KK76 pKa = 10.58NKK78 pKa = 9.54IADD81 pKa = 3.72YY82 pKa = 10.42LSQGVCEE89 pKa = 4.18YY90 pKa = 11.23KK91 pKa = 10.39EE92 pKa = 3.59IHH94 pKa = 6.66GNVSDD99 pKa = 4.73PDD101 pKa = 3.63CVYY104 pKa = 10.77ATFSTWDD111 pKa = 3.27DD112 pKa = 3.86SMVEE116 pKa = 3.98RR117 pKa = 11.84QLYY120 pKa = 9.44GALLRR125 pKa = 11.84KK126 pKa = 9.22LLKK129 pKa = 10.37KK130 pKa = 10.68AGINIDD136 pKa = 4.12RR137 pKa = 11.84NDD139 pKa = 3.29QVLPMSQYY147 pKa = 10.06NNSGGTLIQAVFKK160 pKa = 10.52SVSGSVLIYY169 pKa = 10.72EE170 pKa = 4.85DD171 pKa = 4.45LLGGAQSIQSIVSQDD186 pKa = 3.91LIKK189 pKa = 10.94VGLWFKK195 pKa = 11.56VMVKK199 pKa = 10.1GQQSLTFPDD208 pKa = 3.09QSYY211 pKa = 10.23QLEE214 pKa = 4.29SLSMYY219 pKa = 9.52GQSTTHH225 pKa = 5.61SAEE228 pKa = 4.1RR229 pKa = 11.84HH230 pKa = 5.88CLAQLNVMQEE240 pKa = 3.95VLQIEE245 pKa = 4.58MFLEE249 pKa = 3.94ISIQNRR255 pKa = 11.84TKK257 pKa = 10.88GAIAGTGDD265 pKa = 3.53TEE267 pKa = 4.19TDD269 pKa = 3.4RR270 pKa = 11.84VDD272 pKa = 3.5SQPLQGKK279 pKa = 9.62LYY281 pKa = 8.91EE282 pKa = 4.47FNGGVPKK289 pKa = 10.28EE290 pKa = 3.99RR291 pKa = 11.84QKK293 pKa = 11.85GNGLLTEE300 pKa = 4.27VEE302 pKa = 4.2ALGISLNRR310 pKa = 11.84AAQIGNEE317 pKa = 4.3YY318 pKa = 10.51KK319 pKa = 10.42EE320 pKa = 4.44PPPAKK325 pKa = 10.04HH326 pKa = 6.02FSNVSKK332 pKa = 10.84VANQKK337 pKa = 9.05LQPGSIKK344 pKa = 10.11RR345 pKa = 11.84HH346 pKa = 4.37YY347 pKa = 9.46LKK349 pKa = 9.83WYY351 pKa = 10.46ARR353 pKa = 11.84GFVNNLVNRR362 pKa = 11.84WSSKK366 pKa = 8.1QNPTFGQTVVASPGKK381 pKa = 9.19CAMFAFEE388 pKa = 4.76EE389 pKa = 4.44VLNSGSSNLITVSYY403 pKa = 10.38EE404 pKa = 3.59VDD406 pKa = 3.35KK407 pKa = 11.56KK408 pKa = 11.15CGAKK412 pKa = 10.54LITAKK417 pKa = 10.28AAPMKK422 pKa = 10.05PLYY425 pKa = 10.46TSDD428 pKa = 3.97TYY430 pKa = 11.56SSPP433 pKa = 3.29

Molecular weight: 47.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

754

321

433

377.0

42.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.029 ± 0.296

1.061 ± 0.077

4.775 ± 0.886

4.509 ± 0.1

3.979 ± 0.612

7.162 ± 0.946

2.122 ± 0.604

5.172 ± 0.454

7.029 ± 1.244

8.09 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.343

4.642 ± 0.55

4.244 ± 0.308

5.172 ± 0.872

5.04 ± 0.345

9.151 ± 0.071

4.775 ± 0.128

7.162 ± 0.002

2.918 ± 1.447

3.846 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski