Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella; Brucella anthropi

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6X638|A6X638_OCHA4 Isochorismatase hydrolase OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=Oant_3987 PE=4 SV=1
MM1 pKa = 8.0PIPRR5 pKa = 11.84VSDD8 pKa = 3.95TIHH11 pKa = 6.95LSDD14 pKa = 5.13VYY16 pKa = 11.28AGDD19 pKa = 4.14ANAGNGADD27 pKa = 4.31GYY29 pKa = 11.63NFGNINYY36 pKa = 9.09NPSAVVANTQNVTGAAVDD54 pKa = 3.61VHH56 pKa = 8.14SGDD59 pKa = 4.47SPWLNASWDD68 pKa = 3.63AGHH71 pKa = 7.23AGDD74 pKa = 5.67GGGADD79 pKa = 3.43ATNGFLANITNNGAGGAGGDD99 pKa = 3.75ANSNGSLGSSSGGNVAAGQADD120 pKa = 4.13TSAVQYY126 pKa = 10.67TEE128 pKa = 5.14LLADD132 pKa = 3.19QHH134 pKa = 5.73ATILAGVGGNGGDD147 pKa = 3.34GNMARR152 pKa = 11.84GGDD155 pKa = 3.26ISSALVHH162 pKa = 6.42SNPEE166 pKa = 4.05TTTTTTTTTTNDD178 pKa = 2.68IANFIDD184 pKa = 3.69SFNDD188 pKa = 3.05NFHH191 pKa = 7.58DD192 pKa = 4.12IDD194 pKa = 4.98LSHH197 pKa = 7.2LPLL200 pKa = 5.43

Molecular weight: 19.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6WYP6|A6WYP6_OCHA4 Aa3 type cytochrome c oxidase subunit IV OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=Oant_1383 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATTGGRR28 pKa = 11.84KK29 pKa = 9.17VLTARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.96RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4757

0

4757

1495400

37

2906

314.4

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.278 ± 0.043

0.779 ± 0.011

5.645 ± 0.03

5.884 ± 0.037

3.961 ± 0.025

8.247 ± 0.049

1.975 ± 0.016

5.931 ± 0.025

4.135 ± 0.029

9.906 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.016

3.141 ± 0.026

4.728 ± 0.026

3.237 ± 0.021

6.447 ± 0.037

5.836 ± 0.029

5.348 ± 0.028

7.271 ± 0.025

1.275 ± 0.014

2.393 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski