Gleimia coleocanis DSM 15436

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Gleimia; Gleimia coleocanis

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1546 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0VZB9|C0VZB9_9ACTO Antigen 84 OS=Gleimia coleocanis DSM 15436 OX=525245 GN=HMPREF0044_0509 PE=3 SV=1
MM1 pKa = 7.56SFQLACPADD10 pKa = 4.2LTPGSARR17 pKa = 11.84AVNLTDD23 pKa = 4.48ASGTNHH29 pKa = 6.72LVAIVCDD36 pKa = 4.22TEE38 pKa = 4.96GEE40 pKa = 4.1WFAIDD45 pKa = 3.96DD46 pKa = 4.42TCTHH50 pKa = 6.79ADD52 pKa = 3.44VPLSEE57 pKa = 4.49GDD59 pKa = 3.15IGAGCIEE66 pKa = 4.69CWAHH70 pKa = 6.31SAEE73 pKa = 4.11FDD75 pKa = 3.47LRR77 pKa = 11.84TGEE80 pKa = 4.36GSFPAPKK87 pKa = 9.73PVRR90 pKa = 11.84TYY92 pKa = 10.9PLTIEE97 pKa = 4.15NQQVLVDD104 pKa = 3.59VDD106 pKa = 3.46NN107 pKa = 4.35

Molecular weight: 11.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0VY28|C0VY28_9ACTO Class I glutamine amidotransferase OS=Gleimia coleocanis DSM 15436 OX=525245 GN=HMPREF0044_0068 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 8.58THH17 pKa = 5.46GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.82GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1546

0

1546

517147

32

2453

334.5

36.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.806 ± 0.068

0.739 ± 0.018

5.127 ± 0.044

6.586 ± 0.064

3.713 ± 0.043

7.725 ± 0.057

1.965 ± 0.024

5.351 ± 0.046

4.188 ± 0.047

10.277 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.029

3.323 ± 0.049

4.785 ± 0.042

3.631 ± 0.035

5.345 ± 0.054

5.721 ± 0.047

6.261 ± 0.055

8.448 ± 0.061

1.404 ± 0.025

2.476 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski