Glossina hytrovirus (isolate Glossina pallidipes/Ethiopia/Seibersdorf/-) (GHV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Hytrosaviridae; Glossinavirus; Glossina hytrosavirus

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0YLV6|B0YLV6_GHVS Uncharacterized protein OS=Glossina hytrovirus (isolate Glossina pallidipes/Ethiopia/Seibersdorf/-) OX=379529 GN=SGHV152 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 4.91EE3 pKa = 4.22RR4 pKa = 11.84YY5 pKa = 10.21EE6 pKa = 4.57LISKK10 pKa = 10.09LADD13 pKa = 3.33LFIAYY18 pKa = 10.1RR19 pKa = 11.84NVLFYY24 pKa = 11.31KK25 pKa = 9.79MQLCEE30 pKa = 4.07RR31 pKa = 11.84EE32 pKa = 4.17NNEE35 pKa = 3.92DD36 pKa = 3.79VYY38 pKa = 11.65SLEE41 pKa = 4.34EE42 pKa = 4.2VNSTRR47 pKa = 11.84YY48 pKa = 9.75NLSSEE53 pKa = 4.19QEE55 pKa = 4.38SVEE58 pKa = 4.02EE59 pKa = 4.01ALMLLRR65 pKa = 11.84QYY67 pKa = 10.11EE68 pKa = 4.42HH69 pKa = 7.61YY70 pKa = 10.84EE71 pKa = 4.19DD72 pKa = 5.51IIDD75 pKa = 4.88GLITDD80 pKa = 4.92PLL82 pKa = 4.81

Molecular weight: 9.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0YLU1|B0YLU1_GHVS Uncharacterized protein OS=Glossina hytrovirus (isolate Glossina pallidipes/Ethiopia/Seibersdorf/-) OX=379529 GN=SGHV137 PE=4 SV=1
MM1 pKa = 7.62IKK3 pKa = 10.46LEE5 pKa = 3.82QARR8 pKa = 11.84RR9 pKa = 11.84SIAKK13 pKa = 9.57YY14 pKa = 9.98PIMSTKK20 pKa = 10.71NKK22 pKa = 9.95DD23 pKa = 2.83KK24 pKa = 10.51RR25 pKa = 11.84YY26 pKa = 8.72YY27 pKa = 9.87ISAIQYY33 pKa = 8.15VSQCRR38 pKa = 11.84FYY40 pKa = 11.38NLKK43 pKa = 8.47TLSSIISPSSKK54 pKa = 10.08HH55 pKa = 5.39GHH57 pKa = 6.24LEE59 pKa = 3.86NNRR62 pKa = 11.84SHH64 pKa = 6.85SFF66 pKa = 3.18

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

160

0

160

54662

52

4373

341.6

40.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.728 ± 0.095

1.879 ± 0.177

5.604 ± 0.158

6.344 ± 0.771

5.481 ± 0.342

2.532 ± 0.134

2.146 ± 0.133

10.307 ± 0.266

8.736 ± 0.297

9.504 ± 0.186

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.099

9.509 ± 0.252

3.222 ± 0.127

3.924 ± 0.375

3.439 ± 0.129

6.43 ± 0.145

5.441 ± 0.123

4.564 ± 0.111

0.673 ± 0.071

5.15 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski