Beihai barnacle virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLA3|A0A1L3KLA3_9VIRU RdRp OS=Beihai barnacle virus 12 OX=1922356 PE=4 SV=1
MM1 pKa = 7.74LSLQPGEE8 pKa = 4.58SFRR11 pKa = 11.84QDD13 pKa = 2.87FRR15 pKa = 11.84NPGRR19 pKa = 11.84DD20 pKa = 3.27KK21 pKa = 11.29LATEE25 pKa = 4.61ALNHH29 pKa = 6.35AFGSDD34 pKa = 3.22RR35 pKa = 11.84VDD37 pKa = 3.96KK38 pKa = 10.98ILATLHH44 pKa = 6.26RR45 pKa = 11.84AEE47 pKa = 4.83KK48 pKa = 10.89SLDD51 pKa = 3.48EE52 pKa = 5.07LIADD56 pKa = 3.37VMLFDD61 pKa = 4.74RR62 pKa = 11.84EE63 pKa = 4.34RR64 pKa = 11.84VGPAARR70 pKa = 11.84DD71 pKa = 3.56DD72 pKa = 4.08PVYY75 pKa = 10.73QLALEE80 pKa = 4.31SVRR83 pKa = 11.84QEE85 pKa = 4.49FIPKK89 pKa = 6.21EE90 pKa = 4.26TKK92 pKa = 10.4VIPLTLGGVEE102 pKa = 4.48NRR104 pKa = 11.84PDD106 pKa = 3.37LPRR109 pKa = 11.84NKK111 pKa = 10.42SPGLPLNEE119 pKa = 3.94HH120 pKa = 6.79FKK122 pKa = 10.84TKK124 pKa = 10.81GEE126 pKa = 4.05ALSDD130 pKa = 3.94PGVHH134 pKa = 7.34ADD136 pKa = 3.61IHH138 pKa = 6.67KK139 pKa = 9.99KK140 pKa = 8.22WALIGKK146 pKa = 8.73GYY148 pKa = 9.19KK149 pKa = 9.48QKK151 pKa = 10.83LHH153 pKa = 7.24DD154 pKa = 3.84SALFQRR160 pKa = 11.84AQICSTEE167 pKa = 4.03KK168 pKa = 10.57NKK170 pKa = 9.4IRR172 pKa = 11.84AVWGYY177 pKa = 8.1PLEE180 pKa = 4.31VFMEE184 pKa = 4.67EE185 pKa = 4.04ARR187 pKa = 11.84FFYY190 pKa = 10.06PYY192 pKa = 9.34MDD194 pKa = 5.19YY195 pKa = 10.81ILSCAQNLPIGYY207 pKa = 8.24QAEE210 pKa = 4.34MATGGMLYY218 pKa = 9.86INDD221 pKa = 4.6MIRR224 pKa = 11.84SHH226 pKa = 6.5PHH228 pKa = 4.43ATFAICDD235 pKa = 3.35WSRR238 pKa = 11.84FDD240 pKa = 3.4KK241 pKa = 10.52TVPAWLIRR249 pKa = 11.84DD250 pKa = 4.24AFQIILDD257 pKa = 4.02SLDD260 pKa = 3.35LSKK263 pKa = 11.44VQGSDD268 pKa = 3.08GAIWNVRR275 pKa = 11.84EE276 pKa = 3.97EE277 pKa = 4.12DD278 pKa = 3.24SLRR281 pKa = 11.84RR282 pKa = 11.84FKK284 pKa = 10.9RR285 pKa = 11.84VVSYY289 pKa = 8.48FINTPIRR296 pKa = 11.84SPDD299 pKa = 2.78GSRR302 pKa = 11.84FRR304 pKa = 11.84KK305 pKa = 9.95RR306 pKa = 11.84GGVPSGSAFTNIIDD320 pKa = 4.27SIVNAIVTRR329 pKa = 11.84YY330 pKa = 9.11LAYY333 pKa = 9.8HH334 pKa = 6.96CSGALPSADD343 pKa = 3.8IYY345 pKa = 11.42LGDD348 pKa = 4.91DD349 pKa = 3.55SVCVVNGVVNLQDD362 pKa = 3.19WADD365 pKa = 3.69LARR368 pKa = 11.84EE369 pKa = 4.06KK370 pKa = 10.94FSMEE374 pKa = 3.96LNVEE378 pKa = 3.95KK379 pKa = 10.7SYY381 pKa = 10.1VTTNPCNVHH390 pKa = 5.57FLGYY394 pKa = 10.49FNLQGLPIKK403 pKa = 10.74GQDD406 pKa = 3.01FAMASFLYY414 pKa = 9.78PEE416 pKa = 4.89RR417 pKa = 11.84KK418 pKa = 9.58SLSPTITAARR428 pKa = 11.84ALGQMWSTMNPYY440 pKa = 9.97AASNWHH446 pKa = 6.73DD447 pKa = 3.51VVTYY451 pKa = 10.92IMDD454 pKa = 3.96TQDD457 pKa = 2.93VTLSQIQEE465 pKa = 4.15HH466 pKa = 6.47LRR468 pKa = 11.84SQPEE472 pKa = 3.66AFRR475 pKa = 11.84YY476 pKa = 9.53LRR478 pKa = 11.84MMGLDD483 pKa = 3.47VATLGLPRR491 pKa = 11.84KK492 pKa = 9.85VDD494 pKa = 3.54GLVLEE499 pKa = 4.3VQPRR503 pKa = 11.84SVSRR507 pKa = 11.84KK508 pKa = 7.21IYY510 pKa = 10.41KK511 pKa = 9.9VVTHH515 pKa = 7.26DD516 pKa = 3.42VTKK519 pKa = 10.89LMSLAIDD526 pKa = 4.6RR527 pKa = 11.84IEE529 pKa = 4.44TDD531 pKa = 3.38LQEE534 pKa = 4.31QDD536 pKa = 4.3DD537 pKa = 4.42VPEE540 pKa = 4.58EE541 pKa = 4.11EE542 pKa = 5.4DD543 pKa = 4.47DD544 pKa = 3.71NN545 pKa = 4.67

Molecular weight: 61.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLA3|A0A1L3KLA3_9VIRU RdRp OS=Beihai barnacle virus 12 OX=1922356 PE=4 SV=1
MM1 pKa = 7.88SSRR4 pKa = 11.84QTQEE8 pKa = 4.82DD9 pKa = 3.98NQPDD13 pKa = 4.01EE14 pKa = 4.55PVPNPTEE21 pKa = 3.59QKK23 pKa = 9.13TRR25 pKa = 11.84VFSNSGRR32 pKa = 11.84RR33 pKa = 11.84GKK35 pKa = 9.66PKK37 pKa = 9.72PQSKK41 pKa = 10.17AGKK44 pKa = 8.37PKK46 pKa = 10.41VSAGPKK52 pKa = 9.34APPPAGPPKK61 pKa = 9.46PQHH64 pKa = 6.4LSGKK68 pKa = 8.14GTSLANDD75 pKa = 4.49PLWQEE80 pKa = 4.09AFVLEE85 pKa = 4.2GDD87 pKa = 3.38KK88 pKa = 11.28GYY90 pKa = 10.95VQVPTKK96 pKa = 10.75RR97 pKa = 11.84EE98 pKa = 3.92FQVSAEE104 pKa = 4.04GLHH107 pKa = 7.01DD108 pKa = 3.73IASAVYY114 pKa = 9.44TEE116 pKa = 4.07MSMVDD121 pKa = 2.54GHH123 pKa = 5.85FRR125 pKa = 11.84KK126 pKa = 8.58TIPEE130 pKa = 4.32SAFHH134 pKa = 6.76YY135 pKa = 7.64YY136 pKa = 10.27TIVLLHH142 pKa = 6.64ARR144 pKa = 11.84LAEE147 pKa = 4.12LGKK150 pKa = 10.24RR151 pKa = 11.84HH152 pKa = 5.96PTDD155 pKa = 2.78TTYY158 pKa = 11.52EE159 pKa = 3.9DD160 pKa = 3.68FRR162 pKa = 11.84FVEE165 pKa = 4.29LMKK168 pKa = 10.57EE169 pKa = 4.03YY170 pKa = 10.97GPVPEE175 pKa = 5.37PIEE178 pKa = 4.14AYY180 pKa = 9.82FRR182 pKa = 11.84SIGDD186 pKa = 3.79LPDD189 pKa = 3.38VAGVEE194 pKa = 4.58YY195 pKa = 10.69KK196 pKa = 10.82LKK198 pKa = 10.87LPPKK202 pKa = 9.36PLADD206 pKa = 3.17RR207 pKa = 11.84HH208 pKa = 5.7GPSTGFHH215 pKa = 5.85RR216 pKa = 11.84RR217 pKa = 11.84VSAEE221 pKa = 3.73THH223 pKa = 5.12NTWEE227 pKa = 4.17QVPAPGVAAMRR238 pKa = 11.84MCQDD242 pKa = 2.94MAYY245 pKa = 7.7TQHH248 pKa = 7.28PNRR251 pKa = 11.84DD252 pKa = 3.28IGPIWDD258 pKa = 4.45LPAEE262 pKa = 4.05LRR264 pKa = 11.84PVPEE268 pKa = 5.2HH269 pKa = 5.78GHH271 pKa = 5.06TPGRR275 pKa = 11.84PTGNLLGWQPATLLSNEE292 pKa = 3.76QRR294 pKa = 11.84TSLSQCGVTPTEE306 pKa = 3.96ASTAGTYY313 pKa = 10.01AVNLQVLGMVSSALARR329 pKa = 11.84ATSVYY334 pKa = 9.61RR335 pKa = 11.84CASAAVHH342 pKa = 6.06NSANGSYY349 pKa = 10.48AQATFCEE356 pKa = 4.7VEE358 pKa = 4.37DD359 pKa = 4.34SDD361 pKa = 6.1LGFQRR366 pKa = 11.84RR367 pKa = 11.84QQYY370 pKa = 8.83TSAGLRR376 pKa = 11.84SCTAFQADD384 pKa = 3.36ARR386 pKa = 11.84ISVASRR392 pKa = 11.84NCCFRR397 pKa = 11.84VKK399 pKa = 10.38RR400 pKa = 11.84KK401 pKa = 8.26TGADD405 pKa = 3.28GTNRR409 pKa = 11.84WACYY413 pKa = 10.19DD414 pKa = 3.46YY415 pKa = 11.15DD416 pKa = 3.77QYY418 pKa = 11.18RR419 pKa = 11.84TVPPSWTEE427 pKa = 3.61NMNATFNAGEE437 pKa = 4.2ASAEE441 pKa = 3.99LNITKK446 pKa = 10.36LATAYY451 pKa = 10.33ADD453 pKa = 3.32KK454 pKa = 11.12SRR456 pKa = 11.84TLRR459 pKa = 11.84PWARR463 pKa = 11.84APVINKK469 pKa = 9.39SRR471 pKa = 11.84DD472 pKa = 3.13

Molecular weight: 51.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1017

472

545

508.5

56.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.948 ± 0.998

1.377 ± 0.193

6.293 ± 0.99

5.605 ± 0.07

3.638 ± 0.408

6.195 ± 0.355

2.557 ± 0.12

4.031 ± 1.161

4.818 ± 0.095

7.965 ± 1.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.275

3.835 ± 0.013

6.883 ± 1.094

4.326 ± 0.203

6.785 ± 0.126

6.981 ± 0.135

5.801 ± 0.979

6.588 ± 0.269

1.475 ± 0.005

3.54 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski