Pedobacter sp. HMWF019

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; unclassified Pedobacter

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5695 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R7KPD6|A0A2R7KPD6_9SPHI Uncharacterized protein OS=Pedobacter sp. HMWF019 OX=2056856 GN=DBR11_12680 PE=4 SV=1
MM1 pKa = 7.89AILKK5 pKa = 10.34LEE7 pKa = 4.09YY8 pKa = 10.34QQDD11 pKa = 4.07DD12 pKa = 4.32YY13 pKa = 12.09EE14 pKa = 4.49SLQEE18 pKa = 4.39GEE20 pKa = 4.53LSAKK24 pKa = 10.11EE25 pKa = 3.96FLNEE29 pKa = 3.81VLFSGSYY36 pKa = 10.66EE37 pKa = 4.51DD38 pKa = 5.05ISPEE42 pKa = 4.38DD43 pKa = 3.68EE44 pKa = 4.92VIDD47 pKa = 4.37ILLGYY52 pKa = 9.26HH53 pKa = 6.64GSDD56 pKa = 2.96GTIPEE61 pKa = 4.12ISDD64 pKa = 3.93LEE66 pKa = 4.27LEE68 pKa = 4.16EE69 pKa = 5.92AEE71 pKa = 4.36YY72 pKa = 11.21EE73 pKa = 4.15EE74 pKa = 6.17DD75 pKa = 5.16DD76 pKa = 3.83EE77 pKa = 4.61TGKK80 pKa = 10.66ARR82 pKa = 11.84LSYY85 pKa = 10.94LLAYY89 pKa = 10.47SSACAGSNSEE99 pKa = 4.15EE100 pKa = 4.51LISEE104 pKa = 4.39LVNFKK109 pKa = 10.02IDD111 pKa = 3.57QEE113 pKa = 4.33SSKK116 pKa = 10.96VVFTFIDD123 pKa = 3.89LNTRR127 pKa = 11.84STVDD131 pKa = 3.13EE132 pKa = 4.62FF133 pKa = 4.77

Molecular weight: 14.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R7L094|A0A2R7L094_9SPHI DUF3108 domain-containing protein OS=Pedobacter sp. HMWF019 OX=2056856 GN=DBR11_03250 PE=4 SV=1
MM1 pKa = 7.15IQRR4 pKa = 11.84IQSVWLLLTSLTLFILLALPVVSKK28 pKa = 9.87TNNGTEE34 pKa = 4.28YY35 pKa = 10.19IVYY38 pKa = 9.84TNGLHH43 pKa = 5.89QIKK46 pKa = 10.1NVSSEE51 pKa = 3.83THH53 pKa = 5.16IRR55 pKa = 11.84ADD57 pKa = 2.93VFMPLMVSNIIIAILAFIAIFLFRR81 pKa = 11.84NRR83 pKa = 11.84TLQKK87 pKa = 9.99RR88 pKa = 11.84ICSLIIILIAAFNFWVFQSARR109 pKa = 11.84EE110 pKa = 4.06IPGGLDD116 pKa = 3.12GASFGAGAFLPLVAIAFCFLAIRR139 pKa = 11.84GIRR142 pKa = 11.84KK143 pKa = 9.58DD144 pKa = 3.43EE145 pKa = 3.84QLIRR149 pKa = 11.84SADD152 pKa = 3.76RR153 pKa = 11.84LRR155 pKa = 5.24

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5695

0

5695

2068325

12

3078

363.2

40.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.158 ± 0.03

0.728 ± 0.01

5.2 ± 0.019

5.572 ± 0.034

4.895 ± 0.02

6.932 ± 0.035

1.768 ± 0.016

7.079 ± 0.027

7.211 ± 0.025

9.728 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.225 ± 0.014

5.761 ± 0.031

3.807 ± 0.017

3.843 ± 0.019

3.863 ± 0.018

6.718 ± 0.028

5.756 ± 0.034

6.231 ± 0.025

1.207 ± 0.012

4.316 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski