Dinothrombium tinctorium
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13518 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S3P0X2|A0A3S3P0X2_9ACAR Protein lin-9-like protein OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_00973 PE=3 SV=1
MM1 pKa = 7.35 ATMDD5 pKa = 4.44 SSGGDD10 pKa = 3.12 NDD12 pKa = 3.43 VSPFLDD18 pKa = 4.41 EE19 pKa = 4.23 ILLNKK24 pKa = 10.13 SVTIRR29 pKa = 11.84 PDD31 pKa = 3.3 DD32 pKa = 3.93 VNATNDD38 pKa = 3.7 YY39 pKa = 10.51 NWIFLFLIAFIFIGIVGNVLVCLAICLEE67 pKa = 4.0 RR68 pKa = 11.84 RR69 pKa = 11.84 LQNATNYY76 pKa = 9.84 FLLSLAVADD85 pKa = 4.48 LLVCVVVMPILILDD99 pKa = 3.98 DD100 pKa = 5.47 FYY102 pKa = 11.93 ACKK105 pKa = 10.42
Molecular weight: 11.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.846
Sillero 3.948
Patrickios 0.782
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|A0A3S3NPG7|A0A3S3NPG7_9ACAR Tetraspanin OS=Dinothrombium tinctorium OX=1965070 GN=B4U79_05050 PE=3 SV=1
II1 pKa = 7.0 SARR4 pKa = 11.84 TRR6 pKa = 11.84 ISARR10 pKa = 11.84 TSISTKK16 pKa = 8.45 TRR18 pKa = 11.84 ISSIARR24 pKa = 11.84 ISTRR28 pKa = 11.84 TRR30 pKa = 11.84 IFYY33 pKa = 7.74 RR34 pKa = 11.84 TRR36 pKa = 11.84 ISSRR40 pKa = 11.84 TRR42 pKa = 11.84 ISARR46 pKa = 11.84 TRR48 pKa = 11.84 ISARR52 pKa = 11.84 TNISTKK58 pKa = 8.57 TRR60 pKa = 11.84 ISSIARR66 pKa = 11.84 ISTRR70 pKa = 11.84 TRR72 pKa = 11.84 IFYY75 pKa = 7.75 RR76 pKa = 11.84 TRR78 pKa = 11.84 ILYY81 pKa = 9.97 RR82 pKa = 11.84 SRR84 pKa = 11.84 SSSRR88 pKa = 11.84 NRR90 pKa = 11.84 ISTSSRR96 pKa = 11.84 ISIRR100 pKa = 11.84 FRR102 pKa = 11.84 IFYY105 pKa = 9.89 RR106 pKa = 11.84 SPNSIVII113 pKa = 3.83
Molecular weight: 13.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.422
IPC2_protein 11.242
IPC_protein 12.515
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.208
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.915
IPC_peptide 13.173
IPC2_peptide 12.149
IPC2.peptide.svr19 9.113
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13518
0
13518
5891483
8
5318
435.8
49.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.755 ± 0.017
2.15 ± 0.016
5.223 ± 0.014
6.791 ± 0.024
4.776 ± 0.019
4.877 ± 0.021
2.299 ± 0.01
6.7 ± 0.019
7.164 ± 0.021
9.269 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.079 ± 0.007
5.796 ± 0.017
4.285 ± 0.022
3.839 ± 0.016
4.87 ± 0.015
8.394 ± 0.027
5.312 ± 0.015
5.944 ± 0.017
1.094 ± 0.006
3.367 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here