Staphylococcus phage StauST398-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Biseptimavirus; Staphylococcus virus StauST398-4

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5R9G5|W5R9G5_9CAUD Terminase large subunit OS=Staphylococcus phage StauST398-4 OX=1308896 GN=StauST398-4_0039 PE=4 SV=1
MM1 pKa = 7.41YY2 pKa = 10.23EE3 pKa = 3.56LGKK6 pKa = 10.79YY7 pKa = 7.28VTEE10 pKa = 3.84QVYY13 pKa = 10.94IMMTANDD20 pKa = 4.14DD21 pKa = 3.7VEE23 pKa = 5.3APSDD27 pKa = 3.63YY28 pKa = 11.0VFRR31 pKa = 11.84AEE33 pKa = 4.19VSEE36 pKa = 4.17

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5R9H0|W5R9H0_9CAUD Uncharacterized protein OS=Staphylococcus phage StauST398-4 OX=1308896 GN=StauST398-4_0044 PE=4 SV=1
MM1 pKa = 7.43YY2 pKa = 10.54ILMNRR7 pKa = 11.84KK8 pKa = 8.92SRR10 pKa = 11.84IVSNLLKK17 pKa = 10.9NGIFQHH23 pKa = 5.1YY24 pKa = 8.73HH25 pKa = 5.54FKK27 pKa = 11.01GLSS30 pKa = 3.11

Molecular weight: 3.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12463

30

1509

191.7

21.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.649 ± 0.406

0.514 ± 0.105

6.098 ± 0.348

7.743 ± 0.517

4.132 ± 0.291

5.753 ± 0.678

1.829 ± 0.155

7.815 ± 0.291

9.693 ± 0.386

8.056 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.19

7.101 ± 0.337

2.616 ± 0.154

3.964 ± 0.3

4.237 ± 0.284

5.857 ± 0.248

5.504 ± 0.215

5.753 ± 0.229

0.979 ± 0.143

4.237 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski