Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillu

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179BMN5|A0A179BMN5_ACIFR Uncharacterized protein OS=Acidithiobacillus ferrooxidans OX=920 GN=A4H96_03590 PE=4 SV=1
MM1 pKa = 7.0ATGYY5 pKa = 10.96VDD7 pKa = 3.42GFEE10 pKa = 4.17RR11 pKa = 11.84AMGVLGLDD19 pKa = 3.58VEE21 pKa = 5.65AMEE24 pKa = 5.18EE25 pKa = 3.9WLLDD29 pKa = 3.82HH30 pKa = 7.01NIEE33 pKa = 4.63RR34 pKa = 11.84CPSCDD39 pKa = 2.61WFVASHH45 pKa = 6.43EE46 pKa = 4.18LLNDD50 pKa = 3.22EE51 pKa = 4.99GEE53 pKa = 4.31IDD55 pKa = 4.39GLCDD59 pKa = 3.21NCRR62 pKa = 11.84SS63 pKa = 3.59

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A179B793|A0A179B793_ACIFR Uncharacterized protein OS=Acidithiobacillus ferrooxidans OX=920 GN=A4H96_13160 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVVHH11 pKa = 6.89RR12 pKa = 11.84KK13 pKa = 7.38RR14 pKa = 11.84THH16 pKa = 5.56GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29SGRR28 pKa = 11.84LVLKK32 pKa = 10.26RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.25GRR39 pKa = 11.84QRR41 pKa = 11.84LCPP44 pKa = 3.78

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2816

0

2816

808942

30

2856

287.3

31.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.064 ± 0.066

0.972 ± 0.017

5.134 ± 0.037

5.51 ± 0.045

3.561 ± 0.034

7.915 ± 0.045

2.683 ± 0.025

5.456 ± 0.039

3.351 ± 0.038

10.892 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.021

2.953 ± 0.037

5.074 ± 0.033

4.299 ± 0.029

6.799 ± 0.053

5.467 ± 0.037

5.061 ± 0.031

6.992 ± 0.041

1.595 ± 0.023

2.621 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski