Beihai weivirus-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KL33|A0A1L3KL33_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 12 OX=1922741 PE=4 SV=1
MM1 pKa = 7.39AVVSEE6 pKa = 4.48RR7 pKa = 11.84PSVSSVPLCPTCHH20 pKa = 6.17RR21 pKa = 11.84QHH23 pKa = 7.02LDD25 pKa = 3.16KK26 pKa = 11.15LSEE29 pKa = 3.69EE30 pKa = 4.43AAWDD34 pKa = 3.74RR35 pKa = 11.84ASKK38 pKa = 10.85DD39 pKa = 3.46EE40 pKa = 4.16SHH42 pKa = 5.87VVCVKK47 pKa = 8.82FHH49 pKa = 4.87VRR51 pKa = 11.84KK52 pKa = 9.64CAFCTDD58 pKa = 2.66VDD60 pKa = 4.03INCRR64 pKa = 11.84RR65 pKa = 11.84SFFSRR70 pKa = 11.84FTPRR74 pKa = 11.84TAHH77 pKa = 6.41SGDD80 pKa = 3.65SVLCEE85 pKa = 3.54VHH87 pKa = 5.62MTHH90 pKa = 6.47QEE92 pKa = 3.53LRR94 pKa = 11.84IARR97 pKa = 11.84DD98 pKa = 3.24AVGASLYY105 pKa = 10.77SEE107 pKa = 4.35APSRR111 pKa = 11.84VGNSNLHH118 pKa = 5.5LSGMRR123 pKa = 11.84LDD125 pKa = 4.78RR126 pKa = 11.84KK127 pKa = 10.66YY128 pKa = 11.25EE129 pKa = 3.89DD130 pKa = 4.21TEE132 pKa = 4.5MVWARR137 pKa = 11.84DD138 pKa = 3.6VVFPAVAIAMHH149 pKa = 7.25RR150 pKa = 11.84SRR152 pKa = 11.84EE153 pKa = 4.17TPVFSLGASIFPRR166 pKa = 11.84LRR168 pKa = 11.84KK169 pKa = 9.41LRR171 pKa = 11.84LEE173 pKa = 4.29SEE175 pKa = 4.44TGSKK179 pKa = 9.28RR180 pKa = 11.84WFWIRR185 pKa = 11.84ASQHH189 pKa = 4.48MTLEE193 pKa = 4.3CLRR196 pKa = 11.84FCPRR200 pKa = 11.84RR201 pKa = 11.84VEE203 pKa = 4.06QPTEE207 pKa = 3.66EE208 pKa = 4.61VYY210 pKa = 11.19APVATITPDD219 pKa = 3.07PGYY222 pKa = 9.12TFTRR226 pKa = 11.84FDD228 pKa = 3.64GVDD231 pKa = 3.25RR232 pKa = 11.84AATRR236 pKa = 11.84QRR238 pKa = 11.84EE239 pKa = 4.46GWDD242 pKa = 3.23LMASKK247 pKa = 10.93VFGVDD252 pKa = 4.08LMTDD256 pKa = 3.14AAGNPTLGDD265 pKa = 3.55NILAVRR271 pKa = 11.84HH272 pKa = 5.9LPFFARR278 pKa = 11.84KK279 pKa = 9.28IFFDD283 pKa = 3.48TLPRR287 pKa = 11.84NIAAAIAGRR296 pKa = 11.84IEE298 pKa = 4.5NVPPMQMTDD307 pKa = 3.5DD308 pKa = 3.9EE309 pKa = 4.42QAEE312 pKa = 4.2LRR314 pKa = 11.84GVTEE318 pKa = 4.42ALSICLRR325 pKa = 11.84RR326 pKa = 11.84DD327 pKa = 2.84AKK329 pKa = 9.86MIRR332 pKa = 11.84EE333 pKa = 4.03IVTVLTLGDD342 pKa = 3.6WKK344 pKa = 10.76SKK346 pKa = 9.39KK347 pKa = 6.62WTITRR352 pKa = 11.84SKK354 pKa = 7.75TTLEE358 pKa = 4.03QLRR361 pKa = 11.84QRR363 pKa = 11.84YY364 pKa = 8.55NPTYY368 pKa = 9.93QFKK371 pKa = 10.6GQIKK375 pKa = 10.4LEE377 pKa = 4.01PSKK380 pKa = 10.52PGKK383 pKa = 9.38PPRR386 pKa = 11.84LIIADD391 pKa = 3.59GDD393 pKa = 4.01YY394 pKa = 10.12GQVMAWTIIATLEE407 pKa = 3.43RR408 pKa = 11.84WIFKK412 pKa = 10.09RR413 pKa = 11.84YY414 pKa = 6.07NHH416 pKa = 6.68RR417 pKa = 11.84SIKK420 pKa = 10.18GVCKK424 pKa = 10.03SDD426 pKa = 2.75AMKK429 pKa = 10.78RR430 pKa = 11.84LIKK433 pKa = 9.87NTAQFRR439 pKa = 11.84PSTGRR444 pKa = 11.84STTRR448 pKa = 11.84EE449 pKa = 3.56PVMVLEE455 pKa = 4.82NDD457 pKa = 3.32GSAWDD462 pKa = 3.61ACMSVALRR470 pKa = 11.84ALTEE474 pKa = 3.97NVLMQEE480 pKa = 4.06VHH482 pKa = 7.64DD483 pKa = 3.83ITKK486 pKa = 10.53DD487 pKa = 3.47LFIMEE492 pKa = 4.6SHH494 pKa = 6.3WQPEE498 pKa = 3.92RR499 pKa = 11.84LRR501 pKa = 11.84ANKK504 pKa = 9.66VKK506 pKa = 9.68TLSLSVAAKK515 pKa = 8.41FNCYY519 pKa = 9.85DD520 pKa = 3.26EE521 pKa = 5.62HH522 pKa = 9.08VPDD525 pKa = 4.76DD526 pKa = 3.86VRR528 pKa = 11.84NALAAGKK535 pKa = 8.14TYY537 pKa = 10.84KK538 pKa = 10.44EE539 pKa = 4.01VIAAIRR545 pKa = 11.84RR546 pKa = 11.84SGCRR550 pKa = 11.84GTSVLNFLANMILWCWVLGGKK571 pKa = 9.8DD572 pKa = 3.71GSRR575 pKa = 11.84LMEE578 pKa = 4.46SNGQKK583 pKa = 9.18FTDD586 pKa = 3.36VFGVVRR592 pKa = 11.84FVRR595 pKa = 11.84MMFEE599 pKa = 4.73GDD601 pKa = 4.07DD602 pKa = 3.85SLLTLTGARR611 pKa = 11.84FTPEE615 pKa = 3.27QVAQLSARR623 pKa = 11.84WTKK626 pKa = 10.48LGHH629 pKa = 5.76RR630 pKa = 11.84PKK632 pKa = 10.79LFVRR636 pKa = 11.84APGDD640 pKa = 3.3QAEE643 pKa = 4.24FTGYY647 pKa = 10.67KK648 pKa = 10.11FLVDD652 pKa = 3.37EE653 pKa = 5.38HH654 pKa = 8.01GLVPDD659 pKa = 4.44SEE661 pKa = 5.07VPDD664 pKa = 4.03LPRR667 pKa = 11.84LLGNVSYY674 pKa = 10.29CHH676 pKa = 6.38NRR678 pKa = 11.84GAVDD682 pKa = 3.38AAVKK686 pKa = 10.4GDD688 pKa = 3.44EE689 pKa = 4.36AALHH693 pKa = 5.98RR694 pKa = 11.84AVDD697 pKa = 3.8PGLLSRR703 pKa = 11.84AYY705 pKa = 9.27TIARR709 pKa = 11.84KK710 pKa = 9.85APTVARR716 pKa = 11.84WLYY719 pKa = 9.9NQVKK723 pKa = 8.57STDD726 pKa = 3.56LTFSNDD732 pKa = 2.86DD733 pKa = 3.51RR734 pKa = 11.84VRR736 pKa = 11.84LDD738 pKa = 4.42DD739 pKa = 5.23DD740 pKa = 4.76LGDD743 pKa = 4.0VLPEE747 pKa = 4.04LWKK750 pKa = 9.79GTDD753 pKa = 3.33GTEE756 pKa = 4.24KK757 pKa = 10.15MLEE760 pKa = 4.07QAISWQTFVEE770 pKa = 4.42RR771 pKa = 11.84VEE773 pKa = 4.44SEE775 pKa = 3.66ISAGEE780 pKa = 3.6AAGFDD785 pKa = 3.82EE786 pKa = 6.17AGFAYY791 pKa = 10.18AHH793 pKa = 6.67GWCTSTEE800 pKa = 3.68QWNDD804 pKa = 2.94FLIALGAITRR814 pKa = 11.84GTDD817 pKa = 3.22SVVVGRR823 pKa = 11.84VLPSCFF829 pKa = 3.47

Molecular weight: 93.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KL33|A0A1L3KL33_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 12 OX=1922741 PE=4 SV=1
MM1 pKa = 7.66PASKK5 pKa = 9.87SQRR8 pKa = 11.84VRR10 pKa = 11.84AVMKK14 pKa = 10.44KK15 pKa = 10.24ALKK18 pKa = 10.07KK19 pKa = 10.27KK20 pKa = 10.24QGGANLYY27 pKa = 10.18GIKK30 pKa = 10.01QGAAMVNTRR39 pKa = 11.84PFPRR43 pKa = 11.84RR44 pKa = 11.84ARR46 pKa = 11.84RR47 pKa = 11.84NGRR50 pKa = 11.84GSSDD54 pKa = 2.97ARR56 pKa = 11.84LATMIKK62 pKa = 9.74YY63 pKa = 9.81GLNALHH69 pKa = 7.11PCHH72 pKa = 7.02LALPRR77 pKa = 11.84AVGGYY82 pKa = 9.13SVVRR86 pKa = 11.84TTDD89 pKa = 3.48IINSNAATMFFGTFKK104 pKa = 11.21GPGNQFTEE112 pKa = 4.77TTWLSTIAIRR122 pKa = 11.84GVAPDD127 pKa = 3.49TAINGTGNAFLYY139 pKa = 10.73NSSALQAPSLSGTRR153 pKa = 11.84MVPAAMTVQIMNGQSLQNADD173 pKa = 3.71GIAYY177 pKa = 9.27IGKK180 pKa = 9.69SKK182 pKa = 9.6TVLDD186 pKa = 4.01LMGDD190 pKa = 3.35TRR192 pKa = 11.84TWRR195 pKa = 11.84TVFEE199 pKa = 4.7DD200 pKa = 3.83LVSYY204 pKa = 9.24SAPRR208 pKa = 11.84LCSGGKK214 pKa = 8.87LALRR218 pKa = 11.84GVQVDD223 pKa = 3.9AVPNNLSVLSDD234 pKa = 3.65FVPRR238 pKa = 11.84RR239 pKa = 11.84IPTEE243 pKa = 4.05GARR246 pKa = 11.84TWDD249 pKa = 3.66EE250 pKa = 3.91DD251 pKa = 3.44QAALDD256 pKa = 4.09FEE258 pKa = 4.81GFAPIFVYY266 pKa = 10.8NPDD269 pKa = 4.03NIDD272 pKa = 3.57LKK274 pKa = 11.22FLVTIEE280 pKa = 3.09WRR282 pKa = 11.84MRR284 pKa = 11.84FDD286 pKa = 4.19PLNPAYY292 pKa = 10.0AGHH295 pKa = 5.73VTHH298 pKa = 7.08TPAAEE303 pKa = 4.33STWSKK308 pKa = 10.97VLAHH312 pKa = 6.74AEE314 pKa = 4.23SLGHH318 pKa = 5.95GVMDD322 pKa = 3.97IADD325 pKa = 3.58VVADD329 pKa = 3.92VGAAGLRR336 pKa = 11.84ARR338 pKa = 11.84DD339 pKa = 3.92AIAPLLAA346 pKa = 5.3

Molecular weight: 37.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1175

346

829

587.5

65.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.128 ± 1.59

1.702 ± 0.621

6.213 ± 0.399

4.851 ± 1.403

4.255 ± 0.275

6.809 ± 1.029

2.213 ± 0.264

4.17 ± 0.251

4.511 ± 0.257

8.426 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.205

3.404 ± 0.834

4.766 ± 0.401

2.809 ± 0.045

8.17 ± 0.682

6.298 ± 0.126

6.723 ± 0.118

7.745 ± 0.287

2.043 ± 0.33

1.957 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski