Klebsiella phage vB_KpnP_KpV763
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8F0E1|A0A1D8F0E1_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnP_KpV763 OX=1882400 GN=kpv763_20 PE=4 SV=1
MM1 pKa = 6.63 VTQALIIFGLPILIVGTLYY20 pKa = 10.93 CINKK24 pKa = 8.77 EE25 pKa = 4.07 WFSDD29 pKa = 3.72 GQEE32 pKa = 3.75
Molecular weight: 3.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.062
IPC2_protein 4.546
IPC_protein 3.961
Toseland 3.859
ProMoST 3.973
Dawson 3.948
Bjellqvist 4.431
Wikipedia 3.923
Rodwell 3.846
Grimsley 3.808
Solomon 3.872
Lehninger 3.834
Nozaki 4.151
DTASelect 4.151
Thurlkill 3.961
EMBOSS 3.923
Sillero 4.088
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 4.062
IPC2.peptide.svr19 4.26
Protein with the highest isoelectric point:
>tr|A0A1D8F0I3|A0A1D8F0I3_9CAUD Putative primase/helicase protein OS=Klebsiella phage vB_KpnP_KpV763 OX=1882400 GN=kpv763_21 PE=4 SV=1
MM1 pKa = 7.88 RR2 pKa = 11.84 LHH4 pKa = 6.7 FNKK7 pKa = 10.46 SNGIFSVRR15 pKa = 11.84 RR16 pKa = 11.84 EE17 pKa = 4.04 DD18 pKa = 3.6 RR19 pKa = 11.84 STVAATEE26 pKa = 3.98 RR27 pKa = 11.84 HH28 pKa = 5.03 GKK30 pKa = 8.71 IPRR33 pKa = 11.84 IGDD36 pKa = 3.66 TFEE39 pKa = 4.6 LAPSVHH45 pKa = 7.02 ILITRR50 pKa = 11.84 GLYY53 pKa = 9.91 EE54 pKa = 4.18 LAQTKK59 pKa = 9.86 SRR61 pKa = 11.84 PFVPVVVTKK70 pKa = 9.98 WPRR73 pKa = 11.84 LRR75 pKa = 11.84 LFWEE79 pKa = 4.92 RR80 pKa = 11.84 IKK82 pKa = 11.03 EE83 pKa = 4.19 AVNDD87 pKa = 3.95
Molecular weight: 10.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.663
IPC_protein 10.687
Toseland 11.038
ProMoST 11.023
Dawson 11.067
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.169
Grimsley 11.096
Solomon 11.33
Lehninger 11.286
Nozaki 11.008
DTASelect 10.862
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.023
Patrickios 10.935
IPC_peptide 11.345
IPC2_peptide 9.882
IPC2.peptide.svr19 8.922
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
49
0
49
12443
32
1321
253.9
28.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.929 ± 0.51
1.045 ± 0.199
6.285 ± 0.207
6.944 ± 0.413
3.649 ± 0.173
8.077 ± 0.394
1.752 ± 0.223
4.951 ± 0.196
6.526 ± 0.428
8.246 ± 0.358
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.644 ± 0.162
4.316 ± 0.32
3.673 ± 0.171
4.211 ± 0.333
5.441 ± 0.25
6.003 ± 0.32
5.682 ± 0.307
6.831 ± 0.397
1.414 ± 0.182
3.383 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here