Pseudomonas phage phiYY

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus; Pseudomonas virus phiYY

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W2KDS0|A0A1W2KDS0_9VIRU Putative procapsid protein OS=Pseudomonas phage phiYY OX=1852644 GN=phiYY_sL2 PE=4 SV=1
MM1 pKa = 8.37DD2 pKa = 5.1YY3 pKa = 11.48GPMVAGTDD11 pKa = 3.56PSVPLAPTLGAFLRR25 pKa = 11.84NSDD28 pKa = 3.85VEE30 pKa = 4.48KK31 pKa = 9.76VPRR34 pKa = 11.84WAEE37 pKa = 3.86VIGASGFTDD46 pKa = 3.72EE47 pKa = 4.69SNMYY51 pKa = 9.96EE52 pKa = 4.54VIFHH56 pKa = 6.79PNVIVRR62 pKa = 11.84IEE64 pKa = 3.97LMQAASAFIEE74 pKa = 4.24AAKK77 pKa = 10.82NGAA80 pKa = 3.63

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W2KDR8|A0A1W2KDR8_9VIRU Uncharacterized protein OS=Pseudomonas phage phiYY OX=1852644 GN=phiYY_sS1 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84SASSNEE8 pKa = 3.84LTHH11 pKa = 5.49MTVAQLPYY19 pKa = 9.46RR20 pKa = 11.84HH21 pKa = 6.58SKK23 pKa = 10.25NSSDD27 pKa = 3.39SRR29 pKa = 11.84QIRR32 pKa = 11.84YY33 pKa = 9.77GGTSRR38 pKa = 11.84SPRR41 pKa = 11.84QILSTDD47 pKa = 3.19TRR49 pKa = 11.84PLNEE53 pKa = 3.69GAKK56 pKa = 9.93PYY58 pKa = 10.87NCFISSPYY66 pKa = 10.51ANRR69 pKa = 11.84ASALRR74 pKa = 11.84MM75 pKa = 3.69

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18

0

18

4273

45

805

237.4

25.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.169 ± 0.637

0.842 ± 0.187

4.751 ± 0.47

5.593 ± 0.457

3.37 ± 0.344

7.372 ± 0.417

1.849 ± 0.179

4.564 ± 0.419

4.259 ± 0.549

9.314 ± 0.369

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.206 ± 0.234

3.3 ± 0.344

5.078 ± 0.324

2.832 ± 0.355

5.991 ± 0.473

7.7 ± 0.649

5.734 ± 0.333

7.934 ± 0.691

1.428 ± 0.327

2.715 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski