Nitrincola tapanii
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2528 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5A9VYS8|A0A5A9VYS8_9GAMM Phenol hydroxylase OS=Nitrincola tapanii OX=1708751 GN=E1H14_12755 PE=4 SV=1
MM1 pKa = 7.53 TEE3 pKa = 3.83 QADD6 pKa = 3.79 EE7 pKa = 4.26 MTLHH11 pKa = 6.2 FTQAAAAKK19 pKa = 8.73 VRR21 pKa = 11.84 SLIEE25 pKa = 3.79 EE26 pKa = 4.3 EE27 pKa = 4.56 EE28 pKa = 3.97 NDD30 pKa = 3.67 NLKK33 pKa = 10.76 LRR35 pKa = 11.84 VFITGGGCAGFSYY48 pKa = 10.76 GFTFDD53 pKa = 3.85 EE54 pKa = 5.58 DD55 pKa = 3.76 VAEE58 pKa = 5.44 DD59 pKa = 3.68 DD60 pKa = 4.37 TAITTDD66 pKa = 3.6 GVTLLVDD73 pKa = 3.75 PMSFQYY79 pKa = 11.0 LVGAQVDD86 pKa = 4.26 YY87 pKa = 11.6 QEE89 pKa = 5.03 GLHH92 pKa = 6.53 GSQFMIKK99 pKa = 9.85 NPNAATTCGCGSSFSII115 pKa = 5.02
Molecular weight: 12.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.796
IPC2_protein 4.012
IPC_protein 3.935
Toseland 3.745
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.846
Nozaki 4.037
DTASelect 4.215
Thurlkill 3.795
EMBOSS 3.834
Sillero 4.05
Patrickios 1.914
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.94
Protein with the highest isoelectric point:
>tr|A0A5A9W0X3|A0A5A9W0X3_9GAMM Uncharacterized protein OS=Nitrincola tapanii OX=1708751 GN=E1H14_10955 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.45 NGRR28 pKa = 11.84 QVLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.89 SLTVV44 pKa = 3.12
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2528
0
2528
850769
23
1600
336.5
37.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.913 ± 0.055
1.04 ± 0.018
5.129 ± 0.039
6.575 ± 0.044
3.75 ± 0.031
6.88 ± 0.05
2.381 ± 0.022
5.413 ± 0.042
3.577 ± 0.039
12.227 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.458 ± 0.023
3.029 ± 0.028
4.543 ± 0.035
5.613 ± 0.052
6.179 ± 0.038
6.032 ± 0.034
4.744 ± 0.027
6.529 ± 0.044
1.391 ± 0.025
2.597 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here