Zinderia insecticola (strain CARI)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Candidatus Zinderia; Candidatus Zinderia insecticola

Average proteome isoelectric point is 9.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0TIY9|E0TIY9_ZINIC Protein GrpE OS=Zinderia insecticola (strain CARI) OX=871271 GN=grpE PE=3 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.24IIGIDD8 pKa = 3.1LGTTNSCVSIMEE20 pKa = 4.42GNQSKK25 pKa = 10.82VIEE28 pKa = 4.24NSEE31 pKa = 4.17GSRR34 pKa = 11.84TTPSVVAYY42 pKa = 9.35QEE44 pKa = 3.85NGEE47 pKa = 4.28ILVGSPAKK55 pKa = 10.2RR56 pKa = 11.84QAITNPKK63 pKa = 7.29NTIYY67 pKa = 10.56AAKK70 pKa = 10.01RR71 pKa = 11.84LIGRR75 pKa = 11.84KK76 pKa = 8.68FNEE79 pKa = 4.23KK80 pKa = 9.68EE81 pKa = 3.97VQKK84 pKa = 10.87DD85 pKa = 3.18IDD87 pKa = 3.57IMPYY91 pKa = 10.41EE92 pKa = 4.61IIKK95 pKa = 10.61SNNGDD100 pKa = 2.57AWINIRR106 pKa = 11.84NKK108 pKa = 10.26KK109 pKa = 8.65IAPPQISAEE118 pKa = 4.18VLKK121 pKa = 10.99KK122 pKa = 9.74MKK124 pKa = 9.1KK125 pKa = 7.18TAEE128 pKa = 4.41DD129 pKa = 3.66YY130 pKa = 11.45LGFKK134 pKa = 10.41VNEE137 pKa = 4.05AVITVPAYY145 pKa = 10.64FNDD148 pKa = 3.82AQRR151 pKa = 11.84QATKK155 pKa = 10.58DD156 pKa = 3.07AGRR159 pKa = 11.84IAGLEE164 pKa = 3.98VKK166 pKa = 10.42RR167 pKa = 11.84IINEE171 pKa = 3.62PTAAALAFGLDD182 pKa = 3.21KK183 pKa = 11.23VLNNDD188 pKa = 3.38KK189 pKa = 10.79KK190 pKa = 10.67IAVYY194 pKa = 10.58DD195 pKa = 3.97LGGGTFDD202 pKa = 3.69ISIIEE207 pKa = 3.79ISNIEE212 pKa = 4.02GEE214 pKa = 4.33KK215 pKa = 10.16QFEE218 pKa = 4.28VLSTNGDD225 pKa = 3.61TFLGGEE231 pKa = 4.52DD232 pKa = 4.01FDD234 pKa = 5.48KK235 pKa = 11.37KK236 pKa = 11.08LIDD239 pKa = 3.59YY240 pKa = 8.47VINEE244 pKa = 4.15FKK246 pKa = 10.84KK247 pKa = 10.74NNGIDD252 pKa = 3.64LYY254 pKa = 10.58KK255 pKa = 10.94DD256 pKa = 3.54SIALQRR262 pKa = 11.84IKK264 pKa = 11.08SSVEE268 pKa = 3.37RR269 pKa = 11.84AKK271 pKa = 10.58IEE273 pKa = 3.98LSSLYY278 pKa = 8.58EE279 pKa = 3.94TEE281 pKa = 3.87INEE284 pKa = 4.72PYY286 pKa = 9.25ITVNNNIPLHH296 pKa = 6.41LNLKK300 pKa = 7.58ITRR303 pKa = 11.84SKK305 pKa = 11.0FEE307 pKa = 4.34SLVEE311 pKa = 3.85DD312 pKa = 5.66LINKK316 pKa = 7.35TIKK319 pKa = 9.77PCEE322 pKa = 3.97IAVKK326 pKa = 10.01DD327 pKa = 3.59AKK329 pKa = 10.89ININEE334 pKa = 3.97ISDD337 pKa = 4.06VILVGGMTRR346 pKa = 11.84MPKK349 pKa = 9.5IQEE352 pKa = 3.81KK353 pKa = 10.83VKK355 pKa = 10.6IFFNKK360 pKa = 9.8EE361 pKa = 3.34PRR363 pKa = 11.84KK364 pKa = 10.42DD365 pKa = 3.57INPDD369 pKa = 2.89EE370 pKa = 4.35AVAIGAAIQGAVISGDD386 pKa = 3.66KK387 pKa = 10.45KK388 pKa = 11.05DD389 pKa = 5.02LLLLDD394 pKa = 3.97VTPLSLGIEE403 pKa = 4.29TMGGIMTKK411 pKa = 9.83MIKK414 pKa = 10.32KK415 pKa = 8.22NTTIPTKK422 pKa = 10.43FSQVFSTAEE431 pKa = 3.91DD432 pKa = 3.65NQPMVTIRR440 pKa = 11.84IYY442 pKa = 10.02QGEE445 pKa = 4.08RR446 pKa = 11.84EE447 pKa = 4.02MANNNKK453 pKa = 9.84LLGEE457 pKa = 4.27FNLEE461 pKa = 4.54GIEE464 pKa = 4.16SAPRR468 pKa = 11.84GVPQIEE474 pKa = 4.17VTFDD478 pKa = 2.89IDD480 pKa = 3.83VNGILHH486 pKa = 5.51VTAKK490 pKa = 10.65DD491 pKa = 3.26KK492 pKa = 10.17NTNKK496 pKa = 9.92EE497 pKa = 3.71NKK499 pKa = 8.35ITIKK503 pKa = 10.79ANSGLNEE510 pKa = 3.9NEE512 pKa = 3.66IEE514 pKa = 4.89KK515 pKa = 9.79MIKK518 pKa = 9.97DD519 pKa = 3.51AEE521 pKa = 4.38INSEE525 pKa = 4.05EE526 pKa = 4.12DD527 pKa = 3.43KK528 pKa = 10.96KK529 pKa = 11.17IKK531 pKa = 10.45DD532 pKa = 3.88LIEE535 pKa = 4.24VKK537 pKa = 10.78NKK539 pKa = 10.35ADD541 pKa = 3.9TLIHH545 pKa = 6.02SANKK549 pKa = 9.9IIEE552 pKa = 4.37KK553 pKa = 10.03NSNLISEE560 pKa = 4.26NDD562 pKa = 3.11INNLKK567 pKa = 10.59KK568 pKa = 10.62EE569 pKa = 4.13IIEE572 pKa = 4.59LEE574 pKa = 4.19DD575 pKa = 3.95LVKK578 pKa = 11.17LNDD581 pKa = 4.15KK582 pKa = 10.7IKK584 pKa = 10.49IEE586 pKa = 4.01NKK588 pKa = 9.48INSLSVILQKK598 pKa = 10.56INSEE602 pKa = 3.76IYY604 pKa = 10.06KK605 pKa = 10.42NKK607 pKa = 10.43NINKK611 pKa = 9.34KK612 pKa = 10.75DD613 pKa = 3.47EE614 pKa = 4.87DD615 pKa = 3.55IKK617 pKa = 11.68NNNEE621 pKa = 3.9DD622 pKa = 4.47LEE624 pKa = 4.92KK625 pKa = 10.83NDD627 pKa = 3.44EE628 pKa = 4.24NKK630 pKa = 9.86VVDD633 pKa = 5.23KK634 pKa = 10.95DD635 pKa = 4.04FKK637 pKa = 11.02DD638 pKa = 3.59IKK640 pKa = 10.94

Molecular weight: 71.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0TJ45|E0TJ45_ZINIC 50S ribosomal protein L4 OS=Zinderia insecticola (strain CARI) OX=871271 GN=rplD PE=3 SV=1
MM1 pKa = 7.75IIYY4 pKa = 9.64KK5 pKa = 9.54PKK7 pKa = 7.95PTSPGRR13 pKa = 11.84RR14 pKa = 11.84NVIKK18 pKa = 10.48ILNKK22 pKa = 9.69NLYY25 pKa = 9.13KK26 pKa = 10.66GRR28 pKa = 11.84PLSILLKK35 pKa = 10.2KK36 pKa = 10.09KK37 pKa = 9.9KK38 pKa = 10.02KK39 pKa = 9.88KK40 pKa = 9.96SGRR43 pKa = 11.84NNMGRR48 pKa = 11.84ITTRR52 pKa = 11.84HH53 pKa = 6.2KK54 pKa = 10.47GGQHH58 pKa = 4.35KK59 pKa = 9.92RR60 pKa = 11.84YY61 pKa = 10.13YY62 pKa = 10.45RR63 pKa = 11.84IIDD66 pKa = 3.88FKK68 pKa = 11.13RR69 pKa = 11.84NEE71 pKa = 4.09YY72 pKa = 10.0GIKK75 pKa = 10.4GYY77 pKa = 8.94VKK79 pKa = 10.26RR80 pKa = 11.84IEE82 pKa = 3.97YY83 pKa = 10.47DD84 pKa = 3.15PNRR87 pKa = 11.84NCNIALIYY95 pKa = 9.37YY96 pKa = 10.35LNGKK100 pKa = 8.7KK101 pKa = 10.07KK102 pKa = 10.93YY103 pKa = 9.63ILHH106 pKa = 6.7PKK108 pKa = 8.87NLNINDD114 pKa = 4.36EE115 pKa = 4.52IISNYY120 pKa = 7.41KK121 pKa = 9.2TPIKK125 pKa = 10.23IGNNLLIKK133 pKa = 10.11NIPLGTLIHH142 pKa = 6.44CLEE145 pKa = 4.56LLPFKK150 pKa = 10.45GAQIARR156 pKa = 11.84SAGSSALIISRR167 pKa = 11.84EE168 pKa = 3.78KK169 pKa = 10.65KK170 pKa = 8.08YY171 pKa = 8.72TQVRR175 pKa = 11.84LKK177 pKa = 10.6SNEE180 pKa = 3.02IRR182 pKa = 11.84RR183 pKa = 11.84INNNCFATIGEE194 pKa = 4.4IGNEE198 pKa = 3.59KK199 pKa = 10.58HH200 pKa = 6.83NLIKK204 pKa = 10.6LGKK207 pKa = 10.11AGIKK211 pKa = 9.83RR212 pKa = 11.84NLGIRR217 pKa = 11.84PTVRR221 pKa = 11.84GVAMNPIDD229 pKa = 4.06HH230 pKa = 6.89PHH232 pKa = 6.54GGGEE236 pKa = 4.02GRR238 pKa = 11.84TSGGRR243 pKa = 11.84HH244 pKa = 5.87PVTPWGKK251 pKa = 8.02KK252 pKa = 6.7TKK254 pKa = 10.06GKK256 pKa = 8.43KK257 pKa = 7.59TRR259 pKa = 11.84NKK261 pKa = 10.49KK262 pKa = 10.26KK263 pKa = 9.82ISNNMIINNKK273 pKa = 7.88NDD275 pKa = 3.56KK276 pKa = 10.67IFF278 pKa = 3.77

Molecular weight: 31.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

206

0

206

62563

37

1291

303.7

36.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.646 ± 0.131

1.073 ± 0.051

2.267 ± 0.09

3.277 ± 0.142

6.923 ± 0.251

3.697 ± 0.178

0.964 ± 0.046

17.49 ± 0.258

17.948 ± 0.349

9.296 ± 0.148

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.232 ± 0.062

12.813 ± 0.233

1.966 ± 0.078

1.181 ± 0.061

2.036 ± 0.09

5.185 ± 0.144

2.612 ± 0.111

2.048 ± 0.117

0.599 ± 0.052

5.748 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski