Goose hemorrhagic polyomavirus
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q80FI6|Q80FI6_9POLY ORF-X OS=Goose hemorrhagic polyomavirus OX=208491 GN=ORF-X PE=4 SV=1
MM1 pKa = 7.58 SEE3 pKa = 4.07 ATRR6 pKa = 11.84 KK7 pKa = 9.97 PMDD10 pKa = 3.6 SEE12 pKa = 4.69 GPRR15 pKa = 11.84 AASAAAAPRR24 pKa = 11.84 TEE26 pKa = 4.8 DD27 pKa = 3.91 SEE29 pKa = 4.82 GPPSAAGGSVGDD41 pKa = 4.24 IPAPVPASPAPEE53 pKa = 4.16 EE54 pKa = 4.28 ASGVPPVPEE63 pKa = 3.75 KK64 pKa = 10.88 AGPDD68 pKa = 3.54 GAAAPHH74 pKa = 6.23 ATAGPAGTSSTAAEE88 pKa = 4.33 VTEE91 pKa = 5.12 DD92 pKa = 4.18 PPEE95 pKa = 4.38 STPSPEE101 pKa = 4.06 RR102 pKa = 11.84 DD103 pKa = 3.09 GVSPKK108 pKa = 10.72 EE109 pKa = 4.25 PLTQPSTGSSSTDD122 pKa = 3.21 LSPPDD127 pKa = 4.19 SPHH130 pKa = 7.64 ASDD133 pKa = 6.02 DD134 pKa = 4.4 DD135 pKa = 4.17 MDD137 pKa = 3.91 TSPYY141 pKa = 10.59 YY142 pKa = 10.31 EE143 pKa = 4.81 GSCTCTFSLKK153 pKa = 10.04 VCCNICSGCNEE164 pKa = 4.56 FLNSDD169 pKa = 3.4
Molecular weight: 16.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.956
IPC2_protein 3.948
IPC_protein 3.897
Toseland 3.706
ProMoST 3.999
Dawson 3.872
Bjellqvist 4.062
Wikipedia 3.783
Rodwell 3.732
Grimsley 3.617
Solomon 3.872
Lehninger 3.821
Nozaki 3.999
DTASelect 4.177
Thurlkill 3.745
EMBOSS 3.795
Sillero 4.024
Patrickios 2.778
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.946
Protein with the highest isoelectric point:
>tr|Q80FI5|Q80FI5_9POLY Minor capsid protein OS=Goose hemorrhagic polyomavirus OX=208491 GN=VP2 PE=3 SV=1
MM1 pKa = 7.72 AIVEE5 pKa = 3.99 WRR7 pKa = 11.84 PYY9 pKa = 10.58 VDD11 pKa = 3.42 YY12 pKa = 10.93 LFPGFEE18 pKa = 4.04 TFVNQFYY25 pKa = 10.19 WINPLEE31 pKa = 4.27 WGPTLFQRR39 pKa = 11.84 VGQAFWDD46 pKa = 3.83 LLITEE51 pKa = 4.71 GRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 LGEE58 pKa = 4.0 ATSAAAQTSAQYY70 pKa = 11.06 VYY72 pKa = 11.45 DD73 pKa = 4.9 LIARR77 pKa = 11.84 TAEE80 pKa = 3.98 RR81 pKa = 11.84 ARR83 pKa = 11.84 WTVVEE88 pKa = 4.77 GPGEE92 pKa = 4.14 AYY94 pKa = 10.24 RR95 pKa = 11.84 GLIEE99 pKa = 4.74 YY100 pKa = 10.39 YY101 pKa = 10.99 GEE103 pKa = 4.18 LPPWKK108 pKa = 9.18 PHH110 pKa = 4.93 SLHH113 pKa = 7.13 RR114 pKa = 11.84 GLPWEE119 pKa = 4.26 QKK121 pKa = 5.82 TTEE124 pKa = 4.15 TGGNGQPRR132 pKa = 11.84 QQPDD136 pKa = 3.01 SGEE139 pKa = 4.1 TVYY142 pKa = 10.46 QQPVPGGANQRR153 pKa = 11.84 AAPDD157 pKa = 3.11 WMLPLILGLYY167 pKa = 7.05 GTVYY171 pKa = 10.26 PGWKK175 pKa = 10.19 AEE177 pKa = 3.95 VALLEE182 pKa = 4.9 KK183 pKa = 10.69 EE184 pKa = 4.99 DD185 pKa = 3.77 GSQGKK190 pKa = 7.81 KK191 pKa = 6.51 TKK193 pKa = 10.14 KK194 pKa = 8.65 RR195 pKa = 11.84 TRR197 pKa = 11.84 ASPQTSGKK205 pKa = 9.48 RR206 pKa = 11.84 RR207 pKa = 11.84 YY208 pKa = 9.61 RR209 pKa = 11.84 GPRR212 pKa = 11.84 GQDD215 pKa = 2.64 RR216 pKa = 11.84 AA217 pKa = 3.8
Molecular weight: 24.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.779
IPC2_protein 8.946
IPC_protein 9.019
Toseland 9.253
ProMoST 9.194
Dawson 9.604
Bjellqvist 9.37
Wikipedia 9.867
Rodwell 9.706
Grimsley 9.736
Solomon 9.677
Lehninger 9.619
Nozaki 9.209
DTASelect 9.37
Thurlkill 9.399
EMBOSS 9.692
Sillero 9.516
Patrickios 4.584
IPC_peptide 9.663
IPC2_peptide 8.009
IPC2.peptide.svr19 7.847
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1861
160
636
310.2
34.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.598 ± 1.713
2.149 ± 0.588
4.782 ± 0.658
7.792 ± 0.653
3.385 ± 0.472
7.899 ± 1.18
1.934 ± 0.363
3.815 ± 0.771
4.89 ± 0.808
8.866 ± 0.994
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.203 ± 0.387
3.332 ± 0.632
7.577 ± 1.4
4.03 ± 0.57
5.75 ± 0.541
6.663 ± 0.92
5.75 ± 0.581
5.75 ± 0.933
1.343 ± 0.514
3.493 ± 0.381
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here