Simian torque teno virus 30
Average proteome isoelectric point is 8.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5IMW1|A0A0C5IMW1_9VIRU ORF2 OS=Simian torque teno virus 30 OX=1619218 PE=4 SV=1
MM1 pKa = 7.02 YY2 pKa = 10.15 FRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.47 AKK11 pKa = 9.86 LGSVGDD17 pKa = 4.08 YY18 pKa = 11.3 LEE20 pKa = 5.19 LSHH23 pKa = 7.37 PGLPTGTPRR32 pKa = 11.84 AMAKK36 pKa = 9.96 RR37 pKa = 11.84 PRR39 pKa = 11.84 EE40 pKa = 4.13 TYY42 pKa = 7.36 EE43 pKa = 4.07 TTSKK47 pKa = 10.64 EE48 pKa = 3.86 AATWLHH54 pKa = 6.13 LVGEE58 pKa = 4.68 THH60 pKa = 7.5 DD61 pKa = 3.84 LWCPCGKK68 pKa = 9.54 PLPHH72 pKa = 7.13 LSALLNTVGAATTTSTCRR90 pKa = 11.84 TGGEE94 pKa = 4.0 DD95 pKa = 3.1 AAGGGRR101 pKa = 11.84 AAGAGPATEE110 pKa = 6.31 GGDD113 pKa = 3.19 GWDD116 pKa = 5.5 DD117 pKa = 3.54 INLDD121 pKa = 3.71 EE122 pKa = 5.6 LLADD126 pKa = 4.24 VAAEE130 pKa = 3.9 DD131 pKa = 4.34 AEE133 pKa = 4.34 RR134 pKa = 5.2
Molecular weight: 14.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.606
IPC2_protein 5.588
IPC_protein 5.563
Toseland 5.868
ProMoST 5.868
Dawson 5.753
Bjellqvist 5.779
Wikipedia 5.741
Rodwell 5.728
Grimsley 5.982
Solomon 5.753
Lehninger 5.741
Nozaki 5.995
DTASelect 6.173
Thurlkill 6.135
EMBOSS 6.097
Sillero 6.084
Patrickios 4.291
IPC_peptide 5.766
IPC2_peptide 6.097
IPC2.peptide.svr19 6.098
Protein with the highest isoelectric point:
>tr|A0A0C5IMW1|A0A0C5IMW1_9VIRU ORF2 OS=Simian torque teno virus 30 OX=1619218 PE=4 SV=1
MM1 pKa = 7.6 PYY3 pKa = 7.68 WWRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 WPRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 WTRR20 pKa = 11.84 YY21 pKa = 7.92 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 WMGRR29 pKa = 11.84 YY30 pKa = 8.84 KK31 pKa = 10.3 PRR33 pKa = 11.84 RR34 pKa = 11.84 ATRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 TVRR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 FSGYY54 pKa = 6.63 TRR56 pKa = 11.84 RR57 pKa = 11.84 ATRR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 GRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 RR67 pKa = 11.84 RR68 pKa = 11.84 LTVTLKK74 pKa = 9.2 QWKK77 pKa = 9.32 PEE79 pKa = 3.96 TLRR82 pKa = 11.84 SLRR85 pKa = 11.84 VTGLFPLIVCGSGASRR101 pKa = 11.84 NNWIQRR107 pKa = 11.84 SFDD110 pKa = 3.43 VPGPDD115 pKa = 3.43 TPIGGNLSVSVWTMRR130 pKa = 11.84 VLYY133 pKa = 9.83 EE134 pKa = 3.58 EE135 pKa = 4.5 WQLHH139 pKa = 5.24 RR140 pKa = 11.84 NNWSRR145 pKa = 11.84 TNSDD149 pKa = 3.65 LDD151 pKa = 3.42 LVMYY155 pKa = 7.81 YY156 pKa = 11.04 GCTLKK161 pKa = 10.52 FWRR164 pKa = 11.84 HH165 pKa = 5.74 PYY167 pKa = 9.55 VDD169 pKa = 4.46 YY170 pKa = 10.37 IVKK173 pKa = 10.44 YY174 pKa = 10.42 IRR176 pKa = 11.84 NTPFKK181 pKa = 10.48 TEE183 pKa = 4.07 EE184 pKa = 3.87 LTHH187 pKa = 6.43 AAGHH191 pKa = 6.21 PMVLMLSKK199 pKa = 10.29 HH200 pKa = 5.42 HH201 pKa = 6.31 VVVPSLLTKK210 pKa = 8.84 PHH212 pKa = 4.8 GRR214 pKa = 11.84 RR215 pKa = 11.84 WIKK218 pKa = 9.24 VTVRR222 pKa = 11.84 PPRR225 pKa = 11.84 MLTSKK230 pKa = 10.56 FYY232 pKa = 10.56 FQSDD236 pKa = 3.69 FCNVNLLGIVASACKK251 pKa = 10.34 LNSPWLEE258 pKa = 3.73 PDD260 pKa = 4.28 KK261 pKa = 10.48 ITPCITFYY269 pKa = 11.44 ALKK272 pKa = 9.24 NTVFKK277 pKa = 10.78 NQNITEE283 pKa = 4.13 AQNAYY288 pKa = 7.72 TALTAIIVQNKK299 pKa = 7.48 EE300 pKa = 3.39 MAYY303 pKa = 8.44 NTWLEE308 pKa = 4.15 HH309 pKa = 5.51 HH310 pKa = 6.85 WGSFGNGKK318 pKa = 9.87 AFNITNVKK326 pKa = 10.44 DD327 pKa = 3.79 NSTLTQNQINTLRR340 pKa = 11.84 QTAEE344 pKa = 3.64 NTFKK348 pKa = 10.91 SRR350 pKa = 11.84 YY351 pKa = 7.57 NFIYY355 pKa = 9.86 EE356 pKa = 4.2 AQPPTTFTYY365 pKa = 11.11 YY366 pKa = 10.88 NLHH369 pKa = 5.31 GWGLYY374 pKa = 10.02 SPLWLDD380 pKa = 3.42 PDD382 pKa = 4.21 RR383 pKa = 11.84 IEE385 pKa = 4.92 PEE387 pKa = 3.79 SEE389 pKa = 3.72 AAFLAVRR396 pKa = 11.84 YY397 pKa = 9.9 NPLNDD402 pKa = 3.56 KK403 pKa = 11.64 GEE405 pKa = 4.47 GNWVALQPLTKK416 pKa = 9.87 EE417 pKa = 4.5 LPILDD422 pKa = 4.53 DD423 pKa = 3.51 TCKK426 pKa = 10.0 MVLKK430 pKa = 10.28 DD431 pKa = 3.7 YY432 pKa = 10.21 PLWLILYY439 pKa = 7.08 GYY441 pKa = 9.01 PDD443 pKa = 4.42 AATKK447 pKa = 10.12 ILSGSSPLNNTRR459 pKa = 11.84 LIIRR463 pKa = 11.84 CQYY466 pKa = 9.6 TDD468 pKa = 3.88 PPLAFPQATDD478 pKa = 3.25 YY479 pKa = 11.22 KK480 pKa = 10.01 RR481 pKa = 11.84 GYY483 pKa = 8.43 TIYY486 pKa = 10.83 SRR488 pKa = 11.84 DD489 pKa = 3.43 FATGRR494 pKa = 11.84 MPGGNTFIPITTARR508 pKa = 11.84 LWYY511 pKa = 9.44 PRR513 pKa = 11.84 LQNQLDD519 pKa = 3.85 VVEE522 pKa = 5.8 AIVNCGPLMPRR533 pKa = 11.84 DD534 pKa = 4.4 DD535 pKa = 4.7 LHH537 pKa = 7.64 QGWAVTMGYY546 pKa = 10.29 KK547 pKa = 10.22 YY548 pKa = 10.47 RR549 pKa = 11.84 FQLGGNLPPKK559 pKa = 9.87 QDD561 pKa = 3.49 PVDD564 pKa = 3.64 PCKK567 pKa = 10.74 VPKK570 pKa = 10.29 RR571 pKa = 11.84 EE572 pKa = 4.22 LPDD575 pKa = 3.8 PSAEE579 pKa = 3.95 LTAVQIVDD587 pKa = 4.16 PATMDD592 pKa = 4.0 PLSTLHH598 pKa = 6.26 PWDD601 pKa = 3.77 YY602 pKa = 10.46 RR603 pKa = 11.84 RR604 pKa = 11.84 SMLTSSGIKK613 pKa = 10.02 RR614 pKa = 11.84 MSADD618 pKa = 3.81 RR619 pKa = 11.84 EE620 pKa = 4.43 TDD622 pKa = 2.98 SSLYY626 pKa = 10.26 PGATGVAKK634 pKa = 10.38 RR635 pKa = 11.84 PKK637 pKa = 9.92 TDD639 pKa = 2.72 VPTQKK644 pKa = 10.73 GEE646 pKa = 4.03 EE647 pKa = 4.16 PGPGEE652 pKa = 4.05 RR653 pKa = 11.84 LRR655 pKa = 11.84 EE656 pKa = 3.99 VLQSLLEE663 pKa = 4.07 EE664 pKa = 4.28 QQQEE668 pKa = 4.6 TEE670 pKa = 4.97 DD671 pKa = 4.32 PPSPPPGEE679 pKa = 4.49 RR680 pKa = 11.84 VQQPEE685 pKa = 4.52 LFQQLALRR693 pKa = 11.84 YY694 pKa = 8.85 QLQQQRR700 pKa = 11.84 EE701 pKa = 4.28 RR702 pKa = 11.84 QGKK705 pKa = 8.09 LAQGIKK711 pKa = 11.01 YY712 pKa = 7.92 MFQQLIKK719 pKa = 8.17 TQQGVQVDD727 pKa = 3.9 PRR729 pKa = 11.84 LLL731 pKa = 4.26
Molecular weight: 85.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.633
IPC_protein 10.218
Toseland 10.204
ProMoST 9.984
Dawson 10.423
Bjellqvist 10.145
Wikipedia 10.643
Rodwell 10.657
Grimsley 10.511
Solomon 10.467
Lehninger 10.423
Nozaki 10.218
DTASelect 10.145
Thurlkill 10.277
EMBOSS 10.613
Sillero 10.335
Patrickios 10.072
IPC_peptide 10.452
IPC2_peptide 9.092
IPC2.peptide.svr19 8.634
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
990
125
731
330.0
37.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.061 ± 1.993
1.717 ± 0.833
4.444 ± 0.717
4.646 ± 0.75
2.424 ± 0.865
6.465 ± 1.645
1.919 ± 0.403
3.232 ± 1.068
5.152 ± 1.101
8.99 ± 1.525
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.919 ± 0.438
3.939 ± 1.034
7.677 ± 0.579
5.455 ± 1.89
10.202 ± 0.881
6.364 ± 3.995
8.081 ± 0.62
4.646 ± 1.044
2.828 ± 0.264
3.838 ± 1.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here