Malassezia sympodialis (strain ATCC 42132) (Atopic eczema-associated yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Malasseziomycetes; Malasseziales; Malasseziaceae; Malassezia; Malassezia sympodialis

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M8A3B6|A0A1M8A3B6_MALS4 Uncharacterized protein OS=Malassezia sympodialis (strain ATCC 42132) OX=1230383 GN=MSYG_1299 PE=4 SV=1
MM1 pKa = 7.3AVSYY5 pKa = 10.71YY6 pKa = 10.93DD7 pKa = 4.0EE8 pKa = 6.04IEE10 pKa = 4.46LEE12 pKa = 4.21DD13 pKa = 3.59MEE15 pKa = 5.19FDD17 pKa = 3.31EE18 pKa = 5.77GKK20 pKa = 9.97QAFTYY25 pKa = 8.74PCPCGDD31 pKa = 3.36KK32 pKa = 11.11FEE34 pKa = 5.17ISLEE38 pKa = 3.95QLKK41 pKa = 10.75NEE43 pKa = 4.14EE44 pKa = 4.81DD45 pKa = 3.37IARR48 pKa = 11.84CPSCTLLIRR57 pKa = 11.84VIYY60 pKa = 10.43DD61 pKa = 3.48PLDD64 pKa = 3.6FDD66 pKa = 4.39

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M8A1W2|A0A1M8A1W2_MALS4 Similar to S.cerevisiae protein RCF1 (Cytochrome c oxidase subunit) OS=Malassezia sympodialis (strain ATCC 42132) OX=1230383 GN=MSYG_0519 PE=4 SV=1
MM1 pKa = 6.93NTPGLVAVAQAFVRR15 pKa = 11.84PGVLVPHH22 pKa = 6.46LQVASLRR29 pKa = 11.84LIDD32 pKa = 3.63WRR34 pKa = 11.84QVRR37 pKa = 11.84ASGVRR42 pKa = 11.84YY43 pKa = 9.43IVYY46 pKa = 10.61DD47 pKa = 3.61KK48 pKa = 11.04DD49 pKa = 3.46NCLTRR54 pKa = 11.84PHH56 pKa = 6.97HH57 pKa = 7.08DD58 pKa = 3.28ALAPALEE65 pKa = 4.39ATWDD69 pKa = 3.29EE70 pKa = 4.52CKK72 pKa = 10.68AVFGEE77 pKa = 4.27EE78 pKa = 3.63NLLIVSNSAGSSQDD92 pKa = 3.27PTGLAAEE99 pKa = 4.29QVSSKK104 pKa = 10.84LGVPILCHH112 pKa = 5.86AVKK115 pKa = 10.62KK116 pKa = 10.2PGRR119 pKa = 11.84ACAQQVVDD127 pKa = 4.56YY128 pKa = 10.71VASQHH133 pKa = 6.28LRR135 pKa = 11.84SPDD138 pKa = 3.23AAHH141 pKa = 7.26PPRR144 pKa = 11.84LLVVGDD150 pKa = 5.1RR151 pKa = 11.84ITTDD155 pKa = 2.75MAFSYY160 pKa = 10.49RR161 pKa = 11.84IASLLGRR168 pKa = 11.84AYY170 pKa = 9.51PHH172 pKa = 6.72EE173 pKa = 5.58RR174 pKa = 11.84DD175 pKa = 3.28LCIGVLTHH183 pKa = 6.24EE184 pKa = 4.11LWGRR188 pKa = 11.84EE189 pKa = 3.93QLGTRR194 pKa = 11.84VMRR197 pKa = 11.84MLEE200 pKa = 4.09NTVLRR205 pKa = 11.84QLVRR209 pKa = 11.84VGIPPGGTWTARR221 pKa = 11.84GAPSPVCSAWVRR233 pKa = 11.84AVAPADD239 pKa = 3.87AAPLPAPRR247 pKa = 11.84KK248 pKa = 9.82ARR250 pKa = 11.84THH252 pKa = 5.25MRR254 pKa = 11.84WLAPLVDD261 pKa = 3.74AWRR264 pKa = 11.84TIVRR268 pKa = 11.84EE269 pKa = 3.96TFGSVRR275 pKa = 11.84RR276 pKa = 11.84VRR278 pKa = 11.84EE279 pKa = 4.24LTWSVCTNAPTRR291 pKa = 11.84SWRR294 pKa = 11.84STWRR298 pKa = 11.84KK299 pKa = 7.09PQRR302 pKa = 11.84PPPAPWSRR310 pKa = 11.84SMSTSARR317 pKa = 11.84ARR319 pKa = 11.84GALHH323 pKa = 6.81EE324 pKa = 4.56KK325 pKa = 9.54PVPRR329 pKa = 11.84RR330 pKa = 11.84VPTADD335 pKa = 3.16APRR338 pKa = 11.84APRR341 pKa = 11.84AVPTWLGIPRR351 pKa = 11.84IRR353 pKa = 11.84WLMALATLVLLPLGFMGGMKK373 pKa = 10.37LSEE376 pKa = 4.11LVEE379 pKa = 3.93RR380 pKa = 11.84WRR382 pKa = 11.84IGDD385 pKa = 3.8LSHH388 pKa = 6.86EE389 pKa = 4.43GEE391 pKa = 4.58ARR393 pKa = 11.84VDD395 pKa = 3.74APPPAPAPEE404 pKa = 4.67PDD406 pKa = 3.44VEE408 pKa = 4.33EE409 pKa = 4.3TQVQLRR415 pKa = 11.84KK416 pKa = 10.03KK417 pKa = 9.97ISSLEE422 pKa = 3.94LEE424 pKa = 4.54HH425 pKa = 6.42FHH427 pKa = 6.8LRR429 pKa = 11.84RR430 pKa = 11.84EE431 pKa = 4.01RR432 pKa = 11.84SVLDD436 pKa = 3.83DD437 pKa = 3.5KK438 pKa = 11.2LARR441 pKa = 11.84LAARR445 pKa = 11.84PAA447 pKa = 3.4

Molecular weight: 49.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4490

11

4501

2239772

34

4822

497.6

54.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.956 ± 0.08

1.226 ± 0.013

5.667 ± 0.026

6.03 ± 0.033

3.15 ± 0.021

6.107 ± 0.032

2.936 ± 0.02

3.62 ± 0.028

3.536 ± 0.037

9.986 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.015

2.585 ± 0.027

6.601 ± 0.042

4.214 ± 0.021

7.114 ± 0.034

7.48 ± 0.043

5.523 ± 0.02

6.766 ± 0.025

1.459 ± 0.013

2.538 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski