Impatiens necrotic spot virus (INSV)
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q01268|MVP_INSV Movement protein OS=Impatiens necrotic spot virus OX=11612 PE=3 SV=1
MM1 pKa = 7.5 ALKK4 pKa = 9.09 EE5 pKa = 4.13 TDD7 pKa = 3.12 AKK9 pKa = 10.65 IHH11 pKa = 5.38 VEE13 pKa = 4.04 RR14 pKa = 11.84 GDD16 pKa = 3.39 HH17 pKa = 6.65 PEE19 pKa = 4.62 IYY21 pKa = 10.51 DD22 pKa = 3.32 EE23 pKa = 4.74 AYY25 pKa = 9.53 YY26 pKa = 10.81 DD27 pKa = 4.18 RR28 pKa = 11.84 SVDD31 pKa = 3.56 HH32 pKa = 6.96 KK33 pKa = 11.71 NEE35 pKa = 3.91 ILDD38 pKa = 3.82 TLAEE42 pKa = 4.09 MLQNATGKK50 pKa = 7.06 TLRR53 pKa = 11.84 PTRR56 pKa = 11.84 DD57 pKa = 3.33 TQTVLANNEE66 pKa = 4.16 VPQSSSGLSSTPTTISIMDD85 pKa = 4.59 LPNPCLNASSLTCSIKK101 pKa = 10.62 GVSTFNVYY109 pKa = 10.22 YY110 pKa = 9.94 QVEE113 pKa = 4.75 SNGVIYY119 pKa = 10.66 SCISDD124 pKa = 4.49 TITKK128 pKa = 10.21 LGNCEE133 pKa = 4.02 GSSEE137 pKa = 4.11 LPRR140 pKa = 11.84 SFEE143 pKa = 4.21 TVPVVPITKK152 pKa = 9.22 IDD154 pKa = 3.73 NKK156 pKa = 10.33 RR157 pKa = 11.84 KK158 pKa = 10.08 LSIGTKK164 pKa = 9.78 FYY166 pKa = 10.47 IIEE169 pKa = 4.07 SLEE172 pKa = 4.07 NYY174 pKa = 9.35 NYY176 pKa = 10.33 PIMYY180 pKa = 10.06 NSRR183 pKa = 11.84 PTNGTVSLQSVKK195 pKa = 10.37 FSGDD199 pKa = 3.39 CKK201 pKa = 10.19 ISKK204 pKa = 9.05 TNIVNSYY211 pKa = 8.38 TVSLTTPEE219 pKa = 4.65 KK220 pKa = 10.36 IMGYY224 pKa = 6.84 VVKK227 pKa = 10.74 RR228 pKa = 11.84 EE229 pKa = 3.98 GSDD232 pKa = 3.15 MSHH235 pKa = 7.26 SIISFSGSVSLTFTEE250 pKa = 4.27 EE251 pKa = 4.06 NMDD254 pKa = 3.58 GKK256 pKa = 10.31 HH257 pKa = 6.47 NLLCGDD263 pKa = 4.15 KK264 pKa = 10.33 SSKK267 pKa = 10.44 VPLVDD272 pKa = 4.11 KK273 pKa = 10.66 RR274 pKa = 11.84 VRR276 pKa = 11.84 DD277 pKa = 4.21 CIIKK281 pKa = 9.92 YY282 pKa = 10.26 SKK284 pKa = 10.79 NIYY287 pKa = 9.4 KK288 pKa = 8.29 QTACINFSWFRR299 pKa = 11.84 LIMIALIVYY308 pKa = 7.54 FPIRR312 pKa = 11.84 YY313 pKa = 8.5 LVNKK317 pKa = 8.41 TSKK320 pKa = 9.24 TLFYY324 pKa = 10.53 GYY326 pKa = 10.61 DD327 pKa = 3.51 LLGLITYY334 pKa = 7.77 PILLLINYY342 pKa = 7.32 LWSYY346 pKa = 10.77 FPLKK350 pKa = 10.76 CKK352 pKa = 10.73 VCGNLCLVTHH362 pKa = 6.93 EE363 pKa = 4.52 CSKK366 pKa = 11.31 LCICNKK372 pKa = 9.76 NKK374 pKa = 10.29 ASEE377 pKa = 4.16 EE378 pKa = 3.99 HH379 pKa = 7.07 SEE381 pKa = 4.05 EE382 pKa = 4.33 CPIITRR388 pKa = 11.84 TAEE391 pKa = 3.92 KK392 pKa = 10.15 NKK394 pKa = 9.75 KK395 pKa = 8.79 YY396 pKa = 10.53 NWASIEE402 pKa = 4.06 WFHH405 pKa = 7.61 LIVNTKK411 pKa = 10.01 IGLSFLKK418 pKa = 10.62 AVTEE422 pKa = 4.06 TLIGFLILSQMPMSMAQTAQCLDD445 pKa = 2.97 SCYY448 pKa = 10.46 YY449 pKa = 10.91 VPGCDD454 pKa = 2.32 RR455 pKa = 11.84 FVTNRR460 pKa = 11.84 YY461 pKa = 8.59 DD462 pKa = 3.21 KK463 pKa = 11.24 CPEE466 pKa = 3.91 KK467 pKa = 10.75 DD468 pKa = 3.21 QCFCAIKK475 pKa = 10.07 EE476 pKa = 4.05 NSIVEE481 pKa = 4.63 SNFLTNVVTEE491 pKa = 4.61 GPMDD495 pKa = 5.18 CIPYY499 pKa = 9.07 QEE501 pKa = 4.41 CKK503 pKa = 10.82 GRR505 pKa = 11.84 ITEE508 pKa = 4.09 NALVTFVKK516 pKa = 10.4 CRR518 pKa = 11.84 FGCEE522 pKa = 3.71 YY523 pKa = 11.3 ASIFQSKK530 pKa = 9.64 PLDD533 pKa = 3.75 NGFLEE538 pKa = 4.42 YY539 pKa = 10.95 SGDD542 pKa = 3.86 TLGLNAVNLHH552 pKa = 4.84 FMKK555 pKa = 10.75 RR556 pKa = 11.84 LRR558 pKa = 11.84 NGIIDD563 pKa = 4.45 FYY565 pKa = 11.59 NKK567 pKa = 8.26 TEE569 pKa = 3.88 KK570 pKa = 10.57 YY571 pKa = 10.71 GYY573 pKa = 10.07 ISGDD577 pKa = 3.16 ALKK580 pKa = 10.85 SNEE583 pKa = 3.86 SDD585 pKa = 2.97 IPEE588 pKa = 4.59 SIFPRR593 pKa = 11.84 KK594 pKa = 9.73 SLIFDD599 pKa = 3.5 SVIDD603 pKa = 3.76 GKK605 pKa = 10.34 YY606 pKa = 9.67 RR607 pKa = 11.84 YY608 pKa = 9.11 MIEE611 pKa = 4.0 EE612 pKa = 4.28 SLLSGGGTVFSLNDD626 pKa = 3.62 KK627 pKa = 10.76 SSSTAQKK634 pKa = 9.78 FVVYY638 pKa = 9.74 IKK640 pKa = 10.38 KK641 pKa = 9.93 VRR643 pKa = 11.84 IQYY646 pKa = 9.9 DD647 pKa = 3.22 VSEE650 pKa = 4.73 QYY652 pKa = 7.67 TTAPIQSTHH661 pKa = 5.61 TDD663 pKa = 3.8 FFSTCTGKK671 pKa = 10.88 CSDD674 pKa = 4.38 CRR676 pKa = 11.84 KK677 pKa = 8.46 EE678 pKa = 4.01 QPITGYY684 pKa = 10.63 QDD686 pKa = 3.33 FCITPTSYY694 pKa = 9.62 WGCEE698 pKa = 4.11 EE699 pKa = 4.3 VWCLAINEE707 pKa = 4.4 GATCGFCRR715 pKa = 11.84 NVYY718 pKa = 11.13 DD719 pKa = 3.45 MDD721 pKa = 3.6 QSFRR725 pKa = 11.84 IYY727 pKa = 10.86 SVIKK731 pKa = 9.22 STIKK735 pKa = 10.72 SEE737 pKa = 4.06 VCISGFVGAKK747 pKa = 9.44 CFTVSEE753 pKa = 4.44 EE754 pKa = 4.22 VPSEE758 pKa = 4.0 SGYY761 pKa = 10.39 FQADD765 pKa = 3.04 ILADD769 pKa = 3.62 FHH771 pKa = 8.0 NDD773 pKa = 2.74 GLTIGQLIAHH783 pKa = 7.06 GPDD786 pKa = 2.82 SHH788 pKa = 8.61 VYY790 pKa = 9.58 AGNIARR796 pKa = 11.84 LNNPSKK802 pKa = 10.61 MFGHH806 pKa = 7.02 PQLSHH811 pKa = 6.7 QGDD814 pKa = 4.2 PIFSKK819 pKa = 9.6 KK820 pKa = 8.86 TLDD823 pKa = 3.75 TNDD826 pKa = 4.41 LSWDD830 pKa = 3.46 CSAIGKK836 pKa = 8.21 KK837 pKa = 9.01 TITIKK842 pKa = 10.49 SCGYY846 pKa = 8.39 DD847 pKa = 2.98 TYY849 pKa = 11.34 RR850 pKa = 11.84 FKK852 pKa = 10.79 TGLNQISDD860 pKa = 3.76 IPVQFTDD867 pKa = 3.21 QNSFYY872 pKa = 9.67 MEE874 pKa = 4.96 KK875 pKa = 10.06 IFSLGKK881 pKa = 10.4 LKK883 pKa = 10.36 IVLDD887 pKa = 4.15 LPSEE891 pKa = 4.15 LFKK894 pKa = 10.56 TVPKK898 pKa = 10.58 KK899 pKa = 10.55 PILSSVSLSCKK910 pKa = 10.43 GCFLCSQGLRR920 pKa = 11.84 CAASFISDD928 pKa = 3.16 ITFSARR934 pKa = 11.84 LTMKK938 pKa = 9.95 QCSLSTYY945 pKa = 10.14 QIAVKK950 pKa = 10.49 KK951 pKa = 10.2 GANKK955 pKa = 10.31 YY956 pKa = 9.72 NLTMFCTSNPEE967 pKa = 3.89 KK968 pKa = 10.32 QKK970 pKa = 10.68 MIIEE974 pKa = 4.3 PEE976 pKa = 3.94 GDD978 pKa = 2.84 KK979 pKa = 10.99 SYY981 pKa = 11.38 SVEE984 pKa = 3.92 ALVDD988 pKa = 3.94 SVAVLEE994 pKa = 4.54 PEE996 pKa = 4.99 NIIDD1000 pKa = 4.54 QNDD1003 pKa = 3.33 QHH1005 pKa = 7.97 AHH1007 pKa = 6.35 EE1008 pKa = 4.39 EE1009 pKa = 3.97 QQYY1012 pKa = 11.41 NSDD1015 pKa = 3.46 TSVWSFWDD1023 pKa = 3.81 YY1024 pKa = 11.48 VKK1026 pKa = 11.19 SPFNFIASHH1035 pKa = 6.48 FGSFFDD1041 pKa = 3.63 TVRR1044 pKa = 11.84 VVLLILFVFALAYY1057 pKa = 10.1 LCSIVATMCRR1067 pKa = 11.84 GYY1069 pKa = 10.84 VRR1071 pKa = 11.84 NKK1073 pKa = 10.21 SYY1075 pKa = 8.32 KK1076 pKa = 8.2 TKK1078 pKa = 10.78 YY1079 pKa = 10.15 IEE1081 pKa = 4.29 DD1082 pKa = 3.9 TNDD1085 pKa = 2.96 YY1086 pKa = 10.38 SLVSTSSGKK1095 pKa = 8.74 DD1096 pKa = 2.89 TITRR1100 pKa = 11.84 RR1101 pKa = 11.84 RR1102 pKa = 11.84 PPLDD1106 pKa = 3.3 FSGII1110 pKa = 3.76
Molecular weight: 124.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.031
IPC2_protein 6.287
IPC_protein 6.275
Toseland 6.249
ProMoST 6.751
Dawson 6.722
Bjellqvist 6.795
Wikipedia 6.693
Rodwell 6.722
Grimsley 6.287
Solomon 6.722
Lehninger 6.722
Nozaki 7.088
DTASelect 7.088
Thurlkill 7.117
EMBOSS 7.088
Sillero 7.161
Patrickios 3.427
IPC_peptide 6.737
IPC2_peptide 6.81
IPC2.peptide.svr19 6.751
Protein with the highest isoelectric point:
>sp|Q01268|MVP_INSV Movement protein OS=Impatiens necrotic spot virus OX=11612 PE=3 SV=1
MM1 pKa = 7.48 NSFFKK6 pKa = 10.63 SLRR9 pKa = 11.84 SSSSRR14 pKa = 11.84 EE15 pKa = 3.47 LDD17 pKa = 3.28 HH18 pKa = 7.32 PRR20 pKa = 11.84 VTTTLSKK27 pKa = 10.5 QGADD31 pKa = 2.45 IVVHH35 pKa = 6.1 NPSANHH41 pKa = 5.76 NNKK44 pKa = 9.51 EE45 pKa = 4.1 VLQRR49 pKa = 11.84 AMDD52 pKa = 3.77 SSKK55 pKa = 11.32 GKK57 pKa = 10.18 ILMNNTGTSSLGTYY71 pKa = 10.02 EE72 pKa = 4.11 SDD74 pKa = 3.4 QISEE78 pKa = 4.31 SEE80 pKa = 4.44 SYY82 pKa = 10.89 DD83 pKa = 3.18 LSARR87 pKa = 11.84 MIVDD91 pKa = 3.93 TNHH94 pKa = 7.28 HH95 pKa = 5.74 ISSWKK100 pKa = 9.82 NDD102 pKa = 3.42 LFVGNGDD109 pKa = 3.39 KK110 pKa = 10.99 AATKK114 pKa = 9.89 IIKK117 pKa = 9.32 IHH119 pKa = 5.57 PTWDD123 pKa = 3.06 SRR125 pKa = 11.84 KK126 pKa = 8.54 QYY128 pKa = 10.8 MMISRR133 pKa = 11.84 IVIWICPTIADD144 pKa = 4.47 PDD146 pKa = 3.85 GKK148 pKa = 10.69 LAVALIDD155 pKa = 3.91 PNKK158 pKa = 10.21 SVNARR163 pKa = 11.84 TVLKK167 pKa = 10.4 GQGSIKK173 pKa = 10.74 DD174 pKa = 3.77 PICFVFYY181 pKa = 10.61 LNWSIPKK188 pKa = 9.92 VNNTSEE194 pKa = 4.29 NCVQLHH200 pKa = 6.15 LLCDD204 pKa = 3.46 QVYY207 pKa = 10.33 KK208 pKa = 10.68 KK209 pKa = 10.4 DD210 pKa = 3.56 VSFASVMYY218 pKa = 9.84 SWTKK222 pKa = 9.77 EE223 pKa = 3.95 FCDD226 pKa = 4.72 SPRR229 pKa = 11.84 ADD231 pKa = 4.0 LDD233 pKa = 3.82 KK234 pKa = 11.18 SCMIIPINRR243 pKa = 11.84 AIRR246 pKa = 11.84 AKK248 pKa = 9.89 SQAFIEE254 pKa = 4.45 ACKK257 pKa = 10.62 LIIPKK262 pKa = 10.35 GNSEE266 pKa = 3.96 KK267 pKa = 10.61 QIRR270 pKa = 11.84 RR271 pKa = 11.84 QLAEE275 pKa = 3.85 LSANLEE281 pKa = 3.98 KK282 pKa = 10.85 SVEE285 pKa = 4.1 EE286 pKa = 4.31 EE287 pKa = 4.85 EE288 pKa = 4.85 NVTDD292 pKa = 3.94 NKK294 pKa = 10.67 IEE296 pKa = 3.8 ISFDD300 pKa = 3.43 NEE302 pKa = 3.48 II303 pKa = 4.09
Molecular weight: 34.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.661
IPC2_protein 6.825
IPC_protein 6.898
Toseland 6.751
ProMoST 7.38
Dawson 7.527
Bjellqvist 7.6
Wikipedia 7.454
Rodwell 7.527
Grimsley 6.825
Solomon 7.585
Lehninger 7.614
Nozaki 7.951
DTASelect 7.702
Thurlkill 7.746
EMBOSS 7.79
Sillero 7.995
Patrickios 4.533
IPC_peptide 7.585
IPC2_peptide 7.117
IPC2.peptide.svr19 7.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1413
303
1110
706.5
79.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.317 ± 0.808
3.68 ± 0.682
5.662 ± 0.468
5.308 ± 0.151
4.6 ± 0.812
4.954 ± 0.823
1.769 ± 0.269
8.209 ± 0.514
7.502 ± 0.373
7.431 ± 0.413
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.052 ± 0.293
5.662 ± 0.796
3.822 ± 0.095
3.114 ± 0.093
3.397 ± 0.445
10.616 ± 0.466
6.582 ± 0.977
6.016 ± 0.037
0.991 ± 0.328
4.317 ± 1.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here