Red clover powdery mildew-associated totivirus 2
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3Q2X1|A0A0S3Q2X1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 2 OX=1714363 GN=RdRp PE=3 SV=1
MM1 pKa = 8.05 DD2 pKa = 4.69 SMVFDD7 pKa = 3.69 TCVDD11 pKa = 3.43 NVTSLLYY18 pKa = 10.58 NLLRR22 pKa = 11.84 MYY24 pKa = 10.74 YY25 pKa = 9.71 ILEE28 pKa = 4.26 NKK30 pKa = 9.17 NLSVKK35 pKa = 9.49 KK36 pKa = 9.0 TDD38 pKa = 2.91 SYY40 pKa = 11.77 YY41 pKa = 10.92 DD42 pKa = 4.14 DD43 pKa = 3.82 GHH45 pKa = 8.39 LSVDD49 pKa = 3.17 MGDD52 pKa = 3.22 VFGVGEE58 pKa = 4.03 RR59 pKa = 11.84 SKK61 pKa = 10.97 RR62 pKa = 11.84 ITDD65 pKa = 3.28 VRR67 pKa = 11.84 FNPCRR72 pKa = 11.84 AHH74 pKa = 7.34 RR75 pKa = 11.84 YY76 pKa = 8.76 DD77 pKa = 3.84 AEE79 pKa = 4.64 TYY81 pKa = 9.53 FLNFSGEE88 pKa = 4.43 PVLHH92 pKa = 6.15 MANANALSSGILATALGEE110 pKa = 4.28 WSSEE114 pKa = 3.75 TPYY117 pKa = 10.75 AISHH121 pKa = 7.01 PSPALAQNIIINTRR135 pKa = 11.84 GGVSQDD141 pKa = 3.46 EE142 pKa = 4.3 IEE144 pKa = 4.58 SFNQITTNDD153 pKa = 3.44 VLKK156 pKa = 10.54 VIKK159 pKa = 10.44 DD160 pKa = 3.31 IVEE163 pKa = 4.19 SNRR166 pKa = 11.84 YY167 pKa = 8.02 HH168 pKa = 6.47 AQFEE172 pKa = 4.34 LAYY175 pKa = 11.22 LMLAQLLFTLKK186 pKa = 10.12 PRR188 pKa = 11.84 SAEE191 pKa = 3.74 ALIWMHH197 pKa = 6.01 GTPLMQLPKK206 pKa = 10.73 FMTFRR211 pKa = 11.84 GLVNEE216 pKa = 4.49 TVSLAPYY223 pKa = 9.95 VPFPEE228 pKa = 5.26 RR229 pKa = 11.84 LDD231 pKa = 3.82 TYY233 pKa = 8.94 GTWLANKK240 pKa = 9.0 EE241 pKa = 3.99 AIYY244 pKa = 10.4 YY245 pKa = 10.22 HH246 pKa = 6.49 SLLINEE252 pKa = 4.52 VVMTYY257 pKa = 10.3 AYY259 pKa = 9.97 EE260 pKa = 5.13 AITTNDD266 pKa = 2.65 ITRR269 pKa = 11.84 LLLRR273 pKa = 11.84 QIGGIFSAEE282 pKa = 4.07 PGLMADD288 pKa = 4.29 MSCFAQVLQKK298 pKa = 10.75 EE299 pKa = 4.44 VLLPFEE305 pKa = 4.69 TNGGLDD311 pKa = 3.0 RR312 pKa = 11.84 FTWINCLDD320 pKa = 4.05 GEE322 pKa = 4.35 IGMQVKK328 pKa = 10.41 VNDD331 pKa = 4.23 PNAQHH336 pKa = 7.22 HH337 pKa = 6.09 YY338 pKa = 10.68 RR339 pKa = 11.84 LDD341 pKa = 3.65 TLEE344 pKa = 5.56 LDD346 pKa = 4.32 DD347 pKa = 4.65 GVIRR351 pKa = 11.84 HH352 pKa = 5.79 TLRR355 pKa = 11.84 LDD357 pKa = 3.29 SFVPYY362 pKa = 10.09 VYY364 pKa = 10.15 PVLLYY369 pKa = 10.48 GYY371 pKa = 10.07 APSTYY376 pKa = 9.32 YY377 pKa = 10.96 QNDD380 pKa = 3.69 CVRR383 pKa = 11.84 EE384 pKa = 3.96 VEE386 pKa = 4.29 MKK388 pKa = 10.05 IDD390 pKa = 3.7 SKK392 pKa = 10.48 KK393 pKa = 10.55 EE394 pKa = 3.7 RR395 pKa = 11.84 MITEE399 pKa = 4.21 SVDD402 pKa = 3.86 DD403 pKa = 4.2 FSKK406 pKa = 11.27 VMNILRR412 pKa = 11.84 MAGYY416 pKa = 9.64 DD417 pKa = 3.41 ATGGSCYY424 pKa = 10.57 EE425 pKa = 4.02 GDD427 pKa = 4.58 KK428 pKa = 10.51 VKK430 pKa = 10.83 NWADD434 pKa = 3.15 NSSGHH439 pKa = 5.97 FLYY442 pKa = 10.33 TRR444 pKa = 11.84 SAEE447 pKa = 4.22 APDD450 pKa = 5.03 DD451 pKa = 4.76 DD452 pKa = 4.97 IFYY455 pKa = 10.99 VDD457 pKa = 4.68 LGLEE461 pKa = 4.22 EE462 pKa = 5.04 RR463 pKa = 11.84 SKK465 pKa = 11.08 SWVDD469 pKa = 3.47 EE470 pKa = 4.22 PRR472 pKa = 11.84 WSEE475 pKa = 4.1 KK476 pKa = 10.34 ACLRR480 pKa = 11.84 SEE482 pKa = 4.35 VKK484 pKa = 9.04 ITAFTMFKK492 pKa = 10.62 DD493 pKa = 3.26 GVMYY497 pKa = 10.9 KK498 pKa = 10.63 NITGVLKK505 pKa = 10.43 KK506 pKa = 10.83 YY507 pKa = 9.97 SFKK510 pKa = 10.88 QPSLEE515 pKa = 3.82 ITSKK519 pKa = 8.75 HH520 pKa = 3.92 TRR522 pKa = 11.84 EE523 pKa = 4.19 VKK525 pKa = 9.11 VTLRR529 pKa = 11.84 PRR531 pKa = 11.84 PHH533 pKa = 6.43 IKK535 pKa = 10.29 AGFRR539 pKa = 11.84 MARR542 pKa = 11.84 IDD544 pKa = 4.51 DD545 pKa = 3.85 RR546 pKa = 11.84 TILHH550 pKa = 6.83 PVTNTLFGDD559 pKa = 4.45 LAQSDD564 pKa = 4.66 EE565 pKa = 4.03 QLDD568 pKa = 3.96 WYY570 pKa = 10.65 DD571 pKa = 3.4 QDD573 pKa = 4.83 QEE575 pKa = 4.78 DD576 pKa = 4.24 GTGAA580 pKa = 3.47
Molecular weight: 66.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.951
IPC2_protein 5.016
IPC_protein 4.991
Toseland 4.94
ProMoST 5.143
Dawson 5.003
Bjellqvist 5.13
Wikipedia 4.889
Rodwell 4.902
Grimsley 4.863
Solomon 5.003
Lehninger 4.952
Nozaki 5.118
DTASelect 5.308
Thurlkill 4.94
EMBOSS 4.94
Sillero 5.194
Patrickios 3.77
IPC_peptide 5.003
IPC2_peptide 5.181
IPC2.peptide.svr19 5.132
Protein with the highest isoelectric point:
>tr|A0A0S3Q2X1|A0A0S3Q2X1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 2 OX=1714363 GN=RdRp PE=3 SV=1
MM1 pKa = 7.82 LITVYY6 pKa = 10.77 ILMGLRR12 pKa = 11.84 TRR14 pKa = 11.84 GAVDD18 pKa = 3.26 EE19 pKa = 4.79 ALLNVKK25 pKa = 9.57 ALHH28 pKa = 6.45 KK29 pKa = 10.8 LHH31 pKa = 6.07 GQYY34 pKa = 11.01 VLAIPVLFGNVIAYY48 pKa = 9.61 YY49 pKa = 10.23 VDD51 pKa = 3.15 KK52 pKa = 10.99 HH53 pKa = 6.43 DD54 pKa = 3.84 VTMADD59 pKa = 3.36 KK60 pKa = 10.74 KK61 pKa = 9.99 VAKK64 pKa = 9.89 QAGLVIANNVFVDD77 pKa = 4.74 FCPDD81 pKa = 2.84 ARR83 pKa = 11.84 VDD85 pKa = 3.84 YY86 pKa = 10.22 IAVMGNMAKK95 pKa = 10.36 GLHH98 pKa = 6.04 KK99 pKa = 9.66 KK100 pKa = 7.74 TRR102 pKa = 11.84 INDD105 pKa = 3.53 RR106 pKa = 11.84 EE107 pKa = 4.37 LIRR110 pKa = 11.84 SRR112 pKa = 11.84 ISQRR116 pKa = 11.84 HH117 pKa = 4.8 HH118 pKa = 4.37 MHH120 pKa = 6.46 IKK122 pKa = 10.42 VGMLDD127 pKa = 3.57 SEE129 pKa = 4.33 GDD131 pKa = 3.59 EE132 pKa = 4.96 NEE134 pKa = 4.16 IEE136 pKa = 4.01 LDD138 pKa = 3.75 GVKK141 pKa = 10.43 GYY143 pKa = 10.65 IMHH146 pKa = 7.71 RR147 pKa = 11.84 LMKK150 pKa = 8.33 MTTVRR155 pKa = 11.84 QTVTTFLLYY164 pKa = 10.77 VEE166 pKa = 5.6 LAPSWAADD174 pKa = 3.41 VLAVIVSRR182 pKa = 11.84 HH183 pKa = 5.1 SEE185 pKa = 3.67 WAQLMDD191 pKa = 4.26 DD192 pKa = 4.49 LKK194 pKa = 11.09 LFSMYY199 pKa = 10.01 VKK201 pKa = 10.35 QYY203 pKa = 8.45 QHH205 pKa = 6.85 VIRR208 pKa = 11.84 SDD210 pKa = 3.53 LAVLFEE216 pKa = 5.2 LNVLANRR223 pKa = 11.84 SDD225 pKa = 4.94 SEE227 pKa = 4.22 MDD229 pKa = 2.96 WAADD233 pKa = 3.0 IAKK236 pKa = 8.28 RR237 pKa = 11.84 TKK239 pKa = 10.68 SVVATPDD246 pKa = 3.07 QEE248 pKa = 4.24 RR249 pKa = 11.84 LYY251 pKa = 10.8 RR252 pKa = 11.84 DD253 pKa = 3.66 GVSMFTSARR262 pKa = 11.84 AEE264 pKa = 4.22 GKK266 pKa = 10.13 QPVTMKK272 pKa = 9.93 WEE274 pKa = 4.57 EE275 pKa = 4.0 YY276 pKa = 10.98 RR277 pKa = 11.84 NMTWGLTPPGAVHH290 pKa = 6.61 SPEE293 pKa = 3.71 GDD295 pKa = 3.14 FRR297 pKa = 11.84 RR298 pKa = 11.84 NISTIPSKK306 pKa = 10.83 LRR308 pKa = 11.84 NKK310 pKa = 8.61 KK311 pKa = 8.27 TYY313 pKa = 10.79 NSVFRR318 pKa = 11.84 PKK320 pKa = 10.26 EE321 pKa = 3.79 LKK323 pKa = 10.2 HH324 pKa = 7.45 YY325 pKa = 7.33 MAQRR329 pKa = 11.84 PKK331 pKa = 10.3 IIAKK335 pKa = 10.06 PSVKK339 pKa = 10.66 YY340 pKa = 9.97 EE341 pKa = 3.7 WGKK344 pKa = 9.93 NRR346 pKa = 11.84 ALYY349 pKa = 10.66 GCDD352 pKa = 3.4 LVSHH356 pKa = 6.54 MMTDD360 pKa = 3.87 FGMMQAEE367 pKa = 4.39 STLPSWMPTGRR378 pKa = 11.84 MADD381 pKa = 3.48 EE382 pKa = 4.27 NRR384 pKa = 11.84 VKK386 pKa = 10.68 RR387 pKa = 11.84 QIATMGNGIPFCYY400 pKa = 10.51 DD401 pKa = 2.8 FDD403 pKa = 4.94 DD404 pKa = 4.89 FNSQHH409 pKa = 5.81 SVKK412 pKa = 10.75 SMQTVITAWYY422 pKa = 9.13 SVYY425 pKa = 11.08 GDD427 pKa = 5.26 LISDD431 pKa = 3.84 EE432 pKa = 4.44 QKK434 pKa = 11.15 LCVDD438 pKa = 4.04 WVVQSLEE445 pKa = 3.92 TQLIINSVGLGSSKK459 pKa = 10.72 YY460 pKa = 9.92 IEE462 pKa = 3.96 VDD464 pKa = 3.04 GTLFSGWRR472 pKa = 11.84 LTSFMNTCLNYY483 pKa = 10.7 LYY485 pKa = 10.73 LEE487 pKa = 4.19 QANVTKK493 pKa = 9.94 RR494 pKa = 11.84 TVGNIHH500 pKa = 6.59 NGDD503 pKa = 3.51 DD504 pKa = 3.49 VYY506 pKa = 11.99 ANIRR510 pKa = 11.84 TIGDD514 pKa = 3.59 ALDD517 pKa = 3.18 IMRR520 pKa = 11.84 DD521 pKa = 3.35 AKK523 pKa = 11.03 SIGVRR528 pKa = 11.84 AQMTKK533 pKa = 10.09 TNIGTIGEE541 pKa = 4.27 FLRR544 pKa = 11.84 IDD546 pKa = 3.84 NKK548 pKa = 11.13 AVDD551 pKa = 3.8 ATGAQYY557 pKa = 9.18 LTRR560 pKa = 11.84 SCATAVHH567 pKa = 6.17 GRR569 pKa = 11.84 IEE571 pKa = 4.56 SDD573 pKa = 3.46 TPNSTRR579 pKa = 11.84 SVVEE583 pKa = 3.57 ANMIRR588 pKa = 11.84 LEE590 pKa = 3.96 ALKK593 pKa = 10.92 ARR595 pKa = 11.84 GGDD598 pKa = 3.56 SNKK601 pKa = 10.16 IKK603 pKa = 10.54 VIKK606 pKa = 10.44 DD607 pKa = 3.26 NMISRR612 pKa = 11.84 IARR615 pKa = 11.84 KK616 pKa = 9.32 HH617 pKa = 5.31 DD618 pKa = 3.35 TSEE621 pKa = 3.78 EE622 pKa = 4.39 TICDD626 pKa = 4.3 LIEE629 pKa = 4.14 TPKK632 pKa = 11.18 ALGGLKK638 pKa = 10.1 DD639 pKa = 4.23 DD640 pKa = 4.78 NKK642 pKa = 9.95 PVPWRR647 pKa = 11.84 LIIKK651 pKa = 10.5 LEE653 pKa = 4.22 GEE655 pKa = 4.51 LDD657 pKa = 3.51 SMAYY661 pKa = 9.87 EE662 pKa = 4.93 LANKK666 pKa = 7.91 MKK668 pKa = 10.53 KK669 pKa = 9.93 AVNNYY674 pKa = 8.66 HH675 pKa = 7.2 KK676 pKa = 10.31 AVCDD680 pKa = 3.59 MLEE683 pKa = 4.02 VEE685 pKa = 4.97 MPTGYY690 pKa = 11.41 DD691 pKa = 3.05 MLNGINEE698 pKa = 4.19 GMLQTKK704 pKa = 9.7 KK705 pKa = 9.91 MRR707 pKa = 11.84 IEE709 pKa = 4.37 KK710 pKa = 10.23 IKK712 pKa = 10.52 IDD714 pKa = 3.3 IKK716 pKa = 10.64 LAARR720 pKa = 11.84 ICAMHH725 pKa = 6.6 KK726 pKa = 10.19 AWGGSGDD733 pKa = 3.65 GGMAATMRR741 pKa = 11.84 IFTDD745 pKa = 2.66 IKK747 pKa = 8.12 MHH749 pKa = 6.16 RR750 pKa = 11.84 KK751 pKa = 8.74 HH752 pKa = 6.67 RR753 pKa = 11.84 RR754 pKa = 11.84 LRR756 pKa = 11.84 DD757 pKa = 3.26 QVRR760 pKa = 11.84 RR761 pKa = 11.84 LVKK764 pKa = 10.76 SGDD767 pKa = 3.2 FNLWFNTVYY776 pKa = 11.07
Molecular weight: 87.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.553
IPC2_protein 8.463
IPC_protein 8.331
Toseland 9.004
ProMoST 8.916
Dawson 9.341
Bjellqvist 9.165
Wikipedia 9.502
Rodwell 9.56
Grimsley 9.385
Solomon 9.37
Lehninger 9.341
Nozaki 9.268
DTASelect 9.077
Thurlkill 9.209
EMBOSS 9.487
Sillero 9.355
Patrickios 4.774
IPC_peptide 9.37
IPC2_peptide 7.761
IPC2.peptide.svr19 7.689
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1356
580
776
678.0
77.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.932 ± 0.477
1.18 ± 0.018
7.153 ± 0.285
5.383 ± 0.542
3.171 ± 0.636
6.047 ± 0.235
2.581 ± 0.11
6.121 ± 0.511
6.268 ± 0.948
8.776 ± 0.692
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.499 ± 0.691
4.794 ± 0.091
3.171 ± 0.523
2.876 ± 0.036
5.9 ± 0.479
5.973 ± 0.381
6.195 ± 0.235
7.227 ± 0.331
1.549 ± 0.111
4.204 ± 0.638
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here