Jatropha yellow mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6DXE5|B6DXE5_9GEMI Capsid protein OS=Jatropha yellow mosaic virus OX=1485012 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.38FPEE11 pKa = 4.68TVHH14 pKa = 7.0GFRR17 pKa = 11.84CMLAVKK23 pKa = 9.87YY24 pKa = 10.06LQLVEE29 pKa = 4.35KK30 pKa = 9.32TYY32 pKa = 11.38SPDD35 pKa = 3.08TLGYY39 pKa = 10.84DD40 pKa = 4.83LIRR43 pKa = 11.84DD44 pKa = 4.71LISVIRR50 pKa = 11.84ARR52 pKa = 11.84NYY54 pKa = 9.49VEE56 pKa = 3.52ATSRR60 pKa = 11.84YY61 pKa = 6.98NHH63 pKa = 5.62FHH65 pKa = 6.55ARR67 pKa = 11.84LEE69 pKa = 4.22GTPPSQLRR77 pKa = 11.84QLICEE82 pKa = 4.49PCCCPHH88 pKa = 7.46CPRR91 pKa = 11.84QQSKK95 pKa = 11.24SMGEE99 pKa = 3.79QADD102 pKa = 3.84EE103 pKa = 4.18QKK105 pKa = 11.12AQDD108 pKa = 3.57VQAVQKK114 pKa = 10.44SGCSS118 pKa = 3.21

Molecular weight: 13.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6DXE7|B6DXE7_9GEMI Transcriptional activator protein OS=Jatropha yellow mosaic virus OX=1485012 PE=3 SV=2
MM1 pKa = 8.22DD2 pKa = 4.53SRR4 pKa = 11.84TGEE7 pKa = 4.61PITAARR13 pKa = 11.84FNNGVFIWTVPNPLYY28 pKa = 10.74FRR30 pKa = 11.84VPVHH34 pKa = 5.82MSRR37 pKa = 11.84PFHH40 pKa = 6.65LEE42 pKa = 2.93QDD44 pKa = 3.75IIHH47 pKa = 6.26IQIQFNHH54 pKa = 6.39NLRR57 pKa = 11.84QALQIHH63 pKa = 6.24KK64 pKa = 9.72CFLSFKK70 pKa = 8.64VWTRR74 pKa = 11.84SRR76 pKa = 11.84IPTGSFLRR84 pKa = 11.84VFKK87 pKa = 9.78TQVIRR92 pKa = 11.84YY93 pKa = 8.39LDD95 pKa = 3.66RR96 pKa = 11.84LGVISINLVIRR107 pKa = 11.84AVDD110 pKa = 3.34HH111 pKa = 5.62VLYY114 pKa = 10.9NVLHH118 pKa = 6.82HH119 pKa = 7.1DD120 pKa = 4.08ANVEE124 pKa = 3.85QSNEE128 pKa = 3.91IIFRR132 pKa = 11.84LYY134 pKa = 10.85

Molecular weight: 15.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

97

361

183.3

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.545 ± 0.611

2.091 ± 0.641

4.727 ± 0.321

4.909 ± 0.743

4.545 ± 0.627

4.636 ± 0.369

3.818 ± 0.595

5.0 ± 0.774

5.545 ± 0.877

7.364 ± 0.793

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.515

5.455 ± 0.676

6.0 ± 0.497

5.455 ± 0.812

6.636 ± 0.894

8.909 ± 1.087

5.455 ± 0.796

6.364 ± 1.288

1.455 ± 0.127

3.818 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski