Amino acid dipepetide frequency for bacterium HR33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.072AlaAla: 17.072 ± 0.202	0.92AlaCys: 0.92 ± 0.042	5.09AlaAsp: 5.09 ± 0.075	7.998AlaGlu: 7.998 ± 0.113	3.733AlaPhe: 3.733 ± 0.067	11.021AlaGly: 11.021 ± 0.129	1.798AlaHis: 1.798 ± 0.051	5.313AlaIle: 5.313 ± 0.095	2.756AlaLys: 2.756 ± 0.06	13.247AlaLeu: 13.247 ± 0.15	2.206AlaMet: 2.206 ± 0.054	2.0AlaAsn: 2.0 ± 0.053	5.182AlaPro: 5.182 ± 0.085	3.616AlaGln: 3.616 ± 0.07	10.269AlaArg: 10.269 ± 0.142	5.772AlaSer: 5.772 ± 0.076	4.876AlaThr: 4.876 ± 0.073	10.623AlaVal: 10.623 ± 0.144	1.604AlaTrp: 1.604 ± 0.046	2.527AlaTyr: 2.527 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.835CysAla: 0.835 ± 0.034	0.088CysCys: 0.088 ± 0.01	0.399CysAsp: 0.399 ± 0.022	0.445CysGlu: 0.445 ± 0.021	0.256CysPhe: 0.256 ± 0.017	0.953CysGly: 0.953 ± 0.042	0.28CysHis: 0.28 ± 0.033	0.28CysIle: 0.28 ± 0.019	0.161CysLys: 0.161 ± 0.015	0.608CysLeu: 0.608 ± 0.025	0.097CysMet: 0.097 ± 0.009	0.188CysAsn: 0.188 ± 0.014	0.496CysPro: 0.496 ± 0.027	0.196CysGln: 0.196 ± 0.013	0.662CysArg: 0.662 ± 0.029	0.47CysSer: 0.47 ± 0.023	0.354CysThr: 0.354 ± 0.024	0.517CysVal: 0.517 ± 0.025	0.134CysTrp: 0.134 ± 0.02	0.219CysTyr: 0.219 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.851AspAla: 4.851 ± 0.074	0.358AspCys: 0.358 ± 0.02	1.998AspAsp: 1.998 ± 0.047	2.75AspGlu: 2.75 ± 0.059	1.855AspPhe: 1.855 ± 0.044	4.348AspGly: 4.348 ± 0.079	0.841AspHis: 0.841 ± 0.031	2.034AspIle: 2.034 ± 0.052	0.774AspLys: 0.774 ± 0.032	6.071AspLeu: 6.071 ± 0.088	0.618AspMet: 0.618 ± 0.029	0.864AspAsn: 0.864 ± 0.037	4.155AspPro: 4.155 ± 0.076	1.417AspGln: 1.417 ± 0.04	4.946AspArg: 4.946 ± 0.086	2.814AspSer: 2.814 ± 0.062	2.334AspThr: 2.334 ± 0.06	3.549AspVal: 3.549 ± 0.068	0.795AspTrp: 0.795 ± 0.034	1.269AspTyr: 1.269 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	8.0GluAla: 8.0 ± 0.111	0.383GluCys: 0.383 ± 0.023	2.78GluAsp: 2.78 ± 0.06	5.183GluGlu: 5.183 ± 0.1	2.113GluPhe: 2.113 ± 0.05	5.043GluGly: 5.043 ± 0.085	1.318GluHis: 1.318 ± 0.033	3.756GluIle: 3.756 ± 0.065	1.773GluLys: 1.773 ± 0.054	8.299GluLeu: 8.299 ± 0.101	1.197GluMet: 1.197 ± 0.033	1.345GluAsn: 1.345 ± 0.042	3.403GluPro: 3.403 ± 0.071	2.364GluGln: 2.364 ± 0.058	7.479GluArg: 7.479 ± 0.099	2.789GluSer: 2.789 ± 0.053	2.819GluThr: 2.819 ± 0.056	5.449GluVal: 5.449 ± 0.092	0.957GluTrp: 0.957 ± 0.032	1.47GluTyr: 1.47 ± 0.041	0.0GluXaa: 0.0 ± 0.0
Phe	3.685PheAla: 3.685 ± 0.075	0.303PheCys: 0.303 ± 0.019	2.1PheAsp: 2.1 ± 0.053	2.303PheGlu: 2.303 ± 0.056	1.408PhePhe: 1.408 ± 0.045	3.409PheGly: 3.409 ± 0.064	0.649PheHis: 0.649 ± 0.023	1.251PheIle: 1.251 ± 0.037	0.879PheLys: 0.879 ± 0.028	3.471PheLeu: 3.471 ± 0.07	0.546PheMet: 0.546 ± 0.024	0.816PheAsn: 0.816 ± 0.032	1.784PhePro: 1.784 ± 0.045	1.004PheGln: 1.004 ± 0.034	2.856PheArg: 2.856 ± 0.061	2.119PheSer: 2.119 ± 0.055	1.824PheThr: 1.824 ± 0.049	2.732PheVal: 2.732 ± 0.053	0.589PheTrp: 0.589 ± 0.028	1.054PheTyr: 1.054 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	9.313GlyAla: 9.313 ± 0.122	0.834GlyCys: 0.834 ± 0.035	4.232GlyAsp: 4.232 ± 0.081	5.833GlyGlu: 5.833 ± 0.087	3.354GlyPhe: 3.354 ± 0.061	8.044GlyGly: 8.044 ± 0.122	1.466GlyHis: 1.466 ± 0.043	4.451GlyIle: 4.451 ± 0.073	2.731GlyLys: 2.731 ± 0.065	8.565GlyLeu: 8.565 ± 0.115	1.951GlyMet: 1.951 ± 0.051	1.997GlyAsn: 1.997 ± 0.05	4.189GlyPro: 4.189 ± 0.069	2.426GlyGln: 2.426 ± 0.061	7.657GlyArg: 7.657 ± 0.1	5.367GlySer: 5.367 ± 0.086	4.923GlyThr: 4.923 ± 0.09	7.04GlyVal: 7.04 ± 0.092	1.543GlyTrp: 1.543 ± 0.048	2.488GlyTyr: 2.488 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.71HisAla: 1.71 ± 0.039	0.196HisCys: 0.196 ± 0.014	0.831HisAsp: 0.831 ± 0.028	0.956HisGlu: 0.956 ± 0.033	0.679HisPhe: 0.679 ± 0.028	1.545HisGly: 1.545 ± 0.042	0.474HisHis: 0.474 ± 0.02	0.709HisIle: 0.709 ± 0.028	0.342HisLys: 0.342 ± 0.02	2.04HisLeu: 2.04 ± 0.049	0.255HisMet: 0.255 ± 0.018	0.408HisAsn: 0.408 ± 0.021	1.512HisPro: 1.512 ± 0.044	0.526HisGln: 0.526 ± 0.026	1.754HisArg: 1.754 ± 0.048	0.91HisSer: 0.91 ± 0.029	0.745HisThr: 0.745 ± 0.027	1.268HisVal: 1.268 ± 0.032	0.301HisTrp: 0.301 ± 0.018	0.598HisTyr: 0.598 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.768IleAla: 5.768 ± 0.095	0.373IleCys: 0.373 ± 0.019	2.517IleAsp: 2.517 ± 0.048	3.238IleGlu: 3.238 ± 0.068	1.445IlePhe: 1.445 ± 0.039	3.914IleGly: 3.914 ± 0.072	0.827IleHis: 0.827 ± 0.03	1.426IleIle: 1.426 ± 0.042	1.134IleLys: 1.134 ± 0.038	4.218IleLeu: 4.218 ± 0.071	0.648IleMet: 0.648 ± 0.031	0.997IleAsn: 0.997 ± 0.038	2.607IlePro: 2.607 ± 0.053	1.225IleGln: 1.225 ± 0.041	3.668IleArg: 3.668 ± 0.065	2.355IleSer: 2.355 ± 0.053	2.325IleThr: 2.325 ± 0.055	3.773IleVal: 3.773 ± 0.067	0.62IleTrp: 0.62 ± 0.029	1.213IleTyr: 1.213 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	2.839LysAla: 2.839 ± 0.065	0.158LysCys: 0.158 ± 0.015	1.124LysAsp: 1.124 ± 0.04	1.799LysGlu: 1.799 ± 0.048	0.646LysPhe: 0.646 ± 0.03	1.98LysGly: 1.98 ± 0.055	0.417LysHis: 0.417 ± 0.02	1.413LysIle: 1.413 ± 0.041	0.9LysLys: 0.9 ± 0.038	2.892LysLeu: 2.892 ± 0.059	0.452LysMet: 0.452 ± 0.018	0.548LysAsn: 0.548 ± 0.027	1.473LysPro: 1.473 ± 0.048	0.734LysGln: 0.734 ± 0.028	2.347LysArg: 2.347 ± 0.052	1.175LysSer: 1.175 ± 0.038	1.298LysThr: 1.298 ± 0.04	2.159LysVal: 2.159 ± 0.058	0.308LysTrp: 0.308 ± 0.019	0.563LysTyr: 0.563 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	14.409LeuAla: 14.409 ± 0.151	0.754LeuCys: 0.754 ± 0.03	5.829LeuAsp: 5.829 ± 0.081	8.407LeuGlu: 8.407 ± 0.121	3.411LeuPhe: 3.411 ± 0.073	9.712LeuGly: 9.712 ± 0.128	1.783LeuHis: 1.783 ± 0.05	4.06LeuIle: 4.06 ± 0.072	3.38LeuLys: 3.38 ± 0.061	11.218LeuLeu: 11.218 ± 0.154	1.627LeuMet: 1.627 ± 0.044	2.264LeuAsn: 2.264 ± 0.051	5.88LeuPro: 5.88 ± 0.08	2.65LeuGln: 2.65 ± 0.048	9.016LeuArg: 9.016 ± 0.115	6.023LeuSer: 6.023 ± 0.084	4.84LeuThr: 4.84 ± 0.081	8.997LeuVal: 8.997 ± 0.121	1.544LeuTrp: 1.544 ± 0.044	2.426LeuTyr: 2.426 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.196MetAla: 2.196 ± 0.057	0.107MetCys: 0.107 ± 0.011	0.772MetAsp: 0.772 ± 0.028	1.01MetGlu: 1.01 ± 0.034	0.443MetPhe: 0.443 ± 0.023	1.435MetGly: 1.435 ± 0.045	0.306MetHis: 0.306 ± 0.022	0.859MetIle: 0.859 ± 0.03	0.655MetLys: 0.655 ± 0.022	1.818MetLeu: 1.818 ± 0.049	0.323MetMet: 0.323 ± 0.019	0.526MetAsn: 0.526 ± 0.023	1.035MetPro: 1.035 ± 0.036	0.504MetGln: 0.504 ± 0.024	1.591MetArg: 1.591 ± 0.043	1.083MetSer: 1.083 ± 0.04	0.974MetThr: 0.974 ± 0.033	1.459MetVal: 1.459 ± 0.046	0.231MetTrp: 0.231 ± 0.016	0.321MetTyr: 0.321 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.184AsnAla: 2.184 ± 0.05	0.19AsnCys: 0.19 ± 0.013	0.932AsnAsp: 0.932 ± 0.038	0.932AsnGlu: 0.932 ± 0.032	0.8AsnPhe: 0.8 ± 0.034	1.698AsnGly: 1.698 ± 0.045	0.409AsnHis: 0.409 ± 0.022	0.891AsnIle: 0.891 ± 0.039	0.349AsnLys: 0.349 ± 0.02	2.575AsnLeu: 2.575 ± 0.06	0.307AsnMet: 0.307 ± 0.017	0.541AsnAsn: 0.541 ± 0.031	1.878AsnPro: 1.878 ± 0.043	0.691AsnGln: 0.691 ± 0.027	2.069AsnArg: 2.069 ± 0.048	0.985AsnSer: 0.985 ± 0.04	1.011AsnThr: 1.011 ± 0.036	1.817AsnVal: 1.817 ± 0.051	0.382AsnTrp: 0.382 ± 0.021	0.611AsnTyr: 0.611 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	6.467ProAla: 6.467 ± 0.094	0.335ProCys: 0.335 ± 0.02	3.579ProAsp: 3.579 ± 0.065	4.958ProGlu: 4.958 ± 0.077	1.972ProPhe: 1.972 ± 0.05	5.687ProGly: 5.687 ± 0.094	1.018ProHis: 1.018 ± 0.032	2.16ProIle: 2.16 ± 0.05	1.375ProLys: 1.375 ± 0.044	5.27ProLeu: 5.27 ± 0.086	0.961ProMet: 0.961 ± 0.033	1.257ProAsn: 1.257 ± 0.036	3.525ProPro: 3.525 ± 0.082	1.786ProGln: 1.786 ± 0.047	4.07ProArg: 4.07 ± 0.073	2.815ProSer: 2.815 ± 0.054	2.278ProThr: 2.278 ± 0.053	4.714ProVal: 4.714 ± 0.076	0.889ProTrp: 0.889 ± 0.034	1.387ProTyr: 1.387 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.496GlnAla: 3.496 ± 0.072	0.173GlnCys: 0.173 ± 0.014	1.171GlnAsp: 1.171 ± 0.038	1.875GlnGlu: 1.875 ± 0.054	0.994GlnPhe: 0.994 ± 0.03	2.311GlnGly: 2.311 ± 0.055	0.538GlnHis: 0.538 ± 0.024	1.397GlnIle: 1.397 ± 0.042	0.707GlnLys: 0.707 ± 0.027	3.38GlnLeu: 3.38 ± 0.077	0.59GlnMet: 0.59 ± 0.025	0.649GlnAsn: 0.649 ± 0.028	1.721GlnPro: 1.721 ± 0.045	1.151GlnGln: 1.151 ± 0.041	3.001GlnArg: 3.001 ± 0.067	1.393GlnSer: 1.393 ± 0.035	1.295GlnThr: 1.295 ± 0.041	2.407GlnVal: 2.407 ± 0.058	0.42GlnTrp: 0.42 ± 0.021	0.688GlnTyr: 0.688 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	9.723ArgAla: 9.723 ± 0.129	0.692ArgCys: 0.692 ± 0.03	4.286ArgAsp: 4.286 ± 0.077	6.574ArgGlu: 6.574 ± 0.098	3.416ArgPhe: 3.416 ± 0.069	6.746ArgGly: 6.746 ± 0.095	1.62ArgHis: 1.62 ± 0.037	4.425ArgIle: 4.425 ± 0.074	2.237ArgLys: 2.237 ± 0.047	9.869ArgLeu: 9.869 ± 0.124	1.997ArgMet: 1.997 ± 0.052	1.869ArgAsn: 1.869 ± 0.047	4.621ArgPro: 4.621 ± 0.084	2.808ArgGln: 2.808 ± 0.058	9.035ArgArg: 9.035 ± 0.133	4.912ArgSer: 4.912 ± 0.079	3.876ArgThr: 3.876 ± 0.066	7.084ArgVal: 7.084 ± 0.093	1.676ArgTrp: 1.676 ± 0.05	2.743ArgTyr: 2.743 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	5.806SerAla: 5.806 ± 0.078	0.42SerCys: 0.42 ± 0.023	2.609SerAsp: 2.609 ± 0.053	2.963SerGlu: 2.963 ± 0.063	1.988SerPhe: 1.988 ± 0.052	5.93SerGly: 5.93 ± 0.086	0.883SerHis: 0.883 ± 0.03	2.081SerIle: 2.081 ± 0.045	1.177SerLys: 1.177 ± 0.036	5.766SerLeu: 5.766 ± 0.09	0.943SerMet: 0.943 ± 0.033	1.065SerAsn: 1.065 ± 0.04	3.328SerPro: 3.328 ± 0.074	1.535SerGln: 1.535 ± 0.037	4.594SerArg: 4.594 ± 0.071	2.873SerSer: 2.873 ± 0.057	2.404SerThr: 2.404 ± 0.056	4.228SerVal: 4.228 ± 0.078	0.911SerTrp: 0.911 ± 0.035	1.404SerTyr: 1.404 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	5.588ThrAla: 5.588 ± 0.092	0.337ThrCys: 0.337 ± 0.023	2.281ThrAsp: 2.281 ± 0.049	2.422ThrGlu: 2.422 ± 0.057	1.694ThrPhe: 1.694 ± 0.046	4.77ThrGly: 4.77 ± 0.088	0.837ThrHis: 0.837 ± 0.03	2.167ThrIle: 2.167 ± 0.055	0.997ThrLys: 0.997 ± 0.039	5.261ThrLeu: 5.261 ± 0.074	0.839ThrMet: 0.839 ± 0.032	0.995ThrAsn: 0.995 ± 0.037	3.006ThrPro: 3.006 ± 0.06	1.131ThrGln: 1.131 ± 0.037	3.402ThrArg: 3.402 ± 0.06	2.242ThrSer: 2.242 ± 0.059	2.364ThrThr: 2.364 ± 0.058	4.661ThrVal: 4.661 ± 0.074	0.783ThrTrp: 0.783 ± 0.04	1.208ThrTyr: 1.208 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.772ValAla: 9.772 ± 0.127	0.612ValCys: 0.612 ± 0.03	4.034ValAsp: 4.034 ± 0.067	5.917ValGlu: 5.917 ± 0.1	2.898ValPhe: 2.898 ± 0.064	6.444ValGly: 6.444 ± 0.095	1.413ValHis: 1.413 ± 0.035	3.886ValIle: 3.886 ± 0.062	2.046ValLys: 2.046 ± 0.05	8.939ValLeu: 8.939 ± 0.096	1.425ValMet: 1.425 ± 0.044	1.919ValAsn: 1.919 ± 0.053	4.587ValPro: 4.587 ± 0.075	2.231ValGln: 2.231 ± 0.055	7.309ValArg: 7.309 ± 0.09	4.556ValSer: 4.556 ± 0.072	4.454ValThr: 4.454 ± 0.074	7.744ValVal: 7.744 ± 0.112	1.174ValTrp: 1.174 ± 0.045	2.075ValTyr: 2.075 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	1.361TrpAla: 1.361 ± 0.038	0.134TrpCys: 0.134 ± 0.012	0.706TrpAsp: 0.706 ± 0.027	0.935TrpGlu: 0.935 ± 0.029	0.674TrpPhe: 0.674 ± 0.028	1.095TrpGly: 1.095 ± 0.047	0.326TrpHis: 0.326 ± 0.018	0.867TrpIle: 0.867 ± 0.036	0.424TrpLys: 0.424 ± 0.022	1.787TrpLeu: 1.787 ± 0.048	0.298TrpMet: 0.298 ± 0.019	0.433TrpAsn: 0.433 ± 0.022	0.731TrpPro: 0.731 ± 0.03	0.492TrpGln: 0.492 ± 0.022	1.532TrpArg: 1.532 ± 0.044	0.993TrpSer: 0.993 ± 0.042	0.791TrpThr: 0.791 ± 0.034	1.281TrpVal: 1.281 ± 0.044	0.311TrpTrp: 0.311 ± 0.02	0.456TrpTyr: 0.456 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.55TyrAla: 2.55 ± 0.051	0.273TyrCys: 0.273 ± 0.023	1.369TyrAsp: 1.369 ± 0.038	1.423TyrGlu: 1.423 ± 0.044	1.005TyrPhe: 1.005 ± 0.033	2.317TyrGly: 2.317 ± 0.057	0.556TyrHis: 0.556 ± 0.024	0.864TyrIle: 0.864 ± 0.031	0.429TyrLys: 0.429 ± 0.023	2.933TyrLeu: 2.933 ± 0.061	0.369TyrMet: 0.369 ± 0.021	0.57TyrAsn: 0.57 ± 0.026	1.391TyrPro: 1.391 ± 0.047	0.867TyrGln: 0.867 ± 0.031	2.951TyrArg: 2.951 ± 0.058	1.26TyrSer: 1.26 ± 0.043	1.201TyrThr: 1.201 ± 0.034	1.935TyrVal: 1.935 ± 0.045	0.456TyrTrp: 0.456 ± 0.025	0.838TyrTyr: 0.838 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2661 proteins (916511 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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