Streptococcus satellite phage Javan462

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZPJ8|A0A4D5ZPJ8_9VIRU DUF4065 domain-containing protein OS=Streptococcus satellite phage Javan462 OX=2558713 GN=JavanS462_0022 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.68TPKK9 pKa = 10.71QFMFTYY15 pKa = 9.75QCKK18 pKa = 10.39DD19 pKa = 2.96YY20 pKa = 9.71DD21 pKa = 4.03TARR24 pKa = 11.84VTSTAILGYY33 pKa = 8.7ITGTYY38 pKa = 7.79EE39 pKa = 3.86QNLAEE44 pKa = 4.27ATLNGDD50 pKa = 3.7GYY52 pKa = 11.66LEE54 pKa = 3.88VTYY57 pKa = 10.94FEE59 pKa = 5.3DD60 pKa = 4.58KK61 pKa = 10.97SINFNLKK68 pKa = 10.17RR69 pKa = 11.84ICDD72 pKa = 3.8SFKK75 pKa = 10.88DD76 pKa = 3.81YY77 pKa = 11.31CNQPEE82 pKa = 4.1DD83 pKa = 3.82MEE85 pKa = 4.59GEE87 pKa = 4.27KK88 pKa = 10.75

Molecular weight: 10.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZPI8|A0A4D5ZPI8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan462 OX=2558713 GN=JavanS462_0012 PE=4 SV=1
MM1 pKa = 7.56LGGAFMIFSILKK13 pKa = 9.03EE14 pKa = 3.87YY15 pKa = 10.98KK16 pKa = 9.67SFKK19 pKa = 10.83NEE21 pKa = 3.78VAEE24 pKa = 5.21HH25 pKa = 6.39RR26 pKa = 11.84VEE28 pKa = 4.08QCKK31 pKa = 8.72KK32 pKa = 8.34TEE34 pKa = 3.83KK35 pKa = 9.9LHH37 pKa = 7.48RR38 pKa = 11.84NFLKK42 pKa = 10.27VKK44 pKa = 9.96RR45 pKa = 11.84RR46 pKa = 11.84NEE48 pKa = 3.86EE49 pKa = 3.49FRR51 pKa = 11.84KK52 pKa = 10.5EE53 pKa = 3.21FDD55 pKa = 3.36EE56 pKa = 4.29RR57 pKa = 11.84FKK59 pKa = 10.91RR60 pKa = 11.84YY61 pKa = 9.82SRR63 pKa = 11.84GLL65 pKa = 3.39

Molecular weight: 8.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

3209

47

387

139.5

16.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.298 ± 0.412

0.717 ± 0.128

5.827 ± 0.487

8.663 ± 0.657

4.238 ± 0.435

4.425 ± 0.405

1.87 ± 0.246

7.043 ± 0.603

10.252 ± 0.555

9.723 ± 0.524

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.87 ± 0.103

6.295 ± 0.444

2.462 ± 0.285

4.706 ± 0.457

4.519 ± 0.539

5.391 ± 0.364

5.921 ± 0.472

5.266 ± 0.269

0.873 ± 0.123

4.643 ± 0.397
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski