Amino acid dipepetide frequency for Rhizobium sp. RU20A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.006AlaAla: 18.006 ± 0.193	1.015AlaCys: 1.015 ± 0.028	7.408AlaAsp: 7.408 ± 0.09	7.995AlaGlu: 7.995 ± 0.116	4.739AlaPhe: 4.739 ± 0.068	11.198AlaGly: 11.198 ± 0.109	2.243AlaHis: 2.243 ± 0.047	6.972AlaIle: 6.972 ± 0.083	3.979AlaLys: 3.979 ± 0.07	13.596AlaLeu: 13.596 ± 0.146	3.685AlaMet: 3.685 ± 0.06	2.871AlaAsn: 2.871 ± 0.048	5.304AlaPro: 5.304 ± 0.073	3.395AlaGln: 3.395 ± 0.062	8.889AlaArg: 8.889 ± 0.093	6.536AlaSer: 6.536 ± 0.076	6.481AlaThr: 6.481 ± 0.07	8.863AlaVal: 8.863 ± 0.096	1.286AlaTrp: 1.286 ± 0.031	2.634AlaTyr: 2.634 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.87CysAla: 0.87 ± 0.027	0.098CysCys: 0.098 ± 0.009	0.492CysAsp: 0.492 ± 0.022	0.427CysGlu: 0.427 ± 0.019	0.311CysPhe: 0.311 ± 0.016	0.887CysGly: 0.887 ± 0.028	0.246CysHis: 0.246 ± 0.014	0.378CysIle: 0.378 ± 0.018	0.176CysLys: 0.176 ± 0.011	0.806CysLeu: 0.806 ± 0.022	0.157CysMet: 0.157 ± 0.012	0.193CysAsn: 0.193 ± 0.013	0.392CysPro: 0.392 ± 0.017	0.219CysGln: 0.219 ± 0.014	0.593CysArg: 0.593 ± 0.022	0.384CysSer: 0.384 ± 0.018	0.387CysThr: 0.387 ± 0.018	0.532CysVal: 0.532 ± 0.018	0.098CysTrp: 0.098 ± 0.008	0.184CysTyr: 0.184 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.95AspAla: 6.95 ± 0.086	0.522AspCys: 0.522 ± 0.02	2.992AspAsp: 2.992 ± 0.056	3.343AspGlu: 3.343 ± 0.06	2.266AspPhe: 2.266 ± 0.04	5.283AspGly: 5.283 ± 0.062	1.292AspHis: 1.292 ± 0.041	3.379AspIle: 3.379 ± 0.049	1.778AspLys: 1.778 ± 0.038	6.024AspLeu: 6.024 ± 0.078	1.461AspMet: 1.461 ± 0.038	1.393AspAsn: 1.393 ± 0.033	3.382AspPro: 3.382 ± 0.047	1.671AspGln: 1.671 ± 0.04	4.449AspArg: 4.449 ± 0.063	2.063AspSer: 2.063 ± 0.042	2.777AspThr: 2.777 ± 0.055	4.165AspVal: 4.165 ± 0.057	0.937AspTrp: 0.937 ± 0.027	1.499AspTyr: 1.499 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.104GluAla: 8.104 ± 0.084	0.331GluCys: 0.331 ± 0.015	2.882GluAsp: 2.882 ± 0.049	3.436GluGlu: 3.436 ± 0.065	1.647GluPhe: 1.647 ± 0.036	4.58GluGly: 4.58 ± 0.064	1.109GluHis: 1.109 ± 0.031	3.693GluIle: 3.693 ± 0.059	2.626GluLys: 2.626 ± 0.049	4.659GluLeu: 4.659 ± 0.063	1.591GluMet: 1.591 ± 0.036	1.711GluAsn: 1.711 ± 0.037	2.646GluPro: 2.646 ± 0.053	1.981GluGln: 1.981 ± 0.046	5.021GluArg: 5.021 ± 0.08	2.332GluSer: 2.332 ± 0.046	4.18GluThr: 4.18 ± 0.066	3.566GluVal: 3.566 ± 0.052	0.582GluTrp: 0.582 ± 0.02	0.883GluTyr: 0.883 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.673PheAla: 4.673 ± 0.07	0.366PheCys: 0.366 ± 0.016	2.746PheAsp: 2.746 ± 0.049	2.221PheGlu: 2.221 ± 0.047	1.541PhePhe: 1.541 ± 0.043	3.672PheGly: 3.672 ± 0.05	0.74PheHis: 0.74 ± 0.026	1.865PheIle: 1.865 ± 0.043	1.043PheLys: 1.043 ± 0.031	3.595PheLeu: 3.595 ± 0.067	0.849PheMet: 0.849 ± 0.024	1.073PheAsn: 1.073 ± 0.032	1.574PhePro: 1.574 ± 0.035	1.055PheGln: 1.055 ± 0.031	2.298PheArg: 2.298 ± 0.038	2.398PheSer: 2.398 ± 0.046	2.007PheThr: 2.007 ± 0.044	2.854PheVal: 2.854 ± 0.052	0.534PheTrp: 0.534 ± 0.02	0.934PheTyr: 0.934 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.274GlyAla: 9.274 ± 0.097	0.772GlyCys: 0.772 ± 0.024	4.394GlyAsp: 4.394 ± 0.062	4.947GlyGlu: 4.947 ± 0.075	3.66GlyPhe: 3.66 ± 0.06	7.169GlyGly: 7.169 ± 0.173	1.963GlyHis: 1.963 ± 0.038	4.923GlyIle: 4.923 ± 0.069	3.461GlyLys: 3.461 ± 0.057	8.933GlyLeu: 8.933 ± 0.093	2.323GlyMet: 2.323 ± 0.046	2.196GlyAsn: 2.196 ± 0.053	3.328GlyPro: 3.328 ± 0.052	2.697GlyGln: 2.697 ± 0.046	6.248GlyArg: 6.248 ± 0.078	4.531GlySer: 4.531 ± 0.068	4.751GlyThr: 4.751 ± 0.077	5.967GlyVal: 5.967 ± 0.068	1.197GlyTrp: 1.197 ± 0.033	2.301GlyTyr: 2.301 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.344HisAla: 2.344 ± 0.047	0.211HisCys: 0.211 ± 0.011	1.265HisAsp: 1.265 ± 0.037	0.989HisGlu: 0.989 ± 0.03	0.846HisPhe: 0.846 ± 0.025	1.949HisGly: 1.949 ± 0.046	0.588HisHis: 0.588 ± 0.024	0.943HisIle: 0.943 ± 0.032	0.452HisLys: 0.452 ± 0.018	2.098HisLeu: 2.098 ± 0.038	0.544HisMet: 0.544 ± 0.022	0.413HisAsn: 0.413 ± 0.018	1.288HisPro: 1.288 ± 0.038	0.579HisGln: 0.579 ± 0.021	1.447HisArg: 1.447 ± 0.035	0.879HisSer: 0.879 ± 0.027	0.828HisThr: 0.828 ± 0.026	1.558HisVal: 1.558 ± 0.035	0.271HisTrp: 0.271 ± 0.015	0.536HisTyr: 0.536 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.785IleAla: 7.785 ± 0.091	0.516IleCys: 0.516 ± 0.021	3.995IleAsp: 3.995 ± 0.066	3.725IleGlu: 3.725 ± 0.061	1.801IlePhe: 1.801 ± 0.045	5.328IleGly: 5.328 ± 0.069	0.947IleHis: 0.947 ± 0.024	2.589IleIle: 2.589 ± 0.051	1.536IleLys: 1.536 ± 0.037	4.764IleLeu: 4.764 ± 0.07	1.022IleMet: 1.022 ± 0.029	1.489IleAsn: 1.489 ± 0.033	2.328IlePro: 2.328 ± 0.049	1.203IleGln: 1.203 ± 0.032	3.573IleArg: 3.573 ± 0.053	3.002IleSer: 3.002 ± 0.053	2.833IleThr: 2.833 ± 0.05	4.462IleVal: 4.462 ± 0.062	0.579IleTrp: 0.579 ± 0.022	1.115IleTyr: 1.115 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.947LysAla: 4.947 ± 0.065	0.133LysCys: 0.133 ± 0.009	1.935LysAsp: 1.935 ± 0.046	1.654LysGlu: 1.654 ± 0.038	0.813LysPhe: 0.813 ± 0.023	2.822LysGly: 2.822 ± 0.057	0.582LysHis: 0.582 ± 0.022	1.806LysIle: 1.806 ± 0.04	1.352LysLys: 1.352 ± 0.038	3.214LysLeu: 3.214 ± 0.052	0.817LysMet: 0.817 ± 0.026	0.932LysAsn: 0.932 ± 0.024	2.157LysPro: 2.157 ± 0.048	0.992LysGln: 0.992 ± 0.029	2.498LysArg: 2.498 ± 0.054	1.89LysSer: 1.89 ± 0.043	2.322LysThr: 2.322 ± 0.042	2.506LysVal: 2.506 ± 0.048	0.355LysTrp: 0.355 ± 0.016	0.614LysTyr: 0.614 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.527LeuAla: 13.527 ± 0.139	0.857LeuCys: 0.857 ± 0.025	5.894LeuAsp: 5.894 ± 0.071	5.188LeuGlu: 5.188 ± 0.067	3.7LeuPhe: 3.7 ± 0.067	7.801LeuGly: 7.801 ± 0.093	1.718LeuHis: 1.718 ± 0.042	5.113LeuIle: 5.113 ± 0.077	4.18LeuLys: 4.18 ± 0.066	9.135LeuLeu: 9.135 ± 0.134	2.481LeuMet: 2.481 ± 0.049	2.468LeuAsn: 2.468 ± 0.045	5.419LeuPro: 5.419 ± 0.078	2.617LeuGln: 2.617 ± 0.048	6.092LeuArg: 6.092 ± 0.076	7.089LeuSer: 7.089 ± 0.09	5.889LeuThr: 5.889 ± 0.081	7.505LeuVal: 7.505 ± 0.081	1.046LeuTrp: 1.046 ± 0.03	2.082LeuTyr: 2.082 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.347MetAla: 3.347 ± 0.051	0.133MetCys: 0.133 ± 0.011	1.168MetAsp: 1.168 ± 0.031	1.219MetGlu: 1.219 ± 0.032	0.683MetPhe: 0.683 ± 0.026	1.817MetGly: 1.817 ± 0.041	0.453MetHis: 0.453 ± 0.02	1.495MetIle: 1.495 ± 0.033	1.081MetLys: 1.081 ± 0.032	2.559MetLeu: 2.559 ± 0.048	0.728MetMet: 0.728 ± 0.027	0.777MetAsn: 0.777 ± 0.028	1.502MetPro: 1.502 ± 0.035	0.869MetGln: 0.869 ± 0.026	1.878MetArg: 1.878 ± 0.034	1.644MetSer: 1.644 ± 0.031	2.059MetThr: 2.059 ± 0.039	1.776MetVal: 1.776 ± 0.038	0.189MetTrp: 0.189 ± 0.014	0.277MetTyr: 0.277 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.213AsnAla: 3.213 ± 0.061	0.211AsnCys: 0.211 ± 0.014	1.419AsnAsp: 1.419 ± 0.033	1.319AsnGlu: 1.319 ± 0.035	0.95AsnPhe: 0.95 ± 0.026	2.469AsnGly: 2.469 ± 0.05	0.502AsnHis: 0.502 ± 0.019	1.407AsnIle: 1.407 ± 0.033	0.688AsnLys: 0.688 ± 0.024	2.473AsnLeu: 2.473 ± 0.044	0.613AsnMet: 0.613 ± 0.022	0.685AsnAsn: 0.685 ± 0.028	1.781AsnPro: 1.781 ± 0.039	0.75AsnGln: 0.75 ± 0.026	1.879AsnArg: 1.879 ± 0.038	1.153AsnSer: 1.153 ± 0.032	1.318AsnThr: 1.318 ± 0.031	1.854AsnVal: 1.854 ± 0.038	0.406AsnTrp: 0.406 ± 0.019	0.591AsnTyr: 0.591 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	6.423ProAla: 6.423 ± 0.087	0.31ProCys: 0.31 ± 0.016	3.56ProAsp: 3.56 ± 0.053	3.604ProGlu: 3.604 ± 0.056	2.093ProPhe: 2.093 ± 0.043	4.256ProGly: 4.256 ± 0.052	1.045ProHis: 1.045 ± 0.029	2.27ProIle: 2.27 ± 0.039	1.696ProLys: 1.696 ± 0.041	4.732ProLeu: 4.732 ± 0.065	1.165ProMet: 1.165 ± 0.031	1.177ProAsn: 1.177 ± 0.03	2.168ProPro: 2.168 ± 0.058	1.625ProGln: 1.625 ± 0.039	2.681ProArg: 2.681 ± 0.048	2.612ProSer: 2.612 ± 0.048	2.373ProThr: 2.373 ± 0.045	4.435ProVal: 4.435 ± 0.074	0.56ProTrp: 0.56 ± 0.02	1.134ProTyr: 1.134 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.892GlnAla: 3.892 ± 0.062	0.175GlnCys: 0.175 ± 0.012	1.337GlnAsp: 1.337 ± 0.03	1.439GlnGlu: 1.439 ± 0.038	0.996GlnPhe: 0.996 ± 0.027	2.013GlnGly: 2.013 ± 0.047	0.588GlnHis: 0.588 ± 0.021	1.808GlnIle: 1.808 ± 0.056	1.137GlnLys: 1.137 ± 0.032	2.64GlnLeu: 2.64 ± 0.045	0.958GlnMet: 0.958 ± 0.025	0.811GlnAsn: 0.811 ± 0.026	1.771GlnPro: 1.771 ± 0.039	1.227GlnGln: 1.227 ± 0.047	2.319GlnArg: 2.319 ± 0.045	1.772GlnSer: 1.772 ± 0.042	1.821GlnThr: 1.821 ± 0.044	2.04GlnVal: 2.04 ± 0.052	0.321GlnTrp: 0.321 ± 0.015	0.528GlnTyr: 0.528 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	7.866ArgAla: 7.866 ± 0.096	0.405ArgCys: 0.405 ± 0.02	4.08ArgAsp: 4.08 ± 0.067	3.984ArgGlu: 3.984 ± 0.057	3.061ArgPhe: 3.061 ± 0.053	4.617ArgGly: 4.617 ± 0.058	1.736ArgHis: 1.736 ± 0.036	4.082ArgIle: 4.082 ± 0.056	2.434ArgLys: 2.434 ± 0.056	7.841ArgLeu: 7.841 ± 0.096	1.935ArgMet: 1.935 ± 0.047	1.818ArgAsn: 1.818 ± 0.036	3.625ArgPro: 3.625 ± 0.06	2.671ArgGln: 2.671 ± 0.046	5.669ArgArg: 5.669 ± 0.079	3.624ArgSer: 3.624 ± 0.059	3.426ArgThr: 3.426 ± 0.055	4.439ArgVal: 4.439 ± 0.061	0.83ArgTrp: 0.83 ± 0.026	1.696ArgTyr: 1.696 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.361SerAla: 6.361 ± 0.089	0.373SerCys: 0.373 ± 0.017	3.049SerAsp: 3.049 ± 0.054	2.962SerGlu: 2.962 ± 0.05	2.344SerPhe: 2.344 ± 0.043	5.548SerGly: 5.548 ± 0.084	1.101SerHis: 1.101 ± 0.027	2.906SerIle: 2.906 ± 0.059	1.629SerLys: 1.629 ± 0.044	5.59SerLeu: 5.59 ± 0.074	1.361SerMet: 1.361 ± 0.033	1.348SerAsn: 1.348 ± 0.036	2.657SerPro: 2.657 ± 0.047	1.553SerGln: 1.553 ± 0.043	3.628SerArg: 3.628 ± 0.062	2.961SerSer: 2.961 ± 0.061	2.808SerThr: 2.808 ± 0.061	4.132SerVal: 4.132 ± 0.06	0.683SerTrp: 0.683 ± 0.028	1.237SerTyr: 1.237 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.937ThrAla: 6.937 ± 0.086	0.438ThrCys: 0.438 ± 0.022	2.992ThrAsp: 2.992 ± 0.052	2.791ThrGlu: 2.791 ± 0.042	2.222ThrPhe: 2.222 ± 0.043	5.425ThrGly: 5.425 ± 0.07	1.066ThrHis: 1.066 ± 0.028	3.205ThrIle: 3.205 ± 0.055	1.517ThrLys: 1.517 ± 0.038	6.153ThrLeu: 6.153 ± 0.08	1.297ThrMet: 1.297 ± 0.032	1.316ThrAsn: 1.316 ± 0.035	3.278ThrPro: 3.278 ± 0.062	1.377ThrGln: 1.377 ± 0.031	3.333ThrArg: 3.333 ± 0.06	2.877ThrSer: 2.877 ± 0.057	3.085ThrThr: 3.085 ± 0.062	4.77ThrVal: 4.77 ± 0.059	0.59ThrTrp: 0.59 ± 0.022	1.256ThrTyr: 1.256 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	9.105ValAla: 9.105 ± 0.102	0.616ValCys: 0.616 ± 0.023	4.043ValAsp: 4.043 ± 0.057	4.591ValGlu: 4.591 ± 0.062	2.915ValPhe: 2.915 ± 0.048	5.386ValGly: 5.386 ± 0.065	1.352ValHis: 1.352 ± 0.035	4.25ValIle: 4.25 ± 0.056	2.475ValLys: 2.475 ± 0.047	7.312ValLeu: 7.312 ± 0.09	1.842ValMet: 1.842 ± 0.042	1.98ValAsn: 1.98 ± 0.039	3.629ValPro: 3.629 ± 0.056	1.841ValGln: 1.841 ± 0.039	4.616ValArg: 4.616 ± 0.067	4.602ValSer: 4.602 ± 0.06	4.807ValThr: 4.807 ± 0.07	5.787ValVal: 5.787 ± 0.084	0.84ValTrp: 0.84 ± 0.026	1.499ValTyr: 1.499 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.103TrpAla: 1.103 ± 0.033	0.115TrpCys: 0.115 ± 0.01	0.569TrpAsp: 0.569 ± 0.02	0.506TrpGlu: 0.506 ± 0.021	0.509TrpPhe: 0.509 ± 0.022	0.755TrpGly: 0.755 ± 0.026	0.288TrpHis: 0.288 ± 0.014	0.596TrpIle: 0.596 ± 0.022	0.467TrpLys: 0.467 ± 0.021	1.458TrpLeu: 1.458 ± 0.037	0.346TrpMet: 0.346 ± 0.016	0.417TrpAsn: 0.417 ± 0.019	0.621TrpPro: 0.621 ± 0.023	0.523TrpGln: 0.523 ± 0.02	0.941TrpArg: 0.941 ± 0.027	0.734TrpSer: 0.734 ± 0.029	0.688TrpThr: 0.688 ± 0.023	0.683TrpVal: 0.683 ± 0.022	0.204TrpTrp: 0.204 ± 0.014	0.303TrpTyr: 0.303 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.371TyrAla: 2.371 ± 0.045	0.233TyrCys: 0.233 ± 0.015	1.405TyrAsp: 1.405 ± 0.032	1.222TyrGlu: 1.222 ± 0.029	0.939TyrPhe: 0.939 ± 0.026	2.059TyrGly: 2.059 ± 0.039	0.47TyrHis: 0.47 ± 0.018	0.912TyrIle: 0.912 ± 0.025	0.635TyrLys: 0.635 ± 0.024	2.219TyrLeu: 2.219 ± 0.046	0.476TyrMet: 0.476 ± 0.018	0.612TyrAsn: 0.612 ± 0.027	1.067TyrPro: 1.067 ± 0.029	0.752TyrGln: 0.752 ± 0.023	1.786TyrArg: 1.786 ± 0.046	1.147TyrSer: 1.147 ± 0.031	1.087TyrThr: 1.087 ± 0.033	1.592TyrVal: 1.592 ± 0.039	0.317TyrTrp: 0.317 ± 0.017	0.599TyrTyr: 0.599 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4181 proteins (1320648 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---