Torilis crimson leaf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2MW64|A0A6H2MW64_9LUTE Readthrough protein OS=Torilis crimson leaf virus OX=1862685 PE=3 SV=1
MM1 pKa = 7.21QLEE4 pKa = 4.29IQRR7 pKa = 11.84SGIVTFTARR16 pKa = 11.84SFFSRR21 pKa = 11.84LFAYY25 pKa = 9.91RR26 pKa = 11.84YY27 pKa = 9.61YY28 pKa = 9.28ITHH31 pKa = 7.61ALTLSRR37 pKa = 11.84QLNNVEE43 pKa = 3.95PSFPEE48 pKa = 3.74TLPRR52 pKa = 11.84SLLFSLPFIINNNYY66 pKa = 10.85DD67 pKa = 3.14MGDD70 pKa = 3.35GYY72 pKa = 10.77IRR74 pKa = 11.84FQPDD78 pKa = 2.88HH79 pKa = 6.78LRR81 pKa = 11.84EE82 pKa = 3.88FVLWGLICGYY92 pKa = 9.07YY93 pKa = 9.76PRR95 pKa = 11.84LNKK98 pKa = 9.93RR99 pKa = 11.84GRR101 pKa = 11.84RR102 pKa = 11.84GYY104 pKa = 10.34RR105 pKa = 11.84ATLAARR111 pKa = 11.84VEE113 pKa = 4.0RR114 pKa = 11.84RR115 pKa = 11.84AYY117 pKa = 10.86LSTLHH122 pKa = 6.49NLDD125 pKa = 2.99WRR127 pKa = 11.84NLEE130 pKa = 3.86EE131 pKa = 4.39NMFRR135 pKa = 11.84RR136 pKa = 11.84QEE138 pKa = 4.15CFFQSAEE145 pKa = 4.04GFSRR149 pKa = 11.84MLGGLFSIWNRR160 pKa = 11.84DD161 pKa = 2.85AANRR165 pKa = 11.84YY166 pKa = 9.71GEE168 pKa = 4.69LPLDD172 pKa = 3.7ACRR175 pKa = 11.84NLVDD179 pKa = 4.79SIFVGSKK186 pKa = 10.37VYY188 pKa = 10.12MDD190 pKa = 5.07LSDD193 pKa = 5.22LFHH196 pKa = 7.11DD197 pKa = 4.21TCPCIGYY204 pKa = 8.92SHH206 pKa = 7.3LNNNGDD212 pKa = 4.07LPCGQMDD219 pKa = 5.78LFTAPYY225 pKa = 10.91ALYY228 pKa = 9.6TLSADD233 pKa = 3.22HH234 pKa = 6.82HH235 pKa = 6.85KK236 pKa = 9.95IHH238 pKa = 6.64HH239 pKa = 6.17WNAGTSEE246 pKa = 4.27EE247 pKa = 4.25NSDD250 pKa = 4.59SEE252 pKa = 5.36GEE254 pKa = 4.29GLL256 pKa = 4.7

Molecular weight: 29.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2MW59|A0A6H2MW59_9LUTE P0 OS=Torilis crimson leaf virus OX=1862685 PE=4 SV=1
MM1 pKa = 7.98PIYY4 pKa = 10.82GLTKK8 pKa = 10.05RR9 pKa = 11.84STGNKK14 pKa = 8.7PMVVVNSPPRR24 pKa = 11.84RR25 pKa = 11.84PRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84VRR31 pKa = 11.84TKK33 pKa = 10.3PIVVVQTTQPGRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84GRR53 pKa = 11.84NGRR56 pKa = 11.84GPRR59 pKa = 11.84TMGQSRR65 pKa = 11.84GGANRR70 pKa = 11.84EE71 pKa = 3.63QLVFSKK77 pKa = 11.03DD78 pKa = 3.69DD79 pKa = 3.42IKK81 pKa = 11.46GNSYY85 pKa = 10.83GAITFGKK92 pKa = 10.08DD93 pKa = 3.64LSDD96 pKa = 3.86HH97 pKa = 6.77PAFSSGILKK106 pKa = 10.4AFHH109 pKa = 6.93EE110 pKa = 4.46YY111 pKa = 10.6KK112 pKa = 9.35ITNLRR117 pKa = 11.84VIFKK121 pKa = 10.49SEE123 pKa = 3.96APSTTGGSLAYY134 pKa = 9.74EE135 pKa = 4.61LDD137 pKa = 3.63PHH139 pKa = 7.38CEE141 pKa = 4.02LTSLSSKK148 pKa = 9.75IYY150 pKa = 10.65KK151 pKa = 10.54FGATKK156 pKa = 10.42GGQKK160 pKa = 8.26TWTAKK165 pKa = 10.18EE166 pKa = 3.82INGTEE171 pKa = 3.79WHH173 pKa = 6.71SSSEE177 pKa = 3.96NQFRR181 pKa = 11.84LLYY184 pKa = 10.08KK185 pKa = 10.91GNGDD189 pKa = 3.48STQIGSFEE197 pKa = 3.51IHH199 pKa = 5.26YY200 pKa = 8.84TVLFQNPKK208 pKa = 9.88

Molecular weight: 23.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3123

92

1051

446.1

49.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.116 ± 0.47

1.569 ± 0.254

4.131 ± 0.529

6.084 ± 0.356

4.387 ± 0.365

6.948 ± 0.556

1.921 ± 0.209

5.027 ± 0.391

5.828 ± 0.694

9.03 ± 0.541

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.985 ± 0.161

5.379 ± 0.28

5.476 ± 0.343

3.746 ± 0.424

6.308 ± 0.798

8.774 ± 0.459

7.077 ± 0.527

5.572 ± 0.499

1.697 ± 0.179

2.914 ± 0.494
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski