Exophiala dermatitidis (strain ATCC 34100 / CBS 525.76 / NIH/UT8656) (Black yeast) (Wangiella dermatitidis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Exophiala; Exophiala dermatitidis

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9391 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6C5G2|H6C5G2_EXODN Uncharacterized protein OS=Exophiala dermatitidis (strain ATCC 34100 / CBS 525.76 / NIH/UT8656) OX=858893 GN=HMPREF1120_07802 PE=4 SV=1
MM1 pKa = 7.24AASRR5 pKa = 11.84SGLVSASAFISPAALLIEE23 pKa = 4.7KK24 pKa = 9.57DD25 pKa = 3.47GVMIATGVLACFCGCVAGVGRR46 pKa = 11.84EE47 pKa = 4.0FVLGAEE53 pKa = 4.71DD54 pKa = 4.85GVTCVGSDD62 pKa = 3.02HH63 pKa = 7.18LHH65 pKa = 6.73FCNCDD70 pKa = 3.37DD71 pKa = 5.38NNNDD75 pKa = 4.29DD76 pKa = 5.6HH77 pKa = 8.75DD78 pKa = 6.19DD79 pKa = 4.01DD80 pKa = 6.81DD81 pKa = 4.72EE82 pKa = 6.79GGARR86 pKa = 11.84GDD88 pKa = 3.5HH89 pKa = 6.64AEE91 pKa = 4.24DD92 pKa = 4.44DD93 pKa = 4.38GVVVVVTTT101 pKa = 4.14

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6CAR1|H6CAR1_EXODN Uncharacterized protein OS=Exophiala dermatitidis (strain ATCC 34100 / CBS 525.76 / NIH/UT8656) OX=858893 GN=HMPREF1120_08802 PE=4 SV=1
VV1 pKa = 7.18LNTVRR6 pKa = 11.84TSSTRR11 pKa = 11.84KK12 pKa = 7.85KK13 pKa = 8.16TARR16 pKa = 11.84PQHH19 pKa = 6.19FSTSARR25 pKa = 11.84PATRR29 pKa = 11.84PPPTTLHH36 pKa = 5.66AHH38 pKa = 6.32TGISWVPPFRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84LEE52 pKa = 4.25SPLMSQMIQLFLMQLNGVRR71 pKa = 11.84SVGKK75 pKa = 9.85RR76 pKa = 11.84MRR78 pKa = 11.84FISSPNSGLRR88 pKa = 11.84TRR90 pKa = 11.84GG91 pKa = 3.16

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9285

106

9391

4712771

51

5812

501.8

55.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.786 ± 0.021

1.156 ± 0.009

5.72 ± 0.018

6.17 ± 0.026

3.517 ± 0.015

6.872 ± 0.021

2.457 ± 0.01

4.617 ± 0.017

4.844 ± 0.025

8.738 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.01

3.61 ± 0.011

6.237 ± 0.021

4.265 ± 0.018

6.285 ± 0.02

8.322 ± 0.032

6.158 ± 0.029

6.107 ± 0.018

1.373 ± 0.01

2.682 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski