Amino acid dipepetide frequency for Gossypium raimondii (New World cotton)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.317AlaAla: 6.317 ± 0.021	1.25AlaCys: 1.25 ± 0.007	3.155AlaAsp: 3.155 ± 0.011	4.267AlaGlu: 4.267 ± 0.017	2.762AlaPhe: 2.762 ± 0.011	3.989AlaGly: 3.989 ± 0.016	1.233AlaHis: 1.233 ± 0.007	3.799AlaIle: 3.799 ± 0.013	3.964AlaLys: 3.964 ± 0.013	6.609AlaLeu: 6.609 ± 0.019	1.777AlaMet: 1.777 ± 0.009	2.645AlaAsn: 2.645 ± 0.012	2.826AlaPro: 2.826 ± 0.012	2.105AlaGln: 2.105 ± 0.011	3.231AlaArg: 3.231 ± 0.011	6.099AlaSer: 6.099 ± 0.018	3.63AlaThr: 3.63 ± 0.012	4.814AlaVal: 4.814 ± 0.016	0.737AlaTrp: 0.737 ± 0.005	1.799AlaTyr: 1.799 ± 0.008	0.0AlaXaa: 0.0 ± 0.0
Cys	0.959CysAla: 0.959 ± 0.007	0.554CysCys: 0.554 ± 0.005	0.872CysAsp: 0.872 ± 0.007	0.892CysGlu: 0.892 ± 0.006	0.948CysPhe: 0.948 ± 0.006	1.335CysGly: 1.335 ± 0.009	0.468CysHis: 0.468 ± 0.004	1.035CysIle: 1.035 ± 0.007	1.171CysLys: 1.171 ± 0.009	1.962CysLeu: 1.962 ± 0.01	0.459CysMet: 0.459 ± 0.004	0.9CysAsn: 0.9 ± 0.006	0.915CysPro: 0.915 ± 0.006	0.619CysGln: 0.619 ± 0.006	1.03CysArg: 1.03 ± 0.007	1.861CysSer: 1.861 ± 0.009	0.824CysThr: 0.824 ± 0.006	1.013CysVal: 1.013 ± 0.006	0.261CysTrp: 0.261 ± 0.003	0.567CysTyr: 0.567 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	3.48AspAla: 3.48 ± 0.014	0.974AspCys: 0.974 ± 0.006	3.659AspAsp: 3.659 ± 0.017	4.027AspGlu: 4.027 ± 0.015	2.319AspPhe: 2.319 ± 0.009	3.787AspGly: 3.787 ± 0.014	1.205AspHis: 1.205 ± 0.007	3.064AspIle: 3.064 ± 0.01	2.841AspLys: 2.841 ± 0.014	5.05AspLeu: 5.05 ± 0.017	1.299AspMet: 1.299 ± 0.007	2.17AspAsn: 2.17 ± 0.009	2.61AspPro: 2.61 ± 0.01	1.741AspGln: 1.741 ± 0.008	2.329AspArg: 2.329 ± 0.011	4.229AspSer: 4.229 ± 0.013	2.168AspThr: 2.168 ± 0.009	3.596AspVal: 3.596 ± 0.013	0.698AspTrp: 0.698 ± 0.006	1.541AspTyr: 1.541 ± 0.008	0.0AspXaa: 0.0 ± 0.0
Glu	4.804GluAla: 4.804 ± 0.019	0.92GluCys: 0.92 ± 0.006	3.972GluAsp: 3.972 ± 0.018	6.17GluGlu: 6.17 ± 0.035	2.386GluPhe: 2.386 ± 0.01	3.668GluGly: 3.668 ± 0.013	1.267GluHis: 1.267 ± 0.007	3.749GluIle: 3.749 ± 0.015	4.937GluLys: 4.937 ± 0.024	6.025GluLeu: 6.025 ± 0.019	1.768GluMet: 1.768 ± 0.008	3.284GluAsn: 3.284 ± 0.015	2.19GluPro: 2.19 ± 0.011	2.265GluGln: 2.265 ± 0.01	3.403GluArg: 3.403 ± 0.015	4.651GluSer: 4.651 ± 0.019	3.272GluThr: 3.272 ± 0.02	4.19GluVal: 4.19 ± 0.016	0.721GluTrp: 0.721 ± 0.006	1.654GluTyr: 1.654 ± 0.007	0.0GluXaa: 0.0 ± 0.0
Phe	2.482PheAla: 2.482 ± 0.01	0.922PheCys: 0.922 ± 0.006	2.335PheAsp: 2.335 ± 0.009	2.337PheGlu: 2.337 ± 0.009	2.04PhePhe: 2.04 ± 0.01	3.064PheGly: 3.064 ± 0.016	1.133PheHis: 1.133 ± 0.007	2.169PheIle: 2.169 ± 0.011	2.187PheLys: 2.187 ± 0.011	4.343PheLeu: 4.343 ± 0.016	1.009PheMet: 1.009 ± 0.007	1.863PheAsn: 1.863 ± 0.009	2.152PhePro: 2.152 ± 0.01	1.615PheGln: 1.615 ± 0.008	2.019PheArg: 2.019 ± 0.008	4.173PheSer: 4.173 ± 0.013	1.99PheThr: 1.99 ± 0.01	2.684PheVal: 2.684 ± 0.012	0.578PheTrp: 0.578 ± 0.005	1.306PheTyr: 1.306 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	3.804GlyAla: 3.804 ± 0.015	1.276GlyCys: 1.276 ± 0.007	3.364GlyAsp: 3.364 ± 0.012	3.601GlyGlu: 3.601 ± 0.014	3.136GlyPhe: 3.136 ± 0.012	5.137GlyGly: 5.137 ± 0.026	1.522GlyHis: 1.522 ± 0.008	3.66GlyIle: 3.66 ± 0.011	4.187GlyLys: 4.187 ± 0.012	5.831GlyLeu: 5.831 ± 0.017	1.503GlyMet: 1.503 ± 0.008	3.289GlyAsn: 3.289 ± 0.014	2.425GlyPro: 2.425 ± 0.011	2.108GlyGln: 2.108 ± 0.011	3.427GlyArg: 3.427 ± 0.015	6.176GlySer: 6.176 ± 0.019	3.318GlyThr: 3.318 ± 0.012	4.09GlyVal: 4.09 ± 0.012	0.879GlyTrp: 0.879 ± 0.006	2.054GlyTyr: 2.054 ± 0.01	0.0GlyXaa: 0.0 ± 0.0
His	1.381HisAla: 1.381 ± 0.007	0.536HisCys: 0.536 ± 0.005	1.104HisAsp: 1.104 ± 0.007	1.293HisGlu: 1.293 ± 0.008	1.112HisPhe: 1.112 ± 0.006	1.697HisGly: 1.697 ± 0.008	0.968HisHis: 0.968 ± 0.01	1.205HisIle: 1.205 ± 0.007	1.172HisLys: 1.172 ± 0.007	2.428HisLeu: 2.428 ± 0.01	0.526HisMet: 0.526 ± 0.004	0.95HisAsn: 0.95 ± 0.007	1.406HisPro: 1.406 ± 0.007	1.036HisGln: 1.036 ± 0.007	1.343HisArg: 1.343 ± 0.008	1.943HisSer: 1.943 ± 0.009	0.924HisThr: 0.924 ± 0.006	1.506HisVal: 1.506 ± 0.008	0.307HisTrp: 0.307 ± 0.003	0.714HisTyr: 0.714 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	3.586IleAla: 3.586 ± 0.012	1.163IleCys: 1.163 ± 0.007	2.927IleAsp: 2.927 ± 0.011	3.324IleGlu: 3.324 ± 0.013	2.336IlePhe: 2.336 ± 0.01	3.393IleGly: 3.393 ± 0.013	1.279IleHis: 1.279 ± 0.006	2.845IleIle: 2.845 ± 0.01	3.025IleLys: 3.025 ± 0.011	5.204IleLeu: 5.204 ± 0.017	1.151IleMet: 1.151 ± 0.007	2.306IleAsn: 2.306 ± 0.011	2.966IlePro: 2.966 ± 0.011	1.994IleGln: 1.994 ± 0.009	2.618IleArg: 2.618 ± 0.01	4.936IleSer: 4.936 ± 0.014	2.58IleThr: 2.58 ± 0.01	3.452IleVal: 3.452 ± 0.011	0.702IleTrp: 0.702 ± 0.007	1.498IleTyr: 1.498 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	4.233LysAla: 4.233 ± 0.015	0.979LysCys: 0.979 ± 0.006	3.356LysAsp: 3.356 ± 0.015	4.868LysGlu: 4.868 ± 0.024	2.207LysPhe: 2.207 ± 0.009	3.766LysGly: 3.766 ± 0.013	1.418LysHis: 1.418 ± 0.007	3.248LysIle: 3.248 ± 0.011	4.801LysLys: 4.801 ± 0.023	6.14LysLeu: 6.14 ± 0.018	1.519LysMet: 1.519 ± 0.007	2.717LysAsn: 2.717 ± 0.01	2.916LysPro: 2.916 ± 0.012	2.497LysGln: 2.497 ± 0.011	3.599LysArg: 3.599 ± 0.012	4.715LysSer: 4.715 ± 0.016	2.979LysThr: 2.979 ± 0.011	3.976LysVal: 3.976 ± 0.015	0.781LysTrp: 0.781 ± 0.005	1.653LysTyr: 1.653 ± 0.008	0.0LysXaa: 0.0 ± 0.0
Leu	6.445LeuAla: 6.445 ± 0.02	1.833LeuCys: 1.833 ± 0.01	5.082LeuAsp: 5.082 ± 0.015	6.441LeuGlu: 6.441 ± 0.02	3.918LeuPhe: 3.918 ± 0.017	5.736LeuGly: 5.736 ± 0.018	2.577LeuHis: 2.577 ± 0.01	4.613LeuIle: 4.613 ± 0.013	6.424LeuLys: 6.424 ± 0.018	9.939LeuLeu: 9.939 ± 0.029	2.185LeuMet: 2.185 ± 0.01	4.149LeuAsn: 4.149 ± 0.013	5.172LeuPro: 5.172 ± 0.019	4.48LeuGln: 4.48 ± 0.015	5.304LeuArg: 5.304 ± 0.015	8.708LeuSer: 8.708 ± 0.026	4.499LeuThr: 4.499 ± 0.016	6.242LeuVal: 6.242 ± 0.017	1.138LeuTrp: 1.138 ± 0.007	2.542LeuTyr: 2.542 ± 0.01	0.0LeuXaa: 0.0 ± 0.0
Met	2.132MetAla: 2.132 ± 0.011	0.312MetCys: 0.312 ± 0.003	1.45MetAsp: 1.45 ± 0.007	2.028MetGlu: 2.028 ± 0.009	0.842MetPhe: 0.842 ± 0.006	1.611MetGly: 1.611 ± 0.008	0.541MetHis: 0.541 ± 0.004	1.177MetIle: 1.177 ± 0.007	1.664MetLys: 1.664 ± 0.009	2.233MetLeu: 2.233 ± 0.01	0.679MetMet: 0.679 ± 0.005	1.042MetAsn: 1.042 ± 0.006	1.059MetPro: 1.059 ± 0.007	0.983MetGln: 0.983 ± 0.007	1.151MetArg: 1.151 ± 0.007	1.773MetSer: 1.773 ± 0.008	1.026MetThr: 1.026 ± 0.006	1.7MetVal: 1.7 ± 0.008	0.243MetTrp: 0.243 ± 0.003	0.56MetTyr: 0.56 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.761AsnAla: 2.761 ± 0.012	0.886AsnCys: 0.886 ± 0.006	2.177AsnAsp: 2.177 ± 0.01	2.732AsnGlu: 2.732 ± 0.013	1.938AsnPhe: 1.938 ± 0.008	3.48AsnGly: 3.48 ± 0.013	1.136AsnHis: 1.136 ± 0.006	2.532AsnIle: 2.532 ± 0.01	2.537AsnLys: 2.537 ± 0.01	4.747AsnLeu: 4.747 ± 0.022	1.139AsnMet: 1.139 ± 0.007	2.405AsnAsn: 2.405 ± 0.013	2.432AsnPro: 2.432 ± 0.01	1.839AsnGln: 1.839 ± 0.009	2.052AsnArg: 2.052 ± 0.01	3.974AsnSer: 3.974 ± 0.015	1.958AsnThr: 1.958 ± 0.008	2.845AsnVal: 2.845 ± 0.01	0.568AsnTrp: 0.568 ± 0.004	1.298AsnTyr: 1.298 ± 0.008	0.0AsnXaa: 0.0 ± 0.0
Pro	2.992ProAla: 2.992 ± 0.013	0.8ProCys: 0.8 ± 0.006	2.434ProAsp: 2.434 ± 0.01	3.124ProGlu: 3.124 ± 0.013	2.1ProPhe: 2.1 ± 0.011	2.693ProGly: 2.693 ± 0.012	1.086ProHis: 1.086 ± 0.007	2.341ProIle: 2.341 ± 0.01	2.872ProLys: 2.872 ± 0.012	4.447ProLeu: 4.447 ± 0.016	1.014ProMet: 1.014 ± 0.007	2.327ProAsn: 2.327 ± 0.011	3.812ProPro: 3.812 ± 0.037	1.885ProGln: 1.885 ± 0.011	2.329ProArg: 2.329 ± 0.011	5.231ProSer: 5.231 ± 0.02	2.642ProThr: 2.642 ± 0.012	3.152ProVal: 3.152 ± 0.011	0.592ProTrp: 0.592 ± 0.005	1.327ProTyr: 1.327 ± 0.009	0.0ProXaa: 0.0 ± 0.0
Gln	2.494GlnAla: 2.494 ± 0.01	0.601GlnCys: 0.601 ± 0.005	1.666GlnAsp: 1.666 ± 0.008	2.4GlnGlu: 2.4 ± 0.011	1.43GlnPhe: 1.43 ± 0.008	2.183GlnGly: 2.183 ± 0.01	0.984GlnHis: 0.984 ± 0.007	2.084GlnIle: 2.084 ± 0.01	2.37GlnLys: 2.37 ± 0.012	3.795GlnLeu: 3.795 ± 0.014	1.003GlnMet: 1.003 ± 0.008	1.851GlnAsn: 1.851 ± 0.009	1.84GlnPro: 1.84 ± 0.011	2.333GlnGln: 2.333 ± 0.028	2.136GlnArg: 2.136 ± 0.009	3.018GlnSer: 3.018 ± 0.014	1.76GlnThr: 1.76 ± 0.009	2.425GlnVal: 2.425 ± 0.01	0.47GlnTrp: 0.47 ± 0.004	0.967GlnTyr: 0.967 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.109ArgAla: 3.109 ± 0.012	0.961ArgCys: 0.961 ± 0.007	2.578ArgAsp: 2.578 ± 0.012	3.279ArgGlu: 3.279 ± 0.015	2.209ArgPhe: 2.209 ± 0.009	3.048ArgGly: 3.048 ± 0.013	1.251ArgHis: 1.251 ± 0.006	2.821ArgIle: 2.821 ± 0.011	3.793ArgLys: 3.793 ± 0.015	4.965ArgLeu: 4.965 ± 0.015	1.278ArgMet: 1.278 ± 0.007	2.476ArgAsn: 2.476 ± 0.01	2.25ArgPro: 2.25 ± 0.009	1.88ArgGln: 1.88 ± 0.009	3.696ArgArg: 3.696 ± 0.015	4.289ArgSer: 4.289 ± 0.016	2.424ArgThr: 2.424 ± 0.01	3.153ArgVal: 3.153 ± 0.013	0.695ArgTrp: 0.695 ± 0.005	1.468ArgTyr: 1.468 ± 0.008	0.0ArgXaa: 0.0 ± 0.0
Ser	5.393SerAla: 5.393 ± 0.016	1.728SerCys: 1.728 ± 0.009	4.432SerAsp: 4.432 ± 0.013	4.928SerGlu: 4.928 ± 0.018	4.123SerPhe: 4.123 ± 0.014	5.949SerGly: 5.949 ± 0.019	1.986SerHis: 1.986 ± 0.009	4.615SerIle: 4.615 ± 0.014	5.234SerLys: 5.234 ± 0.017	8.816SerLeu: 8.816 ± 0.024	2.142SerMet: 2.142 ± 0.009	4.28SerAsn: 4.28 ± 0.015	4.613SerPro: 4.613 ± 0.022	3.138SerGln: 3.138 ± 0.013	4.399SerArg: 4.399 ± 0.014	11.27SerSer: 11.27 ± 0.035	4.788SerThr: 4.788 ± 0.016	5.196SerVal: 5.196 ± 0.015	1.125SerTrp: 1.125 ± 0.007	2.323SerTyr: 2.323 ± 0.009	0.0SerXaa: 0.0 ± 0.0
Thr	3.465ThrAla: 3.465 ± 0.012	0.936ThrCys: 0.936 ± 0.005	2.324ThrAsp: 2.324 ± 0.009	2.833ThrGlu: 2.833 ± 0.013	2.033ThrPhe: 2.033 ± 0.01	3.306ThrGly: 3.306 ± 0.012	0.988ThrHis: 0.988 ± 0.006	2.757ThrIle: 2.757 ± 0.012	2.771ThrLys: 2.771 ± 0.012	4.655ThrLeu: 4.655 ± 0.012	1.183ThrMet: 1.183 ± 0.006	2.112ThrAsn: 2.112 ± 0.009	2.595ThrPro: 2.595 ± 0.011	1.549ThrGln: 1.549 ± 0.008	2.302ThrArg: 2.302 ± 0.009	4.676ThrSer: 4.676 ± 0.016	2.909ThrThr: 2.909 ± 0.013	3.395ThrVal: 3.395 ± 0.011	0.605ThrTrp: 0.605 ± 0.006	1.339ThrTyr: 1.339 ± 0.007	0.0ThrXaa: 0.0 ± 0.0
Val	4.732ValAla: 4.732 ± 0.015	1.143ValCys: 1.143 ± 0.007	3.693ValAsp: 3.693 ± 0.014	4.399ValGlu: 4.399 ± 0.017	2.693ValPhe: 2.693 ± 0.012	4.091ValGly: 4.091 ± 0.014	1.488ValHis: 1.488 ± 0.008	3.402ValIle: 3.402 ± 0.013	3.94ValLys: 3.94 ± 0.013	6.247ValLeu: 6.247 ± 0.018	1.487ValMet: 1.487 ± 0.007	2.684ValAsn: 2.684 ± 0.01	3.257ValPro: 3.257 ± 0.011	2.318ValGln: 2.318 ± 0.011	2.985ValArg: 2.985 ± 0.011	5.533ValSer: 5.533 ± 0.014	3.21ValThr: 3.21 ± 0.011	4.719ValVal: 4.719 ± 0.017	0.731ValTrp: 0.731 ± 0.005	1.899ValTyr: 1.899 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	0.724TrpAla: 0.724 ± 0.005	0.232TrpCys: 0.232 ± 0.003	0.704TrpAsp: 0.704 ± 0.005	0.754TrpGlu: 0.754 ± 0.006	0.548TrpPhe: 0.548 ± 0.005	0.722TrpGly: 0.722 ± 0.006	0.294TrpHis: 0.294 ± 0.004	0.687TrpIle: 0.687 ± 0.006	0.921TrpLys: 0.921 ± 0.006	1.219TrpLeu: 1.219 ± 0.008	0.339TrpMet: 0.339 ± 0.004	0.675TrpAsn: 0.675 ± 0.006	0.494TrpPro: 0.494 ± 0.004	0.446TrpGln: 0.446 ± 0.005	0.801TrpArg: 0.801 ± 0.005	0.945TrpSer: 0.945 ± 0.006	0.596TrpThr: 0.596 ± 0.005	0.781TrpVal: 0.781 ± 0.006	0.244TrpTrp: 0.244 ± 0.003	0.346TrpTyr: 0.346 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.712TyrAla: 1.712 ± 0.007	0.638TyrCys: 0.638 ± 0.005	1.504TyrAsp: 1.504 ± 0.009	1.598TyrGlu: 1.598 ± 0.007	1.322TyrPhe: 1.322 ± 0.007	2.131TyrGly: 2.131 ± 0.01	0.73TyrHis: 0.73 ± 0.006	1.484TyrIle: 1.484 ± 0.009	1.553TyrLys: 1.553 ± 0.009	2.773TyrLeu: 2.773 ± 0.009	0.749TyrMet: 0.749 ± 0.005	1.309TyrAsn: 1.309 ± 0.008	1.272TyrPro: 1.272 ± 0.008	0.974TyrGln: 0.974 ± 0.006	1.47TyrArg: 1.47 ± 0.008	2.277TyrSer: 2.277 ± 0.01	1.236TyrThr: 1.236 ± 0.007	1.721TyrVal: 1.721 ± 0.008	0.399TyrTrp: 0.399 ± 0.004	0.972TyrTyr: 0.972 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.012XaaXaa: 0.012 ± 0.002

Statistics based on 66534 proteins (27687073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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