Anaerovirgula multivorans
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5186 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239A1S9|A0A239A1S9_9FIRM Flagellar biosynthetic protein FlhB OS=Anaerovirgula multivorans OX=312168 GN=flhB PE=3 SV=1
MM1 pKa = 7.05 NHH3 pKa = 6.79 LFEE6 pKa = 5.08 KK7 pKa = 10.36 VAYY10 pKa = 10.16 LKK12 pKa = 11.11 GLADD16 pKa = 4.21 GLDD19 pKa = 3.53 IEE21 pKa = 4.56 QSSKK25 pKa = 10.74 EE26 pKa = 4.19 GKK28 pKa = 10.08 LLLHH32 pKa = 7.24 IIDD35 pKa = 4.78 ALDD38 pKa = 4.09 DD39 pKa = 3.67 FAEE42 pKa = 5.39 AIVIMNEE49 pKa = 3.9 DD50 pKa = 3.59 QEE52 pKa = 5.41 DD53 pKa = 3.56 LTEE56 pKa = 4.07 YY57 pKa = 11.15 VEE59 pKa = 5.93 ALDD62 pKa = 4.65 EE63 pKa = 4.68 DD64 pKa = 4.3 LSDD67 pKa = 4.52 VEE69 pKa = 5.93 DD70 pKa = 4.42 EE71 pKa = 4.87 LFEE74 pKa = 7.04 DD75 pKa = 4.77 DD76 pKa = 6.62 DD77 pKa = 4.84 YY78 pKa = 12.19 DD79 pKa = 4.29 EE80 pKa = 6.88 DD81 pKa = 4.6 EE82 pKa = 6.41 DD83 pKa = 4.88 EE84 pKa = 6.21 DD85 pKa = 5.48 DD86 pKa = 6.4 DD87 pKa = 4.99 IDD89 pKa = 4.81 FMEE92 pKa = 5.03 IEE94 pKa = 4.88 CPNCDD99 pKa = 2.95 EE100 pKa = 5.06 EE101 pKa = 5.39 IFVDD105 pKa = 3.93 EE106 pKa = 4.95 DD107 pKa = 3.61 LFYY110 pKa = 11.43 DD111 pKa = 3.99 EE112 pKa = 5.3 GAEE115 pKa = 4.4 VTCPRR120 pKa = 11.84 CNKK123 pKa = 10.04 SIKK126 pKa = 10.04 LDD128 pKa = 3.64 EE129 pKa = 5.02 EE130 pKa = 4.73 FDD132 pKa = 4.02 CSCGHH137 pKa = 6.66 DD138 pKa = 3.77 HH139 pKa = 6.54 SHH141 pKa = 7.15 HH142 pKa = 6.03 EE143 pKa = 4.07
Molecular weight: 16.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.592
IPC_protein 3.605
Toseland 3.389
ProMoST 3.745
Dawson 3.592
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.427
Grimsley 3.3
Solomon 3.579
Lehninger 3.541
Nozaki 3.706
DTASelect 3.923
Thurlkill 3.439
EMBOSS 3.528
Sillero 3.719
Patrickios 0.846
IPC_peptide 3.579
IPC2_peptide 3.694
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A239EB94|A0A239EB94_9FIRM NADPH-dependent 7-cyano-7-deazaguanine reductase OS=Anaerovirgula multivorans OX=312168 GN=queF PE=3 SV=1
MM1 pKa = 7.8 AKK3 pKa = 9.94 QSLKK7 pKa = 10.66 VKK9 pKa = 8.54 QEE11 pKa = 3.64 RR12 pKa = 11.84 KK13 pKa = 8.56 QKK15 pKa = 10.18 YY16 pKa = 6.16 KK17 pKa = 8.61 TRR19 pKa = 11.84 EE20 pKa = 3.6 YY21 pKa = 9.89 TRR23 pKa = 11.84 CKK25 pKa = 9.69 ICGRR29 pKa = 11.84 PHH31 pKa = 6.72 AYY33 pKa = 9.43 LRR35 pKa = 11.84 KK36 pKa = 9.69 FGICRR41 pKa = 11.84 ICFRR45 pKa = 11.84 EE46 pKa = 3.97 LAYY49 pKa = 9.84 KK50 pKa = 10.14 GQIPGVRR57 pKa = 11.84 KK58 pKa = 10.13 ASWW61 pKa = 2.78
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.794
IPC_protein 10.145
Toseland 10.628
ProMoST 10.204
Dawson 10.745
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 11.228
Grimsley 10.789
Solomon 10.789
Lehninger 10.774
Nozaki 10.628
DTASelect 10.379
Thurlkill 10.628
EMBOSS 11.008
Sillero 10.657
Patrickios 10.965
IPC_peptide 10.789
IPC2_peptide 9.414
IPC2.peptide.svr19 8.497
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5186
0
5186
1509487
27
2837
291.1
32.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.391 ± 0.037
1.003 ± 0.013
5.176 ± 0.028
7.768 ± 0.038
4.208 ± 0.026
6.777 ± 0.037
1.745 ± 0.016
9.444 ± 0.035
7.834 ± 0.036
9.37 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.884 ± 0.018
5.135 ± 0.027
3.154 ± 0.021
3.077 ± 0.021
3.779 ± 0.025
5.716 ± 0.028
5.223 ± 0.03
6.654 ± 0.029
0.784 ± 0.012
3.878 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here