Amino acid dipepetide frequency for Novosphingobium sp. CF614

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.829AlaAla: 18.829 ± 0.182
1.199AlaCys: 1.199 ± 0.038
7.24AlaAsp: 7.24 ± 0.072
7.605AlaGlu: 7.605 ± 0.091
4.211AlaPhe: 4.211 ± 0.061
12.076AlaGly: 12.076 ± 0.118
2.394AlaHis: 2.394 ± 0.045
6.62AlaIle: 6.62 ± 0.079
3.69AlaLys: 3.69 ± 0.064
14.05AlaLeu: 14.05 ± 0.146
4.106AlaMet: 4.106 ± 0.063
3.034AlaAsn: 3.034 ± 0.066
6.261AlaPro: 6.261 ± 0.078
4.239AlaGln: 4.239 ± 0.066
10.599AlaArg: 10.599 ± 0.131
6.685AlaSer: 6.685 ± 0.082
5.963AlaThr: 5.963 ± 0.077
8.757AlaVal: 8.757 ± 0.083
1.836AlaTrp: 1.836 ± 0.038
2.568AlaTyr: 2.568 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.143CysAla: 1.143 ± 0.035
0.111CysCys: 0.111 ± 0.01
0.592CysAsp: 0.592 ± 0.022
0.488CysGlu: 0.488 ± 0.022
0.313CysPhe: 0.313 ± 0.015
0.992CysGly: 0.992 ± 0.029
0.284CysHis: 0.284 ± 0.03
0.383CysIle: 0.383 ± 0.019
0.196CysLys: 0.196 ± 0.014
0.793CysLeu: 0.793 ± 0.027
0.176CysMet: 0.176 ± 0.011
0.234CysAsn: 0.234 ± 0.014
0.503CysPro: 0.503 ± 0.023
0.248CysGln: 0.248 ± 0.015
0.644CysArg: 0.644 ± 0.024
0.478CysSer: 0.478 ± 0.022
0.42CysThr: 0.42 ± 0.02
0.579CysVal: 0.579 ± 0.025
0.129CysTrp: 0.129 ± 0.009
0.198CysTyr: 0.198 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
7.244AspAla: 7.244 ± 0.087
0.578AspCys: 0.578 ± 0.025
3.118AspAsp: 3.118 ± 0.069
3.371AspGlu: 3.371 ± 0.055
2.16AspPhe: 2.16 ± 0.045
5.446AspGly: 5.446 ± 0.094
1.281AspHis: 1.281 ± 0.033
2.904AspIle: 2.904 ± 0.049
1.724AspLys: 1.724 ± 0.036
5.788AspLeu: 5.788 ± 0.064
1.403AspMet: 1.403 ± 0.032
1.393AspAsn: 1.393 ± 0.036
3.928AspPro: 3.928 ± 0.063
1.669AspGln: 1.669 ± 0.042
4.634AspArg: 4.634 ± 0.063
2.316AspSer: 2.316 ± 0.043
2.582AspThr: 2.582 ± 0.057
3.911AspVal: 3.911 ± 0.066
1.156AspTrp: 1.156 ± 0.029
1.579AspTyr: 1.579 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
8.094GluAla: 8.094 ± 0.096
0.413GluCys: 0.413 ± 0.018
2.849GluAsp: 2.849 ± 0.053
3.114GluGlu: 3.114 ± 0.063
1.641GluPhe: 1.641 ± 0.038
4.557GluGly: 4.557 ± 0.064
1.25GluHis: 1.25 ± 0.029
3.073GluIle: 3.073 ± 0.048
1.991GluLys: 1.991 ± 0.053
5.164GluLeu: 5.164 ± 0.073
1.49GluMet: 1.49 ± 0.04
1.431GluAsn: 1.431 ± 0.03
2.737GluPro: 2.737 ± 0.047
2.14GluGln: 2.14 ± 0.045
4.876GluArg: 4.876 ± 0.074
2.408GluSer: 2.408 ± 0.045
3.18GluThr: 3.18 ± 0.051
3.761GluVal: 3.761 ± 0.057
0.791GluTrp: 0.791 ± 0.026
1.024GluTyr: 1.024 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.798PheAla: 4.798 ± 0.062
0.339PheCys: 0.339 ± 0.015
2.744PheAsp: 2.744 ± 0.051
2.006PheGlu: 2.006 ± 0.04
1.204PhePhe: 1.204 ± 0.031
3.719PheGly: 3.719 ± 0.059
0.771PheHis: 0.771 ± 0.024
1.395PheIle: 1.395 ± 0.037
0.863PheLys: 0.863 ± 0.03
2.992PheLeu: 2.992 ± 0.051
0.759PheMet: 0.759 ± 0.025
1.01PheAsn: 1.01 ± 0.031
1.559PhePro: 1.559 ± 0.038
0.901PheGln: 0.901 ± 0.025
2.219PheArg: 2.219 ± 0.044
2.017PheSer: 2.017 ± 0.042
2.014PheThr: 2.014 ± 0.045
2.618PheVal: 2.618 ± 0.049
0.514PheTrp: 0.514 ± 0.021
0.869PheTyr: 0.869 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
10.065GlyAla: 10.065 ± 0.098
0.865GlyCys: 0.865 ± 0.029
4.887GlyAsp: 4.887 ± 0.079
5.127GlyGlu: 5.127 ± 0.071
3.669GlyPhe: 3.669 ± 0.058
8.196GlyGly: 8.196 ± 0.117
1.897GlyHis: 1.897 ± 0.037
4.509GlyIle: 4.509 ± 0.066
3.469GlyLys: 3.469 ± 0.053
8.842GlyLeu: 8.842 ± 0.095
2.549GlyMet: 2.549 ± 0.042
2.574GlyAsn: 2.574 ± 0.078
3.964GlyPro: 3.964 ± 0.058
3.068GlyGln: 3.068 ± 0.049
6.6GlyArg: 6.6 ± 0.082
4.909GlySer: 4.909 ± 0.082
5.129GlyThr: 5.129 ± 0.103
6.084GlyVal: 6.084 ± 0.072
1.592GlyTrp: 1.592 ± 0.039
2.402GlyTyr: 2.402 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.6HisAla: 2.6 ± 0.054
0.271HisCys: 0.271 ± 0.013
1.267HisAsp: 1.267 ± 0.032
1.071HisGlu: 1.071 ± 0.03
0.829HisPhe: 0.829 ± 0.03
2.07HisGly: 2.07 ± 0.048
0.546HisHis: 0.546 ± 0.02
0.835HisIle: 0.835 ± 0.03
0.461HisLys: 0.461 ± 0.022
1.98HisLeu: 1.98 ± 0.04
0.477HisMet: 0.477 ± 0.02
0.475HisAsn: 0.475 ± 0.021
1.331HisPro: 1.331 ± 0.034
0.538HisGln: 0.538 ± 0.02
1.524HisArg: 1.524 ± 0.036
0.935HisSer: 0.935 ± 0.026
0.746HisThr: 0.746 ± 0.022
1.595HisVal: 1.595 ± 0.039
0.31HisTrp: 0.31 ± 0.015
0.598HisTyr: 0.598 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.652IleAla: 7.652 ± 0.095
0.453IleCys: 0.453 ± 0.017
3.911IleAsp: 3.911 ± 0.059
3.64IleGlu: 3.64 ± 0.052
1.427IlePhe: 1.427 ± 0.035
4.948IleGly: 4.948 ± 0.07
0.892IleHis: 0.892 ± 0.027
1.695IleIle: 1.695 ± 0.042
1.181IleLys: 1.181 ± 0.031
3.783IleLeu: 3.783 ± 0.059
0.875IleMet: 0.875 ± 0.029
1.291IleAsn: 1.291 ± 0.04
2.278IlePro: 2.278 ± 0.044
1.106IleGln: 1.106 ± 0.034
3.123IleArg: 3.123 ± 0.05
2.615IleSer: 2.615 ± 0.052
2.401IleThr: 2.401 ± 0.049
3.936IleVal: 3.936 ± 0.061
0.579IleTrp: 0.579 ± 0.025
1.011IleTyr: 1.011 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
4.019LysAla: 4.019 ± 0.065
0.163LysCys: 0.163 ± 0.01
1.538LysAsp: 1.538 ± 0.037
1.288LysGlu: 1.288 ± 0.036
0.811LysPhe: 0.811 ± 0.027
2.617LysGly: 2.617 ± 0.045
0.553LysHis: 0.553 ± 0.019
1.366LysIle: 1.366 ± 0.038
0.935LysLys: 0.935 ± 0.034
3.097LysLeu: 3.097 ± 0.053
0.673LysMet: 0.673 ± 0.024
0.704LysAsn: 0.704 ± 0.026
1.944LysPro: 1.944 ± 0.041
0.926LysGln: 0.926 ± 0.029
2.076LysArg: 2.076 ± 0.047
1.548LysSer: 1.548 ± 0.038
1.512LysThr: 1.512 ± 0.042
2.312LysVal: 2.312 ± 0.053
0.402LysTrp: 0.402 ± 0.018
0.608LysTyr: 0.608 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
14.876LeuAla: 14.876 ± 0.15
0.863LeuCys: 0.863 ± 0.026
6.266LeuAsp: 6.266 ± 0.075
5.155LeuGlu: 5.155 ± 0.075
3.472LeuPhe: 3.472 ± 0.055
8.528LeuGly: 8.528 ± 0.092
1.905LeuHis: 1.905 ± 0.049
4.266LeuIle: 4.266 ± 0.066
2.883LeuLys: 2.883 ± 0.05
9.346LeuLeu: 9.346 ± 0.13
2.112LeuMet: 2.112 ± 0.047
2.239LeuAsn: 2.239 ± 0.052
5.74LeuPro: 5.74 ± 0.086
2.443LeuGln: 2.443 ± 0.052
7.121LeuArg: 7.121 ± 0.089
5.973LeuSer: 5.973 ± 0.072
5.387LeuThr: 5.387 ± 0.083
7.472LeuVal: 7.472 ± 0.096
1.151LeuTrp: 1.151 ± 0.027
1.952LeuTyr: 1.952 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
3.393MetAla: 3.393 ± 0.057
0.15MetCys: 0.15 ± 0.012
1.143MetAsp: 1.143 ± 0.028
1.131MetGlu: 1.131 ± 0.032
0.691MetPhe: 0.691 ± 0.024
1.948MetGly: 1.948 ± 0.047
0.454MetHis: 0.454 ± 0.018
1.354MetIle: 1.354 ± 0.036
0.955MetLys: 0.955 ± 0.024
2.708MetLeu: 2.708 ± 0.057
0.667MetMet: 0.667 ± 0.027
0.741MetAsn: 0.741 ± 0.021
1.561MetPro: 1.561 ± 0.039
0.743MetGln: 0.743 ± 0.025
1.915MetArg: 1.915 ± 0.038
1.43MetSer: 1.43 ± 0.041
1.754MetThr: 1.754 ± 0.036
1.696MetVal: 1.696 ± 0.036
0.218MetTrp: 0.218 ± 0.013
0.25MetTyr: 0.25 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.232AsnAla: 3.232 ± 0.064
0.309AsnCys: 0.309 ± 0.015
1.339AsnAsp: 1.339 ± 0.042
1.118AsnGlu: 1.118 ± 0.03
0.958AsnPhe: 0.958 ± 0.029
2.423AsnGly: 2.423 ± 0.06
0.49AsnHis: 0.49 ± 0.02
1.272AsnIle: 1.272 ± 0.041
0.587AsnLys: 0.587 ± 0.023
2.516AsnLeu: 2.516 ± 0.056
0.547AsnMet: 0.547 ± 0.023
0.632AsnAsn: 0.632 ± 0.028
1.818AsnPro: 1.818 ± 0.039
0.737AsnGln: 0.737 ± 0.029
1.853AsnArg: 1.853 ± 0.041
1.224AsnSer: 1.224 ± 0.034
1.175AsnThr: 1.175 ± 0.039
1.881AsnVal: 1.881 ± 0.053
0.406AsnTrp: 0.406 ± 0.019
0.731AsnTyr: 0.731 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
7.285ProAla: 7.285 ± 0.09
0.408ProCys: 0.408 ± 0.019
3.645ProAsp: 3.645 ± 0.06
3.827ProGlu: 3.827 ± 0.067
1.98ProPhe: 1.98 ± 0.047
5.3ProGly: 5.3 ± 0.082
1.035ProHis: 1.035 ± 0.03
2.39ProIle: 2.39 ± 0.047
1.396ProLys: 1.396 ± 0.042
4.986ProLeu: 4.986 ± 0.071
1.224ProMet: 1.224 ± 0.034
1.202ProAsn: 1.202 ± 0.032
2.98ProPro: 2.98 ± 0.07
1.706ProGln: 1.706 ± 0.034
3.482ProArg: 3.482 ± 0.044
2.852ProSer: 2.852 ± 0.053
2.34ProThr: 2.34 ± 0.042
4.275ProVal: 4.275 ± 0.058
0.739ProTrp: 0.739 ± 0.025
1.075ProTyr: 1.075 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
4.284GlnAla: 4.284 ± 0.064
0.25GlnCys: 0.25 ± 0.013
1.475GlnAsp: 1.475 ± 0.035
1.344GlnGlu: 1.344 ± 0.034
1.058GlnPhe: 1.058 ± 0.024
2.674GlnGly: 2.674 ± 0.05
0.596GlnHis: 0.596 ± 0.025
1.659GlnIle: 1.659 ± 0.036
0.803GlnLys: 0.803 ± 0.023
2.891GlnLeu: 2.891 ± 0.048
0.791GlnMet: 0.791 ± 0.028
0.705GlnAsn: 0.705 ± 0.025
1.744GlnPro: 1.744 ± 0.039
1.106GlnGln: 1.106 ± 0.034
2.312GlnArg: 2.312 ± 0.044
1.648GlnSer: 1.648 ± 0.039
1.547GlnThr: 1.547 ± 0.035
2.491GlnVal: 2.491 ± 0.043
0.454GlnTrp: 0.454 ± 0.017
0.624GlnTyr: 0.624 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
8.814ArgAla: 8.814 ± 0.099
0.576ArgCys: 0.576 ± 0.021
4.166ArgAsp: 4.166 ± 0.057
4.442ArgGlu: 4.442 ± 0.076
3.184ArgPhe: 3.184 ± 0.053
5.38ArgGly: 5.38 ± 0.059
1.834ArgHis: 1.834 ± 0.041
4.314ArgIle: 4.314 ± 0.055
2.466ArgLys: 2.466 ± 0.048
8.404ArgLeu: 8.404 ± 0.093
2.053ArgMet: 2.053 ± 0.042
1.822ArgAsn: 1.822 ± 0.036
3.725ArgPro: 3.725 ± 0.062
2.569ArgGln: 2.569 ± 0.05
5.993ArgArg: 5.993 ± 0.088
3.516ArgSer: 3.516 ± 0.056
3.437ArgThr: 3.437 ± 0.059
4.83ArgVal: 4.83 ± 0.076
1.235ArgTrp: 1.235 ± 0.032
1.903ArgTyr: 1.903 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
6.488SerAla: 6.488 ± 0.08
0.481SerCys: 0.481 ± 0.023
2.913SerAsp: 2.913 ± 0.057
2.658SerGlu: 2.658 ± 0.047
2.108SerPhe: 2.108 ± 0.044
5.579SerGly: 5.579 ± 0.074
1.033SerHis: 1.033 ± 0.029
2.535SerIle: 2.535 ± 0.055
1.331SerLys: 1.331 ± 0.036
5.323SerLeu: 5.323 ± 0.066
1.233SerMet: 1.233 ± 0.034
1.421SerAsn: 1.421 ± 0.039
2.928SerPro: 2.928 ± 0.049
1.603SerGln: 1.603 ± 0.035
3.757SerArg: 3.757 ± 0.065
2.94SerSer: 2.94 ± 0.07
2.767SerThr: 2.767 ± 0.058
3.545SerVal: 3.545 ± 0.058
0.772SerTrp: 0.772 ± 0.026
1.37SerTyr: 1.37 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
6.124ThrAla: 6.124 ± 0.098
0.45ThrCys: 0.45 ± 0.021
2.739ThrAsp: 2.739 ± 0.057
2.356ThrGlu: 2.356 ± 0.047
1.816ThrPhe: 1.816 ± 0.04
5.424ThrGly: 5.424 ± 0.094
0.911ThrHis: 0.911 ± 0.032
2.836ThrIle: 2.836 ± 0.054
1.139ThrLys: 1.139 ± 0.036
5.371ThrLeu: 5.371 ± 0.075
1.255ThrMet: 1.255 ± 0.032
1.223ThrAsn: 1.223 ± 0.042
3.261ThrPro: 3.261 ± 0.059
1.434ThrGln: 1.434 ± 0.035
3.548ThrArg: 3.548 ± 0.056
2.84ThrSer: 2.84 ± 0.07
2.66ThrThr: 2.66 ± 0.061
4.122ThrVal: 4.122 ± 0.062
0.682ThrTrp: 0.682 ± 0.024
1.237ThrTyr: 1.237 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
8.93ValAla: 8.93 ± 0.095
0.642ValCys: 0.642 ± 0.025
4.077ValAsp: 4.077 ± 0.058
4.475ValGlu: 4.475 ± 0.062
2.301ValPhe: 2.301 ± 0.044
5.48ValGly: 5.48 ± 0.079
1.449ValHis: 1.449 ± 0.032
3.795ValIle: 3.795 ± 0.048
1.94ValLys: 1.94 ± 0.046
7.212ValLeu: 7.212 ± 0.092
1.764ValMet: 1.764 ± 0.036
1.998ValAsn: 1.998 ± 0.047
4.05ValPro: 4.05 ± 0.064
1.931ValGln: 1.931 ± 0.039
5.13ValArg: 5.13 ± 0.069
4.282ValSer: 4.282 ± 0.059
4.536ValThr: 4.536 ± 0.086
5.239ValVal: 5.239 ± 0.084
0.851ValTrp: 0.851 ± 0.026
1.335ValTyr: 1.335 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.417TrpAla: 1.417 ± 0.035
0.141TrpCys: 0.141 ± 0.011
0.707TrpAsp: 0.707 ± 0.022
0.601TrpGlu: 0.601 ± 0.021
0.568TrpPhe: 0.568 ± 0.024
0.939TrpGly: 0.939 ± 0.029
0.38TrpHis: 0.38 ± 0.019
0.757TrpIle: 0.757 ± 0.025
0.532TrpLys: 0.532 ± 0.021
1.776TrpLeu: 1.776 ± 0.039
0.327TrpMet: 0.327 ± 0.017
0.494TrpAsn: 0.494 ± 0.019
0.727TrpPro: 0.727 ± 0.028
0.611TrpGln: 0.611 ± 0.022
1.375TrpArg: 1.375 ± 0.037
0.909TrpSer: 0.909 ± 0.025
0.825TrpThr: 0.825 ± 0.025
0.785TrpVal: 0.785 ± 0.024
0.246TrpTrp: 0.246 ± 0.013
0.301TrpTyr: 0.301 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.677TyrAla: 2.677 ± 0.055
0.246TyrCys: 0.246 ± 0.015
1.569TyrAsp: 1.569 ± 0.056
1.169TyrGlu: 1.169 ± 0.032
0.911TyrPhe: 0.911 ± 0.027
2.101TyrGly: 2.101 ± 0.048
0.505TyrHis: 0.505 ± 0.021
0.839TyrIle: 0.839 ± 0.028
0.552TyrLys: 0.552 ± 0.023
2.127TyrLeu: 2.127 ± 0.044
0.419TyrMet: 0.419 ± 0.017
0.622TyrAsn: 0.622 ± 0.029
1.089TyrPro: 1.089 ± 0.032
0.738TyrGln: 0.738 ± 0.026
1.878TyrArg: 1.878 ± 0.041
1.255TyrSer: 1.255 ± 0.036
1.056TyrThr: 1.056 ± 0.032
1.524TyrVal: 1.524 ± 0.041
0.356TyrTrp: 0.356 ± 0.018
0.668TyrTyr: 0.668 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3840 proteins (1249785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski