Hubei picorna-like virus 25
Average proteome isoelectric point is 5.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KKV6|A0A1L3KKV6_9VIRU RNA-directed RNA polymerase OS=Hubei picorna-like virus 25 OX=1923105 PE=4 SV=1
MM1 pKa = 7.06 VHH3 pKa = 7.04 ASWILKK9 pKa = 8.15 RR10 pKa = 11.84 TTPAEE15 pKa = 4.16 TNEE18 pKa = 4.32 SQNSNGGVISSGDD31 pKa = 3.5 DD32 pKa = 3.04 SVQNVIYY39 pKa = 9.81 TEE41 pKa = 4.06 PTMTTSQTYY50 pKa = 10.86 GDD52 pKa = 4.05 NQFSTLVDD60 pKa = 4.05 QIDD63 pKa = 3.53 EE64 pKa = 4.47 TYY66 pKa = 10.38 SIKK69 pKa = 10.6 KK70 pKa = 8.63 ILEE73 pKa = 3.99 MPSRR77 pKa = 11.84 ISTWQFKK84 pKa = 9.75 TDD86 pKa = 3.32 SLTLFDD92 pKa = 4.53 SPLPRR97 pKa = 11.84 FTLDD101 pKa = 3.37 FPKK104 pKa = 10.83 DD105 pKa = 3.26 ALTTSKK111 pKa = 10.4 KK112 pKa = 9.3 QKK114 pKa = 9.96 FYY116 pKa = 11.34 NFAYY120 pKa = 9.71 TKK122 pKa = 10.58 FDD124 pKa = 3.41 VKK126 pKa = 11.1 LKK128 pKa = 10.49 FLVSAPKK135 pKa = 7.66 TTSGIFWICYY145 pKa = 9.33 SPYY148 pKa = 10.38 EE149 pKa = 4.11 KK150 pKa = 10.38 QLEE153 pKa = 4.17 SRR155 pKa = 11.84 YY156 pKa = 10.28 KK157 pKa = 10.26 IEE159 pKa = 4.08 NRR161 pKa = 11.84 DD162 pKa = 3.35 IAGVTQYY169 pKa = 11.17 PGVKK173 pKa = 10.11 LNLAEE178 pKa = 4.26 STSAEE183 pKa = 4.0 LVIPYY188 pKa = 9.86 VDD190 pKa = 3.21 YY191 pKa = 11.35 RR192 pKa = 11.84 EE193 pKa = 4.6 AYY195 pKa = 10.24 NILSDD200 pKa = 3.82 SDD202 pKa = 4.01 SSVTLNVYY210 pKa = 10.5 LLAPIRR216 pKa = 11.84 NVTTSSQNITMTVEE230 pKa = 3.82 ASLTNISLTCPVNLLPAEE248 pKa = 3.95 NSIWKK253 pKa = 8.49 MQGLGEE259 pKa = 4.18 GGRR262 pKa = 11.84 RR263 pKa = 11.84 GVISEE268 pKa = 4.11 ATGMISGIANTVSNIPVVGAIAKK291 pKa = 9.16 PVGFVSDD298 pKa = 3.79 LVGGVASLLGFSKK311 pKa = 10.82 PITFEE316 pKa = 3.66 QQQNVSNLPGRR327 pKa = 11.84 GYY329 pKa = 11.07 AHH331 pKa = 6.98 SKK333 pKa = 10.57 GVDD336 pKa = 3.24 NSVMLGMSQEE346 pKa = 4.23 TEE348 pKa = 3.85 LALVTDD354 pKa = 4.66 EE355 pKa = 5.1 FPSKK359 pKa = 10.2 EE360 pKa = 4.08 DD361 pKa = 3.51 EE362 pKa = 4.11 MDD364 pKa = 3.13 IDD366 pKa = 4.34 TILRR370 pKa = 11.84 TPGLANTSEE379 pKa = 4.2 WVDD382 pKa = 3.37 GEE384 pKa = 5.11 TYY386 pKa = 10.02 TIWHH390 pKa = 6.1 VPFLFKK396 pKa = 10.6 WEE398 pKa = 4.15 DD399 pKa = 3.27 TGNDD403 pKa = 3.25 QVQKK407 pKa = 9.71 LTNTSYY413 pKa = 11.18 FEE415 pKa = 3.97 YY416 pKa = 10.26 LAMMFKK422 pKa = 10.57 YY423 pKa = 10.03 FRR425 pKa = 11.84 ADD427 pKa = 2.62 IVYY430 pKa = 8.69 TFEE433 pKa = 3.65 ISKK436 pKa = 9.04 TAFHH440 pKa = 6.68 AGKK443 pKa = 10.52 LEE445 pKa = 4.12 LYY447 pKa = 9.63 YY448 pKa = 11.09 CSGTNGYY455 pKa = 10.63 SSTDD459 pKa = 2.93 PSTTYY464 pKa = 10.25 RR465 pKa = 11.84 VVWDD469 pKa = 3.4 ISEE472 pKa = 4.59 SNTLTFKK479 pKa = 10.8 IPFTYY484 pKa = 10.52 NKK486 pKa = 10.16 AFMPVAVDD494 pKa = 3.23 NSEE497 pKa = 4.03 YY498 pKa = 11.0 GSLIIRR504 pKa = 11.84 NVTPLMYY511 pKa = 10.7 NEE513 pKa = 4.13 GVEE516 pKa = 4.24 GKK518 pKa = 10.42 VDD520 pKa = 3.42 ILCWKK525 pKa = 10.06 HH526 pKa = 5.06 YY527 pKa = 10.26 EE528 pKa = 4.43 NVNFMCPSPNYY539 pKa = 9.6 VQFSSTSPTPNEE551 pKa = 4.12 PNDD554 pKa = 3.66 GQTYY558 pKa = 9.48 KK559 pKa = 11.07 AAGTLIYY566 pKa = 10.76 DD567 pKa = 4.26 LFRR570 pKa = 11.84 GNITGYY576 pKa = 10.29 RR577 pKa = 11.84 YY578 pKa = 10.53 NEE580 pKa = 4.18 DD581 pKa = 2.99 QTLNIWFDD589 pKa = 3.92 SEE591 pKa = 4.25 GGNHH595 pKa = 6.87 HH596 pKa = 7.08 IIKK599 pKa = 10.21 FDD601 pKa = 3.74 EE602 pKa = 4.31 EE603 pKa = 4.16 TTALINRR610 pKa = 11.84 PTEE613 pKa = 4.06 FGKK616 pKa = 8.56 TRR618 pKa = 11.84 YY619 pKa = 9.48 YY620 pKa = 11.14 DD621 pKa = 3.26 STVQYY626 pKa = 10.19 GATMDD631 pKa = 3.55 DD632 pKa = 3.95 TIIVIINLIEE642 pKa = 3.97 LVIYY646 pKa = 10.05 DD647 pKa = 4.35 ALFVPPAANWRR658 pKa = 11.84 SQILIEE664 pKa = 4.29 EE665 pKa = 4.43 EE666 pKa = 4.12 GNKK669 pKa = 10.33 YY670 pKa = 10.49 SVSTLEE676 pKa = 3.95 TSQNSISVLTQVGGEE691 pKa = 3.86 IVKK694 pKa = 9.95 NLRR697 pKa = 11.84 YY698 pKa = 8.38 ITRR701 pKa = 11.84 SHH703 pKa = 7.27 RR704 pKa = 11.84 PTQLFDD710 pKa = 3.72 LAANQQISLNFYY722 pKa = 10.57 SNDD725 pKa = 3.31 NIIEE729 pKa = 4.12 VDD731 pKa = 3.21 TLTYY735 pKa = 9.89 FSYY738 pKa = 9.7 MYY740 pKa = 11.19 RR741 pKa = 11.84 MMRR744 pKa = 11.84 GGIRR748 pKa = 11.84 YY749 pKa = 8.92 KK750 pKa = 10.76 VFNTSRR756 pKa = 11.84 QGYY759 pKa = 8.59 SFSALSDD766 pKa = 3.79 SIKK769 pKa = 10.6 PGLISNLTYY778 pKa = 11.01 NDD780 pKa = 4.29 LNPVHH785 pKa = 6.33 EE786 pKa = 4.6 VEE788 pKa = 4.73 VPYY791 pKa = 10.96 YY792 pKa = 8.51 NQNRR796 pKa = 11.84 RR797 pKa = 11.84 FIVGLEE803 pKa = 3.93 QPRR806 pKa = 11.84 DD807 pKa = 3.76 VPQGAVYY814 pKa = 8.36 ATSEE818 pKa = 4.33 TSPGTLITQAGSDD831 pKa = 3.87 DD832 pKa = 3.77 FTFGLRR838 pKa = 11.84 VGPPKK843 pKa = 10.32 IFRR846 pKa = 11.84 LKK848 pKa = 10.27 NAPEE852 pKa = 3.98 VKK854 pKa = 9.22 TPKK857 pKa = 9.74 VRR859 pKa = 11.84 PFTTLNPAVGSNACRR874 pKa = 11.84 RR875 pKa = 11.84 SVPLPRR881 pKa = 11.84 IEE883 pKa = 4.52 EE884 pKa = 4.13 EE885 pKa = 4.13 RR886 pKa = 11.84 TPTAA890 pKa = 3.8
Molecular weight: 99.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.915
IPC2_protein 5.029
IPC_protein 4.978
Toseland 4.863
ProMoST 5.092
Dawson 4.94
Bjellqvist 5.08
Wikipedia 4.8
Rodwell 4.851
Grimsley 4.787
Solomon 4.94
Lehninger 4.889
Nozaki 5.054
DTASelect 5.194
Thurlkill 4.863
EMBOSS 4.825
Sillero 5.118
Patrickios 3.681
IPC_peptide 4.94
IPC2_peptide 5.118
IPC2.peptide.svr19 5.066
Protein with the highest isoelectric point:
>tr|A0A1L3KKV6|A0A1L3KKV6_9VIRU RNA-directed RNA polymerase OS=Hubei picorna-like virus 25 OX=1923105 PE=4 SV=1
MM1 pKa = 7.14 THH3 pKa = 6.58 PTQNLNQLNKK13 pKa = 8.32 QQRR16 pKa = 11.84 TEE18 pKa = 4.07 LKK20 pKa = 9.69 TLLKK24 pKa = 10.18 QIKK27 pKa = 9.13 KK28 pKa = 10.11 LKK30 pKa = 8.41 PDD32 pKa = 3.43 EE33 pKa = 4.04 MLEE36 pKa = 4.72 LIDD39 pKa = 5.16 YY40 pKa = 10.46 LDD42 pKa = 3.51 KK43 pKa = 11.0 TADD46 pKa = 3.63 LLVIFDD52 pKa = 3.88 EE53 pKa = 4.66 THH55 pKa = 6.84 CKK57 pKa = 8.6 VTTNGTTRR65 pKa = 11.84 IYY67 pKa = 11.03 SRR69 pKa = 11.84 TGNDD73 pKa = 3.06 LRR75 pKa = 11.84 DD76 pKa = 3.64 VIMTLFFGIEE86 pKa = 3.7 QHH88 pKa = 6.96 DD89 pKa = 4.67 DD90 pKa = 2.57 NWNAQGIFDD99 pKa = 5.05 FSITHH104 pKa = 5.58 NVKK107 pKa = 7.73 TTPEE111 pKa = 3.87 IEE113 pKa = 4.05 RR114 pKa = 11.84 VIVDD118 pKa = 3.29 SADD121 pKa = 3.86 RR122 pKa = 11.84 LNTMAQQITDD132 pKa = 2.91 IFEE135 pKa = 4.05 TFKK138 pKa = 11.45 AKK140 pKa = 10.3 IGDD143 pKa = 4.44 NIPSALSSLIKK154 pKa = 10.35 HH155 pKa = 6.02 ISEE158 pKa = 4.34 AVLVSIMNRR167 pKa = 11.84 KK168 pKa = 9.73 KK169 pKa = 10.26 FMKK172 pKa = 10.37 VFTTTIAAKK181 pKa = 10.12 ILIYY185 pKa = 10.62 CIEE188 pKa = 4.18 TGIKK192 pKa = 10.48 YY193 pKa = 10.54 LDD195 pKa = 4.13 LFNMAKK201 pKa = 10.36 NLITKK206 pKa = 8.99 GQEE209 pKa = 3.79 CWSSQGLTDD218 pKa = 4.96 FDD220 pKa = 4.69 FSSIQIEE227 pKa = 4.29 KK228 pKa = 10.1 ILNIKK233 pKa = 8.14 TLAIPFLFTFLISSFYY249 pKa = 10.85 KK250 pKa = 10.1 IFTSGTPTTEE260 pKa = 4.1 LVTATWKK267 pKa = 9.39 TFTKK271 pKa = 10.41 LPKK274 pKa = 9.26 EE275 pKa = 4.03 HH276 pKa = 7.05 SNILNTLKK284 pKa = 10.82 DD285 pKa = 3.28 ILNFIGEE292 pKa = 4.54 FFNINVGKK300 pKa = 9.88 NAIKK304 pKa = 10.22 IKK306 pKa = 10.19 CSRR309 pKa = 11.84 LLEE312 pKa = 5.57 DD313 pKa = 3.0 ITEE316 pKa = 4.29 MIKK319 pKa = 10.39 IKK321 pKa = 10.92 DD322 pKa = 3.71 NVGGQSRR329 pKa = 11.84 DD330 pKa = 3.19 QDD332 pKa = 3.7 EE333 pKa = 3.92 ISYY336 pKa = 10.23 VFEE339 pKa = 4.12 RR340 pKa = 11.84 AEE342 pKa = 4.14 KK343 pKa = 10.74 AFSLYY348 pKa = 10.61 EE349 pKa = 4.03 NYY351 pKa = 10.73 LEE353 pKa = 5.61 LVGEE357 pKa = 4.31 CEE359 pKa = 3.76 KK360 pKa = 10.55 WGLKK364 pKa = 10.62 AEE366 pKa = 4.41 VKK368 pKa = 10.22 RR369 pKa = 11.84 LQAIKK374 pKa = 8.74 TQVEE378 pKa = 4.17 QLMKK382 pKa = 10.46 HH383 pKa = 5.32 YY384 pKa = 10.02 LAHH387 pKa = 7.01 AKK389 pKa = 9.94 YY390 pKa = 9.35 LQKK393 pKa = 10.64 DD394 pKa = 3.51 RR395 pKa = 11.84 KK396 pKa = 9.9 VPIFVLMRR404 pKa = 11.84 GEE406 pKa = 4.24 PGCGKK411 pKa = 8.03 TQFLNLLSNFASKK424 pKa = 10.16 LHH426 pKa = 6.16 KK427 pKa = 10.36 PEE429 pKa = 6.33 LEE431 pKa = 3.96 DD432 pKa = 3.76 KK433 pKa = 11.24 YY434 pKa = 10.86 RR435 pKa = 11.84 ITDD438 pKa = 3.33 IFTPALGSEE447 pKa = 4.54 YY448 pKa = 10.79 NDD450 pKa = 3.63 GYY452 pKa = 11.09 YY453 pKa = 10.03 PGVTSYY459 pKa = 11.02 IFDD462 pKa = 4.38 DD463 pKa = 4.55 LGTVADD469 pKa = 4.51 SVANPDD475 pKa = 3.31 PTFANLINMVNNAPFSLNMSKK496 pKa = 10.85 AEE498 pKa = 4.03 EE499 pKa = 3.93 KK500 pKa = 11.03 GKK502 pKa = 10.47 IFFDD506 pKa = 3.7 SPFIFATTNQEE517 pKa = 3.35 KK518 pKa = 10.37 WNTKK522 pKa = 9.96 SINNAGALARR532 pKa = 11.84 RR533 pKa = 11.84 TYY535 pKa = 11.35 LDD537 pKa = 3.51 ILVEE541 pKa = 4.19 PNPDD545 pKa = 3.36 LVHH548 pKa = 6.67 TYY550 pKa = 10.23 IKK552 pKa = 10.7 DD553 pKa = 3.22 GQTCVKK559 pKa = 10.21 LDD561 pKa = 3.59 EE562 pKa = 4.84 AKK564 pKa = 10.15 IDD566 pKa = 3.75 ALNVAEE572 pKa = 4.26 GHH574 pKa = 6.68 PPGHH578 pKa = 6.26 YY579 pKa = 9.91 NPKK582 pKa = 10.28 CSTLKK587 pKa = 10.86 VLLCGQLTRR596 pKa = 11.84 QEE598 pKa = 4.08 MSVNDD603 pKa = 3.81 ILEE606 pKa = 4.33 LLAEE610 pKa = 4.42 EE611 pKa = 4.9 YY612 pKa = 10.66 YY613 pKa = 10.13 RR614 pKa = 11.84 RR615 pKa = 11.84 KK616 pKa = 9.57 WSVNMKK622 pKa = 10.15 EE623 pKa = 3.96 STLNIVKK630 pKa = 10.46 DD631 pKa = 4.36 FEE633 pKa = 5.61 DD634 pKa = 3.46 KK635 pKa = 11.07 LATIEE640 pKa = 4.39 AKK642 pKa = 10.65 YY643 pKa = 9.95 KK644 pKa = 10.26 SQMLEE649 pKa = 3.98 EE650 pKa = 4.46 EE651 pKa = 4.75 EE652 pKa = 4.5 EE653 pKa = 4.0 FHH655 pKa = 7.82 DD656 pKa = 4.78 ALEE659 pKa = 4.9 KK660 pKa = 9.09 QTFWNTFTTVTPEE673 pKa = 3.33 RR674 pKa = 11.84 HH675 pKa = 5.48 LVNNRR680 pKa = 11.84 YY681 pKa = 9.08 LAMMQKK687 pKa = 9.99 INPDD691 pKa = 2.85 SSYY694 pKa = 10.87 NQAVRR699 pKa = 11.84 MKK701 pKa = 10.55 NLFEE705 pKa = 4.22 VVEE708 pKa = 4.31 VVYY711 pKa = 9.23 NTTKK715 pKa = 10.26 TVANSIMTTFSNLWEE730 pKa = 4.13 KK731 pKa = 8.83 FTWKK735 pKa = 8.79 KK736 pKa = 8.94 TFLASFSLITLGLGYY751 pKa = 10.3 LAYY754 pKa = 10.6 NYY756 pKa = 6.94 WTKK759 pKa = 10.32 EE760 pKa = 3.61 APRR763 pKa = 11.84 RR764 pKa = 11.84 EE765 pKa = 4.39 SQFRR769 pKa = 11.84 LYY771 pKa = 11.0 VVDD774 pKa = 5.87 NEE776 pKa = 4.33 WDD778 pKa = 3.15 WTILDD783 pKa = 3.75 KK784 pKa = 11.2 EE785 pKa = 4.52 KK786 pKa = 11.23 YY787 pKa = 10.25 LDD789 pKa = 3.62 FAKK792 pKa = 10.66 EE793 pKa = 3.3 ITLAKK798 pKa = 10.31 NIEE801 pKa = 4.21 KK802 pKa = 10.06 HH803 pKa = 4.93 WGKK806 pKa = 10.76 CYY808 pKa = 10.6 LINSLGAEE816 pKa = 4.11 LFDD819 pKa = 4.34 PFVIDD824 pKa = 5.29 VITNYY829 pKa = 10.68 AKK831 pKa = 10.73 YY832 pKa = 8.44 RR833 pKa = 11.84 THH835 pKa = 6.67 FALGVRR841 pKa = 11.84 RR842 pKa = 11.84 RR843 pKa = 11.84 DD844 pKa = 3.34 MEE846 pKa = 4.18 KK847 pKa = 9.85 LRR849 pKa = 11.84 KK850 pKa = 9.06 IISVMRR856 pKa = 11.84 IAKK859 pKa = 10.06 KK860 pKa = 10.27 FDD862 pKa = 3.42 GFDD865 pKa = 3.35 EE866 pKa = 4.74 LSIGDD871 pKa = 3.48 AHH873 pKa = 7.55 DD874 pKa = 3.82 IFSEE878 pKa = 4.08 SRR880 pKa = 11.84 VYY882 pKa = 11.16 NDD884 pKa = 4.68 DD885 pKa = 3.37 VNKK888 pKa = 9.42 PRR890 pKa = 11.84 RR891 pKa = 11.84 NIEE894 pKa = 4.14 SKK896 pKa = 9.75 TYY898 pKa = 10.66 NPDD901 pKa = 3.18 GNKK904 pKa = 9.32 VRR906 pKa = 11.84 VNIEE910 pKa = 4.03 SKK912 pKa = 9.76 TYY914 pKa = 10.79 NPDD917 pKa = 3.06 GNKK920 pKa = 9.73 PKK922 pKa = 10.83 VNIEE926 pKa = 3.83 SRR928 pKa = 11.84 VYY930 pKa = 11.11 NDD932 pKa = 3.51 DD933 pKa = 3.55 TNVPKK938 pKa = 10.74 RR939 pKa = 11.84 NIEE942 pKa = 4.05 GLDD945 pKa = 3.21 KK946 pKa = 11.65 SNFLDD951 pKa = 3.86 GLKK954 pKa = 9.41 TLFSQGHH961 pKa = 6.25 IDD963 pKa = 3.44 STANALLRR971 pKa = 11.84 NRR973 pKa = 11.84 IPQSTGVVAVYY984 pKa = 10.38 DD985 pKa = 3.86 SNEE988 pKa = 3.79 RR989 pKa = 11.84 KK990 pKa = 9.5 IGSMHH995 pKa = 6.35 LTMLKK1000 pKa = 10.44 GRR1002 pKa = 11.84 IGVTASHH1009 pKa = 7.15 LLLGKK1014 pKa = 9.79 PSFLKK1019 pKa = 10.7 VYY1021 pKa = 9.44 FVNKK1025 pKa = 9.66 QPYY1028 pKa = 8.49 EE1029 pKa = 3.97 YY1030 pKa = 10.29 SISEE1034 pKa = 4.3 LVFHH1038 pKa = 7.44 EE1039 pKa = 4.35 DD1040 pKa = 2.89 TSRR1043 pKa = 11.84 DD1044 pKa = 3.36 LLFIEE1049 pKa = 5.15 FPNRR1053 pKa = 11.84 HH1054 pKa = 5.45 EE1055 pKa = 4.09 QFKK1058 pKa = 10.87 DD1059 pKa = 2.78 ITKK1062 pKa = 10.22 QFVIKK1067 pKa = 10.57 SDD1069 pKa = 3.9 FKK1071 pKa = 11.62 HH1072 pKa = 4.77 MTNTKK1077 pKa = 9.51 GALYY1081 pKa = 8.61 TYY1083 pKa = 10.13 PNLADD1088 pKa = 4.39 CDD1090 pKa = 4.15 LSRR1093 pKa = 11.84 SCYY1096 pKa = 10.2 KK1097 pKa = 10.46 SGNVYY1102 pKa = 10.66 AVTTDD1107 pKa = 3.85 YY1108 pKa = 11.32 VIHH1111 pKa = 6.76 TSLKK1115 pKa = 10.62 DD1116 pKa = 3.21 EE1117 pKa = 4.06 TAGIVRR1123 pKa = 11.84 RR1124 pKa = 11.84 LDD1126 pKa = 3.21 GLTYY1130 pKa = 10.34 DD1131 pKa = 3.5 INTDD1135 pKa = 3.65 FGEE1138 pKa = 4.6 CGSCLVMANPMLPRR1152 pKa = 11.84 KK1153 pKa = 9.31 IAGIHH1158 pKa = 6.29 IAGQDD1163 pKa = 3.52 ASPSGLASVITQEE1176 pKa = 3.92 YY1177 pKa = 9.17 LEE1179 pKa = 4.39 EE1180 pKa = 4.28 VFVRR1184 pKa = 11.84 CNWKK1188 pKa = 9.82 SQVSYY1193 pKa = 10.66 QIEE1196 pKa = 4.4 KK1197 pKa = 10.18 IYY1199 pKa = 11.14 NLDD1202 pKa = 3.77 SEE1204 pKa = 5.46 SEE1206 pKa = 4.04 LSNIIPEE1213 pKa = 4.43 APCHH1217 pKa = 5.34 GVSILGKK1224 pKa = 10.08 IIDD1227 pKa = 4.44 SPRR1230 pKa = 11.84 QPTKK1234 pKa = 10.45 TSITRR1239 pKa = 11.84 SAIHH1243 pKa = 6.38 GVFPPEE1249 pKa = 3.98 TKK1251 pKa = 10.03 PVNLRR1256 pKa = 11.84 NTPEE1260 pKa = 3.55 FDD1262 pKa = 3.12 IFRR1265 pKa = 11.84 LNSKK1269 pKa = 10.2 KK1270 pKa = 10.21 ISLPTKK1276 pKa = 10.47 KK1277 pKa = 9.29 IDD1279 pKa = 3.52 EE1280 pKa = 4.78 KK1281 pKa = 9.29 YY1282 pKa = 10.91 LRR1284 pKa = 11.84 MAVRR1288 pKa = 11.84 DD1289 pKa = 3.41 VCRR1292 pKa = 11.84 TVNIGQDD1299 pKa = 3.14 ATLVRR1304 pKa = 11.84 VFDD1307 pKa = 4.05 TEE1309 pKa = 4.03 TAIAGIPNEE1318 pKa = 4.28 KK1319 pKa = 10.41 YY1320 pKa = 9.16 VDD1322 pKa = 3.58 GVKK1325 pKa = 10.29 RR1326 pKa = 11.84 QTSPGYY1332 pKa = 9.63 PYY1334 pKa = 10.44 IKK1336 pKa = 10.46 AAGGDD1341 pKa = 4.2 GKK1343 pKa = 9.69 RR1344 pKa = 11.84 TWLGYY1349 pKa = 10.76 DD1350 pKa = 3.15 GDD1352 pKa = 4.38 YY1353 pKa = 10.83 IIDD1356 pKa = 3.88 KK1357 pKa = 10.82 DD1358 pKa = 4.18 VVDD1361 pKa = 5.01 LVDD1364 pKa = 4.31 IVIDD1368 pKa = 3.5 NAKK1371 pKa = 10.26 NLEE1374 pKa = 4.0 RR1375 pKa = 11.84 TPNVFVDD1382 pKa = 3.54 SLKK1385 pKa = 10.9 DD1386 pKa = 3.39 EE1387 pKa = 4.45 RR1388 pKa = 11.84 NVIEE1392 pKa = 5.34 KK1393 pKa = 10.34 VDD1395 pKa = 3.51 AGKK1398 pKa = 8.24 TRR1400 pKa = 11.84 IFSNGDD1406 pKa = 3.71 FIHH1409 pKa = 6.75 TIAMRR1414 pKa = 11.84 KK1415 pKa = 6.45 YY1416 pKa = 7.43 TLGFSAHH1423 pKa = 6.24 LMRR1426 pKa = 11.84 GKK1428 pKa = 9.81 IEE1430 pKa = 4.04 NEE1432 pKa = 3.9 SAVGIDD1438 pKa = 4.52 VHH1440 pKa = 6.1 TEE1442 pKa = 3.47 WKK1444 pKa = 10.24 KK1445 pKa = 10.71 LADD1448 pKa = 3.94 FLLRR1452 pKa = 11.84 DD1453 pKa = 3.67 NKK1455 pKa = 10.31 TKK1457 pKa = 10.46 FVAGDD1462 pKa = 3.23 FSGFDD1467 pKa = 3.49 GTLNAQILWAILDD1480 pKa = 4.08 VIEE1483 pKa = 4.77 DD1484 pKa = 4.14 FYY1486 pKa = 12.05 YY1487 pKa = 10.97 NATEE1491 pKa = 3.47 EE1492 pKa = 4.21 DD1493 pKa = 3.14 RR1494 pKa = 11.84 RR1495 pKa = 11.84 VRR1497 pKa = 11.84 YY1498 pKa = 9.88 VLFSDD1503 pKa = 5.27 LINSIRR1509 pKa = 11.84 LQNGVLYY1516 pKa = 10.5 QCDD1519 pKa = 3.44 HH1520 pKa = 6.35 SQPSGNPLTVILNTLYY1536 pKa = 11.47 NMIAIRR1542 pKa = 11.84 IVYY1545 pKa = 7.46 LICKK1549 pKa = 9.06 KK1550 pKa = 9.67 KK1551 pKa = 10.86 ASMRR1555 pKa = 11.84 PHH1557 pKa = 6.85 LSDD1560 pKa = 3.0 FRR1562 pKa = 11.84 KK1563 pKa = 9.7 YY1564 pKa = 10.79 VNFVAYY1570 pKa = 10.13 GDD1572 pKa = 4.19 DD1573 pKa = 3.55 NLYY1576 pKa = 11.27 GIDD1579 pKa = 4.32 DD1580 pKa = 3.97 CMIDD1584 pKa = 3.35 IFNQHH1589 pKa = 5.36 TVTAAFAEE1597 pKa = 4.21 IGMVYY1602 pKa = 9.88 TDD1604 pKa = 3.55 EE1605 pKa = 5.64 AKK1607 pKa = 10.27 TNDD1610 pKa = 3.46 GSVPPYY1616 pKa = 9.4 RR1617 pKa = 11.84 TFSEE1621 pKa = 4.26 VGFLKK1626 pKa = 9.99 RR1627 pKa = 11.84 TFGKK1631 pKa = 10.75 LEE1633 pKa = 3.54 IGFRR1637 pKa = 11.84 YY1638 pKa = 9.08 PNIYY1642 pKa = 10.29 LMCPIAQRR1650 pKa = 11.84 TIRR1653 pKa = 11.84 EE1654 pKa = 4.09 LCNWTKK1660 pKa = 10.79 RR1661 pKa = 11.84 SDD1663 pKa = 4.13 DD1664 pKa = 3.83 NLQATIDD1671 pKa = 3.87 NIEE1674 pKa = 4.26 DD1675 pKa = 3.56 AFHH1678 pKa = 6.08 EE1679 pKa = 4.37 AFYY1682 pKa = 10.72 HH1683 pKa = 7.47 GEE1685 pKa = 3.76 DD1686 pKa = 3.88 FYY1688 pKa = 11.84 NGFTEE1693 pKa = 4.8 HH1694 pKa = 5.88 VSKK1697 pKa = 9.72 TFKK1700 pKa = 10.51 HH1701 pKa = 5.74 YY1702 pKa = 9.91 CTINKK1707 pKa = 8.78 LRR1709 pKa = 11.84 ANLKK1713 pKa = 8.9 VKK1715 pKa = 10.11 SWDD1718 pKa = 3.67 EE1719 pKa = 3.95 LCDD1722 pKa = 3.44 EE1723 pKa = 4.16 HH1724 pKa = 9.07 VEE1726 pKa = 4.45 RR1727 pKa = 11.84 NFPQTGVLMM1736 pKa = 4.91
Molecular weight: 198.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.127
IPC2_protein 6.148
IPC_protein 6.211
Toseland 6.491
ProMoST 6.605
Dawson 6.532
Bjellqvist 6.547
Wikipedia 6.547
Rodwell 6.532
Grimsley 6.634
Solomon 6.532
Lehninger 6.532
Nozaki 6.825
DTASelect 6.985
Thurlkill 7.0
EMBOSS 6.971
Sillero 6.927
Patrickios 4.266
IPC_peptide 6.547
IPC2_peptide 6.707
IPC2.peptide.svr19 6.78
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
2626
890
1736
1313.0
149.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.179 ± 0.175
1.142 ± 0.236
6.245 ± 0.6
6.131 ± 0.089
5.027 ± 0.212
5.331 ± 0.371
1.79 ± 0.393
7.426 ± 0.345
6.816 ± 1.171
8.264 ± 0.428
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.018 ± 0.002
6.436 ± 0.016
4.075 ± 0.665
3.046 ± 0.277
4.379 ± 0.169
6.664 ± 1.059
7.959 ± 0.689
6.359 ± 0.306
1.142 ± 0.047
4.57 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here