Amino acid dipepetide frequency for Cyanophage KBS-S-2A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.898AlaAla: 12.898 ± 1.691
0.472AlaCys: 0.472 ± 0.202
5.977AlaAsp: 5.977 ± 0.851
5.741AlaGlu: 5.741 ± 0.807
3.696AlaPhe: 3.696 ± 0.588
7.629AlaGly: 7.629 ± 0.917
1.022AlaHis: 1.022 ± 0.269
6.292AlaIle: 6.292 ± 0.712
4.797AlaLys: 4.797 ± 0.923
8.966AlaLeu: 8.966 ± 1.21
1.809AlaMet: 1.809 ± 0.42
3.932AlaAsn: 3.932 ± 0.514
3.618AlaPro: 3.618 ± 0.572
4.404AlaGln: 4.404 ± 0.672
4.797AlaArg: 4.797 ± 0.772
6.134AlaSer: 6.134 ± 0.781
7.471AlaThr: 7.471 ± 0.808
6.449AlaVal: 6.449 ± 0.584
1.494AlaTrp: 1.494 ± 0.288
3.225AlaTyr: 3.225 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
0.944CysAla: 0.944 ± 0.29
0.157CysCys: 0.157 ± 0.088
0.157CysAsp: 0.157 ± 0.091
0.315CysGlu: 0.315 ± 0.169
0.236CysPhe: 0.236 ± 0.176
0.708CysGly: 0.708 ± 0.269
0.236CysHis: 0.236 ± 0.131
0.865CysIle: 0.865 ± 0.258
0.551CysLys: 0.551 ± 0.2
0.629CysLeu: 0.629 ± 0.204
0.315CysMet: 0.315 ± 0.133
0.708CysAsn: 0.708 ± 0.208
0.393CysPro: 0.393 ± 0.163
0.393CysGln: 0.393 ± 0.227
1.101CysArg: 1.101 ± 0.335
0.472CysSer: 0.472 ± 0.207
0.315CysThr: 0.315 ± 0.17
0.393CysVal: 0.393 ± 0.199
0.315CysTrp: 0.315 ± 0.129
0.315CysTyr: 0.315 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
5.82AspAla: 5.82 ± 0.783
0.551AspCys: 0.551 ± 0.224
4.404AspAsp: 4.404 ± 0.617
3.067AspGlu: 3.067 ± 0.475
2.359AspPhe: 2.359 ± 0.426
6.134AspGly: 6.134 ± 0.863
0.708AspHis: 0.708 ± 0.28
3.46AspIle: 3.46 ± 0.416
1.573AspLys: 1.573 ± 0.326
5.348AspLeu: 5.348 ± 0.741
0.865AspMet: 0.865 ± 0.303
2.91AspAsn: 2.91 ± 0.565
2.674AspPro: 2.674 ± 0.434
2.281AspGln: 2.281 ± 0.418
3.46AspArg: 3.46 ± 0.554
3.539AspSer: 3.539 ± 0.54
3.539AspThr: 3.539 ± 0.473
4.011AspVal: 4.011 ± 0.577
0.786AspTrp: 0.786 ± 0.266
1.888AspTyr: 1.888 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
5.899GluAla: 5.899 ± 0.661
0.551GluCys: 0.551 ± 0.221
2.595GluAsp: 2.595 ± 0.503
2.753GluGlu: 2.753 ± 0.45
1.494GluPhe: 1.494 ± 0.266
3.618GluGly: 3.618 ± 0.528
1.18GluHis: 1.18 ± 0.331
4.168GluIle: 4.168 ± 0.633
2.674GluLys: 2.674 ± 0.515
6.213GluLeu: 6.213 ± 0.772
0.708GluMet: 0.708 ± 0.231
2.281GluAsn: 2.281 ± 0.398
2.202GluPro: 2.202 ± 0.502
4.011GluGln: 4.011 ± 0.714
4.011GluArg: 4.011 ± 0.569
3.067GluSer: 3.067 ± 0.36
3.382GluThr: 3.382 ± 0.522
3.382GluVal: 3.382 ± 0.655
0.944GluTrp: 0.944 ± 0.251
1.337GluTyr: 1.337 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
2.989PheAla: 2.989 ± 0.423
0.236PheCys: 0.236 ± 0.139
2.674PheAsp: 2.674 ± 0.397
2.045PheGlu: 2.045 ± 0.354
0.551PhePhe: 0.551 ± 0.206
2.674PheGly: 2.674 ± 0.44
0.629PheHis: 0.629 ± 0.321
1.416PheIle: 1.416 ± 0.385
1.652PheLys: 1.652 ± 0.339
1.573PheLeu: 1.573 ± 0.289
0.944PheMet: 0.944 ± 0.31
1.337PheAsn: 1.337 ± 0.311
1.416PhePro: 1.416 ± 0.372
1.337PheGln: 1.337 ± 0.274
2.438PheArg: 2.438 ± 0.402
2.438PheSer: 2.438 ± 0.369
2.91PheThr: 2.91 ± 0.459
1.966PheVal: 1.966 ± 0.495
0.786PheTrp: 0.786 ± 0.225
0.944PheTyr: 0.944 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
6.921GlyAla: 6.921 ± 0.821
0.629GlyCys: 0.629 ± 0.207
4.404GlyAsp: 4.404 ± 0.653
4.247GlyGlu: 4.247 ± 0.681
3.067GlyPhe: 3.067 ± 0.583
7.078GlyGly: 7.078 ± 1.275
0.944GlyHis: 0.944 ± 0.387
4.247GlyIle: 4.247 ± 0.474
3.775GlyLys: 3.775 ± 0.584
5.82GlyLeu: 5.82 ± 0.826
1.73GlyMet: 1.73 ± 0.353
2.989GlyAsn: 2.989 ± 0.566
2.045GlyPro: 2.045 ± 0.407
2.753GlyGln: 2.753 ± 0.513
3.067GlyArg: 3.067 ± 0.528
5.584GlySer: 5.584 ± 0.872
7.865GlyThr: 7.865 ± 1.365
4.326GlyVal: 4.326 ± 0.516
0.786GlyTrp: 0.786 ± 0.24
3.067GlyTyr: 3.067 ± 0.596
0.0GlyXaa: 0.0 ± 0.0
His
1.337HisAla: 1.337 ± 0.357
0.472HisCys: 0.472 ± 0.242
0.629HisAsp: 0.629 ± 0.228
0.708HisGlu: 0.708 ± 0.276
0.551HisPhe: 0.551 ± 0.225
0.944HisGly: 0.944 ± 0.353
0.315HisHis: 0.315 ± 0.168
0.551HisIle: 0.551 ± 0.204
0.315HisLys: 0.315 ± 0.188
1.101HisLeu: 1.101 ± 0.285
0.315HisMet: 0.315 ± 0.168
0.472HisAsn: 0.472 ± 0.185
1.101HisPro: 1.101 ± 0.289
0.865HisGln: 0.865 ± 0.231
1.258HisArg: 1.258 ± 0.387
0.944HisSer: 0.944 ± 0.385
0.786HisThr: 0.786 ± 0.267
1.101HisVal: 1.101 ± 0.226
0.157HisTrp: 0.157 ± 0.112
0.944HisTyr: 0.944 ± 0.312
0.0HisXaa: 0.0 ± 0.0
Ile
6.134IleAla: 6.134 ± 0.771
0.551IleCys: 0.551 ± 0.254
4.09IleAsp: 4.09 ± 0.564
4.326IleGlu: 4.326 ± 0.583
1.652IlePhe: 1.652 ± 0.354
3.854IleGly: 3.854 ± 0.688
0.472IleHis: 0.472 ± 0.224
2.831IleIle: 2.831 ± 0.465
2.753IleLys: 2.753 ± 0.542
3.618IleLeu: 3.618 ± 0.611
0.393IleMet: 0.393 ± 0.156
2.831IleAsn: 2.831 ± 0.467
2.989IlePro: 2.989 ± 0.469
2.595IleGln: 2.595 ± 0.419
3.146IleArg: 3.146 ± 0.561
4.483IleSer: 4.483 ± 0.474
5.505IleThr: 5.505 ± 0.956
4.011IleVal: 4.011 ± 0.571
0.708IleTrp: 0.708 ± 0.194
1.652IleTyr: 1.652 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
5.033LysAla: 5.033 ± 1.001
0.472LysCys: 0.472 ± 0.162
2.674LysAsp: 2.674 ± 0.473
1.966LysGlu: 1.966 ± 0.343
1.494LysPhe: 1.494 ± 0.281
4.09LysGly: 4.09 ± 0.804
0.551LysHis: 0.551 ± 0.173
2.517LysIle: 2.517 ± 0.422
2.202LysLys: 2.202 ± 0.444
4.404LysLeu: 4.404 ± 0.573
0.786LysMet: 0.786 ± 0.205
1.966LysAsn: 1.966 ± 0.454
2.202LysPro: 2.202 ± 0.432
1.966LysGln: 1.966 ± 0.584
2.91LysArg: 2.91 ± 0.552
2.753LysSer: 2.753 ± 0.428
3.067LysThr: 3.067 ± 0.452
2.595LysVal: 2.595 ± 0.539
0.472LysTrp: 0.472 ± 0.174
1.18LysTyr: 1.18 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
8.179LeuAla: 8.179 ± 0.966
1.022LeuCys: 1.022 ± 0.345
4.797LeuAsp: 4.797 ± 0.646
5.899LeuGlu: 5.899 ± 0.855
2.123LeuPhe: 2.123 ± 0.362
4.955LeuGly: 4.955 ± 0.924
1.966LeuHis: 1.966 ± 0.42
4.326LeuIle: 4.326 ± 0.592
3.618LeuLys: 3.618 ± 0.479
5.977LeuLeu: 5.977 ± 0.676
1.022LeuMet: 1.022 ± 0.313
4.326LeuAsn: 4.326 ± 0.468
4.168LeuPro: 4.168 ± 0.643
4.719LeuGln: 4.719 ± 0.532
5.348LeuArg: 5.348 ± 0.582
6.134LeuSer: 6.134 ± 0.66
6.213LeuThr: 6.213 ± 0.75
4.404LeuVal: 4.404 ± 0.692
0.708LeuTrp: 0.708 ± 0.21
1.652LeuTyr: 1.652 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
2.359MetAla: 2.359 ± 0.548
0.0MetCys: 0.0 ± 0.0
0.786MetAsp: 0.786 ± 0.279
0.944MetGlu: 0.944 ± 0.284
0.0MetPhe: 0.0 ± 0.0
0.472MetGly: 0.472 ± 0.231
0.551MetHis: 0.551 ± 0.229
0.708MetIle: 0.708 ± 0.247
1.494MetLys: 1.494 ± 0.375
1.258MetLeu: 1.258 ± 0.357
0.236MetMet: 0.236 ± 0.115
0.551MetAsn: 0.551 ± 0.221
1.022MetPro: 1.022 ± 0.283
0.865MetGln: 0.865 ± 0.255
0.786MetArg: 0.786 ± 0.286
1.416MetSer: 1.416 ± 0.349
1.652MetThr: 1.652 ± 0.437
1.101MetVal: 1.101 ± 0.237
0.0MetTrp: 0.0 ± 0.0
0.786MetTyr: 0.786 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.404AsnAla: 4.404 ± 0.605
0.472AsnCys: 0.472 ± 0.146
2.674AsnAsp: 2.674 ± 0.486
1.73AsnGlu: 1.73 ± 0.388
1.258AsnPhe: 1.258 ± 0.281
4.326AsnGly: 4.326 ± 0.598
0.629AsnHis: 0.629 ± 0.236
1.809AsnIle: 1.809 ± 0.356
1.652AsnLys: 1.652 ± 0.424
3.854AsnLeu: 3.854 ± 0.491
0.786AsnMet: 0.786 ± 0.252
1.966AsnAsn: 1.966 ± 0.369
3.382AsnPro: 3.382 ± 0.506
2.281AsnGln: 2.281 ± 0.414
2.281AsnArg: 2.281 ± 0.422
2.674AsnSer: 2.674 ± 0.449
3.146AsnThr: 3.146 ± 0.423
2.359AsnVal: 2.359 ± 0.433
1.101AsnTrp: 1.101 ± 0.274
1.337AsnTyr: 1.337 ± 0.329
0.0AsnXaa: 0.0 ± 0.0
Pro
4.562ProAla: 4.562 ± 0.577
0.551ProCys: 0.551 ± 0.171
3.854ProAsp: 3.854 ± 0.635
2.595ProGlu: 2.595 ± 0.464
1.888ProPhe: 1.888 ± 0.375
2.674ProGly: 2.674 ± 0.458
0.472ProHis: 0.472 ± 0.224
1.888ProIle: 1.888 ± 0.377
1.652ProLys: 1.652 ± 0.43
2.989ProLeu: 2.989 ± 0.472
1.101ProMet: 1.101 ± 0.314
2.359ProAsn: 2.359 ± 0.506
2.281ProPro: 2.281 ± 0.533
1.888ProGln: 1.888 ± 0.455
1.888ProArg: 1.888 ± 0.393
2.438ProSer: 2.438 ± 0.581
4.168ProThr: 4.168 ± 0.569
2.517ProVal: 2.517 ± 0.391
0.786ProTrp: 0.786 ± 0.244
1.966ProTyr: 1.966 ± 0.357
0.0ProXaa: 0.0 ± 0.0
Gln
4.876GlnAla: 4.876 ± 0.706
0.079GlnCys: 0.079 ± 0.079
2.045GlnAsp: 2.045 ± 0.351
2.674GlnGlu: 2.674 ± 0.501
1.494GlnPhe: 1.494 ± 0.392
2.989GlnGly: 2.989 ± 0.437
0.472GlnHis: 0.472 ± 0.181
3.618GlnIle: 3.618 ± 0.438
1.888GlnLys: 1.888 ± 0.342
5.584GlnLeu: 5.584 ± 0.744
0.944GlnMet: 0.944 ± 0.244
2.438GlnAsn: 2.438 ± 0.511
1.966GlnPro: 1.966 ± 0.347
2.674GlnGln: 2.674 ± 0.44
2.989GlnArg: 2.989 ± 0.524
3.225GlnSer: 3.225 ± 0.435
2.359GlnThr: 2.359 ± 0.44
3.775GlnVal: 3.775 ± 0.767
1.258GlnTrp: 1.258 ± 0.382
1.18GlnTyr: 1.18 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
5.269ArgAla: 5.269 ± 0.69
0.472ArgCys: 0.472 ± 0.205
2.989ArgAsp: 2.989 ± 0.58
2.753ArgGlu: 2.753 ± 0.466
2.281ArgPhe: 2.281 ± 0.472
3.146ArgGly: 3.146 ± 0.487
1.022ArgHis: 1.022 ± 0.27
4.326ArgIle: 4.326 ± 0.627
3.225ArgLys: 3.225 ± 0.564
4.719ArgLeu: 4.719 ± 0.743
1.416ArgMet: 1.416 ± 0.294
1.888ArgAsn: 1.888 ± 0.316
2.123ArgPro: 2.123 ± 0.415
2.989ArgGln: 2.989 ± 0.555
4.09ArgArg: 4.09 ± 0.704
3.225ArgSer: 3.225 ± 0.393
3.46ArgThr: 3.46 ± 0.415
3.303ArgVal: 3.303 ± 0.501
0.393ArgTrp: 0.393 ± 0.185
2.595ArgTyr: 2.595 ± 0.586
0.0ArgXaa: 0.0 ± 0.0
Ser
5.899SerAla: 5.899 ± 0.64
0.865SerCys: 0.865 ± 0.245
3.932SerAsp: 3.932 ± 0.512
3.696SerGlu: 3.696 ± 0.726
3.225SerPhe: 3.225 ± 0.512
6.449SerGly: 6.449 ± 0.855
1.101SerHis: 1.101 ± 0.351
4.483SerIle: 4.483 ± 0.544
2.674SerLys: 2.674 ± 0.404
4.719SerLeu: 4.719 ± 0.634
1.101SerMet: 1.101 ± 0.325
2.359SerAsn: 2.359 ± 0.378
2.753SerPro: 2.753 ± 0.467
2.831SerGln: 2.831 ± 0.487
3.225SerArg: 3.225 ± 0.502
4.483SerSer: 4.483 ± 0.859
6.37SerThr: 6.37 ± 0.854
4.247SerVal: 4.247 ± 0.678
1.022SerTrp: 1.022 ± 0.328
2.202SerTyr: 2.202 ± 0.38
0.0SerXaa: 0.0 ± 0.0
Thr
8.101ThrAla: 8.101 ± 1.041
0.472ThrCys: 0.472 ± 0.195
4.09ThrAsp: 4.09 ± 0.525
4.326ThrGlu: 4.326 ± 0.502
2.517ThrPhe: 2.517 ± 0.405
6.449ThrGly: 6.449 ± 0.944
0.865ThrHis: 0.865 ± 0.274
5.191ThrIle: 5.191 ± 0.61
3.932ThrLys: 3.932 ± 0.59
5.348ThrLeu: 5.348 ± 0.626
0.865ThrMet: 0.865 ± 0.245
3.225ThrAsn: 3.225 ± 0.528
4.168ThrPro: 4.168 ± 0.694
4.797ThrGln: 4.797 ± 0.547
2.831ThrArg: 2.831 ± 0.427
5.741ThrSer: 5.741 ± 0.735
6.606ThrThr: 6.606 ± 1.002
5.033ThrVal: 5.033 ± 0.712
1.101ThrTrp: 1.101 ± 0.327
2.281ThrTyr: 2.281 ± 0.506
0.0ThrXaa: 0.0 ± 0.0
Val
5.663ValAla: 5.663 ± 0.852
0.786ValCys: 0.786 ± 0.264
3.225ValAsp: 3.225 ± 0.542
3.775ValGlu: 3.775 ± 0.534
2.045ValPhe: 2.045 ± 0.421
4.64ValGly: 4.64 ± 0.645
0.786ValHis: 0.786 ± 0.329
3.067ValIle: 3.067 ± 0.426
3.146ValLys: 3.146 ± 0.462
5.191ValLeu: 5.191 ± 0.539
0.629ValMet: 0.629 ± 0.207
2.753ValAsn: 2.753 ± 0.334
2.753ValPro: 2.753 ± 0.441
2.517ValGln: 2.517 ± 0.409
3.618ValArg: 3.618 ± 0.527
5.269ValSer: 5.269 ± 0.768
5.505ValThr: 5.505 ± 0.702
4.247ValVal: 4.247 ± 0.595
0.708ValTrp: 0.708 ± 0.247
2.517ValTyr: 2.517 ± 0.525
0.0ValXaa: 0.0 ± 0.0
Trp
1.18TrpAla: 1.18 ± 0.282
0.236TrpCys: 0.236 ± 0.125
1.022TrpAsp: 1.022 ± 0.311
0.315TrpGlu: 0.315 ± 0.149
0.157TrpPhe: 0.157 ± 0.109
0.472TrpGly: 0.472 ± 0.227
0.315TrpHis: 0.315 ± 0.166
0.944TrpIle: 0.944 ± 0.234
0.472TrpLys: 0.472 ± 0.163
1.337TrpLeu: 1.337 ± 0.376
0.551TrpMet: 0.551 ± 0.215
0.786TrpAsn: 0.786 ± 0.182
0.393TrpPro: 0.393 ± 0.166
0.708TrpGln: 0.708 ± 0.213
0.708TrpArg: 0.708 ± 0.213
1.416TrpSer: 1.416 ± 0.292
0.786TrpThr: 0.786 ± 0.274
1.258TrpVal: 1.258 ± 0.282
0.157TrpTrp: 0.157 ± 0.104
0.786TrpTyr: 0.786 ± 0.223
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.359TyrAla: 2.359 ± 0.475
0.472TyrCys: 0.472 ± 0.179
2.359TyrAsp: 2.359 ± 0.392
2.595TyrGlu: 2.595 ± 0.532
0.865TyrPhe: 0.865 ± 0.256
2.045TyrGly: 2.045 ± 0.471
0.629TyrHis: 0.629 ± 0.249
1.888TyrIle: 1.888 ± 0.362
1.258TyrLys: 1.258 ± 0.297
2.989TyrLeu: 2.989 ± 0.547
0.472TyrMet: 0.472 ± 0.169
2.045TyrAsn: 2.045 ± 0.407
0.865TyrPro: 0.865 ± 0.313
1.652TyrGln: 1.652 ± 0.407
1.652TyrArg: 1.652 ± 0.432
2.202TyrSer: 2.202 ± 0.569
2.831TyrThr: 2.831 ± 0.504
2.359TyrVal: 2.359 ± 0.51
0.315TyrTrp: 0.315 ± 0.152
1.101TyrTyr: 1.101 ± 0.306
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12716 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski