Streptococcus phage CHPC663

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F6U5|A0A3G8F6U5_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC663 OX=2365039 GN=CHPC663_0036 PE=4 SV=1
MM1 pKa = 7.12VNWVDD6 pKa = 4.58KK7 pKa = 11.1DD8 pKa = 4.24GNDD11 pKa = 3.97LPDD14 pKa = 4.77GADD17 pKa = 3.18QDD19 pKa = 4.63FKK21 pKa = 11.75AGMFFSFAGDD31 pKa = 3.56EE32 pKa = 4.15NNITDD37 pKa = 3.96TGNGGYY43 pKa = 10.37YY44 pKa = 9.73GGYY47 pKa = 9.69YY48 pKa = 9.72YY49 pKa = 10.64RR50 pKa = 11.84QFEE53 pKa = 4.2FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F6Z3|A0A3G8F6Z3_9CAUD Transcriptional Cro repressor OS=Streptococcus phage CHPC663 OX=2365039 GN=CHPC663_0031 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.48NYY4 pKa = 10.03FEE6 pKa = 4.3KK7 pKa = 11.05LGVKK11 pKa = 8.49VLKK14 pKa = 10.07TMAQSAVGVIGASTLISQVDD34 pKa = 3.15WRR36 pKa = 11.84AQRR39 pKa = 11.84KK40 pKa = 6.7YY41 pKa = 10.52RR42 pKa = 11.84RR43 pKa = 11.84SS44 pKa = 3.23

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11503

44

1551

221.2

25.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.468 ± 0.571

0.574 ± 0.157

6.633 ± 0.287

6.329 ± 0.456

4.155 ± 0.219

7.076 ± 0.564

1.46 ± 0.122

6.407 ± 0.236

8.476 ± 0.486

7.737 ± 0.412

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.205

6.155 ± 0.244

2.982 ± 0.216

4.286 ± 0.254

4.121 ± 0.287

6.79 ± 0.357

6.372 ± 0.327

5.677 ± 0.233

1.495 ± 0.153

4.251 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski