Auraticoccus cholistanensis
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3358 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A9UWB0|A0A6A9UWB0_9ACTN Fe-S cluster assembly ATPase SufC OS=Auraticoccus cholistanensis OX=2656650 GN=sufC PE=3 SV=1
MM1 pKa = 7.18 TALFMSMFSAGVAHH15 pKa = 7.47 AATVYY20 pKa = 10.13 EE21 pKa = 5.62 IEE23 pKa = 4.67 GEE25 pKa = 4.26 WEE27 pKa = 4.02 AGTPTTVGRR36 pKa = 11.84 GDD38 pKa = 3.64 VVTGIWRR45 pKa = 11.84 VNVNDD50 pKa = 4.83 DD51 pKa = 4.1 AEE53 pKa = 5.16 APANDD58 pKa = 3.62 PVEE61 pKa = 4.26 NVLFSVTLDD70 pKa = 3.01 NGVFRR75 pKa = 11.84 EE76 pKa = 4.25 LPDD79 pKa = 3.3 VCVTGDD85 pKa = 3.51 EE86 pKa = 4.4 VDD88 pKa = 3.82 PQSTLSEE95 pKa = 4.47 DD96 pKa = 4.02 NRR98 pKa = 11.84 TLTCNLGTVEE108 pKa = 4.04 QGSAVVIQTPVVVDD122 pKa = 3.96 GLTGEE127 pKa = 4.09 QLTAVGEE134 pKa = 4.46 INGQEE139 pKa = 4.19 QPLPPLNIVNEE150 pKa = 4.33 FGMDD154 pKa = 3.07 IQFGQNTGSYY164 pKa = 8.69 YY165 pKa = 10.28 WNDD168 pKa = 4.25 DD169 pKa = 3.51 YY170 pKa = 11.81 TQLDD174 pKa = 3.28 VDD176 pKa = 5.12 LNWSLRR182 pKa = 11.84 LAAGSDD188 pKa = 3.69 PGPDD192 pKa = 2.87 SVTYY196 pKa = 10.53 RR197 pKa = 11.84 LQLTEE202 pKa = 4.32 TNNGTIEE209 pKa = 4.78 PGTGLYY215 pKa = 10.74 GDD217 pKa = 4.42 VGCGPFNAYY226 pKa = 8.78 IANGHH231 pKa = 6.32 PWSEE235 pKa = 4.11 LPNYY239 pKa = 9.46 PADD242 pKa = 3.72 QQTSFVDD249 pKa = 3.52 SCTLTPVAGQPGLFDD264 pKa = 3.79 LTLDD268 pKa = 4.71 GINYY272 pKa = 10.06 DD273 pKa = 3.87 LATVPTKK280 pKa = 10.95 DD281 pKa = 3.21 SAGNPLPTDD290 pKa = 3.45 WSYY293 pKa = 11.47 IASGSLWFSITTNRR307 pKa = 11.84 AGSIALQTNAPTYY320 pKa = 10.51 EE321 pKa = 4.12 STTGLFSTDD330 pKa = 2.81 LTEE333 pKa = 5.32 NNTTTKK339 pKa = 10.62 SYY341 pKa = 9.11 TLPGRR346 pKa = 11.84 WSAPWYY352 pKa = 9.7 RR353 pKa = 11.84 GHH355 pKa = 6.77 TGAGGTNWDD364 pKa = 3.11 DD365 pKa = 3.79 TYY367 pKa = 11.22 RR368 pKa = 11.84 VSAGQTVAQFVNNTASRR385 pKa = 11.84 LGDD388 pKa = 3.46 VPDD391 pKa = 3.87 EE392 pKa = 4.38 QIHH395 pKa = 5.1 GQCLVFDD402 pKa = 4.47 TAYY405 pKa = 10.76 SSYY408 pKa = 11.1 ASPEE412 pKa = 4.13 GEE414 pKa = 4.08 RR415 pKa = 11.84 VPMIRR420 pKa = 11.84 GYY422 pKa = 9.96 DD423 pKa = 3.36 QEE425 pKa = 4.75 GAGGAAEE432 pKa = 4.74 LEE434 pKa = 4.19 NPPTLEE440 pKa = 4.23 YY441 pKa = 11.1 YY442 pKa = 10.69 VGTVTDD448 pKa = 4.67 PNAFVCGNDD457 pKa = 3.21 PGNWVDD463 pKa = 5.22 EE464 pKa = 4.6 EE465 pKa = 4.19 PADD468 pKa = 4.19 LEE470 pKa = 4.37 TVQAVRR476 pKa = 11.84 ILYY479 pKa = 7.83 PHH481 pKa = 6.72 SLLAEE486 pKa = 4.43 EE487 pKa = 5.52 GWQGIQLLAYY497 pKa = 9.3 TEE499 pKa = 4.89 LDD501 pKa = 3.52 DD502 pKa = 6.08 DD503 pKa = 5.19 LEE505 pKa = 5.74 IGQDD509 pKa = 2.68 VWMFGSVFANGVWEE523 pKa = 4.89 GPGEE527 pKa = 4.01 PWDD530 pKa = 4.18 SNVITPTPGTRR541 pKa = 11.84 YY542 pKa = 8.49 PATNGRR548 pKa = 11.84 RR549 pKa = 11.84 DD550 pKa = 3.43 ILRR553 pKa = 11.84 IIYY556 pKa = 7.52 ATPAISKK563 pKa = 10.55 AGDD566 pKa = 3.49 RR567 pKa = 11.84 QVVRR571 pKa = 11.84 PGEE574 pKa = 4.09 PATFTLTYY582 pKa = 9.51 SANGAGAIPDD592 pKa = 3.91 TVDD595 pKa = 2.97 GYY597 pKa = 11.17 EE598 pKa = 4.28 IVDD601 pKa = 3.78 TLPVGMTYY609 pKa = 10.33 VPEE612 pKa = 4.24 STEE615 pKa = 3.95 PEE617 pKa = 4.22 PEE619 pKa = 3.84 VTTNGEE625 pKa = 4.13 GQQVLTWTLNEE636 pKa = 4.37 VPTNTPNTLTYY647 pKa = 10.28 QAVASDD653 pKa = 4.1 SVTPGQTLTNTATSSLAGQSSRR675 pKa = 11.84 PASAQVTVASSGYY688 pKa = 7.86 TLLGKK693 pKa = 8.54 STAQWFMNNPDD704 pKa = 3.67 GEE706 pKa = 4.69 GNSTGSWTVNLRR718 pKa = 11.84 SSDD721 pKa = 4.35 PLPQAYY727 pKa = 8.55 TDD729 pKa = 4.17 TIDD732 pKa = 3.21 ILPYY736 pKa = 10.53 NGDD739 pKa = 3.05 GRR741 pKa = 11.84 GTDD744 pKa = 3.73 FQGTYY749 pKa = 10.58 SVTSVDD755 pKa = 3.75 APAGATVYY763 pKa = 8.88 YY764 pKa = 6.93 TTADD768 pKa = 4.0 RR769 pKa = 11.84 EE770 pKa = 4.42 TLSDD774 pKa = 4.53 DD775 pKa = 3.94 PADD778 pKa = 4.9 EE779 pKa = 4.94 INGAPNAPSDD789 pKa = 3.32 IWSTTPVANPTAIRR803 pKa = 11.84 VIGGQLNPGEE813 pKa = 4.6 AFAFDD818 pKa = 3.79 VNIATEE824 pKa = 4.59 GAAPGDD830 pKa = 3.49 VWVNRR835 pKa = 11.84 AQSRR839 pKa = 11.84 AEE841 pKa = 3.74 HH842 pKa = 5.91 TRR844 pKa = 11.84 LVMRR848 pKa = 11.84 TSEE851 pKa = 4.44 PLTMGTMYY859 pKa = 10.69 SASLKK864 pKa = 10.62 KK865 pKa = 10.48 YY866 pKa = 8.48 VQDD869 pKa = 5.7 RR870 pKa = 11.84 DD871 pKa = 4.47 GEE873 pKa = 4.18 WHH875 pKa = 7.18 DD876 pKa = 4.27 AQDD879 pKa = 3.57 ASDD882 pKa = 4.14 YY883 pKa = 10.51 PVFRR887 pKa = 11.84 IGDD890 pKa = 3.73 EE891 pKa = 3.77 VTYY894 pKa = 10.6 RR895 pKa = 11.84 IGVTNTGQGTLTNVQVTDD913 pKa = 3.83 DD914 pKa = 4.85 KK915 pKa = 11.35 FDD917 pKa = 3.27 QGAFLIEE924 pKa = 4.04 EE925 pKa = 4.59 LAPGDD930 pKa = 3.98 VEE932 pKa = 4.16 YY933 pKa = 11.06 HH934 pKa = 6.77 EE935 pKa = 4.35 YY936 pKa = 10.06 TVTIEE941 pKa = 4.5 DD942 pKa = 3.54 SFGTSFVNTASATADD957 pKa = 3.43 QPEE960 pKa = 4.38 DD961 pKa = 3.88 AEE963 pKa = 5.5 DD964 pKa = 3.7 PVQINSDD971 pKa = 3.67 PAGVEE976 pKa = 3.86 VANYY980 pKa = 10.46 SVVKK984 pKa = 10.18 TSDD987 pKa = 3.22 PSGAAVNPGDD997 pKa = 3.63 VVTYY1001 pKa = 8.55 TVEE1004 pKa = 4.13 VTQEE1008 pKa = 3.73 GSVAAEE1014 pKa = 3.92 ATFSDD1019 pKa = 4.56 DD1020 pKa = 5.16 LSDD1023 pKa = 3.94 VLDD1026 pKa = 4.81 DD1027 pKa = 4.0 ATVDD1031 pKa = 3.33 ADD1033 pKa = 4.58 SIVASTGTAEE1043 pKa = 4.59 LDD1045 pKa = 3.53 GEE1047 pKa = 4.34 ALVWTGTVPVGEE1059 pKa = 4.54 VATVTYY1065 pKa = 9.59 EE1066 pKa = 4.16 AVVKK1070 pKa = 10.67 SYY1072 pKa = 11.62 DD1073 pKa = 3.72 EE1074 pKa = 4.5 IEE1076 pKa = 4.91 ADD1078 pKa = 4.64 GNWILDD1084 pKa = 3.14 NVVFSEE1090 pKa = 4.77 GCAEE1094 pKa = 4.59 EE1095 pKa = 5.58 GDD1097 pKa = 4.47 CNPPEE1102 pKa = 4.04 NPIGKK1107 pKa = 8.33 FTYY1110 pKa = 10.45 SKK1112 pKa = 11.1 VSDD1115 pKa = 4.6 PEE1117 pKa = 4.61 PGTAVAPGQEE1127 pKa = 3.75 IDD1129 pKa = 3.52 YY1130 pKa = 8.81 TITVSQQGEE1139 pKa = 3.97 AAIPGASVVDD1149 pKa = 4.3 DD1150 pKa = 4.73 LSDD1153 pKa = 3.72 VLDD1156 pKa = 5.02 DD1157 pKa = 4.13 ATLDD1161 pKa = 3.75 PATISASAGEE1171 pKa = 4.08 AVLDD1175 pKa = 4.31 GSTLSWTGDD1184 pKa = 3.68 LAVDD1188 pKa = 3.75 DD1189 pKa = 4.91 VVTITYY1195 pKa = 8.13 TVVVVEE1201 pKa = 4.96 DD1202 pKa = 4.45 GSDD1205 pKa = 3.39 EE1206 pKa = 4.16 LVNPVTSEE1214 pKa = 4.12 DD1215 pKa = 3.6 PRR1217 pKa = 11.84 GTCDD1221 pKa = 3.6 EE1222 pKa = 4.28 EE1223 pKa = 4.78 VGCEE1227 pKa = 3.88 TTHH1230 pKa = 6.91 LKK1232 pKa = 10.71 GHH1234 pKa = 5.74 FTYY1237 pKa = 10.91 SKK1239 pKa = 9.45 TADD1242 pKa = 3.87 PGSGATVAEE1251 pKa = 4.44 GDD1253 pKa = 3.96 VITYY1257 pKa = 7.47 TVTVSHH1263 pKa = 6.27 QGKK1266 pKa = 10.09 APLEE1270 pKa = 4.48 DD1271 pKa = 3.25 ATVVDD1276 pKa = 5.22 DD1277 pKa = 4.3 LTDD1280 pKa = 4.07 VIDD1283 pKa = 4.01 DD1284 pKa = 5.24 AIYY1287 pKa = 11.0 NGDD1290 pKa = 3.52 VEE1292 pKa = 4.47 ASSGAVRR1299 pKa = 11.84 YY1300 pKa = 9.76 ADD1302 pKa = 5.38 SKK1304 pKa = 10.16 LTWTGDD1310 pKa = 3.61 LEE1312 pKa = 4.52 VGQVVTFTYY1321 pKa = 9.55 TVEE1324 pKa = 4.17 VTGDD1328 pKa = 3.33 GSDD1331 pKa = 3.42 NLVNPVTTDD1340 pKa = 3.57 DD1341 pKa = 4.03 PRR1343 pKa = 11.84 GTCDD1347 pKa = 3.61 EE1348 pKa = 4.38 AVGCQTEE1355 pKa = 4.72 HH1356 pKa = 7.05 PLEE1359 pKa = 4.68 DD1360 pKa = 4.02 APVPAPGLGGGGTGVGGNLPNTGAPAVLGTLLAGLLMVGAGLTVVARR1407 pKa = 11.84 RR1408 pKa = 11.84 RR1409 pKa = 11.84 RR1410 pKa = 11.84 ISLGKK1415 pKa = 10.25 GQTSSLL1421 pKa = 3.73
Molecular weight: 150.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.503
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.062
Thurlkill 3.541
EMBOSS 3.643
Sillero 3.846
Patrickios 0.973
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A6A9UTD7|A0A6A9UTD7_9ACTN NAD(P)H-binding protein OS=Auraticoccus cholistanensis OX=2656650 GN=GC722_07720 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 THH17 pKa = 6.18 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.87 GRR40 pKa = 11.84 AQLSSS45 pKa = 3.28
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.676
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.398
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3358
0
3358
1128129
28
2244
336.0
35.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.112 ± 0.049
0.724 ± 0.01
5.992 ± 0.038
5.908 ± 0.039
2.597 ± 0.023
9.372 ± 0.039
2.161 ± 0.023
2.618 ± 0.029
1.261 ± 0.022
10.845 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.658 ± 0.018
1.521 ± 0.023
6.193 ± 0.04
3.132 ± 0.025
8.337 ± 0.05
5.19 ± 0.024
5.994 ± 0.036
9.871 ± 0.038
1.658 ± 0.019
1.853 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here