Xanthomonas phage Xp15
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q52PR6|Q52PR6_9CAUD Possible translation initiation factor OS=Xanthomonas phage Xp15 OX=322855 PE=4 SV=1
MM1 pKa = 7.67 SSPDD5 pKa = 3.41 NEE7 pKa = 4.73 LNLHH11 pKa = 6.87 LATEE15 pKa = 4.6 DD16 pKa = 3.51 ASDD19 pKa = 3.98 AKK21 pKa = 10.92 NEE23 pKa = 4.14 RR24 pKa = 11.84 ADD26 pKa = 3.8 EE27 pKa = 5.25 LIQEE31 pKa = 4.21 WMKK34 pKa = 11.18 NPSTVAEE41 pKa = 4.59 WISAVLDD48 pKa = 3.89 EE49 pKa = 4.71 QSQARR54 pKa = 11.84 DD55 pKa = 3.25 DD56 pKa = 4.21 FAALLAEE63 pKa = 5.63 AITVDD68 pKa = 4.61 DD69 pKa = 3.81 YY70 pKa = 12.1 DD71 pKa = 3.58 RR72 pKa = 11.84 CKK74 pKa = 10.6 EE75 pKa = 4.07 ALGEE79 pKa = 4.07 ALDD82 pKa = 4.91 LIEE85 pKa = 4.11 THH87 pKa = 6.43 VMYY90 pKa = 10.73 EE91 pKa = 3.86 MRR93 pKa = 11.84 GDD95 pKa = 3.64 ALAEE99 pKa = 4.34 LEE101 pKa = 4.49 SQSNPLPPEE110 pKa = 4.18 SVDD113 pKa = 5.06 LII115 pKa = 4.3
Molecular weight: 12.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.216
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.617
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.088
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.935
Patrickios 3.516
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.86
Protein with the highest isoelectric point:
>tr|Q52PS9|Q52PS9_9CAUD Uncharacterized protein OS=Xanthomonas phage Xp15 OX=322855 PE=4 SV=1
MM1 pKa = 7.46 PWAPPSARR9 pKa = 11.84 STTSARR15 pKa = 11.84 TATSPTSPPTSSRR28 pKa = 11.84 PKK30 pKa = 9.36 PRR32 pKa = 11.84 RR33 pKa = 11.84 PTASSGG39 pKa = 3.28
Molecular weight: 4.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
17016
26
1339
202.6
22.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.398 ± 0.415
1.046 ± 0.127
5.536 ± 0.157
5.73 ± 0.278
3.873 ± 0.164
7.405 ± 0.359
2.039 ± 0.205
5.959 ± 0.198
5.389 ± 0.354
8.128 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.209 ± 0.187
4.408 ± 0.197
4.402 ± 0.269
3.89 ± 0.131
5.448 ± 0.22
6.858 ± 0.234
6.177 ± 0.254
6.888 ± 0.266
1.628 ± 0.122
3.555 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here