Zea mays (Maize)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 99277 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D6N4N4|A0A1D6N4N4_MAIZE Isoform of A0A1D6N4N1 Formin-like protein OS=Zea mays OX=4577 GN=100192542 PE=3 SV=1
MM1 pKa = 7.63 EE2 pKa = 5.2 LQGQLDD8 pKa = 3.97 MPEE11 pKa = 5.17 AITEE15 pKa = 4.08 DD16 pKa = 3.82 SNIAGFGAATIDD28 pKa = 4.17 GPLHH32 pKa = 6.52 IPDD35 pKa = 5.99 GIQSDD40 pKa = 3.86 AMLPRR45 pKa = 11.84 ANDD48 pKa = 3.47 AYY50 pKa = 10.59 GATAAFSLQVPPNNQVNGASNDD72 pKa = 3.7 FVIGTLFQGVTGPFIGNEE90 pKa = 4.06 KK91 pKa = 9.94 EE92 pKa = 3.82 VALADD97 pKa = 3.99 RR98 pKa = 11.84 EE99 pKa = 4.11 RR100 pKa = 11.84 AQVNTLYY107 pKa = 10.68 SDD109 pKa = 4.09 HH110 pKa = 6.5 FQDD113 pKa = 4.68 VPSDD117 pKa = 3.67 LHH119 pKa = 6.26 STDD122 pKa = 5.03 ANISSQDD129 pKa = 3.34 VTLDD133 pKa = 3.53 NSGQACAHH141 pKa = 6.25 ATDD144 pKa = 3.33 GMTRR148 pKa = 11.84 EE149 pKa = 4.33 LTHH152 pKa = 6.66 FVHH155 pKa = 6.89 SNANIFEE162 pKa = 4.62 SNEE165 pKa = 3.75 VPASEE170 pKa = 4.19 ITGVEE175 pKa = 4.17 YY176 pKa = 11.04 NQGASGFPQSTEE188 pKa = 3.86 DD189 pKa = 3.76 EE190 pKa = 4.33 NAVGYY195 pKa = 9.33 NQDD198 pKa = 2.46 VSGFPRR204 pKa = 11.84 PTEE207 pKa = 4.11 DD208 pKa = 4.33 EE209 pKa = 4.03 NAVDD213 pKa = 4.1 YY214 pKa = 11.32 NQDD217 pKa = 2.53 ASGFSRR223 pKa = 11.84 PTEE226 pKa = 4.11 DD227 pKa = 4.39 EE228 pKa = 4.06 NMVDD232 pKa = 3.89 YY233 pKa = 11.25 NQDD236 pKa = 2.52 ASGFPRR242 pKa = 11.84 PTEE245 pKa = 3.95 DD246 pKa = 3.54 VNAVGYY252 pKa = 9.66 NQDD255 pKa = 2.83 ACVCYY260 pKa = 10.6 GRR262 pKa = 5.05
Molecular weight: 28.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.923
Patrickios 1.189
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A1D6E4Y7|A0A1D6E4Y7_MAIZE Isoform of A0A1D6E4Z0 Uncharacterized protein OS=Zea mays OX=4577 GN=ZEAMMB73_Zm00001d002875 PE=4 SV=1
MM1 pKa = 7.87 ASPLNRR7 pKa = 11.84 PFPQRR12 pKa = 11.84 IRR14 pKa = 11.84 TNPFAPASGRR24 pKa = 11.84 RR25 pKa = 11.84 IHH27 pKa = 6.99 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 KK31 pKa = 8.06 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SGG37 pKa = 3.07
Molecular weight: 4.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.574
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.31
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.231
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
39399
59878
99277
45899859
5
5267
462.3
51.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.253 ± 0.008
1.853 ± 0.004
5.584 ± 0.006
6.318 ± 0.008
3.691 ± 0.005
6.721 ± 0.008
2.518 ± 0.003
4.664 ± 0.006
5.38 ± 0.008
9.546 ± 0.009
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.418 ± 0.003
3.858 ± 0.004
5.314 ± 0.008
3.896 ± 0.006
5.936 ± 0.006
8.757 ± 0.01
4.83 ± 0.005
6.639 ± 0.007
1.201 ± 0.003
2.623 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here