SAR86 cluster bacterium SAR86A

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; SAR86 cluster

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1298 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J5K8A8|J5K8A8_9GAMM Lipoprotein ABC transporter ATP-binding protein OS=SAR86 cluster bacterium SAR86A OX=1123866 GN=lolD PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.56KK3 pKa = 8.76FTLLLTSIFLISACSQSDD21 pKa = 3.53NQNQAEE27 pKa = 4.37TSDD30 pKa = 4.03APPSLVEE37 pKa = 4.14YY38 pKa = 9.11VWHH41 pKa = 6.7KK42 pKa = 10.93AGSEE46 pKa = 3.96FSAEE50 pKa = 3.8NLAMLIRR57 pKa = 11.84SWNGMIDD64 pKa = 3.98DD65 pKa = 4.64AMCNGMMGANILTPEE80 pKa = 4.23VANEE84 pKa = 4.37GYY86 pKa = 11.01DD87 pKa = 4.14FIWVLLWDD95 pKa = 4.12SQSSRR100 pKa = 11.84DD101 pKa = 3.91ACWDD105 pKa = 3.23DD106 pKa = 2.88WTTNLQSNWDD116 pKa = 3.79TMIDD120 pKa = 4.23GIMQYY125 pKa = 11.3DD126 pKa = 4.51LDD128 pKa = 3.96NVYY131 pKa = 11.01LFGWTIGQYY140 pKa = 10.52PKK142 pKa = 10.78VEE144 pKa = 4.12NDD146 pKa = 2.76TGSFVNSFNFCNYY159 pKa = 10.07NEE161 pKa = 5.08GYY163 pKa = 10.16SEE165 pKa = 4.94ADD167 pKa = 3.06LDD169 pKa = 4.1TFRR172 pKa = 11.84DD173 pKa = 4.56DD174 pKa = 3.64IAATNWSDD182 pKa = 3.67SYY184 pKa = 11.12WYY186 pKa = 10.86GLLDD190 pKa = 4.3PKK192 pKa = 10.91FEE194 pKa = 4.64PADD197 pKa = 3.78PAPDD201 pKa = 4.25FVWLDD206 pKa = 3.03LWANSAEE213 pKa = 4.92KK214 pKa = 10.44EE215 pKa = 4.09IAQNKK220 pKa = 8.53YY221 pKa = 10.61NNSDD225 pKa = 3.63LPSTTGAAFTCNNVDD240 pKa = 4.01FSGVAIRR247 pKa = 11.84RR248 pKa = 3.79

Molecular weight: 28.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J4KRQ4|J4KRQ4_9GAMM Methionyl-tRNA formyltransferase OS=SAR86 cluster bacterium SAR86A OX=1123866 GN=fmt PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.99AGRR28 pKa = 11.84AVLSNRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.99KK37 pKa = 9.78GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1298

0

1298

397493

37

1969

306.2

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.029 ± 0.067

0.946 ± 0.021

6.17 ± 0.057

6.848 ± 0.065

4.982 ± 0.055

6.445 ± 0.064

1.627 ± 0.029

8.951 ± 0.072

7.736 ± 0.083

9.199 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.032

6.247 ± 0.058

3.411 ± 0.037

2.77 ± 0.028

3.459 ± 0.042

7.642 ± 0.057

4.662 ± 0.043

5.939 ± 0.062

1.008 ± 0.024

3.434 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski