Amino acid dipepetide frequency for Sphingopyxis sp. (strain 113P3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.004AlaAla: 21.004 ± 0.215	1.081AlaCys: 1.081 ± 0.04	8.122AlaAsp: 8.122 ± 0.149	8.148AlaGlu: 8.148 ± 0.092	4.456AlaPhe: 4.456 ± 0.065	11.561AlaGly: 11.561 ± 0.122	2.489AlaHis: 2.489 ± 0.049	7.016AlaIle: 7.016 ± 0.079	4.332AlaLys: 4.332 ± 0.068	14.063AlaLeu: 14.063 ± 0.139	3.949AlaMet: 3.949 ± 0.068	3.138AlaAsn: 3.138 ± 0.046	6.77AlaPro: 6.77 ± 0.09	4.191AlaGln: 4.191 ± 0.067	10.251AlaArg: 10.251 ± 0.114	6.989AlaSer: 6.989 ± 0.084	6.313AlaThr: 6.313 ± 0.079	8.467AlaVal: 8.467 ± 0.081	1.78AlaTrp: 1.78 ± 0.041	2.629AlaTyr: 2.629 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.046CysAla: 1.046 ± 0.029	0.104CysCys: 0.104 ± 0.01	0.528CysAsp: 0.528 ± 0.024	0.431CysGlu: 0.431 ± 0.018	0.295CysPhe: 0.295 ± 0.014	0.858CysGly: 0.858 ± 0.029	0.222CysHis: 0.222 ± 0.016	0.345CysIle: 0.345 ± 0.016	0.183CysLys: 0.183 ± 0.012	0.694CysLeu: 0.694 ± 0.024	0.14CysMet: 0.14 ± 0.011	0.201CysAsn: 0.201 ± 0.013	0.502CysPro: 0.502 ± 0.018	0.227CysGln: 0.227 ± 0.011	0.632CysArg: 0.632 ± 0.025	0.451CysSer: 0.451 ± 0.021	0.382CysThr: 0.382 ± 0.018	0.492CysVal: 0.492 ± 0.021	0.116CysTrp: 0.116 ± 0.008	0.187CysTyr: 0.187 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.148AspAla: 8.148 ± 0.146	0.453AspCys: 0.453 ± 0.019	3.331AspAsp: 3.331 ± 0.056	3.565AspGlu: 3.565 ± 0.051	2.153AspPhe: 2.153 ± 0.045	5.392AspGly: 5.392 ± 0.072	1.32AspHis: 1.32 ± 0.033	3.106AspIle: 3.106 ± 0.047	1.726AspLys: 1.726 ± 0.037	5.739AspLeu: 5.739 ± 0.065	1.399AspMet: 1.399 ± 0.032	1.397AspAsn: 1.397 ± 0.036	3.871AspPro: 3.871 ± 0.058	1.683AspGln: 1.683 ± 0.034	4.954AspArg: 4.954 ± 0.066	2.318AspSer: 2.318 ± 0.04	2.564AspThr: 2.564 ± 0.047	3.916AspVal: 3.916 ± 0.056	1.149AspTrp: 1.149 ± 0.032	1.678AspTyr: 1.678 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	8.466GluAla: 8.466 ± 0.093	0.368GluCys: 0.368 ± 0.016	2.78GluAsp: 2.78 ± 0.049	3.128GluGlu: 3.128 ± 0.058	1.516GluPhe: 1.516 ± 0.031	4.903GluGly: 4.903 ± 0.07	1.122GluHis: 1.122 ± 0.03	3.091GluIle: 3.091 ± 0.052	2.09GluLys: 2.09 ± 0.049	5.215GluLeu: 5.215 ± 0.061	1.397GluMet: 1.397 ± 0.037	1.378GluAsn: 1.378 ± 0.032	2.665GluPro: 2.665 ± 0.046	1.949GluGln: 1.949 ± 0.038	5.319GluArg: 5.319 ± 0.079	2.223GluSer: 2.223 ± 0.046	3.152GluThr: 3.152 ± 0.048	3.624GluVal: 3.624 ± 0.056	0.869GluTrp: 0.869 ± 0.025	1.02GluTyr: 1.02 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	4.954PheAla: 4.954 ± 0.066	0.348PheCys: 0.348 ± 0.016	2.682PheAsp: 2.682 ± 0.046	2.117PheGlu: 2.117 ± 0.042	1.273PhePhe: 1.273 ± 0.034	3.594PheGly: 3.594 ± 0.06	0.727PheHis: 0.727 ± 0.022	1.488PheIle: 1.488 ± 0.035	0.848PheLys: 0.848 ± 0.025	3.078PheLeu: 3.078 ± 0.049	0.713PheMet: 0.713 ± 0.025	0.968PheAsn: 0.968 ± 0.03	1.567PhePro: 1.567 ± 0.03	0.879PheGln: 0.879 ± 0.025	2.273PheArg: 2.273 ± 0.044	1.979PheSer: 1.979 ± 0.041	1.85PheThr: 1.85 ± 0.038	2.515PheVal: 2.515 ± 0.047	0.514PheTrp: 0.514 ± 0.023	0.893PheTyr: 0.893 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.247GlyAla: 10.247 ± 0.101	0.828GlyCys: 0.828 ± 0.026	5.002GlyAsp: 5.002 ± 0.072	5.267GlyGlu: 5.267 ± 0.073	3.495GlyPhe: 3.495 ± 0.048	8.156GlyGly: 8.156 ± 0.129	1.88GlyHis: 1.88 ± 0.041	4.487GlyIle: 4.487 ± 0.065	3.192GlyLys: 3.192 ± 0.051	8.488GlyLeu: 8.488 ± 0.103	2.174GlyMet: 2.174 ± 0.049	2.174GlyAsn: 2.174 ± 0.052	3.685GlyPro: 3.685 ± 0.055	2.815GlyGln: 2.815 ± 0.046	6.67GlyArg: 6.67 ± 0.081	4.949GlySer: 4.949 ± 0.083	4.557GlyThr: 4.557 ± 0.079	5.965GlyVal: 5.965 ± 0.072	1.646GlyTrp: 1.646 ± 0.037	2.29GlyTyr: 2.29 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.429HisAla: 2.429 ± 0.049	0.245HisCys: 0.245 ± 0.012	1.248HisAsp: 1.248 ± 0.035	0.997HisGlu: 0.997 ± 0.023	0.856HisPhe: 0.856 ± 0.024	1.97HisGly: 1.97 ± 0.042	0.585HisHis: 0.585 ± 0.025	0.945HisIle: 0.945 ± 0.03	0.467HisLys: 0.467 ± 0.019	1.929HisLeu: 1.929 ± 0.041	0.474HisMet: 0.474 ± 0.019	0.465HisAsn: 0.465 ± 0.019	1.337HisPro: 1.337 ± 0.036	0.583HisGln: 0.583 ± 0.023	1.589HisArg: 1.589 ± 0.038	1.006HisSer: 1.006 ± 0.031	0.64HisThr: 0.64 ± 0.022	1.452HisVal: 1.452 ± 0.032	0.388HisTrp: 0.388 ± 0.019	0.586HisTyr: 0.586 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.978IleAla: 7.978 ± 0.079	0.429IleCys: 0.429 ± 0.018	4.027IleAsp: 4.027 ± 0.059	3.623IleGlu: 3.623 ± 0.058	1.696IlePhe: 1.696 ± 0.034	4.933IleGly: 4.933 ± 0.07	0.892IleHis: 0.892 ± 0.028	2.184IleIle: 2.184 ± 0.041	1.214IleLys: 1.214 ± 0.031	4.262IleLeu: 4.262 ± 0.057	0.866IleMet: 0.866 ± 0.024	1.295IleAsn: 1.295 ± 0.035	2.287IlePro: 2.287 ± 0.042	1.05IleGln: 1.05 ± 0.032	3.342IleArg: 3.342 ± 0.054	2.505IleSer: 2.505 ± 0.04	2.445IleThr: 2.445 ± 0.045	4.031IleVal: 4.031 ± 0.059	0.637IleTrp: 0.637 ± 0.02	1.055IleTyr: 1.055 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.302LysAla: 4.302 ± 0.064	0.16LysCys: 0.16 ± 0.011	1.562LysAsp: 1.562 ± 0.037	1.422LysGlu: 1.422 ± 0.04	0.796LysPhe: 0.796 ± 0.026	2.597LysGly: 2.597 ± 0.047	0.544LysHis: 0.544 ± 0.02	1.502LysIle: 1.502 ± 0.038	1.171LysLys: 1.171 ± 0.037	3.128LysLeu: 3.128 ± 0.051	0.762LysMet: 0.762 ± 0.026	0.711LysAsn: 0.711 ± 0.025	1.816LysPro: 1.816 ± 0.04	0.913LysGln: 0.913 ± 0.026	2.436LysArg: 2.436 ± 0.049	1.7LysSer: 1.7 ± 0.031	1.686LysThr: 1.686 ± 0.043	2.066LysVal: 2.066 ± 0.048	0.409LysTrp: 0.409 ± 0.017	0.594LysTyr: 0.594 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.753LeuAla: 14.753 ± 0.153	0.79LeuCys: 0.79 ± 0.026	5.967LeuAsp: 5.967 ± 0.075	4.782LeuGlu: 4.782 ± 0.076	3.689LeuPhe: 3.689 ± 0.073	8.208LeuGly: 8.208 ± 0.087	1.866LeuHis: 1.866 ± 0.039	4.695LeuIle: 4.695 ± 0.057	3.008LeuLys: 3.008 ± 0.047	9.413LeuLeu: 9.413 ± 0.118	2.071LeuMet: 2.071 ± 0.044	2.236LeuAsn: 2.236 ± 0.043	5.499LeuPro: 5.499 ± 0.064	2.371LeuGln: 2.371 ± 0.042	7.016LeuArg: 7.016 ± 0.075	5.966LeuSer: 5.966 ± 0.067	5.491LeuThr: 5.491 ± 0.061	7.029LeuVal: 7.029 ± 0.081	1.265LeuTrp: 1.265 ± 0.032	2.001LeuTyr: 2.001 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	3.183MetAla: 3.183 ± 0.052	0.15MetCys: 0.15 ± 0.012	1.034MetAsp: 1.034 ± 0.032	1.082MetGlu: 1.082 ± 0.029	0.658MetPhe: 0.658 ± 0.021	1.817MetGly: 1.817 ± 0.041	0.43MetHis: 0.43 ± 0.018	1.389MetIle: 1.389 ± 0.034	0.923MetLys: 0.923 ± 0.027	2.584MetLeu: 2.584 ± 0.052	0.636MetMet: 0.636 ± 0.02	0.672MetAsn: 0.672 ± 0.024	1.375MetPro: 1.375 ± 0.032	0.692MetGln: 0.692 ± 0.022	1.854MetArg: 1.854 ± 0.037	1.47MetSer: 1.47 ± 0.035	1.886MetThr: 1.886 ± 0.037	1.482MetVal: 1.482 ± 0.032	0.238MetTrp: 0.238 ± 0.013	0.24MetTyr: 0.24 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.157AsnAla: 3.157 ± 0.059	0.239AsnCys: 0.239 ± 0.014	1.33AsnAsp: 1.33 ± 0.034	1.13AsnGlu: 1.13 ± 0.034	0.963AsnPhe: 0.963 ± 0.03	2.295AsnGly: 2.295 ± 0.052	0.473AsnHis: 0.473 ± 0.019	1.37AsnIle: 1.37 ± 0.033	0.606AsnLys: 0.606 ± 0.024	2.366AsnLeu: 2.366 ± 0.041	0.499AsnMet: 0.499 ± 0.018	0.637AsnAsn: 0.637 ± 0.024	1.698AsnPro: 1.698 ± 0.038	0.736AsnGln: 0.736 ± 0.024	1.903AsnArg: 1.903 ± 0.039	1.21AsnSer: 1.21 ± 0.042	1.017AsnThr: 1.017 ± 0.029	1.69AsnVal: 1.69 ± 0.037	0.432AsnTrp: 0.432 ± 0.019	0.734AsnTyr: 0.734 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	7.436ProAla: 7.436 ± 0.082	0.344ProCys: 0.344 ± 0.016	3.742ProAsp: 3.742 ± 0.06	3.585ProGlu: 3.585 ± 0.056	1.912ProPhe: 1.912 ± 0.037	4.873ProGly: 4.873 ± 0.059	1.05ProHis: 1.05 ± 0.03	2.339ProIle: 2.339 ± 0.047	1.565ProLys: 1.565 ± 0.039	5.06ProLeu: 5.06 ± 0.067	1.238ProMet: 1.238 ± 0.032	1.193ProAsn: 1.193 ± 0.031	3.017ProPro: 3.017 ± 0.065	1.597ProGln: 1.597 ± 0.031	3.314ProArg: 3.314 ± 0.049	2.785ProSer: 2.785 ± 0.048	2.455ProThr: 2.455 ± 0.044	4.305ProVal: 4.305 ± 0.06	0.723ProTrp: 0.723 ± 0.025	1.072ProTyr: 1.072 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.861GlnAla: 3.861 ± 0.06	0.212GlnCys: 0.212 ± 0.013	1.354GlnAsp: 1.354 ± 0.036	1.203GlnGlu: 1.203 ± 0.031	1.076GlnPhe: 1.076 ± 0.029	2.327GlnGly: 2.327 ± 0.042	0.583GlnHis: 0.583 ± 0.021	1.75GlnIle: 1.75 ± 0.032	0.899GlnLys: 0.899 ± 0.025	3.052GlnLeu: 3.052 ± 0.049	0.823GlnMet: 0.823 ± 0.027	0.73GlnAsn: 0.73 ± 0.025	1.714GlnPro: 1.714 ± 0.035	1.166GlnGln: 1.166 ± 0.038	2.512GlnArg: 2.512 ± 0.047	1.713GlnSer: 1.713 ± 0.035	1.485GlnThr: 1.485 ± 0.039	2.019GlnVal: 2.019 ± 0.04	0.454GlnTrp: 0.454 ± 0.018	0.613GlnTyr: 0.613 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	9.409ArgAla: 9.409 ± 0.103	0.556ArgCys: 0.556 ± 0.023	4.417ArgAsp: 4.417 ± 0.068	4.324ArgGlu: 4.324 ± 0.067	3.208ArgPhe: 3.208 ± 0.048	5.397ArgGly: 5.397 ± 0.065	1.832ArgHis: 1.832 ± 0.042	4.273ArgIle: 4.273 ± 0.051	2.246ArgLys: 2.246 ± 0.041	8.421ArgLeu: 8.421 ± 0.092	1.941ArgMet: 1.941 ± 0.036	1.786ArgAsn: 1.786 ± 0.038	3.897ArgPro: 3.897 ± 0.062	2.58ArgGln: 2.58 ± 0.044	6.309ArgArg: 6.309 ± 0.096	3.934ArgSer: 3.934 ± 0.051	3.594ArgThr: 3.594 ± 0.055	4.794ArgVal: 4.794 ± 0.055	1.383ArgTrp: 1.383 ± 0.038	2.002ArgTyr: 2.002 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.671SerAla: 6.671 ± 0.076	0.449SerCys: 0.449 ± 0.017	3.351SerAsp: 3.351 ± 0.05	2.926SerGlu: 2.926 ± 0.052	2.131SerPhe: 2.131 ± 0.04	5.367SerGly: 5.367 ± 0.079	0.987SerHis: 0.987 ± 0.024	2.698SerIle: 2.698 ± 0.049	1.387SerLys: 1.387 ± 0.036	5.248SerLeu: 5.248 ± 0.061	1.183SerMet: 1.183 ± 0.033	1.33SerAsn: 1.33 ± 0.032	2.869SerPro: 2.869 ± 0.044	1.492SerGln: 1.492 ± 0.036	3.646SerArg: 3.646 ± 0.055	2.72SerSer: 2.72 ± 0.05	2.396SerThr: 2.396 ± 0.048	3.619SerVal: 3.619 ± 0.056	0.866SerTrp: 0.866 ± 0.025	1.315SerTyr: 1.315 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.265ThrAla: 6.265 ± 0.081	0.354ThrCys: 0.354 ± 0.016	2.825ThrAsp: 2.825 ± 0.047	2.376ThrGlu: 2.376 ± 0.037	1.594ThrPhe: 1.594 ± 0.033	5.115ThrGly: 5.115 ± 0.075	0.943ThrHis: 0.943 ± 0.026	2.916ThrIle: 2.916 ± 0.055	1.363ThrLys: 1.363 ± 0.033	5.552ThrLeu: 5.552 ± 0.05	1.163ThrMet: 1.163 ± 0.028	1.26ThrAsn: 1.26 ± 0.038	3.33ThrPro: 3.33 ± 0.052	1.397ThrGln: 1.397 ± 0.033	3.556ThrArg: 3.556 ± 0.052	2.691ThrSer: 2.691 ± 0.052	2.487ThrThr: 2.487 ± 0.057	3.661ThrVal: 3.661 ± 0.067	0.578ThrTrp: 0.578 ± 0.021	1.124ThrTyr: 1.124 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.3ValAla: 9.3 ± 0.094	0.532ValCys: 0.532 ± 0.019	4.262ValAsp: 4.262 ± 0.056	4.333ValGlu: 4.333 ± 0.064	2.07ValPhe: 2.07 ± 0.041	5.424ValGly: 5.424 ± 0.077	1.356ValHis: 1.356 ± 0.031	3.555ValIle: 3.555 ± 0.048	1.974ValLys: 1.974 ± 0.043	5.852ValLeu: 5.852 ± 0.073	1.591ValMet: 1.591 ± 0.036	1.839ValAsn: 1.839 ± 0.046	3.898ValPro: 3.898 ± 0.055	1.927ValGln: 1.927 ± 0.042	5.218ValArg: 5.218 ± 0.06	3.831ValSer: 3.831 ± 0.054	4.302ValThr: 4.302 ± 0.066	4.807ValVal: 4.807 ± 0.067	0.865ValTrp: 0.865 ± 0.028	1.36ValTyr: 1.36 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.493TrpAla: 1.493 ± 0.036	0.16TrpCys: 0.16 ± 0.01	0.753TrpAsp: 0.753 ± 0.024	0.645TrpGlu: 0.645 ± 0.021	0.561TrpPhe: 0.561 ± 0.025	1.017TrpGly: 1.017 ± 0.028	0.351TrpHis: 0.351 ± 0.016	0.728TrpIle: 0.728 ± 0.022	0.519TrpLys: 0.519 ± 0.02	1.888TrpLeu: 1.888 ± 0.049	0.352TrpMet: 0.352 ± 0.016	0.462TrpAsn: 0.462 ± 0.019	0.751TrpPro: 0.751 ± 0.023	0.593TrpGln: 0.593 ± 0.02	1.395TrpArg: 1.395 ± 0.036	0.966TrpSer: 0.966 ± 0.029	0.857TrpThr: 0.857 ± 0.027	0.858TrpVal: 0.858 ± 0.025	0.242TrpTrp: 0.242 ± 0.016	0.319TrpTyr: 0.319 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.648TyrAla: 2.648 ± 0.047	0.235TyrCys: 0.235 ± 0.012	1.545TyrAsp: 1.545 ± 0.039	1.19TyrGlu: 1.19 ± 0.031	0.861TyrPhe: 0.861 ± 0.026	2.163TyrGly: 2.163 ± 0.04	0.538TyrHis: 0.538 ± 0.019	0.877TyrIle: 0.877 ± 0.027	0.564TyrLys: 0.564 ± 0.022	2.085TyrLeu: 2.085 ± 0.044	0.423TyrMet: 0.423 ± 0.017	0.61TyrAsn: 0.61 ± 0.027	1.059TyrPro: 1.059 ± 0.029	0.706TyrGln: 0.706 ± 0.026	2.115TyrArg: 2.115 ± 0.041	1.245TyrSer: 1.245 ± 0.035	0.992TyrThr: 0.992 ± 0.03	1.505TyrVal: 1.505 ± 0.034	0.352TyrTrp: 0.352 ± 0.018	0.663TyrTyr: 0.663 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4365 proteins (1371085 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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