Aspergillus thermomutatus
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9702 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A397HML0|A0A397HML0_9EURO Transcription initiation factor IIF subunit alpha OS=Aspergillus thermomutatus OX=41047 GN=CDV56_101474 PE=3 SV=1
MM1 pKa = 7.31 STYY4 pKa = 10.39 QDD6 pKa = 3.41 PSNTRR11 pKa = 11.84 DD12 pKa = 3.44 ALLAALQEE20 pKa = 4.33 QTDD23 pKa = 4.34 LLNAHH28 pKa = 6.79 PEE30 pKa = 3.89 EE31 pKa = 5.84 ADD33 pKa = 3.65 IITKK37 pKa = 10.71 AMDD40 pKa = 4.77 DD41 pKa = 3.42 IKK43 pKa = 11.03 EE44 pKa = 3.84 QLAALEE50 pKa = 4.14 QEE52 pKa = 4.62 EE53 pKa = 4.53 AQDD56 pKa = 4.01 PQAGLSPILEE66 pKa = 4.59 TFTSPQLQTDD76 pKa = 4.71 GFSCPPTPGGYY87 pKa = 10.83 ANDD90 pKa = 4.25 SDD92 pKa = 4.16 TDD94 pKa = 4.05 TNGSDD99 pKa = 4.57 DD100 pKa = 6.17 GEE102 pKa = 5.58 DD103 pKa = 3.6 EE104 pKa = 5.4 LNDD107 pKa = 3.56 HH108 pKa = 6.45 LRR110 pKa = 11.84 EE111 pKa = 3.89 RR112 pKa = 11.84 FCNHH116 pKa = 5.76 EE117 pKa = 4.71 CDD119 pKa = 4.66 HH120 pKa = 6.29 EE121 pKa = 4.68 SCRR124 pKa = 11.84 LANEE128 pKa = 3.73 HH129 pKa = 6.76 WYY131 pKa = 9.8 GCEE134 pKa = 4.01 SQVHH138 pKa = 6.21 GDD140 pKa = 4.14 CDD142 pKa = 4.47 CEE144 pKa = 4.56 DD145 pKa = 4.36 AEE147 pKa = 4.4 TDD149 pKa = 3.69 ADD151 pKa = 4.38 LVCC154 pKa = 5.7
Molecular weight: 16.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.872
Patrickios 0.515
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A397HBG4|A0A397HBG4_9EURO Uncharacterized protein OS=Aspergillus thermomutatus OX=41047 GN=CDV56_101819 PE=4 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 SQSFRR7 pKa = 11.84 LPTQTLTTSLSTPLRR22 pKa = 11.84 TFSSALRR29 pKa = 11.84 SQPTPLTQSLPSFRR43 pKa = 11.84 TSLTSASSLSFGLTQQTRR61 pKa = 11.84 SFSASASLAGRR72 pKa = 11.84 RR73 pKa = 11.84 ATYY76 pKa = 9.91 NPSRR80 pKa = 11.84 RR81 pKa = 11.84 VQKK84 pKa = 10.2 RR85 pKa = 11.84 RR86 pKa = 11.84 HH87 pKa = 5.33 GFLARR92 pKa = 11.84 LRR94 pKa = 11.84 SRR96 pKa = 11.84 GGRR99 pKa = 11.84 KK100 pKa = 8.76 VLQHH104 pKa = 6.17 RR105 pKa = 11.84 RR106 pKa = 11.84 EE107 pKa = 4.03 KK108 pKa = 10.75 GRR110 pKa = 11.84 KK111 pKa = 7.61 SLSWW115 pKa = 3.4
Molecular weight: 12.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 11.096
IPC_protein 12.574
Toseland 12.735
ProMoST 13.232
Dawson 12.735
Bjellqvist 12.735
Wikipedia 13.203
Rodwell 12.325
Grimsley 12.778
Solomon 13.232
Lehninger 13.13
Nozaki 12.735
DTASelect 12.735
Thurlkill 12.735
EMBOSS 13.232
Sillero 12.735
Patrickios 12.047
IPC_peptide 13.232
IPC2_peptide 12.223
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9702
0
9702
5065008
55
6255
522.1
57.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.681 ± 0.019
1.248 ± 0.008
5.634 ± 0.016
6.207 ± 0.025
3.754 ± 0.016
6.741 ± 0.022
2.405 ± 0.01
4.923 ± 0.016
4.569 ± 0.022
9.184 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.132 ± 0.009
3.578 ± 0.014
5.936 ± 0.026
4.056 ± 0.013
6.229 ± 0.022
8.296 ± 0.026
5.876 ± 0.019
6.244 ± 0.016
1.476 ± 0.009
2.832 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here