Amino acid dipepetide frequency for Erythrobacter phage vB_EliS_R6L

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.11AlaAla: 17.11 ± 1.305
0.969AlaCys: 0.969 ± 0.238
10.421AlaAsp: 10.421 ± 0.771
9.694AlaGlu: 9.694 ± 1.133
4.217AlaPhe: 4.217 ± 0.388
10.567AlaGly: 10.567 ± 0.764
1.503AlaHis: 1.503 ± 0.256
6.35AlaIle: 6.35 ± 0.584
5.623AlaLys: 5.623 ± 0.578
10.761AlaLeu: 10.761 ± 0.784
3.926AlaMet: 3.926 ± 0.414
3.441AlaAsn: 3.441 ± 0.467
5.283AlaPro: 5.283 ± 0.469
4.072AlaGln: 4.072 ± 0.582
10.179AlaArg: 10.179 ± 0.884
5.962AlaSer: 5.962 ± 0.543
7.077AlaThr: 7.077 ± 0.666
6.544AlaVal: 6.544 ± 0.614
2.133AlaTrp: 2.133 ± 0.372
2.714AlaTyr: 2.714 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
1.212CysAla: 1.212 ± 0.234
0.145CysCys: 0.145 ± 0.082
0.872CysAsp: 0.872 ± 0.227
0.533CysGlu: 0.533 ± 0.168
0.194CysPhe: 0.194 ± 0.083
1.26CysGly: 1.26 ± 0.299
0.339CysHis: 0.339 ± 0.151
0.194CysIle: 0.194 ± 0.129
0.242CysLys: 0.242 ± 0.099
0.533CysLeu: 0.533 ± 0.144
0.048CysMet: 0.048 ± 0.042
0.388CysAsn: 0.388 ± 0.124
0.679CysPro: 0.679 ± 0.156
0.485CysGln: 0.485 ± 0.156
1.115CysArg: 1.115 ± 0.206
0.388CysSer: 0.388 ± 0.15
0.533CysThr: 0.533 ± 0.149
0.533CysVal: 0.533 ± 0.142
0.097CysTrp: 0.097 ± 0.07
0.194CysTyr: 0.194 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
9.161AspAla: 9.161 ± 0.72
0.339AspCys: 0.339 ± 0.128
6.544AspAsp: 6.544 ± 0.605
4.702AspGlu: 4.702 ± 0.492
2.569AspPhe: 2.569 ± 0.302
6.592AspGly: 6.592 ± 0.524
1.309AspHis: 1.309 ± 0.261
3.296AspIle: 3.296 ± 0.391
1.939AspLys: 1.939 ± 0.332
7.222AspLeu: 7.222 ± 0.585
1.357AspMet: 1.357 ± 0.285
1.26AspAsn: 1.26 ± 0.219
4.653AspPro: 4.653 ± 0.437
3.878AspGln: 3.878 ± 0.461
5.623AspArg: 5.623 ± 0.535
2.424AspSer: 2.424 ± 0.372
2.763AspThr: 2.763 ± 0.412
3.005AspVal: 3.005 ± 0.345
1.842AspTrp: 1.842 ± 0.304
1.551AspTyr: 1.551 ± 0.223
0.0AspXaa: 0.0 ± 0.0
Glu
11.245GluAla: 11.245 ± 1.141
0.582GluCys: 0.582 ± 0.179
3.781GluAsp: 3.781 ± 0.509
4.411GluGlu: 4.411 ± 0.441
1.987GluPhe: 1.987 ± 0.326
4.556GluGly: 4.556 ± 0.544
1.163GluHis: 1.163 ± 0.221
4.556GluIle: 4.556 ± 0.418
2.424GluLys: 2.424 ± 0.463
3.781GluLeu: 3.781 ± 0.496
1.6GluMet: 1.6 ± 0.3
1.987GluAsn: 1.987 ± 0.406
4.217GluPro: 4.217 ± 0.444
3.441GluGln: 3.441 ± 0.387
6.01GluArg: 6.01 ± 0.728
1.842GluSer: 1.842 ± 0.243
2.86GluThr: 2.86 ± 0.433
3.49GluVal: 3.49 ± 0.391
1.551GluTrp: 1.551 ± 0.29
0.872GluTyr: 0.872 ± 0.179
0.0GluXaa: 0.0 ± 0.0
Phe
4.072PheAla: 4.072 ± 0.395
0.242PheCys: 0.242 ± 0.113
3.344PheAsp: 3.344 ± 0.356
2.472PheGlu: 2.472 ± 0.312
0.582PhePhe: 0.582 ± 0.167
2.811PheGly: 2.811 ± 0.402
0.533PheHis: 0.533 ± 0.174
0.921PheIle: 0.921 ± 0.209
0.969PheLys: 0.969 ± 0.199
2.133PheLeu: 2.133 ± 0.356
0.63PheMet: 0.63 ± 0.193
0.776PheAsn: 0.776 ± 0.158
1.842PhePro: 1.842 ± 0.288
0.776PheGln: 0.776 ± 0.186
1.6PheArg: 1.6 ± 0.289
1.6PheSer: 1.6 ± 0.263
1.793PheThr: 1.793 ± 0.286
3.005PheVal: 3.005 ± 0.37
0.872PheTrp: 0.872 ± 0.176
0.679PheTyr: 0.679 ± 0.216
0.0PheXaa: 0.0 ± 0.0
Gly
7.222GlyAla: 7.222 ± 0.746
0.921GlyCys: 0.921 ± 0.205
4.944GlyAsp: 4.944 ± 0.587
5.816GlyGlu: 5.816 ± 0.577
2.617GlyPhe: 2.617 ± 0.335
8.385GlyGly: 8.385 ± 1.012
1.648GlyHis: 1.648 ± 0.315
2.714GlyIle: 2.714 ± 0.379
4.023GlyLys: 4.023 ± 0.471
5.913GlyLeu: 5.913 ± 0.554
2.181GlyMet: 2.181 ± 0.35
3.054GlyAsn: 3.054 ± 0.446
4.314GlyPro: 4.314 ± 0.502
3.248GlyGln: 3.248 ± 0.406
7.222GlyArg: 7.222 ± 0.676
4.217GlySer: 4.217 ± 0.522
5.72GlyThr: 5.72 ± 0.631
6.01GlyVal: 6.01 ± 0.614
2.133GlyTrp: 2.133 ± 0.342
1.842GlyTyr: 1.842 ± 0.336
0.0GlyXaa: 0.0 ± 0.0
His
1.454HisAla: 1.454 ± 0.307
0.194HisCys: 0.194 ± 0.092
0.727HisAsp: 0.727 ± 0.202
0.824HisGlu: 0.824 ± 0.203
0.727HisPhe: 0.727 ± 0.195
1.939HisGly: 1.939 ± 0.305
0.582HisHis: 0.582 ± 0.166
0.969HisIle: 0.969 ± 0.219
0.485HisLys: 0.485 ± 0.15
1.551HisLeu: 1.551 ± 0.27
0.436HisMet: 0.436 ± 0.146
0.339HisAsn: 0.339 ± 0.134
1.163HisPro: 1.163 ± 0.275
0.533HisGln: 0.533 ± 0.16
1.89HisArg: 1.89 ± 0.326
0.872HisSer: 0.872 ± 0.239
0.679HisThr: 0.679 ± 0.17
0.921HisVal: 0.921 ± 0.222
0.291HisTrp: 0.291 ± 0.123
0.339HisTyr: 0.339 ± 0.15
0.0HisXaa: 0.0 ± 0.0
Ile
6.447IleAla: 6.447 ± 0.678
0.679IleCys: 0.679 ± 0.18
4.168IleAsp: 4.168 ± 0.44
3.587IleGlu: 3.587 ± 0.325
1.018IlePhe: 1.018 ± 0.207
4.168IleGly: 4.168 ± 0.504
0.582IleHis: 0.582 ± 0.198
1.842IleIle: 1.842 ± 0.312
1.406IleLys: 1.406 ± 0.249
2.084IleLeu: 2.084 ± 0.306
0.969IleMet: 0.969 ± 0.183
1.309IleAsn: 1.309 ± 0.348
2.133IlePro: 2.133 ± 0.316
0.872IleGln: 0.872 ± 0.222
3.151IleArg: 3.151 ± 0.29
3.151IleSer: 3.151 ± 0.409
2.375IleThr: 2.375 ± 0.333
3.975IleVal: 3.975 ± 0.369
1.115IleTrp: 1.115 ± 0.243
1.551IleTyr: 1.551 ± 0.308
0.0IleXaa: 0.0 ± 0.0
Lys
6.01LysAla: 6.01 ± 0.658
0.339LysCys: 0.339 ± 0.132
1.842LysAsp: 1.842 ± 0.281
1.6LysGlu: 1.6 ± 0.305
0.921LysPhe: 0.921 ± 0.219
2.569LysGly: 2.569 ± 0.428
0.63LysHis: 0.63 ± 0.181
1.696LysIle: 1.696 ± 0.297
1.89LysLys: 1.89 ± 0.463
2.181LysLeu: 2.181 ± 0.344
0.776LysMet: 0.776 ± 0.236
1.066LysAsn: 1.066 ± 0.24
2.569LysPro: 2.569 ± 0.39
1.066LysGln: 1.066 ± 0.188
4.072LysArg: 4.072 ± 0.581
1.939LysSer: 1.939 ± 0.384
1.987LysThr: 1.987 ± 0.342
2.23LysVal: 2.23 ± 0.338
0.582LysTrp: 0.582 ± 0.144
0.339LysTyr: 0.339 ± 0.108
0.0LysXaa: 0.0 ± 0.0
Leu
10.761LeuAla: 10.761 ± 0.82
1.163LeuCys: 1.163 ± 0.236
5.816LeuAsp: 5.816 ± 0.526
4.314LeuGlu: 4.314 ± 0.604
3.49LeuPhe: 3.49 ± 0.53
5.816LeuGly: 5.816 ± 0.571
0.921LeuHis: 0.921 ± 0.168
4.168LeuIle: 4.168 ± 0.452
2.472LeuLys: 2.472 ± 0.404
5.816LeuLeu: 5.816 ± 0.626
2.133LeuMet: 2.133 ± 0.286
3.102LeuAsn: 3.102 ± 0.412
4.072LeuPro: 4.072 ± 0.425
1.89LeuGln: 1.89 ± 0.372
5.671LeuArg: 5.671 ± 0.445
3.975LeuSer: 3.975 ± 0.397
5.526LeuThr: 5.526 ± 0.51
4.023LeuVal: 4.023 ± 0.431
1.357LeuTrp: 1.357 ± 0.318
1.6LeuTyr: 1.6 ± 0.286
0.0LeuXaa: 0.0 ± 0.0
Met
3.975MetAla: 3.975 ± 0.415
0.145MetCys: 0.145 ± 0.083
1.309MetAsp: 1.309 ± 0.275
1.018MetGlu: 1.018 ± 0.236
0.582MetPhe: 0.582 ± 0.188
1.793MetGly: 1.793 ± 0.269
0.388MetHis: 0.388 ± 0.174
1.503MetIle: 1.503 ± 0.217
0.921MetLys: 0.921 ± 0.271
2.278MetLeu: 2.278 ± 0.35
0.63MetMet: 0.63 ± 0.179
0.63MetAsn: 0.63 ± 0.189
1.454MetPro: 1.454 ± 0.294
0.872MetGln: 0.872 ± 0.186
1.793MetArg: 1.793 ± 0.286
1.357MetSer: 1.357 ± 0.256
1.89MetThr: 1.89 ± 0.314
1.115MetVal: 1.115 ± 0.212
0.291MetTrp: 0.291 ± 0.108
0.097MetTyr: 0.097 ± 0.064
0.0MetXaa: 0.0 ± 0.0
Asn
3.393AsnAla: 3.393 ± 0.419
0.291AsnCys: 0.291 ± 0.105
2.375AsnAsp: 2.375 ± 0.328
1.454AsnGlu: 1.454 ± 0.251
0.485AsnPhe: 0.485 ± 0.12
2.957AsnGly: 2.957 ± 0.397
0.436AsnHis: 0.436 ± 0.14
1.357AsnIle: 1.357 ± 0.257
0.872AsnLys: 0.872 ± 0.187
2.133AsnLeu: 2.133 ± 0.3
0.485AsnMet: 0.485 ± 0.19
0.824AsnAsn: 0.824 ± 0.205
1.987AsnPro: 1.987 ± 0.284
1.454AsnGln: 1.454 ± 0.33
2.714AsnArg: 2.714 ± 0.351
1.503AsnSer: 1.503 ± 0.274
1.115AsnThr: 1.115 ± 0.275
1.987AsnVal: 1.987 ± 0.345
0.436AsnTrp: 0.436 ± 0.134
0.436AsnTyr: 0.436 ± 0.131
0.0AsnXaa: 0.0 ± 0.0
Pro
6.931ProAla: 6.931 ± 0.485
0.485ProCys: 0.485 ± 0.151
4.847ProAsp: 4.847 ± 0.486
4.459ProGlu: 4.459 ± 0.579
1.842ProPhe: 1.842 ± 0.293
5.72ProGly: 5.72 ± 0.563
0.824ProHis: 0.824 ± 0.184
1.648ProIle: 1.648 ± 0.269
2.375ProLys: 2.375 ± 0.395
4.556ProLeu: 4.556 ± 0.519
1.551ProMet: 1.551 ± 0.292
1.309ProAsn: 1.309 ± 0.245
4.362ProPro: 4.362 ± 0.587
1.309ProGln: 1.309 ± 0.236
3.926ProArg: 3.926 ± 0.54
2.327ProSer: 2.327 ± 0.413
2.666ProThr: 2.666 ± 0.432
3.199ProVal: 3.199 ± 0.359
1.26ProTrp: 1.26 ± 0.233
1.26ProTyr: 1.26 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
4.556GlnAla: 4.556 ± 0.391
0.388GlnCys: 0.388 ± 0.144
2.133GlnAsp: 2.133 ± 0.345
1.939GlnGlu: 1.939 ± 0.369
1.115GlnPhe: 1.115 ± 0.254
2.763GlnGly: 2.763 ± 0.411
0.679GlnHis: 0.679 ± 0.224
2.617GlnIle: 2.617 ± 0.327
1.357GlnLys: 1.357 ± 0.288
2.617GlnLeu: 2.617 ± 0.549
1.454GlnMet: 1.454 ± 0.282
1.26GlnAsn: 1.26 ± 0.277
1.696GlnPro: 1.696 ± 0.289
1.745GlnGln: 1.745 ± 0.451
2.957GlnArg: 2.957 ± 0.44
1.696GlnSer: 1.696 ± 0.278
2.036GlnThr: 2.036 ± 0.353
1.987GlnVal: 1.987 ± 0.284
0.727GlnTrp: 0.727 ± 0.183
0.679GlnTyr: 0.679 ± 0.193
0.0GlnXaa: 0.0 ± 0.0
Arg
11.051ArgAla: 11.051 ± 0.876
1.357ArgCys: 1.357 ± 0.25
5.186ArgAsp: 5.186 ± 0.501
6.059ArgGlu: 6.059 ± 0.58
2.763ArgPhe: 2.763 ± 0.409
4.314ArgGly: 4.314 ± 0.423
1.745ArgHis: 1.745 ± 0.331
3.296ArgIle: 3.296 ± 0.379
3.054ArgLys: 3.054 ± 0.406
7.028ArgLeu: 7.028 ± 0.64
1.745ArgMet: 1.745 ± 0.274
2.133ArgAsn: 2.133 ± 0.315
4.314ArgPro: 4.314 ± 0.566
3.684ArgGln: 3.684 ± 0.454
7.416ArgArg: 7.416 ± 0.909
3.635ArgSer: 3.635 ± 0.554
3.49ArgThr: 3.49 ± 0.429
5.089ArgVal: 5.089 ± 0.432
2.133ArgTrp: 2.133 ± 0.463
2.181ArgTyr: 2.181 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
4.799SerAla: 4.799 ± 0.419
0.339SerCys: 0.339 ± 0.134
3.49SerAsp: 3.49 ± 0.321
2.666SerGlu: 2.666 ± 0.382
1.745SerPhe: 1.745 ± 0.265
4.992SerGly: 4.992 ± 0.896
0.63SerHis: 0.63 ± 0.153
2.569SerIle: 2.569 ± 0.329
1.309SerLys: 1.309 ± 0.198
3.49SerLeu: 3.49 ± 0.369
1.115SerMet: 1.115 ± 0.242
1.745SerAsn: 1.745 ± 0.343
2.472SerPro: 2.472 ± 0.382
1.696SerGln: 1.696 ± 0.343
3.926SerArg: 3.926 ± 0.47
2.181SerSer: 2.181 ± 0.388
2.908SerThr: 2.908 ± 0.377
3.054SerVal: 3.054 ± 0.351
0.776SerTrp: 0.776 ± 0.186
1.26SerTyr: 1.26 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
6.786ThrAla: 6.786 ± 0.564
0.436ThrCys: 0.436 ± 0.108
3.393ThrAsp: 3.393 ± 0.458
3.732ThrGlu: 3.732 ± 0.419
1.696ThrPhe: 1.696 ± 0.286
4.653ThrGly: 4.653 ± 0.537
1.018ThrHis: 1.018 ± 0.196
3.781ThrIle: 3.781 ± 0.408
2.036ThrLys: 2.036 ± 0.36
4.653ThrLeu: 4.653 ± 0.512
1.066ThrMet: 1.066 ± 0.232
1.648ThrAsn: 1.648 ± 0.318
3.975ThrPro: 3.975 ± 0.482
1.309ThrGln: 1.309 ± 0.257
3.732ThrArg: 3.732 ± 0.407
2.52ThrSer: 2.52 ± 0.441
2.569ThrThr: 2.569 ± 0.411
3.635ThrVal: 3.635 ± 0.634
0.582ThrTrp: 0.582 ± 0.155
1.066ThrTyr: 1.066 ± 0.202
0.0ThrXaa: 0.0 ± 0.0
Val
7.513ValAla: 7.513 ± 0.571
0.582ValCys: 0.582 ± 0.155
4.508ValAsp: 4.508 ± 0.451
4.508ValGlu: 4.508 ± 0.427
1.696ValPhe: 1.696 ± 0.269
5.138ValGly: 5.138 ± 0.528
1.26ValHis: 1.26 ± 0.284
2.133ValIle: 2.133 ± 0.283
1.842ValLys: 1.842 ± 0.28
5.041ValLeu: 5.041 ± 0.538
1.212ValMet: 1.212 ± 0.188
1.503ValAsn: 1.503 ± 0.32
3.393ValPro: 3.393 ± 0.355
2.472ValGln: 2.472 ± 0.461
4.459ValArg: 4.459 ± 0.455
3.296ValSer: 3.296 ± 0.5
3.684ValThr: 3.684 ± 0.439
3.538ValVal: 3.538 ± 0.3
1.066ValTrp: 1.066 ± 0.287
1.066ValTyr: 1.066 ± 0.263
0.0ValXaa: 0.0 ± 0.0
Trp
2.472TrpAla: 2.472 ± 0.443
0.145TrpCys: 0.145 ± 0.109
0.921TrpAsp: 0.921 ± 0.252
0.824TrpGlu: 0.824 ± 0.198
0.727TrpPhe: 0.727 ± 0.22
1.6TrpGly: 1.6 ± 0.26
0.436TrpHis: 0.436 ± 0.134
0.339TrpIle: 0.339 ± 0.149
0.776TrpLys: 0.776 ± 0.18
2.472TrpLeu: 2.472 ± 0.476
0.291TrpMet: 0.291 ± 0.117
0.485TrpAsn: 0.485 ± 0.144
1.212TrpPro: 1.212 ± 0.3
0.872TrpGln: 0.872 ± 0.237
2.036TrpArg: 2.036 ± 0.24
0.872TrpSer: 0.872 ± 0.204
1.6TrpThr: 1.6 ± 0.223
0.969TrpVal: 0.969 ± 0.2
0.533TrpTrp: 0.533 ± 0.182
0.388TrpTyr: 0.388 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.424TyrAla: 2.424 ± 0.34
0.242TyrCys: 0.242 ± 0.127
1.406TyrAsp: 1.406 ± 0.261
1.745TyrGlu: 1.745 ± 0.292
0.679TyrPhe: 0.679 ± 0.191
1.551TyrGly: 1.551 ± 0.288
0.291TyrHis: 0.291 ± 0.135
0.63TyrIle: 0.63 ± 0.179
0.291TyrLys: 0.291 ± 0.103
1.842TyrLeu: 1.842 ± 0.342
0.388TyrMet: 0.388 ± 0.127
0.436TyrAsn: 0.436 ± 0.104
1.066TyrPro: 1.066 ± 0.298
0.727TyrGln: 0.727 ± 0.211
2.181TyrArg: 2.181 ± 0.297
1.406TyrSer: 1.406 ± 0.324
1.018TyrThr: 1.018 ± 0.171
1.6TyrVal: 1.6 ± 0.305
0.194TyrTrp: 0.194 ± 0.087
0.291TyrTyr: 0.291 ± 0.125
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 108 proteins (20632 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski