Amino acid dipepetide frequency for Flavobacterium sp. AG291

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.744AlaAla: 5.744 ± 0.102	0.619AlaCys: 0.619 ± 0.026	3.917AlaAsp: 3.917 ± 0.065	4.5AlaGlu: 4.5 ± 0.066	3.474AlaPhe: 3.474 ± 0.064	5.098AlaGly: 5.098 ± 0.094	1.086AlaHis: 1.086 ± 0.035	5.39AlaIle: 5.39 ± 0.07	4.892AlaLys: 4.892 ± 0.089	6.409AlaLeu: 6.409 ± 0.088	1.683AlaMet: 1.683 ± 0.043	3.924AlaAsn: 3.924 ± 0.076	2.455AlaPro: 2.455 ± 0.064	2.784AlaGln: 2.784 ± 0.042	2.009AlaArg: 2.009 ± 0.05	4.521AlaSer: 4.521 ± 0.082	5.017AlaThr: 5.017 ± 0.185	4.813AlaVal: 4.813 ± 0.073	0.677AlaTrp: 0.677 ± 0.024	2.686AlaTyr: 2.686 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.488CysAla: 0.488 ± 0.023	0.102CysCys: 0.102 ± 0.009	0.487CysAsp: 0.487 ± 0.029	0.468CysGlu: 0.468 ± 0.024	0.429CysPhe: 0.429 ± 0.02	0.724CysGly: 0.724 ± 0.035	0.178CysHis: 0.178 ± 0.014	0.601CysIle: 0.601 ± 0.024	0.404CysLys: 0.404 ± 0.022	0.668CysLeu: 0.668 ± 0.022	0.153CysMet: 0.153 ± 0.011	0.49CysAsn: 0.49 ± 0.028	0.343CysPro: 0.343 ± 0.02	0.217CysGln: 0.217 ± 0.016	0.231CysArg: 0.231 ± 0.014	0.709CysSer: 0.709 ± 0.035	0.586CysThr: 0.586 ± 0.043	0.466CysVal: 0.466 ± 0.02	0.075CysTrp: 0.075 ± 0.009	0.341CysTyr: 0.341 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.082AspAla: 4.082 ± 0.065	0.447AspCys: 0.447 ± 0.021	2.842AspAsp: 2.842 ± 0.055	3.555AspGlu: 3.555 ± 0.06	3.432AspPhe: 3.432 ± 0.056	3.817AspGly: 3.817 ± 0.092	0.834AspHis: 0.834 ± 0.031	4.375AspIle: 4.375 ± 0.067	4.155AspLys: 4.155 ± 0.072	4.567AspLeu: 4.567 ± 0.071	1.323AspMet: 1.323 ± 0.033	3.383AspAsn: 3.383 ± 0.064	1.767AspPro: 1.767 ± 0.05	1.305AspGln: 1.305 ± 0.039	1.876AspArg: 1.876 ± 0.04	3.03AspSer: 3.03 ± 0.055	3.056AspThr: 3.056 ± 0.052	3.657AspVal: 3.657 ± 0.064	0.703AspTrp: 0.703 ± 0.023	2.805AspTyr: 2.805 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.529GluAla: 4.529 ± 0.082	0.398GluCys: 0.398 ± 0.021	3.286GluAsp: 3.286 ± 0.054	4.371GluGlu: 4.371 ± 0.093	2.816GluPhe: 2.816 ± 0.051	3.839GluGly: 3.839 ± 0.063	1.055GluHis: 1.055 ± 0.036	4.909GluIle: 4.909 ± 0.067	5.459GluLys: 5.459 ± 0.093	5.697GluLeu: 5.697 ± 0.088	1.616GluMet: 1.616 ± 0.038	4.147GluAsn: 4.147 ± 0.066	1.688GluPro: 1.688 ± 0.048	2.238GluGln: 2.238 ± 0.051	2.384GluArg: 2.384 ± 0.055	3.058GluSer: 3.058 ± 0.059	3.602GluThr: 3.602 ± 0.066	4.092GluVal: 4.092 ± 0.069	0.681GluTrp: 0.681 ± 0.022	2.511GluTyr: 2.511 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.324PheAla: 3.324 ± 0.062	0.503PheCys: 0.503 ± 0.032	3.168PheAsp: 3.168 ± 0.053	3.175PheGlu: 3.175 ± 0.053	2.535PhePhe: 2.535 ± 0.057	3.478PheGly: 3.478 ± 0.072	0.797PheHis: 0.797 ± 0.028	3.581PheIle: 3.581 ± 0.081	3.21PheLys: 3.21 ± 0.06	4.188PheLeu: 4.188 ± 0.08	1.208PheMet: 1.208 ± 0.032	3.067PheAsn: 3.067 ± 0.054	1.607PhePro: 1.607 ± 0.043	1.263PheGln: 1.263 ± 0.034	1.668PheArg: 1.668 ± 0.041	3.753PheSer: 3.753 ± 0.061	3.646PheThr: 3.646 ± 0.075	3.027PheVal: 3.027 ± 0.054	0.527PheTrp: 0.527 ± 0.021	2.082PheTyr: 2.082 ± 0.051	0.0PheXaa: 0.0 ± 0.0
Gly	4.621GlyAla: 4.621 ± 0.106	0.764GlyCys: 0.764 ± 0.05	3.384GlyAsp: 3.384 ± 0.056	3.599GlyGlu: 3.599 ± 0.058	3.6GlyPhe: 3.6 ± 0.06	4.884GlyGly: 4.884 ± 0.136	1.178GlyHis: 1.178 ± 0.037	5.271GlyIle: 5.271 ± 0.086	4.915GlyLys: 4.915 ± 0.083	5.748GlyLeu: 5.748 ± 0.078	1.706GlyMet: 1.706 ± 0.045	4.028GlyAsn: 4.028 ± 0.083	1.514GlyPro: 1.514 ± 0.061	2.13GlyGln: 2.13 ± 0.047	2.115GlyArg: 2.115 ± 0.053	4.407GlySer: 4.407 ± 0.082	5.057GlyThr: 5.057 ± 0.173	4.445GlyVal: 4.445 ± 0.066	0.802GlyTrp: 0.802 ± 0.03	3.072GlyTyr: 3.072 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.984HisAla: 0.984 ± 0.034	0.166HisCys: 0.166 ± 0.014	0.903HisAsp: 0.903 ± 0.032	1.02HisGlu: 1.02 ± 0.032	1.052HisPhe: 1.052 ± 0.029	1.004HisGly: 1.004 ± 0.032	0.456HisHis: 0.456 ± 0.02	1.354HisIle: 1.354 ± 0.043	1.135HisLys: 1.135 ± 0.034	1.606HisLeu: 1.606 ± 0.039	0.336HisMet: 0.336 ± 0.016	1.001HisAsn: 1.001 ± 0.034	0.803HisPro: 0.803 ± 0.03	0.528HisGln: 0.528 ± 0.022	0.616HisArg: 0.616 ± 0.02	0.972HisSer: 0.972 ± 0.03	1.058HisThr: 1.058 ± 0.034	0.893HisVal: 0.893 ± 0.027	0.186HisTrp: 0.186 ± 0.013	0.867HisTyr: 0.867 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.832IleAla: 5.832 ± 0.074	0.625IleCys: 0.625 ± 0.023	4.441IleAsp: 4.441 ± 0.07	4.778IleGlu: 4.778 ± 0.079	3.239IlePhe: 3.239 ± 0.063	4.607IleGly: 4.607 ± 0.076	1.228IleHis: 1.228 ± 0.032	5.622IleIle: 5.622 ± 0.096	5.266IleLys: 5.266 ± 0.08	6.136IleLeu: 6.136 ± 0.097	1.472IleMet: 1.472 ± 0.037	4.582IleAsn: 4.582 ± 0.064	3.116IlePro: 3.116 ± 0.057	2.268IleGln: 2.268 ± 0.047	2.482IleArg: 2.482 ± 0.054	5.026IleSer: 5.026 ± 0.072	5.381IleThr: 5.381 ± 0.087	4.844IleVal: 4.844 ± 0.068	0.663IleTrp: 0.663 ± 0.028	2.807IleTyr: 2.807 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	5.11LysAla: 5.11 ± 0.086	0.331LysCys: 0.331 ± 0.017	4.328LysAsp: 4.328 ± 0.079	5.545LysGlu: 5.545 ± 0.088	2.716LysPhe: 2.716 ± 0.049	4.492LysGly: 4.492 ± 0.072	1.246LysHis: 1.246 ± 0.034	5.347LysIle: 5.347 ± 0.081	6.445LysLys: 6.445 ± 0.098	6.018LysLeu: 6.018 ± 0.096	2.086LysMet: 2.086 ± 0.041	4.564LysAsn: 4.564 ± 0.072	2.488LysPro: 2.488 ± 0.055	2.628LysGln: 2.628 ± 0.058	2.517LysArg: 2.517 ± 0.054	3.977LysSer: 3.977 ± 0.07	4.401LysThr: 4.401 ± 0.073	4.502LysVal: 4.502 ± 0.066	0.776LysTrp: 0.776 ± 0.027	2.909LysTyr: 2.909 ± 0.058	0.0LysXaa: 0.0 ± 0.0
Leu	6.012LeuAla: 6.012 ± 0.075	0.698LeuCys: 0.698 ± 0.032	4.508LeuAsp: 4.508 ± 0.065	5.33LeuGlu: 5.33 ± 0.097	4.502LeuPhe: 4.502 ± 0.084	5.448LeuGly: 5.448 ± 0.085	1.511LeuHis: 1.511 ± 0.041	6.089LeuIle: 6.089 ± 0.108	6.857LeuLys: 6.857 ± 0.103	8.604LeuLeu: 8.604 ± 0.123	2.09LeuMet: 2.09 ± 0.047	5.313LeuAsn: 5.313 ± 0.082	3.735LeuPro: 3.735 ± 0.064	3.066LeuGln: 3.066 ± 0.054	3.055LeuArg: 3.055 ± 0.056	6.442LeuSer: 6.442 ± 0.077	5.618LeuThr: 5.618 ± 0.097	5.103LeuVal: 5.103 ± 0.071	0.814LeuTrp: 0.814 ± 0.032	3.293LeuTyr: 3.293 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	1.824MetAla: 1.824 ± 0.052	0.133MetCys: 0.133 ± 0.011	1.214MetAsp: 1.214 ± 0.029	1.524MetGlu: 1.524 ± 0.04	0.905MetPhe: 0.905 ± 0.03	1.496MetGly: 1.496 ± 0.037	0.419MetHis: 0.419 ± 0.02	1.48MetIle: 1.48 ± 0.038	2.336MetLys: 2.336 ± 0.051	2.162MetLeu: 2.162 ± 0.047	0.621MetMet: 0.621 ± 0.029	1.255MetAsn: 1.255 ± 0.032	1.048MetPro: 1.048 ± 0.032	0.852MetGln: 0.852 ± 0.03	0.907MetArg: 0.907 ± 0.03	1.424MetSer: 1.424 ± 0.035	1.264MetThr: 1.264 ± 0.039	1.436MetVal: 1.436 ± 0.037	0.169MetTrp: 0.169 ± 0.013	0.751MetTyr: 0.751 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	4.165AsnAla: 4.165 ± 0.063	0.49AsnCys: 0.49 ± 0.033	3.407AsnAsp: 3.407 ± 0.065	3.486AsnGlu: 3.486 ± 0.064	2.896AsnPhe: 2.896 ± 0.059	4.581AsnGly: 4.581 ± 0.108	1.013AsnHis: 1.013 ± 0.033	4.626AsnIle: 4.626 ± 0.066	3.795AsnLys: 3.795 ± 0.065	5.04AsnLeu: 5.04 ± 0.075	1.297AsnMet: 1.297 ± 0.034	4.075AsnAsn: 4.075 ± 0.075	2.915AsnPro: 2.915 ± 0.064	1.845AsnGln: 1.845 ± 0.048	2.059AsnArg: 2.059 ± 0.049	3.588AsnSer: 3.588 ± 0.068	3.886AsnThr: 3.886 ± 0.088	3.82AsnVal: 3.82 ± 0.076	0.736AsnTrp: 0.736 ± 0.027	2.883AsnTyr: 2.883 ± 0.063	0.0AsnXaa: 0.0 ± 0.0
Pro	2.857ProAla: 2.857 ± 0.079	0.223ProCys: 0.223 ± 0.02	2.284ProAsp: 2.284 ± 0.043	2.902ProGlu: 2.902 ± 0.056	1.881ProPhe: 1.881 ± 0.046	2.476ProGly: 2.476 ± 0.064	0.595ProHis: 0.595 ± 0.024	2.295ProIle: 2.295 ± 0.055	2.315ProLys: 2.315 ± 0.048	3.05ProLeu: 3.05 ± 0.057	0.79ProMet: 0.79 ± 0.029	2.126ProAsn: 2.126 ± 0.041	0.966ProPro: 0.966 ± 0.038	1.373ProGln: 1.373 ± 0.046	0.846ProArg: 0.846 ± 0.028	2.26ProSer: 2.26 ± 0.048	2.278ProThr: 2.278 ± 0.094	3.112ProVal: 3.112 ± 0.067	0.31ProTrp: 0.31 ± 0.017	1.53ProTyr: 1.53 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.202GlnAla: 2.202 ± 0.053	0.235GlnCys: 0.235 ± 0.021	1.667GlnAsp: 1.667 ± 0.04	2.134GlnGlu: 2.134 ± 0.044	1.523GlnPhe: 1.523 ± 0.039	1.962GlnGly: 1.962 ± 0.086	0.573GlnHis: 0.573 ± 0.025	2.293GlnIle: 2.293 ± 0.046	2.632GlnLys: 2.632 ± 0.055	3.329GlnLeu: 3.329 ± 0.06	0.84GlnMet: 0.84 ± 0.026	2.105GlnAsn: 2.105 ± 0.044	1.263GlnPro: 1.263 ± 0.047	1.526GlnGln: 1.526 ± 0.048	1.193GlnArg: 1.193 ± 0.033	1.974GlnSer: 1.974 ± 0.054	2.029GlnThr: 2.029 ± 0.062	1.957GlnVal: 1.957 ± 0.042	0.45GlnTrp: 0.45 ± 0.025	1.474GlnTyr: 1.474 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.032ArgAla: 2.032 ± 0.048	0.189ArgCys: 0.189 ± 0.013	1.729ArgAsp: 1.729 ± 0.038	2.19ArgGlu: 2.19 ± 0.05	1.86ArgPhe: 1.86 ± 0.041	1.789ArgGly: 1.789 ± 0.047	0.624ArgHis: 0.624 ± 0.024	2.751ArgIle: 2.751 ± 0.052	2.722ArgLys: 2.722 ± 0.064	3.173ArgLeu: 3.173 ± 0.056	0.976ArgMet: 0.976 ± 0.027	2.086ArgAsn: 2.086 ± 0.045	1.069ArgPro: 1.069 ± 0.033	1.145ArgGln: 1.145 ± 0.035	1.278ArgArg: 1.278 ± 0.046	1.818ArgSer: 1.818 ± 0.042	1.763ArgThr: 1.763 ± 0.042	2.089ArgVal: 2.089 ± 0.046	0.391ArgTrp: 0.391 ± 0.017	1.526ArgTyr: 1.526 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.422SerAla: 4.422 ± 0.078	0.728SerCys: 0.728 ± 0.031	3.347SerAsp: 3.347 ± 0.055	3.595SerGlu: 3.595 ± 0.07	3.701SerPhe: 3.701 ± 0.06	5.104SerGly: 5.104 ± 0.101	1.092SerHis: 1.092 ± 0.034	4.542SerIle: 4.542 ± 0.063	4.019SerLys: 4.019 ± 0.063	5.747SerLeu: 5.747 ± 0.084	1.296SerMet: 1.296 ± 0.034	3.443SerAsn: 3.443 ± 0.068	2.299SerPro: 2.299 ± 0.055	2.156SerGln: 2.156 ± 0.052	2.053SerArg: 2.053 ± 0.046	3.969SerSer: 3.969 ± 0.084	3.681SerThr: 3.681 ± 0.087	4.306SerVal: 4.306 ± 0.055	0.702SerTrp: 0.702 ± 0.027	2.883SerTyr: 2.883 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	5.32ThrAla: 5.32 ± 0.159	0.443ThrCys: 0.443 ± 0.03	3.707ThrAsp: 3.707 ± 0.085	3.621ThrGlu: 3.621 ± 0.059	3.081ThrPhe: 3.081 ± 0.058	5.328ThrGly: 5.328 ± 0.171	1.003ThrHis: 1.003 ± 0.027	5.165ThrIle: 5.165 ± 0.089	3.568ThrLys: 3.568 ± 0.056	5.647ThrLeu: 5.647 ± 0.082	1.06ThrMet: 1.06 ± 0.03	3.508ThrAsn: 3.508 ± 0.101	3.1ThrPro: 3.1 ± 0.084	2.215ThrGln: 2.215 ± 0.081	1.667ThrArg: 1.667 ± 0.038	4.023ThrSer: 4.023 ± 0.098	4.561ThrThr: 4.561 ± 0.144	5.047ThrVal: 5.047 ± 0.155	0.588ThrTrp: 0.588 ± 0.032	2.835ThrTyr: 2.835 ± 0.092	0.0ThrXaa: 0.0 ± 0.0
Val	4.815ValAla: 4.815 ± 0.066	0.595ValCys: 0.595 ± 0.033	3.349ValAsp: 3.349 ± 0.056	3.575ValGlu: 3.575 ± 0.057	3.197ValPhe: 3.197 ± 0.053	3.771ValGly: 3.771 ± 0.064	1.007ValHis: 1.007 ± 0.029	5.032ValIle: 5.032 ± 0.072	4.628ValLys: 4.628 ± 0.073	5.789ValLeu: 5.789 ± 0.079	1.509ValMet: 1.509 ± 0.04	3.96ValAsn: 3.96 ± 0.071	2.484ValPro: 2.484 ± 0.051	1.968ValGln: 1.968 ± 0.046	2.206ValArg: 2.206 ± 0.044	4.585ValSer: 4.585 ± 0.065	4.882ValThr: 4.882 ± 0.161	4.516ValVal: 4.516 ± 0.085	0.664ValTrp: 0.664 ± 0.028	2.709ValTyr: 2.709 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.716TrpAla: 0.716 ± 0.028	0.103TrpCys: 0.103 ± 0.009	0.602TrpAsp: 0.602 ± 0.024	0.626TrpGlu: 0.626 ± 0.025	0.543TrpPhe: 0.543 ± 0.021	0.682TrpGly: 0.682 ± 0.029	0.223TrpHis: 0.223 ± 0.016	0.703TrpIle: 0.703 ± 0.025	0.782TrpLys: 0.782 ± 0.026	0.949TrpLeu: 0.949 ± 0.031	0.28TrpMet: 0.28 ± 0.015	0.69TrpAsn: 0.69 ± 0.025	0.238TrpPro: 0.238 ± 0.014	0.427TrpGln: 0.427 ± 0.021	0.381TrpArg: 0.381 ± 0.019	0.636TrpSer: 0.636 ± 0.033	0.635TrpThr: 0.635 ± 0.031	0.68TrpVal: 0.68 ± 0.032	0.139TrpTrp: 0.139 ± 0.01	0.495TrpTyr: 0.495 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.617TyrAla: 2.617 ± 0.054	0.367TyrCys: 0.367 ± 0.019	2.444TyrAsp: 2.444 ± 0.055	2.38TyrGlu: 2.38 ± 0.048	2.426TyrPhe: 2.426 ± 0.049	2.658TyrGly: 2.658 ± 0.057	0.819TyrHis: 0.819 ± 0.031	2.989TyrIle: 2.989 ± 0.048	2.893TyrLys: 2.893 ± 0.054	3.561TyrLeu: 3.561 ± 0.067	0.878TyrMet: 0.878 ± 0.028	2.855TyrAsn: 2.855 ± 0.06	1.571TyrPro: 1.571 ± 0.038	1.427TyrGln: 1.427 ± 0.043	1.659TyrArg: 1.659 ± 0.036	2.915TyrSer: 2.915 ± 0.069	3.086TyrThr: 3.086 ± 0.09	2.438TyrVal: 2.438 ± 0.054	0.476TyrTrp: 0.476 ± 0.018	2.186TyrTyr: 2.186 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3455 proteins (1150073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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