Ustilaginoidea virens nonsegmented virus 2

Taxonomy: Viruses; Riboviria; dsRNA viruses; unclassified dsRNA viruses

Average proteome isoelectric point is 8.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346P9B8|A0A346P9B8_9VIRU RNA-dependent RNA polymerase OS=Ustilaginoidea virens nonsegmented virus 2 OX=2305465 PE=4 SV=1
MM1 pKa = 7.81HH2 pKa = 7.0YY3 pKa = 9.98HH4 pKa = 6.67LCSPPHH10 pKa = 6.72AMADD14 pKa = 3.17KK15 pKa = 10.83ALQQEE20 pKa = 4.74LEE22 pKa = 4.17LEE24 pKa = 4.3ALVKK28 pKa = 10.75AFNEE32 pKa = 4.12NFKK35 pKa = 8.63TTAGPDD41 pKa = 3.39AVKK44 pKa = 10.64KK45 pKa = 10.76LLGSPHH51 pKa = 4.78VRR53 pKa = 11.84GWYY56 pKa = 9.82AEE58 pKa = 3.85YY59 pKa = 10.27RR60 pKa = 11.84QEE62 pKa = 3.94QAKK65 pKa = 10.45EE66 pKa = 3.75KK67 pKa = 10.63GLPEE71 pKa = 4.04DD72 pKa = 3.34WAAVGLTAQEE82 pKa = 3.69WAEE85 pKa = 4.0AQSARR90 pKa = 11.84SLRR93 pKa = 11.84RR94 pKa = 11.84EE95 pKa = 3.95ARR97 pKa = 11.84AEE99 pKa = 3.87AAKK102 pKa = 9.65TLAAEE107 pKa = 4.49AARR110 pKa = 11.84YY111 pKa = 8.2DD112 pKa = 3.78AAVKK116 pKa = 10.36RR117 pKa = 11.84INATCAAALDD127 pKa = 4.04RR128 pKa = 11.84AAAPMVLALEE138 pKa = 4.65GGYY141 pKa = 9.64EE142 pKa = 4.02QLPLAAQIRR151 pKa = 11.84VEE153 pKa = 4.24SSADD157 pKa = 3.11PEE159 pKa = 4.36ARR161 pKa = 11.84AKK163 pKa = 10.24CLKK166 pKa = 10.8AEE168 pKa = 4.22LADD171 pKa = 4.56ARR173 pKa = 11.84AVWVAKK179 pKa = 10.34ARR181 pKa = 11.84AGEE184 pKa = 4.08APFF187 pKa = 4.75

Molecular weight: 20.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346P9B9|A0A346P9B9_9VIRU Fusion protein OS=Ustilaginoidea virens nonsegmented virus 2 OX=2305465 PE=4 SV=1
MM1 pKa = 8.03SSFRR5 pKa = 11.84WLLRR9 pKa = 11.84SGWNPQRR16 pKa = 11.84TPRR19 pKa = 11.84HH20 pKa = 5.32GRR22 pKa = 11.84SVSKK26 pKa = 10.27PSSRR30 pKa = 11.84THH32 pKa = 5.77VPCGLLKK39 pKa = 10.63PGLARR44 pKa = 11.84LPFSLPMPLDD54 pKa = 4.12GPTWSCPAYY63 pKa = 10.33GLLDD67 pKa = 4.35RR68 pKa = 11.84GTLTAVVSRR77 pKa = 11.84LSRR80 pKa = 11.84KK81 pKa = 5.14WHH83 pKa = 6.34RR84 pKa = 11.84LRR86 pKa = 11.84EE87 pKa = 4.5SVFSRR92 pKa = 11.84VPCDD96 pKa = 3.3SPQSHH101 pKa = 6.36VKK103 pKa = 10.22GQLHH107 pKa = 5.8MFWSARR113 pKa = 11.84LDD115 pKa = 3.42EE116 pKa = 4.62AVEE119 pKa = 3.87HH120 pKa = 6.74RR121 pKa = 11.84EE122 pKa = 4.07TYY124 pKa = 10.86KK125 pKa = 10.79LVQSLPEE132 pKa = 3.69TDD134 pKa = 4.42RR135 pKa = 11.84IRR137 pKa = 11.84MAWKK141 pKa = 9.17YY142 pKa = 10.63LNRR145 pKa = 11.84RR146 pKa = 11.84KK147 pKa = 10.03AQAAKK152 pKa = 10.65ARR154 pKa = 11.84DD155 pKa = 4.0DD156 pKa = 3.68SSGSHH161 pKa = 6.9LPRR164 pKa = 11.84VQAAALWLVLDD175 pKa = 5.03DD176 pKa = 6.05FSLTPARR183 pKa = 11.84ATVLAHH189 pKa = 6.34AEE191 pKa = 3.88YY192 pKa = 10.12RR193 pKa = 11.84RR194 pKa = 11.84KK195 pKa = 10.18RR196 pKa = 11.84FEE198 pKa = 3.71KK199 pKa = 9.99PEE201 pKa = 3.85RR202 pKa = 11.84KK203 pKa = 9.22IRR205 pKa = 11.84TTEE208 pKa = 3.85WVGGAPNPIALTSEE222 pKa = 4.27RR223 pKa = 11.84QVSTKK228 pKa = 7.92WKK230 pKa = 8.81RR231 pKa = 11.84AQSNAEE237 pKa = 3.99RR238 pKa = 11.84LAALCDD244 pKa = 3.98DD245 pKa = 4.63APFPPTAVARR255 pKa = 11.84LDD257 pKa = 3.55GRR259 pKa = 11.84LEE261 pKa = 4.18LPSGCRR267 pKa = 11.84PQKK270 pKa = 10.27FVLPGGPALEE280 pKa = 5.33FIRR283 pKa = 11.84EE284 pKa = 4.01RR285 pKa = 11.84RR286 pKa = 11.84PSLLKK291 pKa = 10.23WLGPSDD297 pKa = 3.78ACVGGSWDD305 pKa = 3.36TSKK308 pKa = 10.7WSFEE312 pKa = 4.39VACQPNKK319 pKa = 10.64GSGHH323 pKa = 6.66AGDD326 pKa = 5.19LYY328 pKa = 10.74RR329 pKa = 11.84ALSSGYY335 pKa = 10.19KK336 pKa = 9.68DD337 pKa = 3.7LKK339 pKa = 10.5LGRR342 pKa = 11.84LPQPSIRR349 pKa = 11.84ALEE352 pKa = 4.1CVKK355 pKa = 10.8VNGRR359 pKa = 11.84AYY361 pKa = 9.83PGIFSSRR368 pKa = 11.84IGNNRR373 pKa = 11.84KK374 pKa = 6.57TAYY377 pKa = 8.98GACVEE382 pKa = 4.37IAKK385 pKa = 9.16QHH387 pKa = 5.67YY388 pKa = 9.57LDD390 pKa = 3.47ARR392 pKa = 11.84EE393 pKa = 4.01RR394 pKa = 11.84FEE396 pKa = 5.48PDD398 pKa = 2.76LSLWACGGRR407 pKa = 11.84GKK409 pKa = 9.64PSQMVQVGDD418 pKa = 3.73RR419 pKa = 11.84LKK421 pKa = 11.06SRR423 pKa = 11.84LILMPEE429 pKa = 4.16TPSALLEE436 pKa = 4.27SAFAQPFTAMLSAVRR451 pKa = 11.84GDD453 pKa = 3.51IMIGASMTDD462 pKa = 2.59RR463 pKa = 11.84GFRR466 pKa = 11.84RR467 pKa = 11.84VLSPVEE473 pKa = 4.22DD474 pKa = 3.46AHH476 pKa = 6.83HH477 pKa = 6.41VKK479 pKa = 10.86AFDD482 pKa = 3.09WSGFDD487 pKa = 3.34SRR489 pKa = 11.84VRR491 pKa = 11.84EE492 pKa = 4.02DD493 pKa = 3.62MIVTAFGIVRR503 pKa = 11.84ACFKK507 pKa = 11.16GDD509 pKa = 4.18DD510 pKa = 3.5AWLDD514 pKa = 3.57NVFLRR519 pKa = 11.84FISHH523 pKa = 6.8FLVKK527 pKa = 10.24RR528 pKa = 11.84VVTPGGWLYY537 pKa = 10.31TLANGVPSGSPFTSIIDD554 pKa = 3.73SLVNWLVITDD564 pKa = 4.85LEE566 pKa = 4.23ICMGGLSAPTKK577 pKa = 9.67NRR579 pKa = 11.84RR580 pKa = 11.84RR581 pKa = 11.84VYY583 pKa = 11.07GDD585 pKa = 4.82DD586 pKa = 3.24FMQAFFAPCLEE597 pKa = 3.94RR598 pKa = 11.84DD599 pKa = 3.62AYY601 pKa = 9.8IALAFEE607 pKa = 4.16RR608 pKa = 11.84WGFVAKK614 pKa = 10.25PSAALEE620 pKa = 4.33GVACANTADD629 pKa = 3.72ASLPFLSFRR638 pKa = 11.84FPFGLPARR646 pKa = 11.84PIQDD650 pKa = 3.28ALKK653 pKa = 10.5IGLLPQKK660 pKa = 10.61ARR662 pKa = 11.84YY663 pKa = 9.16SYY665 pKa = 9.68SAQAARR671 pKa = 11.84VCYY674 pKa = 9.85LDD676 pKa = 4.94HH677 pKa = 6.89FAPYY681 pKa = 10.34DD682 pKa = 3.65PEE684 pKa = 4.26TIEE687 pKa = 3.77YY688 pKa = 9.22HH689 pKa = 6.39RR690 pKa = 11.84EE691 pKa = 3.75YY692 pKa = 11.01FNWLQTKK699 pKa = 9.86IPGMTWADD707 pKa = 3.52GRR709 pKa = 11.84PQPDD713 pKa = 3.87LVSPWIHH720 pKa = 6.0KK721 pKa = 10.85AMVNFVAAGFAPGVVSLGEE740 pKa = 4.0WFRR743 pKa = 11.84QEE745 pKa = 5.18DD746 pKa = 3.67PRR748 pKa = 11.84RR749 pKa = 11.84WPDD752 pKa = 2.29RR753 pKa = 11.84WVPRR757 pKa = 11.84RR758 pKa = 11.84CGRR761 pKa = 11.84LVPRR765 pKa = 11.84AAWSQGKK772 pKa = 8.49LQSALSTLRR781 pKa = 11.84WGNCAEE787 pKa = 4.21STFARR792 pKa = 11.84LRR794 pKa = 11.84WKK796 pKa = 9.11TLL798 pKa = 3.07

Molecular weight: 89.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1923

187

938

641.0

71.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.053 ± 2.626

2.028 ± 0.094

4.992 ± 0.26

5.408 ± 1.087

4.16 ± 0.566

6.448 ± 0.494

1.976 ± 0.043

2.704 ± 0.349

4.784 ± 0.365

9.776 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.716 ± 0.028

1.924 ± 0.072

6.76 ± 0.476

3.276 ± 0.229

9.1 ± 0.518

6.812 ± 0.855

3.744 ± 0.24

5.824 ± 0.216

3.12 ± 0.225

2.392 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski