Rhodococcus sp. SMB37

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2D2J7|A0A4R2D2J7_9NOCA NADPH-dependent sulfite reductase flavoprotein alpha-component OS=Rhodococcus sp. SMB37 OX=2512213 GN=EV641_1182 PE=3 SV=1
MM1 pKa = 7.66ALNKK5 pKa = 10.07KK6 pKa = 9.88AMRR9 pKa = 11.84RR10 pKa = 11.84AVIAASSAALLVLTACGSDD29 pKa = 4.14DD30 pKa = 3.96GGSDD34 pKa = 3.22TGSDD38 pKa = 3.12TGAAASDD45 pKa = 3.97EE46 pKa = 4.1GRR48 pKa = 11.84GPITIVEE55 pKa = 4.33GQDD58 pKa = 3.43PLNAALEE65 pKa = 4.28NIIADD70 pKa = 3.96WNAEE74 pKa = 4.08NPDD77 pKa = 3.49EE78 pKa = 4.35QVTFKK83 pKa = 10.92PLARR87 pKa = 11.84EE88 pKa = 4.33ANDD91 pKa = 3.24QYY93 pKa = 12.07ADD95 pKa = 2.99IAQRR99 pKa = 11.84MQAQSDD105 pKa = 4.27EE106 pKa = 4.39YY107 pKa = 11.43DD108 pKa = 3.61LVAVDD113 pKa = 3.64VTNTAEE119 pKa = 4.07FAANGWLLPLEE130 pKa = 4.51GDD132 pKa = 3.83NAIDD136 pKa = 3.9TDD138 pKa = 4.16GLLPATVEE146 pKa = 3.99SGTYY150 pKa = 10.44NGTLYY155 pKa = 10.8AAPKK159 pKa = 7.51NTNGAFLYY167 pKa = 10.7YY168 pKa = 9.71RR169 pKa = 11.84TDD171 pKa = 3.75LVDD174 pKa = 3.45QAPATWDD181 pKa = 3.43EE182 pKa = 4.75LKK184 pKa = 11.01AEE186 pKa = 4.28CPKK189 pKa = 10.96AEE191 pKa = 4.19EE192 pKa = 4.57AGIACYY198 pKa = 9.96IGQFSNYY205 pKa = 9.48EE206 pKa = 3.97GLTVNVTEE214 pKa = 4.55IVNAFGGSFVAEE226 pKa = 4.84DD227 pKa = 4.29GLTPAIDD234 pKa = 3.54EE235 pKa = 4.26QTAEE239 pKa = 4.14GLQFIVDD246 pKa = 3.93SFEE249 pKa = 5.64DD250 pKa = 3.87GTIPAAAQTYY260 pKa = 8.75TEE262 pKa = 5.06GEE264 pKa = 4.25SQTAFGNGEE273 pKa = 4.26ALFMRR278 pKa = 11.84TWPGFYY284 pKa = 9.75TDD286 pKa = 4.35GEE288 pKa = 4.45EE289 pKa = 4.0TSVAGNYY296 pKa = 9.63GIAVLPGVDD305 pKa = 3.68GPGVSTLGGYY315 pKa = 9.4NVGISAFTDD324 pKa = 3.66APATARR330 pKa = 11.84DD331 pKa = 3.91FLEE334 pKa = 4.71FMQTRR339 pKa = 11.84QSQEE343 pKa = 3.6YY344 pKa = 7.74YY345 pKa = 10.87AEE347 pKa = 4.22VGGAPVLAEE356 pKa = 4.27VYY358 pKa = 10.24DD359 pKa = 4.1DD360 pKa = 4.79PEE362 pKa = 4.43ILEE365 pKa = 4.08QYY367 pKa = 10.45PYY369 pKa = 11.08FSTLKK374 pKa = 10.32EE375 pKa = 3.98ALEE378 pKa = 4.35SAKK381 pKa = 10.02PRR383 pKa = 11.84PSSPYY388 pKa = 8.62YY389 pKa = 10.1SAVSKK394 pKa = 10.81AISDD398 pKa = 3.6NAYY401 pKa = 10.14AAIRR405 pKa = 11.84GEE407 pKa = 4.21KK408 pKa = 9.65PVQQALDD415 pKa = 3.62DD416 pKa = 4.03TQAGIEE422 pKa = 4.18TAGNN426 pKa = 3.39

Molecular weight: 45.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R2D2Y9|A0A4R2D2Y9_9NOCA Uncharacterized protein YlxW (UPF0749 family) OS=Rhodococcus sp. SMB37 OX=2512213 GN=EV641_11866 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVTARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.64GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5840

0

5840

1883713

26

9052

322.6

34.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.426 ± 0.045

0.763 ± 0.01

6.621 ± 0.032

5.758 ± 0.026

3.014 ± 0.017

8.816 ± 0.034

2.285 ± 0.018

4.297 ± 0.023

2.014 ± 0.026

9.865 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.98 ± 0.016

2.024 ± 0.017

5.57 ± 0.026

2.68 ± 0.016

7.372 ± 0.038

5.714 ± 0.021

6.387 ± 0.022

8.926 ± 0.036

1.45 ± 0.013

2.037 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski