Leifsonia xyli subsp. xyli (strain CTCB07)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leifsonia; Leifsonia xyli; Leifsonia xyli subsp. xyli

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6AHG3|Q6AHG3_LEIXX Uncharacterized protein OS=Leifsonia xyli subsp. xyli (strain CTCB07) OX=281090 GN=Lxx00970 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.75PVDD5 pKa = 3.6GWTFLLTSDD14 pKa = 4.72RR15 pKa = 11.84NCPSARR21 pKa = 11.84VVSGFADD28 pKa = 3.7TEE30 pKa = 4.5DD31 pKa = 3.9GDD33 pKa = 4.35TVEE36 pKa = 4.46EE37 pKa = 4.24HH38 pKa = 6.07SQVVSLEE45 pKa = 3.77RR46 pKa = 11.84GVPYY50 pKa = 10.37SYY52 pKa = 10.13TIPDD56 pKa = 3.58SASDD60 pKa = 3.62RR61 pKa = 11.84EE62 pKa = 4.28YY63 pKa = 11.64ASIDD67 pKa = 3.93DD68 pKa = 5.2IICEE72 pKa = 4.37TII74 pKa = 3.12

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6ABX2|Q6ABX2_LEIXX Transcriptional regulator GntR family OS=Leifsonia xyli subsp. xyli (strain CTCB07) OX=281090 GN=gntR PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.25GRR40 pKa = 11.84QKK42 pKa = 11.03LSAA45 pKa = 4.09

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2001

0

2001

593693

20

1980

296.7

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.299 ± 0.078

0.565 ± 0.013

5.631 ± 0.046

5.652 ± 0.065

3.177 ± 0.037

9.065 ± 0.058

1.891 ± 0.025

4.52 ± 0.038

2.435 ± 0.048

10.229 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.746 ± 0.021

2.14 ± 0.029

5.361 ± 0.041

2.895 ± 0.029

7.435 ± 0.061

5.874 ± 0.038

5.993 ± 0.044

8.763 ± 0.052

1.349 ± 0.025

1.981 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski