Ruminococcus sp. CAG:379

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1950 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6ZZ65|R6ZZ65_9FIRM Peptidase family M50 OS=Ruminococcus sp. CAG:379 OX=1262956 GN=BN633_01788 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.25KK3 pKa = 8.79RR4 pKa = 11.84TIFALLAASAAVAAGAAYY22 pKa = 9.69VLSRR26 pKa = 11.84KK27 pKa = 9.23KK28 pKa = 9.99HH29 pKa = 5.11GCCCDD34 pKa = 3.34YY35 pKa = 11.37DD36 pKa = 5.5AGDD39 pKa = 5.14LEE41 pKa = 5.84DD42 pKa = 6.14DD43 pKa = 4.65CDD45 pKa = 4.11CCNCEE50 pKa = 5.21LDD52 pKa = 3.85GDD54 pKa = 4.63CPVEE58 pKa = 4.21DD59 pKa = 4.91LNDD62 pKa = 3.73AEE64 pKa = 4.53MPAVDD69 pKa = 4.47VDD71 pKa = 3.91DD72 pKa = 5.8AEE74 pKa = 4.45TGEE77 pKa = 4.2ATEE80 pKa = 5.34APAEE84 pKa = 4.17DD85 pKa = 5.03APAEE89 pKa = 4.37DD90 pKa = 4.77ADD92 pKa = 3.91KK93 pKa = 11.45SADD96 pKa = 3.43KK97 pKa = 11.15TEE99 pKa = 3.72AA100 pKa = 3.25

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7A5W0|R7A5W0_9FIRM Predicted glycosylase OS=Ruminococcus sp. CAG:379 OX=1262956 GN=BN633_01662 PE=4 SV=1
MM1 pKa = 7.09EE2 pKa = 5.33RR3 pKa = 11.84EE4 pKa = 3.99RR5 pKa = 11.84NSRR8 pKa = 11.84GGKK11 pKa = 9.01RR12 pKa = 11.84PRR14 pKa = 11.84KK15 pKa = 7.88KK16 pKa = 10.22VCMFCVDD23 pKa = 3.43RR24 pKa = 11.84VEE26 pKa = 4.48TIDD29 pKa = 3.59YY30 pKa = 10.79KK31 pKa = 11.25DD32 pKa = 3.04IARR35 pKa = 11.84LRR37 pKa = 11.84KK38 pKa = 10.05CMTDD42 pKa = 2.86RR43 pKa = 11.84AKK45 pKa = 10.39ILPRR49 pKa = 11.84RR50 pKa = 11.84VTGTCAFHH58 pKa = 6.84QRR60 pKa = 11.84EE61 pKa = 4.28LTVAIKK67 pKa = 10.16RR68 pKa = 11.84ARR70 pKa = 11.84HH71 pKa = 5.21IALLPYY77 pKa = 10.32VSEE80 pKa = 4.29

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1950

0

1950

611657

30

2840

313.7

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.94 ± 0.055

1.924 ± 0.03

5.679 ± 0.049

6.245 ± 0.054

3.846 ± 0.039

7.529 ± 0.053

2.008 ± 0.033

6.199 ± 0.049

4.965 ± 0.055

9.594 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.029

3.699 ± 0.047

3.97 ± 0.033

4.086 ± 0.044

5.028 ± 0.061

5.751 ± 0.049

6.017 ± 0.066

6.827 ± 0.043

0.903 ± 0.024

3.911 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski