Amino acid dipepetide frequency for Ruminococcus sp. CAG:379

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.252AlaAla: 10.252 ± 0.213	1.829AlaCys: 1.829 ± 0.062	5.56AlaAsp: 5.56 ± 0.093	5.891AlaGlu: 5.891 ± 0.124	3.567AlaPhe: 3.567 ± 0.091	6.754AlaGly: 6.754 ± 0.119	1.599AlaHis: 1.599 ± 0.049	4.609AlaIle: 4.609 ± 0.092	4.624AlaLys: 4.624 ± 0.098	8.614AlaLeu: 8.614 ± 0.138	2.768AlaMet: 2.768 ± 0.078	2.822AlaAsn: 2.822 ± 0.084	3.146AlaPro: 3.146 ± 0.078	3.876AlaGln: 3.876 ± 0.106	3.732AlaArg: 3.732 ± 0.094	4.558AlaSer: 4.558 ± 0.085	3.726AlaThr: 3.726 ± 0.102	7.275AlaVal: 7.275 ± 0.113	0.777AlaTrp: 0.777 ± 0.036	3.412AlaTyr: 3.412 ± 0.084	0.008AlaXaa: 0.008 ± 0.003
Cys	1.643CysAla: 1.643 ± 0.055	0.459CysCys: 0.459 ± 0.031	1.04CysAsp: 1.04 ± 0.038	0.883CysGlu: 0.883 ± 0.04	0.772CysPhe: 0.772 ± 0.035	2.12CysGly: 2.12 ± 0.079	0.376CysHis: 0.376 ± 0.025	1.216CysIle: 1.216 ± 0.059	0.893CysLys: 0.893 ± 0.044	1.422CysLeu: 1.422 ± 0.052	0.558CysMet: 0.558 ± 0.031	0.667CysAsn: 0.667 ± 0.035	0.891CysPro: 0.891 ± 0.046	0.505CysGln: 0.505 ± 0.03	1.154CysArg: 1.154 ± 0.052	1.205CysSer: 1.205 ± 0.05	1.233CysThr: 1.233 ± 0.048	1.264CysVal: 1.264 ± 0.053	0.175CysTrp: 0.175 ± 0.017	0.765CysTyr: 0.765 ± 0.037	0.002CysXaa: 0.002 ± 0.002
Asp	5.668AspAla: 5.668 ± 0.119	1.056AspCys: 1.056 ± 0.041	2.482AspAsp: 2.482 ± 0.075	3.332AspGlu: 3.332 ± 0.075	2.384AspPhe: 2.384 ± 0.065	4.833AspGly: 4.833 ± 0.119	1.014AspHis: 1.014 ± 0.047	3.482AspIle: 3.482 ± 0.083	2.732AspLys: 2.732 ± 0.072	5.057AspLeu: 5.057 ± 0.097	1.615AspMet: 1.615 ± 0.053	1.914AspAsn: 1.914 ± 0.052	2.743AspPro: 2.743 ± 0.067	1.821AspGln: 1.821 ± 0.055	2.76AspArg: 2.76 ± 0.076	3.262AspSer: 3.262 ± 0.076	3.661AspThr: 3.661 ± 0.076	3.773AspVal: 3.773 ± 0.083	0.629AspTrp: 0.629 ± 0.037	2.568AspTyr: 2.568 ± 0.071	0.002AspXaa: 0.002 ± 0.001
Glu	5.263GluAla: 5.263 ± 0.114	0.7GluCys: 0.7 ± 0.032	3.466GluAsp: 3.466 ± 0.071	4.342GluGlu: 4.342 ± 0.107	1.959GluPhe: 1.959 ± 0.065	3.971GluGly: 3.971 ± 0.085	1.573GluHis: 1.573 ± 0.055	4.3GluIle: 4.3 ± 0.093	3.903GluLys: 3.903 ± 0.1	6.172GluLeu: 6.172 ± 0.105	1.834GluMet: 1.834 ± 0.062	2.941GluAsn: 2.941 ± 0.069	2.291GluPro: 2.291 ± 0.07	3.989GluGln: 3.989 ± 0.105	3.059GluArg: 3.059 ± 0.081	3.02GluSer: 3.02 ± 0.074	3.551GluThr: 3.551 ± 0.085	3.381GluVal: 3.381 ± 0.076	0.492GluTrp: 0.492 ± 0.027	2.241GluTyr: 2.241 ± 0.066	0.0GluXaa: 0.0 ± 0.0
Phe	3.507PheAla: 3.507 ± 0.083	0.907PheCys: 0.907 ± 0.037	2.567PheAsp: 2.567 ± 0.074	2.081PheGlu: 2.081 ± 0.065	1.65PhePhe: 1.65 ± 0.06	3.002PheGly: 3.002 ± 0.065	0.786PheHis: 0.786 ± 0.043	1.932PheIle: 1.932 ± 0.054	1.368PheLys: 1.368 ± 0.052	3.778PheLeu: 3.778 ± 0.095	0.888PheMet: 0.888 ± 0.041	1.254PheAsn: 1.254 ± 0.051	1.395PhePro: 1.395 ± 0.044	1.233PheGln: 1.233 ± 0.05	2.094PheArg: 2.094 ± 0.056	2.855PheSer: 2.855 ± 0.08	2.555PheThr: 2.555 ± 0.063	2.586PheVal: 2.586 ± 0.076	0.376PheTrp: 0.376 ± 0.026	1.643PheTyr: 1.643 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	6.193GlyAla: 6.193 ± 0.124	1.764GlyCys: 1.764 ± 0.07	4.447GlyAsp: 4.447 ± 0.099	4.501GlyGlu: 4.501 ± 0.094	3.262GlyPhe: 3.262 ± 0.081	5.668GlyGly: 5.668 ± 0.126	1.337GlyHis: 1.337 ± 0.051	5.608GlyIle: 5.608 ± 0.122	4.704GlyLys: 4.704 ± 0.097	6.314GlyLeu: 6.314 ± 0.098	2.766GlyMet: 2.766 ± 0.066	2.941GlyAsn: 2.941 ± 0.095	1.095GlyPro: 1.095 ± 0.044	2.454GlyGln: 2.454 ± 0.071	3.214GlyArg: 3.214 ± 0.075	4.547GlySer: 4.547 ± 0.092	5.006GlyThr: 5.006 ± 0.115	5.503GlyVal: 5.503 ± 0.101	0.726GlyTrp: 0.726 ± 0.044	3.235GlyTyr: 3.235 ± 0.076	0.008GlyXaa: 0.008 ± 0.004
His	2.009HisAla: 2.009 ± 0.055	0.502HisCys: 0.502 ± 0.028	0.902HisAsp: 0.902 ± 0.045	0.84HisGlu: 0.84 ± 0.034	0.847HisPhe: 0.847 ± 0.039	1.471HisGly: 1.471 ± 0.047	0.484HisHis: 0.484 ± 0.035	1.357HisIle: 1.357 ± 0.051	0.862HisLys: 0.862 ± 0.04	1.694HisLeu: 1.694 ± 0.063	0.549HisMet: 0.549 ± 0.027	0.806HisAsn: 0.806 ± 0.035	1.133HisPro: 1.133 ± 0.045	0.754HisGln: 0.754 ± 0.034	1.167HisArg: 1.167 ± 0.049	1.131HisSer: 1.131 ± 0.046	1.321HisThr: 1.321 ± 0.042	1.249HisVal: 1.249 ± 0.045	0.186HisTrp: 0.186 ± 0.018	0.813HisTyr: 0.813 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.797IleAla: 5.797 ± 0.106	1.247IleCys: 1.247 ± 0.058	3.422IleAsp: 3.422 ± 0.082	3.1IleGlu: 3.1 ± 0.078	2.057IlePhe: 2.057 ± 0.06	4.8IleGly: 4.8 ± 0.092	1.216IleHis: 1.216 ± 0.05	3.33IleIle: 3.33 ± 0.088	2.598IleLys: 2.598 ± 0.069	6.139IleLeu: 6.139 ± 0.109	1.413IleMet: 1.413 ± 0.047	2.228IleAsn: 2.228 ± 0.061	3.231IlePro: 3.231 ± 0.089	2.3IleGln: 2.3 ± 0.069	4.238IleArg: 4.238 ± 0.1	4.192IleSer: 4.192 ± 0.085	4.071IleThr: 4.071 ± 0.086	3.999IleVal: 3.999 ± 0.084	0.497IleTrp: 0.497 ± 0.03	2.112IleTyr: 2.112 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.346LysAla: 4.346 ± 0.104	0.664LysCys: 0.664 ± 0.037	2.567LysAsp: 2.567 ± 0.063	3.683LysGlu: 3.683 ± 0.09	1.424LysPhe: 1.424 ± 0.054	3.26LysGly: 3.26 ± 0.078	1.058LysHis: 1.058 ± 0.046	3.057LysIle: 3.057 ± 0.074	3.288LysLys: 3.288 ± 0.092	4.859LysLeu: 4.859 ± 0.087	1.375LysMet: 1.375 ± 0.056	2.07LysAsn: 2.07 ± 0.058	2.183LysPro: 2.183 ± 0.068	2.356LysGln: 2.356 ± 0.072	2.804LysArg: 2.804 ± 0.073	2.51LysSer: 2.51 ± 0.073	2.976LysThr: 2.976 ± 0.074	2.848LysVal: 2.848 ± 0.074	0.42LysTrp: 0.42 ± 0.03	1.896LysTyr: 1.896 ± 0.062	0.003LysXaa: 0.003 ± 0.002
Leu	8.246LeuAla: 8.246 ± 0.144	2.302LeuCys: 2.302 ± 0.076	5.322LeuAsp: 5.322 ± 0.098	6.345LeuGlu: 6.345 ± 0.12	3.837LeuPhe: 3.837 ± 0.099	6.546LeuGly: 6.546 ± 0.123	2.166LeuHis: 2.166 ± 0.059	5.519LeuIle: 5.519 ± 0.103	4.21LeuLys: 4.21 ± 0.093	10.257LeuLeu: 10.257 ± 0.204	2.498LeuMet: 2.498 ± 0.068	3.363LeuAsn: 3.363 ± 0.083	4.485LeuPro: 4.485 ± 0.103	3.343LeuGln: 3.343 ± 0.077	5.036LeuArg: 5.036 ± 0.103	6.201LeuSer: 6.201 ± 0.113	6.278LeuThr: 6.278 ± 0.109	5.735LeuVal: 5.735 ± 0.102	0.788LeuTrp: 0.788 ± 0.039	3.455LeuTyr: 3.455 ± 0.075	0.005LeuXaa: 0.005 ± 0.003
Met	2.17MetAla: 2.17 ± 0.067	0.389MetCys: 0.389 ± 0.028	1.532MetAsp: 1.532 ± 0.052	1.964MetGlu: 1.964 ± 0.055	0.855MetPhe: 0.855 ± 0.041	2.068MetGly: 2.068 ± 0.069	0.657MetHis: 0.657 ± 0.036	1.628MetIle: 1.628 ± 0.06	1.635MetLys: 1.635 ± 0.056	3.345MetLeu: 3.345 ± 0.071	0.813MetMet: 0.813 ± 0.039	1.184MetAsn: 1.184 ± 0.047	1.359MetPro: 1.359 ± 0.045	1.637MetGln: 1.637 ± 0.053	1.413MetArg: 1.413 ± 0.05	1.519MetSer: 1.519 ± 0.044	1.808MetThr: 1.808 ± 0.056	1.615MetVal: 1.615 ± 0.067	0.167MetTrp: 0.167 ± 0.017	0.919MetTyr: 0.919 ± 0.037	0.002MetXaa: 0.002 ± 0.002
Asn	3.468AsnAla: 3.468 ± 0.08	0.692AsnCys: 0.692 ± 0.037	1.841AsnAsp: 1.841 ± 0.068	1.944AsnGlu: 1.944 ± 0.073	1.202AsnPhe: 1.202 ± 0.045	3.607AsnGly: 3.607 ± 0.103	0.669AsnHis: 0.669 ± 0.031	2.253AsnIle: 2.253 ± 0.073	1.692AsnLys: 1.692 ± 0.062	3.253AsnLeu: 3.253 ± 0.076	1.015AsnMet: 1.015 ± 0.044	1.385AsnAsn: 1.385 ± 0.056	1.931AsnPro: 1.931 ± 0.061	1.548AsnGln: 1.548 ± 0.051	1.895AsnArg: 1.895 ± 0.059	2.019AsnSer: 2.019 ± 0.067	2.241AsnThr: 2.241 ± 0.067	2.467AsnVal: 2.467 ± 0.069	0.389AsnTrp: 0.389 ± 0.027	1.481AsnTyr: 1.481 ± 0.056	0.002AsnXaa: 0.002 ± 0.002
Pro	3.824ProAla: 3.824 ± 0.097	0.657ProCys: 0.657 ± 0.036	3.134ProAsp: 3.134 ± 0.074	3.87ProGlu: 3.87 ± 0.09	1.573ProPhe: 1.573 ± 0.051	2.805ProGly: 2.805 ± 0.072	0.736ProHis: 0.736 ± 0.042	2.085ProIle: 2.085 ± 0.059	1.811ProLys: 1.811 ± 0.058	3.35ProLeu: 3.35 ± 0.076	1.076ProMet: 1.076 ± 0.04	1.313ProAsn: 1.313 ± 0.046	1.223ProPro: 1.223 ± 0.05	1.579ProGln: 1.579 ± 0.06	1.398ProArg: 1.398 ± 0.051	1.877ProSer: 1.877 ± 0.061	2.009ProThr: 2.009 ± 0.058	3.58ProVal: 3.58 ± 0.079	0.302ProTrp: 0.302 ± 0.022	1.484ProTyr: 1.484 ± 0.053	0.011ProXaa: 0.011 ± 0.005
Gln	3.384GlnAla: 3.384 ± 0.095	0.631GlnCys: 0.631 ± 0.037	2.045GlnAsp: 2.045 ± 0.056	2.624GlnGlu: 2.624 ± 0.077	1.393GlnPhe: 1.393 ± 0.049	2.616GlnGly: 2.616 ± 0.075	0.79GlnHis: 0.79 ± 0.044	2.933GlnIle: 2.933 ± 0.077	2.274GlnLys: 2.274 ± 0.06	4.288GlnLeu: 4.288 ± 0.099	1.179GlnMet: 1.179 ± 0.047	1.563GlnAsn: 1.563 ± 0.057	1.599GlnPro: 1.599 ± 0.057	2.294GlnGln: 2.294 ± 0.081	1.947GlnArg: 1.947 ± 0.074	2.222GlnSer: 2.222 ± 0.077	2.268GlnThr: 2.268 ± 0.053	2.855GlnVal: 2.855 ± 0.066	0.368GlnTrp: 0.368 ± 0.026	1.591GlnTyr: 1.591 ± 0.054	0.0GlnXaa: 0.0 ± 0.0
Arg	3.535ArgAla: 3.535 ± 0.088	0.917ArgCys: 0.917 ± 0.044	2.501ArgAsp: 2.501 ± 0.073	3.361ArgGlu: 3.361 ± 0.097	2.137ArgPhe: 2.137 ± 0.061	2.755ArgGly: 2.755 ± 0.07	1.087ArgHis: 1.087 ± 0.05	4.189ArgIle: 4.189 ± 0.096	2.631ArgLys: 2.631 ± 0.075	5.062ArgLeu: 5.062 ± 0.124	1.744ArgMet: 1.744 ± 0.059	1.903ArgAsn: 1.903 ± 0.049	1.547ArgPro: 1.547 ± 0.045	2.66ArgGln: 2.66 ± 0.089	3.144ArgArg: 3.144 ± 0.101	2.841ArgSer: 2.841 ± 0.071	2.825ArgThr: 2.825 ± 0.062	2.951ArgVal: 2.951 ± 0.069	0.448ArgTrp: 0.448 ± 0.032	2.035ArgTyr: 2.035 ± 0.06	0.008ArgXaa: 0.008 ± 0.004
Ser	5.089SerAla: 5.089 ± 0.096	1.051SerCys: 1.051 ± 0.045	3.435SerAsp: 3.435 ± 0.09	3.047SerGlu: 3.047 ± 0.078	2.5SerPhe: 2.5 ± 0.058	5.459SerGly: 5.459 ± 0.112	1.107SerHis: 1.107 ± 0.043	3.53SerIle: 3.53 ± 0.085	2.652SerLys: 2.652 ± 0.067	5.091SerLeu: 5.091 ± 0.101	1.859SerMet: 1.859 ± 0.059	1.955SerAsn: 1.955 ± 0.068	2.04SerPro: 2.04 ± 0.06	1.973SerGln: 1.973 ± 0.059	2.977SerArg: 2.977 ± 0.076	3.28SerSer: 3.28 ± 0.082	3.119SerThr: 3.119 ± 0.088	4.408SerVal: 4.408 ± 0.1	0.592SerTrp: 0.592 ± 0.029	2.341SerTyr: 2.341 ± 0.072	0.003SerXaa: 0.003 ± 0.002
Thr	5.99ThrAla: 5.99 ± 0.114	0.925ThrCys: 0.925 ± 0.033	3.597ThrAsp: 3.597 ± 0.095	3.999ThrGlu: 3.999 ± 0.085	2.147ThrPhe: 2.147 ± 0.054	5.421ThrGly: 5.421 ± 0.104	0.927ThrHis: 0.927 ± 0.037	3.665ThrIle: 3.665 ± 0.093	2.425ThrLys: 2.425 ± 0.07	5.73ThrLeu: 5.73 ± 0.105	1.594ThrMet: 1.594 ± 0.058	1.932ThrAsn: 1.932 ± 0.073	2.752ThrPro: 2.752 ± 0.061	1.825ThrGln: 1.825 ± 0.055	2.416ThrArg: 2.416 ± 0.06	2.953ThrSer: 2.953 ± 0.081	3.798ThrThr: 3.798 ± 0.222	5.304ThrVal: 5.304 ± 0.109	0.543ThrTrp: 0.543 ± 0.035	2.228ThrTyr: 2.228 ± 0.077	0.002ThrXaa: 0.002 ± 0.001
Val	4.9ValAla: 4.9 ± 0.087	1.599ValCys: 1.599 ± 0.058	3.821ValAsp: 3.821 ± 0.085	3.973ValGlu: 3.973 ± 0.09	2.895ValPhe: 2.895 ± 0.071	4.256ValGly: 4.256 ± 0.089	1.447ValHis: 1.447 ± 0.055	4.455ValIle: 4.455 ± 0.092	3.293ValLys: 3.293 ± 0.085	7.055ValLeu: 7.055 ± 0.13	1.841ValMet: 1.841 ± 0.056	2.645ValAsn: 2.645 ± 0.066	3.034ValPro: 3.034 ± 0.081	2.776ValGln: 2.776 ± 0.073	3.301ValArg: 3.301 ± 0.069	4.556ValSer: 4.556 ± 0.097	4.695ValThr: 4.695 ± 0.117	4.427ValVal: 4.427 ± 0.093	0.638ValTrp: 0.638 ± 0.034	2.657ValTyr: 2.657 ± 0.069	0.003ValXaa: 0.003 ± 0.002
Trp	0.623TrpAla: 0.623 ± 0.036	0.199TrpCys: 0.199 ± 0.019	0.558TrpAsp: 0.558 ± 0.03	0.5TrpGlu: 0.5 ± 0.028	0.412TrpPhe: 0.412 ± 0.028	0.719TrpGly: 0.719 ± 0.037	0.234TrpHis: 0.234 ± 0.021	0.638TrpIle: 0.638 ± 0.032	0.443TrpLys: 0.443 ± 0.027	0.902TrpLeu: 0.902 ± 0.044	0.309TrpMet: 0.309 ± 0.024	0.472TrpAsn: 0.472 ± 0.024	0.141TrpPro: 0.141 ± 0.016	0.402TrpGln: 0.402 ± 0.023	0.376TrpArg: 0.376 ± 0.025	0.546TrpSer: 0.546 ± 0.031	0.479TrpThr: 0.479 ± 0.033	0.53TrpVal: 0.53 ± 0.032	0.118TrpTrp: 0.118 ± 0.013	0.427TrpTyr: 0.427 ± 0.032	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.486TyrAla: 3.486 ± 0.075	0.75TyrCys: 0.75 ± 0.036	2.547TyrAsp: 2.547 ± 0.072	2.163TyrGlu: 2.163 ± 0.06	1.587TyrPhe: 1.587 ± 0.054	3.147TyrGly: 3.147 ± 0.069	0.813TyrHis: 0.813 ± 0.037	2.201TyrIle: 2.201 ± 0.06	1.612TyrLys: 1.612 ± 0.054	3.551TyrLeu: 3.551 ± 0.084	0.979TyrMet: 0.979 ± 0.033	1.632TyrAsn: 1.632 ± 0.059	1.48TyrPro: 1.48 ± 0.049	1.534TyrGln: 1.534 ± 0.062	2.152TyrArg: 2.152 ± 0.053	2.214TyrSer: 2.214 ± 0.079	2.549TyrThr: 2.549 ± 0.08	2.516TyrVal: 2.516 ± 0.059	0.392TyrTrp: 0.392 ± 0.028	1.805TyrTyr: 1.805 ± 0.074	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.007XaaAla: 0.007 ± 0.004	0.0XaaCys: 0.0 ± 0.0	0.003XaaAsp: 0.003 ± 0.002	0.003XaaGlu: 0.003 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.015XaaGly: 0.015 ± 0.005	0.003XaaHis: 0.003 ± 0.002	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.003XaaLeu: 0.003 ± 0.002	0.002XaaMet: 0.002 ± 0.002	0.002XaaAsn: 0.002 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.003XaaGln: 0.003 ± 0.002	0.005XaaArg: 0.005 ± 0.003	0.003XaaSer: 0.003 ± 0.002	0.003XaaThr: 0.003 ± 0.003	0.0XaaVal: 0.0 ± 0.0	0.005XaaTrp: 0.005 ± 0.003	0.0XaaTyr: 0.0 ± 0.0	0.06XaaXaa: 0.06 ± 0.013

Statistics based on 1950 proteins (611657 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---