Pseudarcicella hirudinis
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4993 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I5X0X9|A0A1I5X0X9_9BACT 30S ribosomal protein S6 OS=Pseudarcicella hirudinis OX=1079859 GN=rpsF PE=3 SV=1
MM1 pKa = 7.31 FLQWLIFLPLILPLSIISGAIQGIKK26 pKa = 9.67 SAVYY30 pKa = 10.46 DD31 pKa = 5.09 LINQIQVDD39 pKa = 4.02 VSSQIIHH46 pKa = 7.07 EE47 pKa = 4.83 DD48 pKa = 3.5 SQDD51 pKa = 3.26 SYY53 pKa = 12.13 LL54 pKa = 4.03
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.799
IPC2_protein 4.062
IPC_protein 3.821
Toseland 3.63
ProMoST 4.062
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.884
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.77
Nozaki 4.024
DTASelect 4.24
Thurlkill 3.757
EMBOSS 3.884
Sillero 3.961
Patrickios 1.952
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A1I5WQI8|A0A1I5WQI8_9BACT Peroxiredoxin OS=Pseudarcicella hirudinis OX=1079859 GN=SAMN04515674_112113 PE=4 SV=1
MM1 pKa = 8.02 VLILLLLISNTASFAQCAMCRR22 pKa = 11.84 ATVEE26 pKa = 4.35 STMSNGRR33 pKa = 11.84 NLAATGLNTGILYY46 pKa = 10.48 LLSAPYY52 pKa = 10.39 LIVAGVAFLWYY63 pKa = 9.78 RR64 pKa = 11.84 NSKK67 pKa = 10.61 KK68 pKa = 9.23 EE69 pKa = 3.86 VKK71 pKa = 10.04 RR72 pKa = 11.84 RR73 pKa = 11.84 EE74 pKa = 4.03 NMNLVQRR81 pKa = 11.84 RR82 pKa = 11.84 LNQVLGRR89 pKa = 11.84 II90 pKa = 4.2
Molecular weight: 10.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.882
IPC_protein 10.804
Toseland 10.672
ProMoST 10.482
Dawson 10.804
Bjellqvist 10.584
Wikipedia 11.067
Rodwell 10.935
Grimsley 10.877
Solomon 10.95
Lehninger 10.906
Nozaki 10.687
DTASelect 10.57
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.745
Patrickios 10.716
IPC_peptide 10.95
IPC2_peptide 9.838
IPC2.peptide.svr19 8.241
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4993
0
4993
1789070
29
3529
358.3
40.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.763 ± 0.033
0.84 ± 0.014
5.146 ± 0.024
6.135 ± 0.043
5.237 ± 0.029
6.915 ± 0.034
1.683 ± 0.019
7.325 ± 0.029
7.348 ± 0.035
9.439 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.161 ± 0.019
5.795 ± 0.039
3.789 ± 0.018
3.666 ± 0.021
3.817 ± 0.021
6.862 ± 0.035
5.657 ± 0.043
6.195 ± 0.029
1.243 ± 0.014
3.985 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here