Gordonia phage Cucurbita

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Smoothievirus; unclassified Smoothievirus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3AZN9|A0A1B3AZN9_9CAUD Uncharacterized protein OS=Gordonia phage Cucurbita OX=1887645 GN=115 PE=4 SV=1
MM1 pKa = 7.16TAQGMTIYY9 pKa = 10.73EE10 pKa = 4.44PFTLGRR16 pKa = 11.84LIKK19 pKa = 10.15NLRR22 pKa = 11.84DD23 pKa = 3.71LPPDD27 pKa = 3.48TKK29 pKa = 10.96VRR31 pKa = 11.84GLDD34 pKa = 4.14GIVHH38 pKa = 6.86SYY40 pKa = 9.93RR41 pKa = 11.84GWYY44 pKa = 8.08EE45 pKa = 4.07RR46 pKa = 11.84NATTPTGLVNTAGDD60 pKa = 3.65LADD63 pKa = 5.89FYY65 pKa = 11.42QSDD68 pKa = 3.49IGEE71 pKa = 4.46TMGGWKK77 pKa = 10.39GGDD80 pKa = 3.62YY81 pKa = 10.37IISADD86 pKa = 3.79EE87 pKa = 4.38LIYY90 pKa = 10.88YY91 pKa = 10.15ADD93 pKa = 4.18YY94 pKa = 11.13GDD96 pKa = 4.79SGPLIIGLEE105 pKa = 4.06PTEE108 pKa = 5.27DD109 pKa = 3.74DD110 pKa = 3.51GTYY113 pKa = 10.29TPVLLADD120 pKa = 3.9RR121 pKa = 11.84HH122 pKa = 5.64YY123 pKa = 11.62LLL125 pKa = 5.58

Molecular weight: 13.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3AZQ1|A0A1B3AZQ1_9CAUD MazG-like nucleotide pyrophosphate OS=Gordonia phage Cucurbita OX=1887645 GN=107 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.82SIVAAAEE10 pKa = 3.68SARR13 pKa = 11.84RR14 pKa = 11.84VRR16 pKa = 11.84RR17 pKa = 11.84LVLPGSRR24 pKa = 11.84AWAVIALFTLYY35 pKa = 10.68HH36 pKa = 6.36EE37 pKa = 4.71LRR39 pKa = 11.84CAEE42 pKa = 4.28GEE44 pKa = 4.22LLSEE48 pKa = 4.31AVDD51 pKa = 3.62RR52 pKa = 11.84AIIRR56 pKa = 11.84HH57 pKa = 5.44PVLTRR62 pKa = 11.84LLFAVIMLHH71 pKa = 6.07LNNWIPTKK79 pKa = 10.82LLFLDD84 pKa = 4.88PFHH87 pKa = 6.37QAAVLTKK94 pKa = 10.43HH95 pKa = 5.95LVNRR99 pKa = 4.52

Molecular weight: 11.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

178

0

178

29229

27

2931

164.2

18.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.645 ± 0.37

1.002 ± 0.157

7.092 ± 0.184

6.757 ± 0.195

3.192 ± 0.124

7.893 ± 0.22

2.296 ± 0.168

4.793 ± 0.154

4.078 ± 0.179

7.544 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.088

3.606 ± 0.147

4.865 ± 0.199

3.65 ± 0.244

7.014 ± 0.189

5.645 ± 0.164

5.994 ± 0.204

7.366 ± 0.252

2.172 ± 0.114

2.874 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski