Helicobacter phage Pt5322G

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RGB9|A0A1S5RGB9_9CAUD Uncharacterized protein OS=Helicobacter phage Pt5322G OX=1852679 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.59FIKK30 pKa = 10.97HH31 pKa = 5.29MVDD34 pKa = 2.81KK35 pKa = 10.6TNEE38 pKa = 3.95KK39 pKa = 10.6VEE41 pKa = 4.81DD42 pKa = 3.83LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.13LTDD69 pKa = 3.41KK70 pKa = 10.83VGRR73 pKa = 11.84AMDD76 pKa = 4.45LSDD79 pKa = 4.84EE80 pKa = 4.16IQRR83 pKa = 11.84DD84 pKa = 3.19KK85 pKa = 11.29DD86 pKa = 3.41AEE88 pKa = 4.19EE89 pKa = 4.01IEE91 pKa = 4.13KK92 pKa = 11.03DD93 pKa = 3.3KK94 pKa = 11.44EE95 pKa = 4.14AFLKK99 pKa = 10.2KK100 pKa = 10.22HH101 pKa = 6.49PDD103 pKa = 2.8IDD105 pKa = 4.25FNEE108 pKa = 4.26LLDD111 pKa = 4.15FYY113 pKa = 11.52NEE115 pKa = 3.93EE116 pKa = 3.84VPNRR120 pKa = 11.84FKK122 pKa = 11.17KK123 pKa = 10.62QIDD126 pKa = 3.63KK127 pKa = 11.4LEE129 pKa = 4.21GVAFFEE135 pKa = 6.22AILDD139 pKa = 3.72YY140 pKa = 11.16FNAVNAKK147 pKa = 9.56EE148 pKa = 4.13EE149 pKa = 4.32PKK151 pKa = 10.36SEE153 pKa = 4.06EE154 pKa = 4.06EE155 pKa = 4.17KK156 pKa = 10.94EE157 pKa = 4.27EE158 pKa = 4.01EE159 pKa = 4.18SKK161 pKa = 11.13LPKK164 pKa = 9.77EE165 pKa = 3.94ALGNGVSGVGYY176 pKa = 10.77ANNEE180 pKa = 4.31NIMTRR185 pKa = 11.84YY186 pKa = 9.53

Molecular weight: 21.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RGB7|A0A1S5RGB7_9CAUD Uncharacterized protein OS=Helicobacter phage Pt5322G OX=1852679 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.01PNKK5 pKa = 9.41NLKK8 pKa = 9.5NLNKK12 pKa = 9.66PLEE15 pKa = 4.27KK16 pKa = 10.31RR17 pKa = 11.84SDD19 pKa = 3.38RR20 pKa = 11.84FEE22 pKa = 3.67WCLIIAFSLILATAYY37 pKa = 8.56TLRR40 pKa = 11.84SLLL43 pKa = 3.87

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

9017

43

1969

290.9

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.543 ± 0.338

0.676 ± 0.218

5.19 ± 0.39

8.661 ± 0.305

4.979 ± 0.366

3.992 ± 0.235

1.375 ± 0.144

6.909 ± 0.257

10.957 ± 0.448

10.746 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.83 ± 0.215

8.262 ± 0.425

2.54 ± 0.185

4.614 ± 0.365

3.793 ± 0.226

6.432 ± 0.233

4.536 ± 0.35

4.059 ± 0.4

0.521 ± 0.086

3.382 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski