Streptococcus phage Javan33

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A6V6|A0A4D6A6V6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan33 OX=2548109 GN=Javan33_0035 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.56KK3 pKa = 9.84FDD5 pKa = 4.46HH6 pKa = 6.44YY7 pKa = 10.77FSNHH11 pKa = 4.4IAVKK15 pKa = 10.48NEE17 pKa = 3.67SDD19 pKa = 3.33TVTIVDD25 pKa = 3.76YY26 pKa = 11.02YY27 pKa = 11.14NPEE30 pKa = 3.53KK31 pKa = 10.7GYY33 pKa = 10.57EE34 pKa = 3.95YY35 pKa = 10.53NLRR38 pKa = 11.84YY39 pKa = 10.0IFDD42 pKa = 4.16RR43 pKa = 11.84KK44 pKa = 10.38NSSLAITGDD53 pKa = 3.19FGEE56 pKa = 4.27LVARR60 pKa = 11.84NFYY63 pKa = 11.71NMGEE67 pKa = 3.73WDD69 pKa = 3.76TFYY72 pKa = 11.62NHH74 pKa = 5.37YY75 pKa = 9.2TGHH78 pKa = 7.13LGYY81 pKa = 10.41FLEE84 pKa = 5.22KK85 pKa = 10.0LLCSSRR91 pKa = 11.84PVHH94 pKa = 6.77YY95 pKa = 10.85YY96 pKa = 9.56DD97 pKa = 3.05TDD99 pKa = 3.49KK100 pKa = 11.49AKK102 pKa = 10.84RR103 pKa = 11.84EE104 pKa = 3.89ILNEE108 pKa = 3.87FFDD111 pKa = 4.55GADD114 pKa = 3.6YY115 pKa = 11.51DD116 pKa = 5.72DD117 pKa = 6.25LDD119 pKa = 5.08FDD121 pKa = 6.22DD122 pKa = 6.13EE123 pKa = 4.6FLLDD127 pKa = 3.66QLFDD131 pKa = 4.38NFDD134 pKa = 3.49EE135 pKa = 5.35DD136 pKa = 4.8KK137 pKa = 10.91GFTYY141 pKa = 10.58ILPEE145 pKa = 3.72AFEE148 pKa = 4.52RR149 pKa = 11.84FSEE152 pKa = 4.24DD153 pKa = 3.05MEE155 pKa = 4.28EE156 pKa = 4.67YY157 pKa = 10.11EE158 pKa = 4.42VYY160 pKa = 10.64EE161 pKa = 4.14FLSRR165 pKa = 11.84AGRR168 pKa = 11.84YY169 pKa = 8.77VNDD172 pKa = 3.28VFEE175 pKa = 5.62LYY177 pKa = 10.9LDD179 pKa = 3.97AYY181 pKa = 8.92KK182 pKa = 10.4RR183 pKa = 11.84AYY185 pKa = 9.81EE186 pKa = 4.02YY187 pKa = 9.71LTKK190 pKa = 10.55EE191 pKa = 4.07VDD193 pKa = 4.8DD194 pKa = 4.37GTTQNVV200 pKa = 3.02

Molecular weight: 24.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A7Y2|A0A4D6A7Y2_9CAUD Terminase large subunit OS=Streptococcus phage Javan33 OX=2548109 GN=Javan33_0030 PE=4 SV=1
MM1 pKa = 7.26NEE3 pKa = 3.39VWKK6 pKa = 10.28DD7 pKa = 3.17IPIVKK12 pKa = 10.03GYY14 pKa = 9.6YY15 pKa = 9.03QVSNLGRR22 pKa = 11.84VRR24 pKa = 11.84SIGRR28 pKa = 11.84TVNAKK33 pKa = 9.45QRR35 pKa = 11.84TRR37 pKa = 11.84KK38 pKa = 7.75TKK40 pKa = 10.37GRR42 pKa = 11.84ILKK45 pKa = 10.04QSLSSGYY52 pKa = 10.8AIVTLSVNGLRR63 pKa = 11.84KK64 pKa = 9.89SIRR67 pKa = 11.84VHH69 pKa = 6.18RR70 pKa = 11.84LVAEE74 pKa = 4.09AFIPNPINKK83 pKa = 8.45RR84 pKa = 11.84TINHH88 pKa = 5.94IDD90 pKa = 3.45EE91 pKa = 4.84NKK93 pKa = 10.56LNNRR97 pKa = 11.84VDD99 pKa = 3.71NLEE102 pKa = 3.89WATDD106 pKa = 3.68KK107 pKa = 11.47EE108 pKa = 4.46NANHH112 pKa = 6.87GNRR115 pKa = 11.84TTKK118 pKa = 10.61SSLGRR123 pKa = 11.84CKK125 pKa = 10.1PVEE128 pKa = 3.94QFTLEE133 pKa = 4.11GEE135 pKa = 4.79FINTFDD141 pKa = 4.21SIKK144 pKa = 9.8SASMKK149 pKa = 10.17TGISSQRR156 pKa = 11.84ITATAMGHH164 pKa = 4.95QKK166 pKa = 7.86QTHH169 pKa = 5.47GYY171 pKa = 7.48KK172 pKa = 9.1WRR174 pKa = 11.84YY175 pKa = 7.99AA176 pKa = 3.45

Molecular weight: 20.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10942

42

1224

218.8

24.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.763 ± 0.477

0.557 ± 0.105

6.178 ± 0.255

7.741 ± 0.383

3.921 ± 0.229

6.059 ± 0.463

1.608 ± 0.159

7.074 ± 0.347

8.152 ± 0.4

8.627 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.166

5.812 ± 0.202

2.586 ± 0.142

3.738 ± 0.192

4.725 ± 0.318

6.507 ± 0.483

6.489 ± 0.351

5.867 ± 0.242

1.206 ± 0.139

4.003 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski