Micromonospora sp. (strain ATCC 39149 / NRRL 15099 / SCC 1413)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4RQQ3|C4RQQ3_MICS3 LuxR family transcriptional regulator OS=Micromonospora sp. (strain ATCC 39149 / NRRL 15099 / SCC 1413) OX=219305 GN=MCAG_03905 PE=4 SV=1
MM1 pKa = 5.79TTVRR5 pKa = 11.84VGGTITLTAQWYY17 pKa = 9.43AYY19 pKa = 10.05AGGPAAPVTSVEE31 pKa = 3.83IRR33 pKa = 11.84IAPTGGGAAVVGPTSTGVVAEE54 pKa = 4.01AVGLYY59 pKa = 10.15SYY61 pKa = 11.14AWAVADD67 pKa = 3.72GTTVGDD73 pKa = 4.51YY74 pKa = 10.75VVLWTAVDD82 pKa = 3.67SDD84 pKa = 4.55LEE86 pKa = 4.13AVQASEE92 pKa = 4.5LLTVADD98 pKa = 4.3ALVAGAYY105 pKa = 10.31ASVADD110 pKa = 3.92LTDD113 pKa = 3.45WLGSTPAGAEE123 pKa = 3.81RR124 pKa = 11.84LLVRR128 pKa = 11.84ASRR131 pKa = 11.84DD132 pKa = 2.93VDD134 pKa = 3.87SALLCSVYY142 pKa = 10.74DD143 pKa = 4.11ADD145 pKa = 5.62DD146 pKa = 4.76ADD148 pKa = 3.9VQLALQQATCEE159 pKa = 4.16QVAGMLDD166 pKa = 3.41AGDD169 pKa = 3.98LSGTGVAPASTGFTIGKK186 pKa = 9.86VSVQQGAPGSGSAGGTARR204 pKa = 11.84VGRR207 pKa = 11.84LWYY210 pKa = 9.56QAWLILQTAGLTGQGPQTWW229 pKa = 3.32

Molecular weight: 23.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4RNT6|C4RNT6_MICS3 Acyltransferase 3 OS=Micromonospora sp. (strain ATCC 39149 / NRRL 15099 / SCC 1413) OX=219305 GN=MCAG_01520 PE=4 SV=1
MM1 pKa = 7.42TSHH4 pKa = 6.67GPATGTRR11 pKa = 11.84HH12 pKa = 5.11GQGSGTRR19 pKa = 11.84HH20 pKa = 5.11GQGSGTRR27 pKa = 11.84HH28 pKa = 5.11GQGSGTRR35 pKa = 11.84HH36 pKa = 5.11GQGSGTRR43 pKa = 11.84HH44 pKa = 5.11GQGSGTRR51 pKa = 11.84HH52 pKa = 5.11GQGSGTRR59 pKa = 11.84HH60 pKa = 5.54GPAVAAA66 pKa = 4.69

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5614

0

5614

1848376

45

2637

329.2

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.972 ± 0.049

0.845 ± 0.009

5.963 ± 0.025

4.927 ± 0.03

2.613 ± 0.019

9.609 ± 0.036

2.194 ± 0.018

3.072 ± 0.021

1.621 ± 0.021

10.2 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.621 ± 0.013

1.825 ± 0.021

6.544 ± 0.035

2.692 ± 0.017

8.881 ± 0.044

4.75 ± 0.025

6.086 ± 0.035

8.895 ± 0.03

1.612 ± 0.015

2.078 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski