Vibrio phage SHOU24

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6AT90|W6AT90_9CAUD Uncharacterized protein OS=Vibrio phage SHOU24 OX=1414739 GN=SHOU24_24 PE=4 SV=1
MM1 pKa = 7.22LTDD4 pKa = 3.34TTTTVTGAFSSTRR17 pKa = 11.84SEE19 pKa = 4.11VHH21 pKa = 5.96SGGLVVGSLSNTGSNATAVNGTTTGTVVNTFTEE54 pKa = 5.14TYY56 pKa = 9.77SDD58 pKa = 3.82SNGAYY63 pKa = 9.94SNSSTSTDD71 pKa = 3.37SLSGTNTSAYY81 pKa = 8.19TEE83 pKa = 4.43SYY85 pKa = 9.28TYY87 pKa = 10.72SEE89 pKa = 5.13SYY91 pKa = 10.81VKK93 pKa = 10.81

Molecular weight: 9.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6AT95|W6AT95_9CAUD K1E myramoyl peptidase OS=Vibrio phage SHOU24 OX=1414739 GN=SHOU24_29 PE=4 SV=1
MM1 pKa = 7.59FEE3 pKa = 5.31RR4 pKa = 11.84ITIAGLHH11 pKa = 5.99SSPKK15 pKa = 7.13TFKK18 pKa = 10.15YY19 pKa = 8.92GTIVRR24 pKa = 11.84ILRR27 pKa = 11.84KK28 pKa = 10.1NGAKK32 pKa = 10.16PLDD35 pKa = 3.69LFRR38 pKa = 11.84VEE40 pKa = 4.11RR41 pKa = 11.84EE42 pKa = 3.96MYY44 pKa = 9.01EE45 pKa = 3.91HH46 pKa = 6.93GRR48 pKa = 11.84STFKK52 pKa = 10.99KK53 pKa = 8.95FTLTDD58 pKa = 3.4SSVKK62 pKa = 10.21VLPKK66 pKa = 10.26SNHH69 pKa = 4.31SSRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.04SWLNLPVLTSQQQ86 pKa = 3.06

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

23971

44

1373

249.7

27.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.997 ± 0.475

1.239 ± 0.133

6.374 ± 0.213

7.351 ± 0.364

3.871 ± 0.13

6.449 ± 0.253

2.04 ± 0.161

5.765 ± 0.121

7.046 ± 0.221

8.122 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.662 ± 0.138

4.877 ± 0.118

3.675 ± 0.281

3.554 ± 0.173

4.347 ± 0.203

7.063 ± 0.256

6.02 ± 0.224

7.059 ± 0.248

1.118 ± 0.077

3.371 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski